A Novel Reassortant H2N3 Influenza Virus Isolated from China

Objective To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market （LPM） in 2009 in Guangdong province of China. Methods PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All seg...

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Bibliographic Details
Published in:Biomedical and environmental sciences 2014-04, Vol.27 (4), p.240-249
Main Authors: LI, Xiao Dan, ZOU, Shu Mei, ZHANG, Ye, BAI, Tian, GAO, Rong Bao, ZHANG, Xin, WU, Jie, SHU, Yue Long
Format: Article
Language:English
Subjects:
Animals
Avian influenza
China
Ducks - virology
Gene analysis
Genome, Viral
H2N3
Influenza A virus - genetics
Influenza A virus - isolation & purification
Influenza in Birds - virology
NA基因
Phylogeny
RT-PCR检测
中国
家禽市场
病毒分离株
禽流感病毒
系统进化树
遗传组成
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Summary:Objective To analyze the genetic composition of a novel H2N3 virus isolate identified from a duck cage swab in a live poultry market （LPM） in 2009 in Guangdong province of China. Methods PCR-positive specimens were inoculated into embryonated chicken eggs and subtyped by conventional RT-PCR. All segments of the virus A/environment/Guangdong/2/2009 were sequenced, and phylogenetic trees were constructed and analyzed. Results The genes of this virus belong to Eurasian-lineage avian viruses. The virus is a reassortant with the HA gene from an H2N2 virus and the NA gene from an H5N3 virus. The PB1, PB2, and NP genes were from an H4N6 virus, the PA was from an H3N8 virus, the M gene was from an H1N3 virus, and the NS gene was from an H10N6 virus. Conclusion market. Its A novel avian-origin reassortant H2N3 influenza virus was detected in a live poultry potential impacts and evolution should be closely monitored.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2014.047