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Mutational Analysis of the Virion-sense Genes of Maize Streak Virus
1 John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K. 2 Institute of Applied Microbiology, University of Agriculture and Forestry, Peter Jordanstrasse 82, A1190 Vienna, Austria and 3 Department of Plant Pathology, University of California, Riverside, Ca...
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| Published in: | Journal of general virology 1989-09, Vol.70 (9), p.2309-2323 |
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| container_end_page | 2323 |
| container_issue | 9 |
| container_start_page | 2309 |
| container_title | Journal of general virology |
| container_volume | 70 |
| creator | Boulton, Margaret I Steinkellner, Herta Donson, Jonathan Markham, Peter G King, Donna I Davies, Jeffrey W |
| description | 1 John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K.
2 Institute of Applied Microbiology, University of Agriculture and Forestry, Peter Jordanstrasse 82, A1190 Vienna, Austria
and 3 Department of Plant Pathology, University of California, Riverside, California 92521, U.S.A.
Insertion and deletion mutagenesis of the two virion-sense genes, V1 and V2, of maize streak virus (MSV) prevents symptomatic infections following Agrobacterium -mediated agroinoculation of maize seedlings. These genes code for an M r 10900 protein and for coat protein, respectively. Mutants containing insertions or deletions in the coat protein gene, V2, were able to replicate to low levels, producing dsDNA although virion ssDNA was not detected and symptoms were not observed. Hence, unlike the bipartite geminiviruses, MSV requires coat protein to produce symptomatic systemic infection. Mutations in gene V1 which considerably shortened the M r 10900 protein (V1 gene) resulted either in low levels of replication, in which all the DNA forms associated with a wild-type infection were produced, or in no infection, in which case coat protein production may also have been affected. A V1 mutant generated in vivo with 11 of the 14 N-terminal amino acids altered, was viable and produced symptoms typical of a wild-type infection. Infectivity, assessed by replication and symptom expression, was restored by co-inoculating constructs containing single mutations in different open reading frames, thus rescue can occur by trans-complementation of gene products. The experiments showed that the mutations did not affect the nucleotide sequence requirements for replication and that in all cases intermolecular recombination eventually resulted in dominant wild-type virus.
Keywords: MSV, mutagenesis, complementation, recombination
Received 6 March 1989;
accepted 26 April 1989. |
| doi_str_mv | 10.1099/0022-1317-70-9-2309 |
| format | article |
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2 Institute of Applied Microbiology, University of Agriculture and Forestry, Peter Jordanstrasse 82, A1190 Vienna, Austria
and 3 Department of Plant Pathology, University of California, Riverside, California 92521, U.S.A.
Insertion and deletion mutagenesis of the two virion-sense genes, V1 and V2, of maize streak virus (MSV) prevents symptomatic infections following Agrobacterium -mediated agroinoculation of maize seedlings. These genes code for an M r 10900 protein and for coat protein, respectively. Mutants containing insertions or deletions in the coat protein gene, V2, were able to replicate to low levels, producing dsDNA although virion ssDNA was not detected and symptoms were not observed. Hence, unlike the bipartite geminiviruses, MSV requires coat protein to produce symptomatic systemic infection. Mutations in gene V1 which considerably shortened the M r 10900 protein (V1 gene) resulted either in low levels of replication, in which all the DNA forms associated with a wild-type infection were produced, or in no infection, in which case coat protein production may also have been affected. A V1 mutant generated in vivo with 11 of the 14 N-terminal amino acids altered, was viable and produced symptoms typical of a wild-type infection. Infectivity, assessed by replication and symptom expression, was restored by co-inoculating constructs containing single mutations in different open reading frames, thus rescue can occur by trans-complementation of gene products. The experiments showed that the mutations did not affect the nucleotide sequence requirements for replication and that in all cases intermolecular recombination eventually resulted in dominant wild-type virus.
Keywords: MSV, mutagenesis, complementation, recombination
Received 6 March 1989;
accepted 26 April 1989.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-70-9-2309</identifier><identifier>PMID: 2550570</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Base Sequence ; Capsid - genetics ; Chromosome Deletion ; DNA Transposable Elements ; Genes ; Genes, Viral ; Molecular Sequence Data ; Mutation ; Plant Viruses - genetics ; Plasmids ; Rhizobium - genetics ; Virion - genetics</subject><ispartof>Journal of general virology, 1989-09, Vol.70 (9), p.2309-2323</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-1c3f72ec42caae94ddf697351e7f5d46ba6dcfc606665bb23cca41e5a9be4ef03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2550570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boulton, Margaret I</creatorcontrib><creatorcontrib>Steinkellner, Herta</creatorcontrib><creatorcontrib>Donson, Jonathan</creatorcontrib><creatorcontrib>Markham, Peter G</creatorcontrib><creatorcontrib>King, Donna I</creatorcontrib><creatorcontrib>Davies, Jeffrey W</creatorcontrib><title>Mutational Analysis of the Virion-sense Genes of Maize Streak Virus</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K.
2 Institute of Applied Microbiology, University of Agriculture and Forestry, Peter Jordanstrasse 82, A1190 Vienna, Austria
and 3 Department of Plant Pathology, University of California, Riverside, California 92521, U.S.A.
Insertion and deletion mutagenesis of the two virion-sense genes, V1 and V2, of maize streak virus (MSV) prevents symptomatic infections following Agrobacterium -mediated agroinoculation of maize seedlings. These genes code for an M r 10900 protein and for coat protein, respectively. Mutants containing insertions or deletions in the coat protein gene, V2, were able to replicate to low levels, producing dsDNA although virion ssDNA was not detected and symptoms were not observed. Hence, unlike the bipartite geminiviruses, MSV requires coat protein to produce symptomatic systemic infection. Mutations in gene V1 which considerably shortened the M r 10900 protein (V1 gene) resulted either in low levels of replication, in which all the DNA forms associated with a wild-type infection were produced, or in no infection, in which case coat protein production may also have been affected. A V1 mutant generated in vivo with 11 of the 14 N-terminal amino acids altered, was viable and produced symptoms typical of a wild-type infection. Infectivity, assessed by replication and symptom expression, was restored by co-inoculating constructs containing single mutations in different open reading frames, thus rescue can occur by trans-complementation of gene products. The experiments showed that the mutations did not affect the nucleotide sequence requirements for replication and that in all cases intermolecular recombination eventually resulted in dominant wild-type virus.
Keywords: MSV, mutagenesis, complementation, recombination
Received 6 March 1989;
accepted 26 April 1989.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Capsid - genetics</subject><subject>Chromosome Deletion</subject><subject>DNA Transposable Elements</subject><subject>Genes</subject><subject>Genes, Viral</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plant Viruses - genetics</subject><subject>Plasmids</subject><subject>Rhizobium - genetics</subject><subject>Virion - genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNpFkMtOwzAQRS0EKqXwBQgpK8TGYDu2g5dVBQWpFQseW8txxq0hTYqdgMrXk9CqbGYW584d6SB0Tsk1JUrdEMIYpinNcEawwiwl6gANKZcCs44fouE-cYxOYnwnhHIusgEaMCGIyMgQTeZtYxpfV6ZMxt3YRB-T2iXNEpI3HzqAI1QRkilU8Efmxv9A8twEMB99pI2n6MiZMsLZbo_Q6_3dy-QBz56mj5PxDFtOVIOpTV3GwHJmjQHFi8JJlaWCQuZEwWVuZGGdlURKKfKcpdYaTkEYlQMHR9IRutz2rkP92UJs9MpHC2VpKqjbqKngXPLbPphugzbUMQZweh38yoSNpkT36nQvRvdidEa00r267upiV9_mKyj2NztXHb_a8qVfLL99AL2AauW7H7mv9ZcP_1W_XM93yQ</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Boulton, Margaret I</creator><creator>Steinkellner, Herta</creator><creator>Donson, Jonathan</creator><creator>Markham, Peter G</creator><creator>King, Donna I</creator><creator>Davies, Jeffrey W</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19890901</creationdate><title>Mutational Analysis of the Virion-sense Genes of Maize Streak Virus</title><author>Boulton, Margaret I ; Steinkellner, Herta ; Donson, Jonathan ; Markham, Peter G ; King, Donna I ; Davies, Jeffrey W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-1c3f72ec42caae94ddf697351e7f5d46ba6dcfc606665bb23cca41e5a9be4ef03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Capsid - genetics</topic><topic>Chromosome Deletion</topic><topic>DNA Transposable Elements</topic><topic>Genes</topic><topic>Genes, Viral</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plant Viruses - genetics</topic><topic>Plasmids</topic><topic>Rhizobium - genetics</topic><topic>Virion - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boulton, Margaret I</creatorcontrib><creatorcontrib>Steinkellner, Herta</creatorcontrib><creatorcontrib>Donson, Jonathan</creatorcontrib><creatorcontrib>Markham, Peter G</creatorcontrib><creatorcontrib>King, Donna I</creatorcontrib><creatorcontrib>Davies, Jeffrey W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boulton, Margaret I</au><au>Steinkellner, Herta</au><au>Donson, Jonathan</au><au>Markham, Peter G</au><au>King, Donna I</au><au>Davies, Jeffrey W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutational Analysis of the Virion-sense Genes of Maize Streak Virus</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>70</volume><issue>9</issue><spage>2309</spage><epage>2323</epage><pages>2309-2323</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><abstract>1 John Innes Institute, AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K.
2 Institute of Applied Microbiology, University of Agriculture and Forestry, Peter Jordanstrasse 82, A1190 Vienna, Austria
and 3 Department of Plant Pathology, University of California, Riverside, California 92521, U.S.A.
Insertion and deletion mutagenesis of the two virion-sense genes, V1 and V2, of maize streak virus (MSV) prevents symptomatic infections following Agrobacterium -mediated agroinoculation of maize seedlings. These genes code for an M r 10900 protein and for coat protein, respectively. Mutants containing insertions or deletions in the coat protein gene, V2, were able to replicate to low levels, producing dsDNA although virion ssDNA was not detected and symptoms were not observed. Hence, unlike the bipartite geminiviruses, MSV requires coat protein to produce symptomatic systemic infection. Mutations in gene V1 which considerably shortened the M r 10900 protein (V1 gene) resulted either in low levels of replication, in which all the DNA forms associated with a wild-type infection were produced, or in no infection, in which case coat protein production may also have been affected. A V1 mutant generated in vivo with 11 of the 14 N-terminal amino acids altered, was viable and produced symptoms typical of a wild-type infection. Infectivity, assessed by replication and symptom expression, was restored by co-inoculating constructs containing single mutations in different open reading frames, thus rescue can occur by trans-complementation of gene products. The experiments showed that the mutations did not affect the nucleotide sequence requirements for replication and that in all cases intermolecular recombination eventually resulted in dominant wild-type virus.
Keywords: MSV, mutagenesis, complementation, recombination
Received 6 March 1989;
accepted 26 April 1989.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>2550570</pmid><doi>10.1099/0022-1317-70-9-2309</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1989-09, Vol.70 (9), p.2309-2323 |
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| language | eng |
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| source | Freely Accessible Science Journals - check A-Z of ejournals |
| subjects | Amino Acid Sequence Base Sequence Capsid - genetics Chromosome Deletion DNA Transposable Elements Genes Genes, Viral Molecular Sequence Data Mutation Plant Viruses - genetics Plasmids Rhizobium - genetics Virion - genetics |
| title | Mutational Analysis of the Virion-sense Genes of Maize Streak Virus |
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