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Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig
The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promote...
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Published in: | Genes & Genetic Systems 2013, Vol.88(2), pp.135-142 |
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container_title | Genes & Genetic Systems |
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creator | Jung, Yi-Deun Lee, Ja-Rang Kim, Yun-Ji Ha, Hong-Seok Oh, Keon-Bong Im, Gi-Sun Choi, Bong-Hwan Kim, Heui-Soo |
description | The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promoter activities of 3 LTR structures (PERV-LTR1, LTR2, and LTR3 elements) belonging to the PERV-A family were examined using luciferase reporter genes in human liver cell lines (HepG2 and Hep3B). The PERV LTR3 element exhibited hypomethylation and stronger promoter activity than the other LTR elements in human liver cells. We also performed comparative sequences analysis of the PERV LTR elements by using bioinformatics tools. Our findings showed that several transcription factors such as Nkx2-2 and Elk-1 positively influenced the high transcriptional activity of the PERV LTR3 element. |
doi_str_mv | 10.1266/ggs.88.135 |
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Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promoter activities of 3 LTR structures (PERV-LTR1, LTR2, and LTR3 elements) belonging to the PERV-A family were examined using luciferase reporter genes in human liver cell lines (HepG2 and Hep3B). The PERV LTR3 element exhibited hypomethylation and stronger promoter activity than the other LTR elements in human liver cells. We also performed comparative sequences analysis of the PERV LTR elements by using bioinformatics tools. Our findings showed that several transcription factors such as Nkx2-2 and Elk-1 positively influenced the high transcriptional activity of the PERV LTR3 element.</description><identifier>ISSN: 1341-7568</identifier><identifier>EISSN: 1880-5779</identifier><identifier>DOI: 10.1266/ggs.88.135</identifier><identifier>PMID: 23832305</identifier><language>eng ; jpn</language><publisher>Japan: The Genetics Society of Japan</publisher><subject>Animals ; Base Sequence ; DNA Methylation ; Endogenous Retroviruses - genetics ; Hep G2 Cells ; Humans ; long terminal repeat (LTR) ; methylation ; Molecular Sequence Data ; Polymerase Chain Reaction ; Porcine endogenous retrovirus ; porcine endogenous retrovirus (PERV) ; promoter activity ; Promoter Regions, Genetic - genetics ; Sequence Homology, Nucleic Acid ; Swine ; Swine, Miniature ; Terminal Repeat Sequences - genetics ; transcription factor-binding sites ; Transcription, Genetic - genetics ; United States</subject><ispartof>Genes & Genetic Systems, 2013, Vol.88(2), pp.135-142</ispartof><rights>2013 by The Genetics Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c652t-8db72bbaaf10c6579a5e035324c8ef8048ab1733c3177d1c911190f88265e1ca3</citedby><cites>FETCH-LOGICAL-c652t-8db72bbaaf10c6579a5e035324c8ef8048ab1733c3177d1c911190f88265e1ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,870,4043,27956,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23832305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jung, Yi-Deun</creatorcontrib><creatorcontrib>Lee, Ja-Rang</creatorcontrib><creatorcontrib>Kim, Yun-Ji</creatorcontrib><creatorcontrib>Ha, Hong-Seok</creatorcontrib><creatorcontrib>Oh, Keon-Bong</creatorcontrib><creatorcontrib>Im, Gi-Sun</creatorcontrib><creatorcontrib>Choi, Bong-Hwan</creatorcontrib><creatorcontrib>Kim, Heui-Soo</creatorcontrib><creatorcontrib>National Livestock Research Institute</creatorcontrib><creatorcontrib>Department of Biological Sciences</creatorcontrib><creatorcontrib>Pusan National University</creatorcontrib><creatorcontrib>Dankook University</creatorcontrib><creatorcontrib>Animal Genomics & Bioinformatics Division</creatorcontrib><creatorcontrib>RDA</creatorcontrib><creatorcontrib>College of Natural Sciences</creatorcontrib><creatorcontrib>National Institute of Animal Science</creatorcontrib><creatorcontrib>Department of Nanobiomedical Science & WCU Research Center</creatorcontrib><creatorcontrib>Animal Biotechnology Division</creatorcontrib><title>Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig</title><title>Genes & Genetic Systems</title><addtitle>Genes Genet. Syst.</addtitle><description>The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promoter activities of 3 LTR structures (PERV-LTR1, LTR2, and LTR3 elements) belonging to the PERV-A family were examined using luciferase reporter genes in human liver cell lines (HepG2 and Hep3B). The PERV LTR3 element exhibited hypomethylation and stronger promoter activity than the other LTR elements in human liver cells. We also performed comparative sequences analysis of the PERV LTR elements by using bioinformatics tools. Our findings showed that several transcription factors such as Nkx2-2 and Elk-1 positively influenced the high transcriptional activity of the PERV LTR3 element.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>DNA Methylation</subject><subject>Endogenous Retroviruses - genetics</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>long terminal repeat (LTR)</subject><subject>methylation</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Porcine endogenous retrovirus</subject><subject>porcine endogenous retrovirus (PERV)</subject><subject>promoter activity</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Terminal Repeat Sequences - genetics</subject><subject>transcription factor-binding sites</subject><subject>Transcription, Genetic - genetics</subject><subject>United States</subject><issn>1341-7568</issn><issn>1880-5779</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkU2LFDEQhhtR3HX14g-QgBcReqwknU5y8LAs6-7CoMuyeg3pTHomQ3-MSUZof7019OwIXrykPvLwVlFvUbylsKCsrj-t12mh1IJy8aw4p0pBKaTUzzHnFS2lqNVZ8SqlLQADrfjL4oxxxRkHcV6E-zj2Y_aRWJfDr5AnYgfbTSkkTFak93kzdTaHcSBuYyNSPobfc2NsyfLxgfjO937I6VDfXz_8SCQM5OvdLenDEGzeR092Yf26eNHaLvk3x3hRfP9y_Xh1Wy6_3dxdXS5LVwuWS7VqJGsaa1sK2JHaCg9ccFY55VsFlbINlZw7TqVcUacppRpapVgtPHWWXxQfZt1dHH_ufcqmD8n5rrODH_fJ0LqSADVV8v8o1xqEqIEi-v4fdDvuI14KqUoqUFpVGqmPM-XimFL0rdnF0Ns4GQrm4JVBr4xSqCwQfneU3De9X53QJ3MQuJkB_A3OduPQhcH_Hew2HOWmZBguaADQeYZBGiwFPhVjIDSHCpU-z0rblO3an0bZmIPr_NNW7LjaqX9w3PiB_wFLfbrG</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>Jung, Yi-Deun</creator><creator>Lee, Ja-Rang</creator><creator>Kim, Yun-Ji</creator><creator>Ha, Hong-Seok</creator><creator>Oh, Keon-Bong</creator><creator>Im, Gi-Sun</creator><creator>Choi, Bong-Hwan</creator><creator>Kim, Heui-Soo</creator><general>The Genetics Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>2013</creationdate><title>Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig</title><author>Jung, Yi-Deun ; Lee, Ja-Rang ; Kim, Yun-Ji ; Ha, Hong-Seok ; Oh, Keon-Bong ; Im, Gi-Sun ; Choi, Bong-Hwan ; Kim, Heui-Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c652t-8db72bbaaf10c6579a5e035324c8ef8048ab1733c3177d1c911190f88265e1ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>DNA Methylation</topic><topic>Endogenous Retroviruses - genetics</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>long terminal repeat (LTR)</topic><topic>methylation</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Porcine endogenous retrovirus</topic><topic>porcine endogenous retrovirus (PERV)</topic><topic>promoter activity</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Terminal Repeat Sequences - genetics</topic><topic>transcription factor-binding sites</topic><topic>Transcription, Genetic - genetics</topic><topic>United States</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, Yi-Deun</creatorcontrib><creatorcontrib>Lee, Ja-Rang</creatorcontrib><creatorcontrib>Kim, Yun-Ji</creatorcontrib><creatorcontrib>Ha, Hong-Seok</creatorcontrib><creatorcontrib>Oh, Keon-Bong</creatorcontrib><creatorcontrib>Im, Gi-Sun</creatorcontrib><creatorcontrib>Choi, Bong-Hwan</creatorcontrib><creatorcontrib>Kim, Heui-Soo</creatorcontrib><creatorcontrib>National Livestock Research Institute</creatorcontrib><creatorcontrib>Department of Biological Sciences</creatorcontrib><creatorcontrib>Pusan National University</creatorcontrib><creatorcontrib>Dankook University</creatorcontrib><creatorcontrib>Animal Genomics & Bioinformatics Division</creatorcontrib><creatorcontrib>RDA</creatorcontrib><creatorcontrib>College of Natural Sciences</creatorcontrib><creatorcontrib>National Institute of Animal Science</creatorcontrib><creatorcontrib>Department of Nanobiomedical Science & WCU Research Center</creatorcontrib><creatorcontrib>Animal Biotechnology Division</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & Genetic Systems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Yi-Deun</au><au>Lee, Ja-Rang</au><au>Kim, Yun-Ji</au><au>Ha, Hong-Seok</au><au>Oh, Keon-Bong</au><au>Im, Gi-Sun</au><au>Choi, Bong-Hwan</au><au>Kim, Heui-Soo</au><aucorp>National Livestock Research Institute</aucorp><aucorp>Department of Biological Sciences</aucorp><aucorp>Pusan National University</aucorp><aucorp>Dankook University</aucorp><aucorp>Animal Genomics & Bioinformatics Division</aucorp><aucorp>RDA</aucorp><aucorp>College of Natural Sciences</aucorp><aucorp>National Institute of Animal Science</aucorp><aucorp>Department of Nanobiomedical Science & WCU Research Center</aucorp><aucorp>Animal Biotechnology Division</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig</atitle><jtitle>Genes & Genetic Systems</jtitle><addtitle>Genes Genet. Syst.</addtitle><date>2013</date><risdate>2013</risdate><volume>88</volume><issue>2</issue><spage>135</spage><epage>142</epage><pages>135-142</pages><issn>1341-7568</issn><eissn>1880-5779</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promoter activities of 3 LTR structures (PERV-LTR1, LTR2, and LTR3 elements) belonging to the PERV-A family were examined using luciferase reporter genes in human liver cell lines (HepG2 and Hep3B). The PERV LTR3 element exhibited hypomethylation and stronger promoter activity than the other LTR elements in human liver cells. We also performed comparative sequences analysis of the PERV LTR elements by using bioinformatics tools. Our findings showed that several transcription factors such as Nkx2-2 and Elk-1 positively influenced the high transcriptional activity of the PERV LTR3 element.</abstract><cop>Japan</cop><pub>The Genetics Society of Japan</pub><pmid>23832305</pmid><doi>10.1266/ggs.88.135</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Base Sequence DNA Methylation Endogenous Retroviruses - genetics Hep G2 Cells Humans long terminal repeat (LTR) methylation Molecular Sequence Data Polymerase Chain Reaction Porcine endogenous retrovirus porcine endogenous retrovirus (PERV) promoter activity Promoter Regions, Genetic - genetics Sequence Homology, Nucleic Acid Swine Swine, Miniature Terminal Repeat Sequences - genetics transcription factor-binding sites Transcription, Genetic - genetics United States |
title | Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig |
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