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lncRNA H19/miR‐675 axis represses prostate cancer metastasis by targeting TGFBI
Prostate cancer is a leading cause of cancer‐related mortality in men worldwide and there is a lack of effective treatment options for advanced (metastatic) prostate cancer. Currently, limited knowledge is available concerning the role of long non‐coding RNAs in prostate cancer metastasis. In this s...
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| Published in: | The FEBS journal 2014-08, Vol.281 (16), p.3766-3775 |
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| Main Authors: | , , , , , , , , , , |
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| container_end_page | 3775 |
| container_issue | 16 |
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| container_title | The FEBS journal |
| container_volume | 281 |
| creator | Zhu, Miaojun Chen, Qin Liu, Xin Sun, Qian Zhao, Xian Deng, Rong Wang, Yanli Huang, Jian Xu, Ming Yan, Jianshe Yu, Jianxiu |
| description | Prostate cancer is a leading cause of cancer‐related mortality in men worldwide and there is a lack of effective treatment options for advanced (metastatic) prostate cancer. Currently, limited knowledge is available concerning the role of long non‐coding RNAs in prostate cancer metastasis. In this study, we found that long non‐coding RNA H19 (H19) and H19‐derived microRNA‐675 (miR‐675) were significantly downregulated in the metastatic prostate cancer cell line M12 compared with the non‐metastatic prostate epithelial cell line P69. Upregulation of H19 in P69 and PC3 cells significantly increased the level of miR‐675 and repressed cell migration; however, ectopic expression of H19 in M12 cells could not increase the level of miR‐675 and therefore had no effect on cell migration. Furthermore, we found that the expression level of either H19 or miR‐675 in P69 cells was negatively associated with the expression of transforming growth factor β induced protein (TGFBI), an extracellular matrix protein involved in cancer metastasis. Dual luciferase reporter assays showed that miR‐675 directly bound with 3′UTR of TGFBI mRNA to repress its translation. Taken together, we show for the first time that the H19–miR‐675 axis acts as a suppressor of prostate cancer metastasis, which may have possible diagnostic and therapeutic potential for advanced prostate cancer.
In this study, we show long non‐coding RNA H19 represses prostate cancer metastasis in a miR‐675‐dependent manner. We further identify miR‐675 directly inhibits the expression of an ECM protein TGFBI, which is involved in migration of cancer cells. Our findings provide a novel understanding of the roles of H19/miR‐675 in prostate cancer metastasis and the mechanisms involved. |
| doi_str_mv | 10.1111/febs.12902 |
| format | article |
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In this study, we show long non‐coding RNA H19 represses prostate cancer metastasis in a miR‐675‐dependent manner. We further identify miR‐675 directly inhibits the expression of an ECM protein TGFBI, which is involved in migration of cancer cells. Our findings provide a novel understanding of the roles of H19/miR‐675 in prostate cancer metastasis and the mechanisms involved.</description><identifier>ISSN: 1742-464X</identifier><identifier>EISSN: 1742-4658</identifier><identifier>DOI: 10.1111/febs.12902</identifier><identifier>PMID: 24988946</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>3' Untranslated Regions ; Base Sequence ; Binding Sites ; Bone Neoplasms - genetics ; Bone Neoplasms - metabolism ; Bone Neoplasms - secondary ; Cell adhesion & migration ; Cell Line, Tumor ; Cell Movement ; Epithelial Cells - physiology ; Extracellular Matrix Proteins - genetics ; Extracellular Matrix Proteins - metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; lncRNA H19 ; Male ; Medical research ; Metastasis ; MicroRNAs - genetics ; miR‐675 ; Prostate - pathology ; Prostate cancer ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Ribonucleic acid ; RNA ; RNA Interference ; RNA, Long Noncoding - genetics ; RNA, Messenger - genetics ; TGFBI ; Transforming Growth Factor beta - genetics ; Transforming Growth Factor beta - metabolism ; tumor metastasis</subject><ispartof>The FEBS journal, 2014-08, Vol.281 (16), p.3766-3775</ispartof><rights>2014 FEBS</rights><rights>2014 FEBS.</rights><rights>Copyright © 2014 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ffebs.12902$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ffebs.12902$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24988946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Miaojun</creatorcontrib><creatorcontrib>Chen, Qin</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Sun, Qian</creatorcontrib><creatorcontrib>Zhao, Xian</creatorcontrib><creatorcontrib>Deng, Rong</creatorcontrib><creatorcontrib>Wang, Yanli</creatorcontrib><creatorcontrib>Huang, Jian</creatorcontrib><creatorcontrib>Xu, Ming</creatorcontrib><creatorcontrib>Yan, Jianshe</creatorcontrib><creatorcontrib>Yu, Jianxiu</creatorcontrib><title>lncRNA H19/miR‐675 axis represses prostate cancer metastasis by targeting TGFBI</title><title>The FEBS journal</title><addtitle>FEBS J</addtitle><description>Prostate cancer is a leading cause of cancer‐related mortality in men worldwide and there is a lack of effective treatment options for advanced (metastatic) prostate cancer. Currently, limited knowledge is available concerning the role of long non‐coding RNAs in prostate cancer metastasis. In this study, we found that long non‐coding RNA H19 (H19) and H19‐derived microRNA‐675 (miR‐675) were significantly downregulated in the metastatic prostate cancer cell line M12 compared with the non‐metastatic prostate epithelial cell line P69. Upregulation of H19 in P69 and PC3 cells significantly increased the level of miR‐675 and repressed cell migration; however, ectopic expression of H19 in M12 cells could not increase the level of miR‐675 and therefore had no effect on cell migration. Furthermore, we found that the expression level of either H19 or miR‐675 in P69 cells was negatively associated with the expression of transforming growth factor β induced protein (TGFBI), an extracellular matrix protein involved in cancer metastasis. Dual luciferase reporter assays showed that miR‐675 directly bound with 3′UTR of TGFBI mRNA to repress its translation. Taken together, we show for the first time that the H19–miR‐675 axis acts as a suppressor of prostate cancer metastasis, which may have possible diagnostic and therapeutic potential for advanced prostate cancer.
In this study, we show long non‐coding RNA H19 represses prostate cancer metastasis in a miR‐675‐dependent manner. We further identify miR‐675 directly inhibits the expression of an ECM protein TGFBI, which is involved in migration of cancer cells. Our findings provide a novel understanding of the roles of H19/miR‐675 in prostate cancer metastasis and the mechanisms involved.</description><subject>3' Untranslated Regions</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Bone Neoplasms - genetics</subject><subject>Bone Neoplasms - metabolism</subject><subject>Bone Neoplasms - secondary</subject><subject>Cell adhesion & migration</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Epithelial Cells - physiology</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>lncRNA H19</subject><subject>Male</subject><subject>Medical research</subject><subject>Metastasis</subject><subject>MicroRNAs - genetics</subject><subject>miR‐675</subject><subject>Prostate - pathology</subject><subject>Prostate cancer</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>TGFBI</subject><subject>Transforming Growth Factor beta - genetics</subject><subject>Transforming Growth Factor beta - metabolism</subject><subject>tumor metastasis</subject><issn>1742-464X</issn><issn>1742-4658</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpdkU1OwzAQhS0EoqWw4QDIEhs2aWPHdpxlW_VPqkCUIrGznGRSpUrSYqeC7jgCZ-QkuD90wWzmaebT6GkeQrfEbxNXnQxi2yY08ukZapKQUY8JLs9Pmr010JW1S98POIuiS9SgLJIyYqKJnosqmT128ZhEnTKf_Xx9i5Bj_ZlbbGBtwFqweG1WttY14ERXCRhcQq3dwDoo3uJamwXUebXA89GwN7lGF5kuLNwcewu9Dgfz_tibPo0m_e7UWzIiqJelFNIs5qnUjGWpZEC40CxONM2EsxcmkuuQxkSHUlMiIWaBD1ozP8icEkELPRzuOnfvG7C1KnObQFHoClYbqwjnnLJQsNCh9__Q5WpjKuduRzHCCQkCR90dqU1cQqrWJi-12aq_ZzmAHICPvIDtaU98tYtB7WJQ-xjUcNB72avgF4pSeUw</recordid><startdate>201408</startdate><enddate>201408</enddate><creator>Zhu, Miaojun</creator><creator>Chen, Qin</creator><creator>Liu, Xin</creator><creator>Sun, Qian</creator><creator>Zhao, Xian</creator><creator>Deng, Rong</creator><creator>Wang, Yanli</creator><creator>Huang, Jian</creator><creator>Xu, Ming</creator><creator>Yan, Jianshe</creator><creator>Yu, Jianxiu</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201408</creationdate><title>lncRNA H19/miR‐675 axis represses prostate cancer metastasis by targeting TGFBI</title><author>Zhu, Miaojun ; 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Currently, limited knowledge is available concerning the role of long non‐coding RNAs in prostate cancer metastasis. In this study, we found that long non‐coding RNA H19 (H19) and H19‐derived microRNA‐675 (miR‐675) were significantly downregulated in the metastatic prostate cancer cell line M12 compared with the non‐metastatic prostate epithelial cell line P69. Upregulation of H19 in P69 and PC3 cells significantly increased the level of miR‐675 and repressed cell migration; however, ectopic expression of H19 in M12 cells could not increase the level of miR‐675 and therefore had no effect on cell migration. Furthermore, we found that the expression level of either H19 or miR‐675 in P69 cells was negatively associated with the expression of transforming growth factor β induced protein (TGFBI), an extracellular matrix protein involved in cancer metastasis. Dual luciferase reporter assays showed that miR‐675 directly bound with 3′UTR of TGFBI mRNA to repress its translation. Taken together, we show for the first time that the H19–miR‐675 axis acts as a suppressor of prostate cancer metastasis, which may have possible diagnostic and therapeutic potential for advanced prostate cancer.
In this study, we show long non‐coding RNA H19 represses prostate cancer metastasis in a miR‐675‐dependent manner. We further identify miR‐675 directly inhibits the expression of an ECM protein TGFBI, which is involved in migration of cancer cells. Our findings provide a novel understanding of the roles of H19/miR‐675 in prostate cancer metastasis and the mechanisms involved.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24988946</pmid><doi>10.1111/febs.12902</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3' Untranslated Regions Base Sequence Binding Sites Bone Neoplasms - genetics Bone Neoplasms - metabolism Bone Neoplasms - secondary Cell adhesion & migration Cell Line, Tumor Cell Movement Epithelial Cells - physiology Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - metabolism Gene Expression Regulation, Neoplastic Humans lncRNA H19 Male Medical research Metastasis MicroRNAs - genetics miR‐675 Prostate - pathology Prostate cancer Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Ribonucleic acid RNA RNA Interference RNA, Long Noncoding - genetics RNA, Messenger - genetics TGFBI Transforming Growth Factor beta - genetics Transforming Growth Factor beta - metabolism tumor metastasis |
| title | lncRNA H19/miR‐675 axis represses prostate cancer metastasis by targeting TGFBI |
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