Progress on the development of rapid methods for antimicrobial susceptibility testing

Antimicrobial susceptibility testing is essential for guiding the treatment of many types of bacterial infections, especially in the current context of rising rates of antibiotic resistance. The most commonly employed methods rely on the detection of phenotypic resistance by measuring bacterial grow...

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Published in:Journal of antimicrobial chemotherapy 2013-12, Vol.68 (12), p.2710-2717
Main Authors: Pulido, Marina R, García-Quintanilla, Meritxell, Martín-Peña, Reyes, Cisneros, José Miguel, McConnell, Michael J
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteria - drug effects
Bacterial infections
Bacterial Infections - drug therapy
Bacterial Infections - microbiology
Drug resistance
Genotype & phenotype
Humans
Mass spectrometry
Microbial Sensitivity Tests - methods
Microbial Sensitivity Tests - trends
Molecular Diagnostic Techniques - methods
Molecular Diagnostic Techniques - trends
Polymerase chain reaction
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cited_by cdi_FETCH-LOGICAL-c384t-ca649fb13eb035dbbc763be93f8f43ea9e1e8bdf5595c7651aae1f3230deac563
cites cdi_FETCH-LOGICAL-c384t-ca649fb13eb035dbbc763be93f8f43ea9e1e8bdf5595c7651aae1f3230deac563
container_end_page 2717
container_issue 12
container_start_page 2710
container_title Journal of antimicrobial chemotherapy
container_volume 68
creator Pulido, Marina R
García-Quintanilla, Meritxell
Martín-Peña, Reyes
Cisneros, José Miguel
McConnell, Michael J
description Antimicrobial susceptibility testing is essential for guiding the treatment of many types of bacterial infections, especially in the current context of rising rates of antibiotic resistance. The most commonly employed methods rely on the detection of phenotypic resistance by measuring bacterial growth in the presence of the antibiotic being tested. Although these methods are highly sensitive for the detection of resistance, they require that the bacterial pathogen is isolated from the clinical sample before testing and must employ incubation times that are sufficient for differentiating resistant from susceptible isolates. Knowledge regarding the molecular determinants of antibiotic resistance has facilitated the development of novel approaches for the rapid detection of resistance in bacterial pathogens. PCR-based techniques, mass spectrometry, microarrays, microfluidics, cell lysis-based approaches and whole-genome sequencing have all demonstrated the ability to detect resistance in various bacterial species. However, it remains to be determined whether these methods can achieve sufficient sensitivity and specificity compared with standard phenotypic resistance testing to justify their use in routine clinical practice. In the present review, we discuss recent progress in the development of methods for rapid antimicrobial susceptibility testing and highlight the limitations of each approach that still remain be addressed.
doi_str_mv 10.1093/jac/dkt253
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source Oxford Journals Online
subjects Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteria - drug effects
Bacterial infections
Bacterial Infections - drug therapy
Bacterial Infections - microbiology
Drug resistance
Genotype & phenotype
Humans
Mass spectrometry
Microbial Sensitivity Tests - methods
Microbial Sensitivity Tests - trends
Molecular Diagnostic Techniques - methods
Molecular Diagnostic Techniques - trends
Polymerase chain reaction
title Progress on the development of rapid methods for antimicrobial susceptibility testing
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