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The antimicrobial effects of helium and helium–air plasma on Staphylococcus aureus and Clostridium difficile
Healthcare‐associated infections (HCAI) affect 5–10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approa...
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| Published in: | Letters in applied microbiology 2013-08, Vol.57 (2), p.83-90 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4511-1aa0db1886122734e096d4756500f463d137de301c01feae49640f0149acb2153 |
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| cites | cdi_FETCH-LOGICAL-c4511-1aa0db1886122734e096d4756500f463d137de301c01feae49640f0149acb2153 |
| container_end_page | 90 |
| container_issue | 2 |
| container_start_page | 83 |
| container_title | Letters in applied microbiology |
| container_volume | 57 |
| creator | Galvin, S. Cahill, O. O'Connor, N. Cafolla, A.A. Daniels, S. Humphreys, H. |
| description | Healthcare‐associated infections (HCAI) affect 5–10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such as cleaning with detergents and the use of chemical disinfectant are suboptimal. We assessed the efficacy of helium and helium–air plasma in killing Staphylococcus aureus and Clostridium difficile on a glass surface and studied the impact on bacterial cells using atomic force microscopy (AFM). Both plasma types exhibited bactericidal effects on Staph. aureus (log3·6 – >log7), with increased activity against methicillin‐resistant strains, but had a negligible effect on Cl. difficile spores ( |
| doi_str_mv | 10.1111/lam.12091 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1419372651</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3020982031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4511-1aa0db1886122734e096d4756500f463d137de301c01feae49640f0149acb2153</originalsourceid><addsrcrecordid>eNqN0VGL1DAQAOAgireePvgHpCDC-dC7mSRN2sdj8VRY8cETfCuzacLmSNs1aZF98z_4D_0lpu6qIAjmZQL5MsPMMPYU4RLzuQrUXyKHBu-xFUrNS62qT_fZCrhSZc0recYepXQHADXy5iE740KBrhWs2HC7swUNk--9iePWUyisc9ZMqRhdsbPBz31-707X71-_kY_FPlDqqRiH4sNE-90hjGY0Zk4FzdEuIX9YhzFN0XdLgs47540P9jF74Cgk--QUz9nHm1e36zfl5v3rt-vrTWlkhVgiEXRbrGuFnGshLTSqk7pSFYCTSnQodGcFoAF0lqxslAQHKBsyW46VOGcXx7z7OH6ebZra3idjQ6DBjnNqUWIjNFcV_gcFhIZrJTN9_he9G-c45EYWBRI4Is_q5VHlgaYUrWv30fcUDy1Cu-yrzftqf-4r22enjPO2t91v-WtBGbw4AUqGgos0GJ_-OF1xIfjS8NXRfclTPvy7Yru5fncs_QPGJ6tF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1400402112</pqid></control><display><type>article</type><title>The antimicrobial effects of helium and helium–air plasma on Staphylococcus aureus and Clostridium difficile</title><source>Wiley-Blackwell Journals</source><source>Oxford University Press Journals</source><creator>Galvin, S. ; Cahill, O. ; O'Connor, N. ; Cafolla, A.A. ; Daniels, S. ; Humphreys, H.</creator><creatorcontrib>Galvin, S. ; Cahill, O. ; O'Connor, N. ; Cafolla, A.A. ; Daniels, S. ; Humphreys, H.</creatorcontrib><description>Healthcare‐associated infections (HCAI) affect 5–10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such as cleaning with detergents and the use of chemical disinfectant are suboptimal. We assessed the efficacy of helium and helium–air plasma in killing Staphylococcus aureus and Clostridium difficile on a glass surface and studied the impact on bacterial cells using atomic force microscopy (AFM). Both plasma types exhibited bactericidal effects on Staph. aureus (log3·6 – >log7), with increased activity against methicillin‐resistant strains, but had a negligible effect on Cl. difficile spores (<1log). AFM demonstrated cell surface disruption. The addition of air increased the microbicidal activity of the plasma and decreased the exposure time required for an equivalent log reduction. Further evaluation of cold plasma systems is warranted with, for example, different bacteria and on surfaces more reminiscent of the health care environment as this approach has potential as an effective decontaminant.
Significance and Impact of the Study
Many bacterial causes of healthcare infection can survive in the inanimate environment for lengthy periods and be transmitted to patients. Furthermore, current methods of environmental decontamination such as detergents, chemical disinfectants or gaseous fumigation are suboptimal for a variety of reasons. We assessed the efficacy of helium and helium–air plasma as a decontaminant and demonstrated a significant reduction in bacterial counts of Staphylococcus aureus on a glass surface. Atomic force microscopy morphologically confirmed the impact on bacterial cells. This approach warrants further study as an alternative to current options for hospital hygiene.
Significance and Impact of the Study: Many bacterial causes of healthcare infection can survive in the inanimate environment for lengthy periods and be transmitted to patients. Furthermore, current methods of environmental decontamination such as detergents, chemical disinfectants or gaseous fumigation are suboptimal for a variety of reasons. We assessed the efficacy of helium and helium–air plasma as a decontaminant and demonstrated a significant reduction in bacterial counts of Staphylococcus aureus on a glass surface. Atomic force microscopy morphologically confirmed the impact on bacterial cells. This approach warrants further study as an alternative to current options for hospital hygiene.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/lam.12091</identifier><identifier>PMID: 23607860</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Air ; Bacterial Load ; biocides ; Biological and medical sciences ; Clostridium difficile ; Clostridium difficile - drug effects ; Clostridium difficile - physiology ; Clostridium difficile - ultrastructure ; Decontamination - methods ; Disinfectants - pharmacology ; disinfection ; environmental ; Fundamental and applied biological sciences. Psychology ; Glass ; Helium - pharmacology ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - physiology ; Methicillin-Resistant Staphylococcus aureus - ultrastructure ; microbial contamination ; Microbial Viability ; Microbiology ; Microscopy, Atomic Force ; Plasma Gases - pharmacology ; Spores, Bacterial - drug effects ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Staphylococcus aureus - ultrastructure</subject><ispartof>Letters in applied microbiology, 2013-08, Vol.57 (2), p.83-90</ispartof><rights>2013 The Society for Applied Microbiology</rights><rights>2014 INIST-CNRS</rights><rights>2013 The Society for Applied Microbiology.</rights><rights>Copyright © 2013 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4511-1aa0db1886122734e096d4756500f463d137de301c01feae49640f0149acb2153</citedby><cites>FETCH-LOGICAL-c4511-1aa0db1886122734e096d4756500f463d137de301c01feae49640f0149acb2153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Flam.12091$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Flam.12091$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27523325$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23607860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Galvin, S.</creatorcontrib><creatorcontrib>Cahill, O.</creatorcontrib><creatorcontrib>O'Connor, N.</creatorcontrib><creatorcontrib>Cafolla, A.A.</creatorcontrib><creatorcontrib>Daniels, S.</creatorcontrib><creatorcontrib>Humphreys, H.</creatorcontrib><title>The antimicrobial effects of helium and helium–air plasma on Staphylococcus aureus and Clostridium difficile</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Healthcare‐associated infections (HCAI) affect 5–10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such as cleaning with detergents and the use of chemical disinfectant are suboptimal. We assessed the efficacy of helium and helium–air plasma in killing Staphylococcus aureus and Clostridium difficile on a glass surface and studied the impact on bacterial cells using atomic force microscopy (AFM). Both plasma types exhibited bactericidal effects on Staph. aureus (log3·6 – >log7), with increased activity against methicillin‐resistant strains, but had a negligible effect on Cl. difficile spores (<1log). AFM demonstrated cell surface disruption. The addition of air increased the microbicidal activity of the plasma and decreased the exposure time required for an equivalent log reduction. Further evaluation of cold plasma systems is warranted with, for example, different bacteria and on surfaces more reminiscent of the health care environment as this approach has potential as an effective decontaminant.
Significance and Impact of the Study
Many bacterial causes of healthcare infection can survive in the inanimate environment for lengthy periods and be transmitted to patients. Furthermore, current methods of environmental decontamination such as detergents, chemical disinfectants or gaseous fumigation are suboptimal for a variety of reasons. We assessed the efficacy of helium and helium–air plasma as a decontaminant and demonstrated a significant reduction in bacterial counts of Staphylococcus aureus on a glass surface. Atomic force microscopy morphologically confirmed the impact on bacterial cells. This approach warrants further study as an alternative to current options for hospital hygiene.
Significance and Impact of the Study: Many bacterial causes of healthcare infection can survive in the inanimate environment for lengthy periods and be transmitted to patients. Furthermore, current methods of environmental decontamination such as detergents, chemical disinfectants or gaseous fumigation are suboptimal for a variety of reasons. We assessed the efficacy of helium and helium–air plasma as a decontaminant and demonstrated a significant reduction in bacterial counts of Staphylococcus aureus on a glass surface. Atomic force microscopy morphologically confirmed the impact on bacterial cells. This approach warrants further study as an alternative to current options for hospital hygiene.</description><subject>Air</subject><subject>Bacterial Load</subject><subject>biocides</subject><subject>Biological and medical sciences</subject><subject>Clostridium difficile</subject><subject>Clostridium difficile - drug effects</subject><subject>Clostridium difficile - physiology</subject><subject>Clostridium difficile - ultrastructure</subject><subject>Decontamination - methods</subject><subject>Disinfectants - pharmacology</subject><subject>disinfection</subject><subject>environmental</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glass</subject><subject>Helium - pharmacology</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - physiology</subject><subject>Methicillin-Resistant Staphylococcus aureus - ultrastructure</subject><subject>microbial contamination</subject><subject>Microbial Viability</subject><subject>Microbiology</subject><subject>Microscopy, Atomic Force</subject><subject>Plasma Gases - pharmacology</subject><subject>Spores, Bacterial - drug effects</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Staphylococcus aureus - ultrastructure</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqN0VGL1DAQAOAgireePvgHpCDC-dC7mSRN2sdj8VRY8cETfCuzacLmSNs1aZF98z_4D_0lpu6qIAjmZQL5MsPMMPYU4RLzuQrUXyKHBu-xFUrNS62qT_fZCrhSZc0recYepXQHADXy5iE740KBrhWs2HC7swUNk--9iePWUyisc9ZMqRhdsbPBz31-707X71-_kY_FPlDqqRiH4sNE-90hjGY0Zk4FzdEuIX9YhzFN0XdLgs47540P9jF74Cgk--QUz9nHm1e36zfl5v3rt-vrTWlkhVgiEXRbrGuFnGshLTSqk7pSFYCTSnQodGcFoAF0lqxslAQHKBsyW46VOGcXx7z7OH6ebZra3idjQ6DBjnNqUWIjNFcV_gcFhIZrJTN9_he9G-c45EYWBRI4Is_q5VHlgaYUrWv30fcUDy1Cu-yrzftqf-4r22enjPO2t91v-WtBGbw4AUqGgos0GJ_-OF1xIfjS8NXRfclTPvy7Yru5fncs_QPGJ6tF</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Galvin, S.</creator><creator>Cahill, O.</creator><creator>O'Connor, N.</creator><creator>Cafolla, A.A.</creator><creator>Daniels, S.</creator><creator>Humphreys, H.</creator><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201308</creationdate><title>The antimicrobial effects of helium and helium–air plasma on Staphylococcus aureus and Clostridium difficile</title><author>Galvin, S. ; Cahill, O. ; O'Connor, N. ; Cafolla, A.A. ; Daniels, S. ; Humphreys, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4511-1aa0db1886122734e096d4756500f463d137de301c01feae49640f0149acb2153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Air</topic><topic>Bacterial Load</topic><topic>biocides</topic><topic>Biological and medical sciences</topic><topic>Clostridium difficile</topic><topic>Clostridium difficile - drug effects</topic><topic>Clostridium difficile - physiology</topic><topic>Clostridium difficile - ultrastructure</topic><topic>Decontamination - methods</topic><topic>Disinfectants - pharmacology</topic><topic>disinfection</topic><topic>environmental</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glass</topic><topic>Helium - pharmacology</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - physiology</topic><topic>Methicillin-Resistant Staphylococcus aureus - ultrastructure</topic><topic>microbial contamination</topic><topic>Microbial Viability</topic><topic>Microbiology</topic><topic>Microscopy, Atomic Force</topic><topic>Plasma Gases - pharmacology</topic><topic>Spores, Bacterial - drug effects</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - physiology</topic><topic>Staphylococcus aureus - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galvin, S.</creatorcontrib><creatorcontrib>Cahill, O.</creatorcontrib><creatorcontrib>O'Connor, N.</creatorcontrib><creatorcontrib>Cafolla, A.A.</creatorcontrib><creatorcontrib>Daniels, S.</creatorcontrib><creatorcontrib>Humphreys, H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galvin, S.</au><au>Cahill, O.</au><au>O'Connor, N.</au><au>Cafolla, A.A.</au><au>Daniels, S.</au><au>Humphreys, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The antimicrobial effects of helium and helium–air plasma on Staphylococcus aureus and Clostridium difficile</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2013-08</date><risdate>2013</risdate><volume>57</volume><issue>2</issue><spage>83</spage><epage>90</epage><pages>83-90</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Article-2</notes><notes>ObjectType-Feature-1</notes><abstract>Healthcare‐associated infections (HCAI) affect 5–10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such as cleaning with detergents and the use of chemical disinfectant are suboptimal. We assessed the efficacy of helium and helium–air plasma in killing Staphylococcus aureus and Clostridium difficile on a glass surface and studied the impact on bacterial cells using atomic force microscopy (AFM). Both plasma types exhibited bactericidal effects on Staph. aureus (log3·6 – >log7), with increased activity against methicillin‐resistant strains, but had a negligible effect on Cl. difficile spores (<1log). AFM demonstrated cell surface disruption. The addition of air increased the microbicidal activity of the plasma and decreased the exposure time required for an equivalent log reduction. Further evaluation of cold plasma systems is warranted with, for example, different bacteria and on surfaces more reminiscent of the health care environment as this approach has potential as an effective decontaminant.
Significance and Impact of the Study
Many bacterial causes of healthcare infection can survive in the inanimate environment for lengthy periods and be transmitted to patients. Furthermore, current methods of environmental decontamination such as detergents, chemical disinfectants or gaseous fumigation are suboptimal for a variety of reasons. We assessed the efficacy of helium and helium–air plasma as a decontaminant and demonstrated a significant reduction in bacterial counts of Staphylococcus aureus on a glass surface. Atomic force microscopy morphologically confirmed the impact on bacterial cells. This approach warrants further study as an alternative to current options for hospital hygiene.
Significance and Impact of the Study: Many bacterial causes of healthcare infection can survive in the inanimate environment for lengthy periods and be transmitted to patients. Furthermore, current methods of environmental decontamination such as detergents, chemical disinfectants or gaseous fumigation are suboptimal for a variety of reasons. We assessed the efficacy of helium and helium–air plasma as a decontaminant and demonstrated a significant reduction in bacterial counts of Staphylococcus aureus on a glass surface. Atomic force microscopy morphologically confirmed the impact on bacterial cells. This approach warrants further study as an alternative to current options for hospital hygiene.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>23607860</pmid><doi>10.1111/lam.12091</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0266-8254 |
| ispartof | Letters in applied microbiology, 2013-08, Vol.57 (2), p.83-90 |
| issn | 0266-8254 1472-765X |
| language | eng |
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| source | Wiley-Blackwell Journals; Oxford University Press Journals |
| subjects | Air Bacterial Load biocides Biological and medical sciences Clostridium difficile Clostridium difficile - drug effects Clostridium difficile - physiology Clostridium difficile - ultrastructure Decontamination - methods Disinfectants - pharmacology disinfection environmental Fundamental and applied biological sciences. Psychology Glass Helium - pharmacology Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - physiology Methicillin-Resistant Staphylococcus aureus - ultrastructure microbial contamination Microbial Viability Microbiology Microscopy, Atomic Force Plasma Gases - pharmacology Spores, Bacterial - drug effects Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Staphylococcus aureus - ultrastructure |
| title | The antimicrobial effects of helium and helium–air plasma on Staphylococcus aureus and Clostridium difficile |
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