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Fingerprint-Imprinted Polymer: Rational Selection of Peptide Epitope Templates for the Determination of Proteins by Molecularly Imprinted Polymers
The pool of peptides composing a protein allows for its distinctive identification in a process named fingerprint (FP) analysis. Here, the FP concept is used to develop a method for the rational preparation of molecularly imprinted polymers (MIPs) for protein recognition. The fingerprint imprinting...
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Published in: | Analytical chemistry (Washington) 2012-05, Vol.84 (9), p.4036-4041 |
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description | The pool of peptides composing a protein allows for its distinctive identification in a process named fingerprint (FP) analysis. Here, the FP concept is used to develop a method for the rational preparation of molecularly imprinted polymers (MIPs) for protein recognition. The fingerprint imprinting (FIP) is based on the following: (1) the in silico cleavage of the protein sequence of interest with specific agents; (2) the screening of all the peptide sequences generated against the UniProtKB database in order to allow for the rational selection of distinctive and unique peptides (named as epitopes) of the target protein; (3) the selected epitopes are synthesized and used as templates for the molecular imprinting process. To prove the principle, NT-proBNP, a marker of the risk of cardiovascular events, was chosen as an example. The in silico analysis of the NT-proBNP sequence allowed us to individuate the peptide candidates, which were next used as templates for the preparation of NT-pro-BNP-specific FIPs and tested for their ability to bind the NT-proBNP peptides in complex samples. Results indicated an imprinting factor, IF, of ∼10, a binding capacity of 0.5–2 mg/g, and the ability to rebind 40% of the template in a complex sample, composed of the whole digests of NT-proBNP. |
doi_str_mv | 10.1021/ac203422r |
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Here, the FP concept is used to develop a method for the rational preparation of molecularly imprinted polymers (MIPs) for protein recognition. The fingerprint imprinting (FIP) is based on the following: (1) the in silico cleavage of the protein sequence of interest with specific agents; (2) the screening of all the peptide sequences generated against the UniProtKB database in order to allow for the rational selection of distinctive and unique peptides (named as epitopes) of the target protein; (3) the selected epitopes are synthesized and used as templates for the molecular imprinting process. To prove the principle, NT-proBNP, a marker of the risk of cardiovascular events, was chosen as an example. The in silico analysis of the NT-proBNP sequence allowed us to individuate the peptide candidates, which were next used as templates for the preparation of NT-pro-BNP-specific FIPs and tested for their ability to bind the NT-proBNP peptides in complex samples. 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Results indicated an imprinting factor, IF, of ∼10, a binding capacity of 0.5–2 mg/g, and the ability to rebind 40% of the template in a complex sample, composed of the whole digests of NT-proBNP.</description><subject>Amino Acid Sequence</subject><subject>Analytical chemistry</subject><subject>Cardiovascular Diseases - diagnosis</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Molecular Imprinting - methods</subject><subject>Molecules</subject><subject>Natriuretic Peptide, Brain - blood</subject><subject>Natriuretic Peptide, Brain - isolation & purification</subject><subject>Natriuretic Peptide, Brain - metabolism</subject><subject>Peptide Fragments - blood</subject><subject>Peptide Fragments - isolation & purification</subject><subject>Peptide Fragments - metabolism</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Peptides - metabolism</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Polymers - metabolism</subject><subject>Protein Binding</subject><subject>Proteins</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpl0c1u1DAQAGALgeiycOAFkCWEVA6BsR07CTfUH6hURAV7j7zOGFw5cbCdw74GT4zbLltULh4fvpmxZwh5yeAdA87ea8NB1JzHR2TFJIdKtS1_TFYAICreAByRZyldAzAGTD0lR5zXistarcjvczf9wDhHN-XqYryNONCr4Hcjxg_0m84uTNrT7-jR3NxpsPQK5-wGpGezy2FGusFx9jpjojZEmn8iPcWMcXSTPqTEkNFNiW539EsotRavo9_R_3qm5-SJ1T7hi31ck8352ebkc3X59dPFycfLSotG5MoOndwKMdi6rWEABVw2rJOdFGg0yNbYpgaUdTd0VtgttMi7TksYBNclS6zJ8V3ZOYZfC6bcjy4Z9F5PGJbUl1GBEkqVY01eP6DXYYllKreK1YVJKOrtnTIxpBTR9uVno467gvqbPfWHPRX7al9x2Y44HOTfxRTwZg90MtrbqCfj0r2TrSqLbe6dNunfVz1s-Ae8r6el</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Bossi, Alessandra M</creator><creator>Sharma, Piyush S</creator><creator>Montana, Luca</creator><creator>Zoccatelli, Gianni</creator><creator>Laub, Orgad</creator><creator>Levi, Raphael</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Fingerprint-Imprinted Polymer: Rational Selection of Peptide Epitope Templates for the Determination of Proteins by Molecularly Imprinted Polymers</title><author>Bossi, Alessandra M ; Sharma, Piyush S ; Montana, Luca ; Zoccatelli, Gianni ; Laub, Orgad ; Levi, Raphael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a373t-fd95b33df4840d060257195953eca058cf740e549d9f3fb08e299a50d32a3df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical chemistry</topic><topic>Cardiovascular Diseases - diagnosis</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Molecular Imprinting - methods</topic><topic>Molecules</topic><topic>Natriuretic Peptide, Brain - blood</topic><topic>Natriuretic Peptide, Brain - isolation & purification</topic><topic>Natriuretic Peptide, Brain - metabolism</topic><topic>Peptide Fragments - blood</topic><topic>Peptide Fragments - isolation & purification</topic><topic>Peptide Fragments - metabolism</topic><topic>Peptides</topic><topic>Peptides - chemistry</topic><topic>Peptides - metabolism</topic><topic>Polymers</topic><topic>Polymers - chemistry</topic><topic>Polymers - metabolism</topic><topic>Protein Binding</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bossi, Alessandra M</creatorcontrib><creatorcontrib>Sharma, Piyush S</creatorcontrib><creatorcontrib>Montana, Luca</creatorcontrib><creatorcontrib>Zoccatelli, Gianni</creatorcontrib><creatorcontrib>Laub, Orgad</creatorcontrib><creatorcontrib>Levi, Raphael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bossi, Alessandra M</au><au>Sharma, Piyush S</au><au>Montana, Luca</au><au>Zoccatelli, Gianni</au><au>Laub, Orgad</au><au>Levi, Raphael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fingerprint-Imprinted Polymer: Rational Selection of Peptide Epitope Templates for the Determination of Proteins by Molecularly Imprinted Polymers</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>84</volume><issue>9</issue><spage>4036</spage><epage>4041</epage><pages>4036-4041</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The pool of peptides composing a protein allows for its distinctive identification in a process named fingerprint (FP) analysis. Here, the FP concept is used to develop a method for the rational preparation of molecularly imprinted polymers (MIPs) for protein recognition. The fingerprint imprinting (FIP) is based on the following: (1) the in silico cleavage of the protein sequence of interest with specific agents; (2) the screening of all the peptide sequences generated against the UniProtKB database in order to allow for the rational selection of distinctive and unique peptides (named as epitopes) of the target protein; (3) the selected epitopes are synthesized and used as templates for the molecular imprinting process. To prove the principle, NT-proBNP, a marker of the risk of cardiovascular events, was chosen as an example. The in silico analysis of the NT-proBNP sequence allowed us to individuate the peptide candidates, which were next used as templates for the preparation of NT-pro-BNP-specific FIPs and tested for their ability to bind the NT-proBNP peptides in complex samples. Results indicated an imprinting factor, IF, of ∼10, a binding capacity of 0.5–2 mg/g, and the ability to rebind 40% of the template in a complex sample, composed of the whole digests of NT-proBNP.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22462546</pmid><doi>10.1021/ac203422r</doi><tpages>6</tpages></addata></record> |
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subjects | Amino Acid Sequence Analytical chemistry Cardiovascular Diseases - diagnosis Chemistry Exact sciences and technology Humans Molecular Imprinting - methods Molecules Natriuretic Peptide, Brain - blood Natriuretic Peptide, Brain - isolation & purification Natriuretic Peptide, Brain - metabolism Peptide Fragments - blood Peptide Fragments - isolation & purification Peptide Fragments - metabolism Peptides Peptides - chemistry Peptides - metabolism Polymers Polymers - chemistry Polymers - metabolism Protein Binding Proteins |
title | Fingerprint-Imprinted Polymer: Rational Selection of Peptide Epitope Templates for the Determination of Proteins by Molecularly Imprinted Polymers |
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