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RNA-Seq: revelation of the messengers
Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and c...
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| Published in: | Trends in plant science 2013-04, Vol.18 (4), p.175-179 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c493t-1986e15e3556e83d6ddc5d35fa87e038898132f1474a5d768b05c6f704604beb3 |
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| cites | cdi_FETCH-LOGICAL-c493t-1986e15e3556e83d6ddc5d35fa87e038898132f1474a5d768b05c6f704604beb3 |
| container_end_page | 179 |
| container_issue | 4 |
| container_start_page | 175 |
| container_title | Trends in plant science |
| container_volume | 18 |
| creator | Van Verk, Marcel C Hickman, Richard Pieterse, Corné M.J Van Wees, Saskia C.M |
| description | Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and considerations that are critical for RNA-Seq data analysis and provide a generic tutorial with example data that outlines the whole pipeline from next-generation sequencing output to quantification of differential gene expression. |
| doi_str_mv | 10.1016/j.tplants.2013.02.001 |
| format | article |
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| ispartof | Trends in plant science, 2013-04, Vol.18 (4), p.175-179 |
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| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Base Sequence bioinformatics biologists Computational Biology DNA, Complementary - genetics Gene Expression Profiling - methods gene expression regulation Gene Library High-Throughput Nucleotide Sequencing - methods microarray technology RNA, Messenger - genetics Sequence Analysis, RNA - methods Transcriptome |
| title | RNA-Seq: revelation of the messengers |
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