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Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines
Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typ...
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| Published in: | Revista brasileira de parasitologia veterinaria 2013-04, Vol.22 (2), p.270-275 |
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| Language: | English |
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| container_end_page | 275 |
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| container_title | Revista brasileira de parasitologia veterinaria |
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| creator | Paulan, Silvana de Cássia Gonzáles, Rutilia Marisela Hernándes Peralta, Laura Adalid Vicentini-Oliveira, Josy Campanhã Biondi, Germano Francisco Conde, Edda Sciuto Parkhouse, Robert Michael Evans Nunes, Cáris Maroni |
| description | Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis. |
| doi_str_mv | 10.1590/s1984-29612013005000026 |
| format | article |
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Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.</description><identifier>EISSN: 1984-2961</identifier><identifier>DOI: 10.1590/s1984-29612013005000026</identifier><identifier>PMID: 23802239</identifier><language>eng</language><publisher>Brazil</publisher><subject>Animals ; Antigens, Helminth - blood ; Cattle - blood ; Cattle Diseases - blood ; Cattle Diseases - diagnosis ; Cysticercosis - blood ; Cysticercosis - diagnosis ; Cysticercosis - veterinary ; Enzyme-Linked Immunosorbent Assay ; Serologic Tests ; Taenia saginata - immunology</subject><ispartof>Revista brasileira de parasitologia veterinaria, 2013-04, Vol.22 (2), p.270-275</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23802239$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paulan, Silvana de Cássia</creatorcontrib><creatorcontrib>Gonzáles, Rutilia Marisela Hernándes</creatorcontrib><creatorcontrib>Peralta, Laura Adalid</creatorcontrib><creatorcontrib>Vicentini-Oliveira, Josy Campanhã</creatorcontrib><creatorcontrib>Biondi, Germano Francisco</creatorcontrib><creatorcontrib>Conde, Edda Sciuto</creatorcontrib><creatorcontrib>Parkhouse, Robert Michael Evans</creatorcontrib><creatorcontrib>Nunes, Cáris Maroni</creatorcontrib><title>Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines</title><title>Revista brasileira de parasitologia veterinaria</title><addtitle>Rev Bras Parasitol Vet</addtitle><description>Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. 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In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.</description><subject>Animals</subject><subject>Antigens, Helminth - blood</subject><subject>Cattle - blood</subject><subject>Cattle Diseases - blood</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cysticercosis - blood</subject><subject>Cysticercosis - diagnosis</subject><subject>Cysticercosis - veterinary</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Serologic Tests</subject><subject>Taenia saginata - immunology</subject><issn>1984-2961</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNo9UM9LwzAYDYK4Of0XNEcv1S9JkzbHMaYOCh7crpavzddRyZrZtML-ewtO3-XxfvAOj7F7AY9CW3iKwuZpIq0REoQC0DBBmgs2_w9m7DrGTwAzJeqKzaTKQUpl5-xjF6kZfUcx8tDwSH3wYd_W6Pm62LwvOcaIJ96Enm-RuhZ5xH3b4YB8CNzRQPXAJzn26P2Jt10zGeR4Fb7bafSGXTboI92eecF2z-vt6jUp3l42q2WRHKUQQyJrmaGWVpsKjEIUlW5yg9qByZSpJQpd6VSTy41LlahTQJtqJalKtSNCtWAPv7vHPnyNFIfy0MaavMeOwhhLoTIJmQWpp-rduTpWB3LlsW8P2J_Kv0_UDz6lYxE</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Paulan, Silvana de Cássia</creator><creator>Gonzáles, Rutilia Marisela Hernándes</creator><creator>Peralta, Laura Adalid</creator><creator>Vicentini-Oliveira, Josy Campanhã</creator><creator>Biondi, Germano Francisco</creator><creator>Conde, Edda Sciuto</creator><creator>Parkhouse, Robert Michael Evans</creator><creator>Nunes, Cáris Maroni</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20130401</creationdate><title>Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines</title><author>Paulan, Silvana de Cássia ; Gonzáles, Rutilia Marisela Hernándes ; Peralta, Laura Adalid ; Vicentini-Oliveira, Josy Campanhã ; Biondi, Germano Francisco ; Conde, Edda Sciuto ; Parkhouse, Robert Michael Evans ; Nunes, Cáris Maroni</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-2c27a52956b063aa1b5f86a5d06736c2a15b545ed86d431c40a94532eb45deea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antigens, Helminth - blood</topic><topic>Cattle - blood</topic><topic>Cattle Diseases - blood</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cysticercosis - blood</topic><topic>Cysticercosis - diagnosis</topic><topic>Cysticercosis - veterinary</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Serologic Tests</topic><topic>Taenia saginata - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paulan, Silvana de Cássia</creatorcontrib><creatorcontrib>Gonzáles, Rutilia Marisela Hernándes</creatorcontrib><creatorcontrib>Peralta, Laura Adalid</creatorcontrib><creatorcontrib>Vicentini-Oliveira, Josy Campanhã</creatorcontrib><creatorcontrib>Biondi, Germano Francisco</creatorcontrib><creatorcontrib>Conde, Edda Sciuto</creatorcontrib><creatorcontrib>Parkhouse, Robert Michael Evans</creatorcontrib><creatorcontrib>Nunes, Cáris Maroni</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revista brasileira de parasitologia veterinaria</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paulan, Silvana de Cássia</au><au>Gonzáles, Rutilia Marisela Hernándes</au><au>Peralta, Laura Adalid</au><au>Vicentini-Oliveira, Josy Campanhã</au><au>Biondi, Germano Francisco</au><au>Conde, Edda Sciuto</au><au>Parkhouse, Robert Michael Evans</au><au>Nunes, Cáris Maroni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines</atitle><jtitle>Revista brasileira de parasitologia veterinaria</jtitle><addtitle>Rev Bras Parasitol Vet</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>22</volume><issue>2</issue><spage>270</spage><epage>275</epage><pages>270-275</pages><eissn>1984-2961</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.</abstract><cop>Brazil</cop><pmid>23802239</pmid><doi>10.1590/s1984-29612013005000026</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 1984-2961 |
| ispartof | Revista brasileira de parasitologia veterinaria, 2013-04, Vol.22 (2), p.270-275 |
| issn | 1984-2961 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1372079025 |
| source | SciELO Brazil |
| subjects | Animals Antigens, Helminth - blood Cattle - blood Cattle Diseases - blood Cattle Diseases - diagnosis Cysticercosis - blood Cysticercosis - diagnosis Cysticercosis - veterinary Enzyme-Linked Immunosorbent Assay Serologic Tests Taenia saginata - immunology |
| title | Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines |
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