Effects of temperature and medium composition on inhibitory activities of gossypol‐related compounds against aflatoxigenic fungi

Aims To investigate the effects of temperature and medium composition on growth/aflatoxin inhibitory activities of terpenoids gossypol, gossypolone and apogossypolone against Aspergillus flavus and A. parasiticus. Methods and Results The compounds were tested at a concentration of 100 μg ml−1 in a C...

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Bibliographic Details
Published in:Journal of applied microbiology 2013-07, Vol.115 (1), p.179-186
Main Authors: Mellon, J.E., Dowd, M.K., Beltz, S.B.
Format: Article
Language:English
Subjects:
aflatoxin biosynthesis inhibition
Aflatoxins - biosynthesis
Agar
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Aspergillus - drug effects
Aspergillus - growth & development
Aspergillus - metabolism
Aspergillus flavus
Aspergillus flavus - drug effects
Aspergillus flavus - growth & development
Aspergillus flavus - metabolism
Aspergillus parasiticus
Biological and medical sciences
Culture Media
Fundamental and applied biological sciences. Psychology
Gossypol - analogs & derivatives
Gossypol - chemistry
Gossypol - pharmacology
Microbiology
natural products
Temperature
terpenoids
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Summary:Aims To investigate the effects of temperature and medium composition on growth/aflatoxin inhibitory activities of terpenoids gossypol, gossypolone and apogossypolone against Aspergillus flavus and A. parasiticus. Methods and Results The compounds were tested at a concentration of 100 μg ml−1 in a Czapek Dox (Czapek) agar medium at 25, 31 and 37°C. Increased incubation temperature marginally increased growth inhibition caused by these compounds, but reduced the aflatoxin inhibition effected by gossypol. Gossypolone and apogossypolone retained good aflatoxin inhibitory activity against A. flavus and A. parasiticus at higher incubation temperatures. However, increased temperature also significantly reduced aflatoxin production in control cultures. The effects of the terpenoids on fungal growth and aflatoxin production against the same fungi were also determined in Czapek, Czapek with a protein/amino acid addendum and yeast extract sucrose (YES) media. Growth of these fungi in the protein‐supplemented Czapek medium or in the YES medium greatly reduced the growth inhibition effects of the terpenoids. Apogossypolone displayed strong anti‐aflatoxigenic activity in the Czapek medium, but this activity was significantly reduced in the protein‐amended Czapek and YES media. Gossypol, which displayed little to no aflatoxin inhibitory activity in the Czapek medium, did yield significant anti‐aflatoxigenic activity in the YES medium. Conclusions Incubation temperature and media composition are important parameters involved in the regulation of aflatoxin production in A. flavus and A. parasiticus. These parameters also affect the potency of growth and aflatoxin inhibitory activities of these gossypol‐related compounds against aflatoxigenic fungi. Significance and Impact of the Study Studies utilizing gossypol‐related compounds as inhibitory agents of biological activities should be interpreted with caution due to compound interaction with multiple components of the test system, especially serum proteins.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12219