Long-term effects of selected xenobiotica on freshwater green algae: development of a flow-through test system

A flow-through system was developed to evaluate the effect of volatile and unstable substances on freshwater green algae using Chlamydomonas reinhardi as test organism. EC10 was measured over 4-10 d. Algae were constrained to the lower portion of the incubation vessel by a 1.2 um cellulose acetate m...

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Published in:The Science of the total environment 1993-01, Vol.Supplement, p.735-740
Main Authors: Schafer, H, Wenzel, A, Fritsche, U, Roderer, G, Traunspurger, W
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Wenzel, A
Fritsche, U
Roderer, G
Traunspurger, W
description A flow-through system was developed to evaluate the effect of volatile and unstable substances on freshwater green algae using Chlamydomonas reinhardi as test organism. EC10 was measured over 4-10 d. Algae were constrained to the lower portion of the incubation vessel by a 1.2 um cellulose acetate membrane. Hollow fibre membranes removed gases and introduced carbon dioxide. Flow rate was 2 cell volumes per d; the pH was kept at 6-7 and cell density 50,000 per ml. EC50 values for the tested substances rose in the order atrazine, copper, cadmium, pentachlorophenol, 3,4-dichloroaniline, lindane, methyl parathion and 1,2-dichloropropane; the range for 10 d EC50 was 10.2-50,000 ug per litre. Sensitivity was greater in the flow-through system than in static tests. Test substance concentrations were consistent over the period of the test.
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title Long-term effects of selected xenobiotica on freshwater green algae: development of a flow-through test system
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