Active removal of inorganic phosphate from cerebrospinal fluid by the choroid plexus

The P(i) concentration of mammalian cerebrospinal fluid (CSF) is about one-half that of plasma, a phenomenon also shown here in the spiny dogfish, Squalus acanthias. The objective of the present study was to characterize the possible role of the choroid plexus (CP) in determining CSF P(i) concentrat...

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Published in:American journal of physiology. Renal physiology 2014-06, Vol.306 (11), p.F1275-F1284
Main Authors: Guerreiro, Pedro M, Bataille, Amy M, Parker, Sonda L, Renfro, J Larry
Format: Article
Language:English
Subjects:
Absorption
Animals
Biological Transport - physiology
Biological Transport, Active
Body fluids
Cerebrospinal Fluid - metabolism
Choroid Plexus - metabolism
Dogfish
Female
Gene expression
Immunohistochemistry
Male
Membranes
Neurochemistry
Phosphates
Phosphates - metabolism
Rats
Sharks
Sodium-Phosphate Cotransporter Proteins, Type III - metabolism
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container_issue 11
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container_title American journal of physiology. Renal physiology
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creator Guerreiro, Pedro M
Bataille, Amy M
Parker, Sonda L
Renfro, J Larry
description The P(i) concentration of mammalian cerebrospinal fluid (CSF) is about one-half that of plasma, a phenomenon also shown here in the spiny dogfish, Squalus acanthias. The objective of the present study was to characterize the possible role of the choroid plexus (CP) in determining CSF P(i) concentration. The large sheet-like fourth CP of the shark was mounted in Ussing chambers where unidirectional (33)P(i) fluxes revealed potent active transport from CSF to the blood side under short-circuited conditions. The flux ratio was 8:1 with an average transepithelial resistance of 87 ± 17.9 Ω·cm(2) and electrical potential difference of +0.9 ± 0.17 mV (CSF side positive). Active P(i) absorption from CSF was inhibited by 10 mM arsenate, 0.2 mM ouabain, Na(+)-free medium, and increasing the K(+) concentration from 5 to 100 mM. Li(+) stimulated transport twofold compared with Na(+)-free medium. Phosphonoformic acid (1 mM) had no effect on active P(i) transport. RT-PCR revealed both P(i) transporter (PiT)1 and PiT2 (SLC20 family) gene expression, but no Na(+)-P(i) cotransporter II (SLC34 family) expression, in the shark CP. PiT2 immunoreactivity was shown by immunoblot analysis and localized by immunohistochemistry in (or near) the CP apical microvillar membranes of both the shark and rat. PiT1 appeared to be localized primarily to vascular endothelial cells. Taken together, these data indicate that the CP actively removes P(i) from CSF. This process has transport properties consistent with a PiT2, Na(+)-dependent transporter that is located in the apical region of the CP epithelium.
doi_str_mv 10.1152/ajprenal.00458.2013
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The objective of the present study was to characterize the possible role of the choroid plexus (CP) in determining CSF P(i) concentration. The large sheet-like fourth CP of the shark was mounted in Ussing chambers where unidirectional (33)P(i) fluxes revealed potent active transport from CSF to the blood side under short-circuited conditions. The flux ratio was 8:1 with an average transepithelial resistance of 87 ± 17.9 Ω·cm(2) and electrical potential difference of +0.9 ± 0.17 mV (CSF side positive). Active P(i) absorption from CSF was inhibited by 10 mM arsenate, 0.2 mM ouabain, Na(+)-free medium, and increasing the K(+) concentration from 5 to 100 mM. Li(+) stimulated transport twofold compared with Na(+)-free medium. Phosphonoformic acid (1 mM) had no effect on active P(i) transport. RT-PCR revealed both P(i) transporter (PiT)1 and PiT2 (SLC20 family) gene expression, but no Na(+)-P(i) cotransporter II (SLC34 family) expression, in the shark CP. 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Renal physiology</title><addtitle>Am J Physiol Renal Physiol</addtitle><description>The P(i) concentration of mammalian cerebrospinal fluid (CSF) is about one-half that of plasma, a phenomenon also shown here in the spiny dogfish, Squalus acanthias. The objective of the present study was to characterize the possible role of the choroid plexus (CP) in determining CSF P(i) concentration. The large sheet-like fourth CP of the shark was mounted in Ussing chambers where unidirectional (33)P(i) fluxes revealed potent active transport from CSF to the blood side under short-circuited conditions. The flux ratio was 8:1 with an average transepithelial resistance of 87 ± 17.9 Ω·cm(2) and electrical potential difference of +0.9 ± 0.17 mV (CSF side positive). Active P(i) absorption from CSF was inhibited by 10 mM arsenate, 0.2 mM ouabain, Na(+)-free medium, and increasing the K(+) concentration from 5 to 100 mM. Li(+) stimulated transport twofold compared with Na(+)-free medium. 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ispartof American journal of physiology. Renal physiology, 2014-06, Vol.306 (11), p.F1275-F1284
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subjects Absorption
Animals
Biological Transport - physiology
Biological Transport, Active
Body fluids
Cerebrospinal Fluid - metabolism
Choroid Plexus - metabolism
Dogfish
Female
Gene expression
Immunohistochemistry
Male
Membranes
Neurochemistry
Phosphates
Phosphates - metabolism
Rats
Sharks
Sodium-Phosphate Cotransporter Proteins, Type III - metabolism
title Active removal of inorganic phosphate from cerebrospinal fluid by the choroid plexus
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