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Histerocystoplasty: A Novel Surgical Procedure in the Rat
Background Enterocystoplasties are associated to complications. To avoid them, different types of tissue templates have been used to augment the bladder and induce native bladder regeneration. Materials and Methods A novel surgical technique for bladder reconstruction using autologous uterine tissue...
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Published in: | The Journal of surgical research 2012-06, Vol.175 (1), p.157-162 |
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creator | Dapena, Lidia, D.V.M., Ph.D Dapena, Isabel, M.D Regadera, Javier, M.D., Ph.D Gaspar, María Jesús, Pharm, Ph.D González-Peramato, Pilar, M.D., Ph.D |
description | Background Enterocystoplasties are associated to complications. To avoid them, different types of tissue templates have been used to augment the bladder and induce native bladder regeneration. Materials and Methods A novel surgical technique for bladder reconstruction using autologous uterine tissue was evaluated in a rat model. Forty-two female Wistar rats were randomly allocated into three groups: sham-operation hysterocystorrhaphy ( n = 12), hysterocystoplasty ( n = 18), and control ( n = 12). Two weeks after surgery, ultrasound examination of the bladder was performed. At 2, 4, or 6 mo after surgery, the rats were anesthetized and blood and urine samples were taken. They were then euthanized and post-mortem and histologic examination were performed. Ultrasound examination, analytical parameters and weight control, as well as gross and histologic examination were performed in all the operated animals. The statistical analysis was performed using Kruskal-Wallis and the extension of Fisher’s exact tests. Significance was set at 5% ( P < 0.05). Results Serum chemistry, blood count and peripheral blood smears, electrolytes, and urinary parameters were all within the normal range for the rat. Histologic sections of the surgically augmented zone between the bladder and uterine horn demonstrated urothelial epithelization, providing adequate coverage of the transition area in 72.22% of the rats that underwent hysterocystoplasty. Conclusions The hysterocystoplasty was technically viable in all the cases and proved to be an easy and safe surgical model for bladder reconstruction. All animals were healthy after surgery and all systemic parameters analyzed were within normal physiologic range for the rat. |
doi_str_mv | 10.1016/j.jss.2011.03.002 |
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To avoid them, different types of tissue templates have been used to augment the bladder and induce native bladder regeneration. Materials and Methods A novel surgical technique for bladder reconstruction using autologous uterine tissue was evaluated in a rat model. Forty-two female Wistar rats were randomly allocated into three groups: sham-operation hysterocystorrhaphy ( n = 12), hysterocystoplasty ( n = 18), and control ( n = 12). Two weeks after surgery, ultrasound examination of the bladder was performed. At 2, 4, or 6 mo after surgery, the rats were anesthetized and blood and urine samples were taken. They were then euthanized and post-mortem and histologic examination were performed. Ultrasound examination, analytical parameters and weight control, as well as gross and histologic examination were performed in all the operated animals. The statistical analysis was performed using Kruskal-Wallis and the extension of Fisher’s exact tests. Significance was set at 5% ( P < 0.05). Results Serum chemistry, blood count and peripheral blood smears, electrolytes, and urinary parameters were all within the normal range for the rat. Histologic sections of the surgically augmented zone between the bladder and uterine horn demonstrated urothelial epithelization, providing adequate coverage of the transition area in 72.22% of the rats that underwent hysterocystoplasty. Conclusions The hysterocystoplasty was technically viable in all the cases and proved to be an easy and safe surgical model for bladder reconstruction. All animals were healthy after surgery and all systemic parameters analyzed were within normal physiologic range for the rat.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2011.03.002</identifier><identifier>PMID: 21529834</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; augmentation ; bladder ; Feasibility Studies ; Female ; hysterocystoplasty ; Muscle, Smooth - transplantation ; Rats ; Rats, Wistar ; Reconstructive Surgical Procedures ; Surgery ; surgical model ; Urinary Bladder - surgery ; Urologic Surgical Procedures - methods ; uterus ; Uterus - transplantation</subject><ispartof>The Journal of surgical research, 2012-06, Vol.175 (1), p.157-162</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-17666e205e0477071f2b1ff376f15e92a35271dfc1596092f2c1530b4450e11c3</citedby><cites>FETCH-LOGICAL-c408t-17666e205e0477071f2b1ff376f15e92a35271dfc1596092f2c1530b4450e11c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21529834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dapena, Lidia, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Dapena, Isabel, M.D</creatorcontrib><creatorcontrib>Regadera, Javier, M.D., Ph.D</creatorcontrib><creatorcontrib>Gaspar, María Jesús, Pharm, Ph.D</creatorcontrib><creatorcontrib>González-Peramato, Pilar, M.D., Ph.D</creatorcontrib><title>Histerocystoplasty: A Novel Surgical Procedure in the Rat</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Background Enterocystoplasties are associated to complications. To avoid them, different types of tissue templates have been used to augment the bladder and induce native bladder regeneration. Materials and Methods A novel surgical technique for bladder reconstruction using autologous uterine tissue was evaluated in a rat model. Forty-two female Wistar rats were randomly allocated into three groups: sham-operation hysterocystorrhaphy ( n = 12), hysterocystoplasty ( n = 18), and control ( n = 12). Two weeks after surgery, ultrasound examination of the bladder was performed. At 2, 4, or 6 mo after surgery, the rats were anesthetized and blood and urine samples were taken. They were then euthanized and post-mortem and histologic examination were performed. Ultrasound examination, analytical parameters and weight control, as well as gross and histologic examination were performed in all the operated animals. The statistical analysis was performed using Kruskal-Wallis and the extension of Fisher’s exact tests. Significance was set at 5% ( P < 0.05). Results Serum chemistry, blood count and peripheral blood smears, electrolytes, and urinary parameters were all within the normal range for the rat. Histologic sections of the surgically augmented zone between the bladder and uterine horn demonstrated urothelial epithelization, providing adequate coverage of the transition area in 72.22% of the rats that underwent hysterocystoplasty. Conclusions The hysterocystoplasty was technically viable in all the cases and proved to be an easy and safe surgical model for bladder reconstruction. All animals were healthy after surgery and all systemic parameters analyzed were within normal physiologic range for the rat.</description><subject>Animals</subject><subject>augmentation</subject><subject>bladder</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>hysterocystoplasty</subject><subject>Muscle, Smooth - transplantation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reconstructive Surgical Procedures</subject><subject>Surgery</subject><subject>surgical model</subject><subject>Urinary Bladder - surgery</subject><subject>Urologic Surgical Procedures - methods</subject><subject>uterus</subject><subject>Uterus - transplantation</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kU1P3DAQhq2qVVmW_oBeUI69JJ3xR7ymUiWEoCAhqLr0bGW9k-KQTRY7Qdp_j6MFDhx68oee95XmGca-IhQIWH5viibGggNiAaIA4B_YDMGofFFq8ZHN0g_P5QLkATuMsYH0Nlp8ZgccFTcLIWfMXPo4UOjdLg79tq3isDvJTrOb_onabDmGf95VbfY7AbQeA2W-y4Z7yv5UwxH7VFdtpC8v55z9vTi_O7vMr29_XZ2dXudOwmLIUZdlSRwUgdQaNNZ8hXUtdFmjIsMrobjGde1QmRIMr3m6CVhJqYAQnZizb_vebegfR4qD3fjoqG2rjvox2mQCpFFS6YTiHnWhjzFQbbfBb6qwS9DElbaxyZidjFkQNvlImeOX-nG1ofVb4lVRAn7sAUpDPnkKNjpPXfLhA7nBrnv_3_qf79Ku9d0k9YF2FJt-DF2yZ9FGbsEup5VNG0MEQKNRPANWDo3O</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Dapena, Lidia, D.V.M., Ph.D</creator><creator>Dapena, Isabel, M.D</creator><creator>Regadera, Javier, M.D., Ph.D</creator><creator>Gaspar, María Jesús, Pharm, Ph.D</creator><creator>González-Peramato, Pilar, M.D., Ph.D</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120601</creationdate><title>Histerocystoplasty: A Novel Surgical Procedure in the Rat</title><author>Dapena, Lidia, D.V.M., Ph.D ; Dapena, Isabel, M.D ; Regadera, Javier, M.D., Ph.D ; Gaspar, María Jesús, Pharm, Ph.D ; González-Peramato, Pilar, M.D., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-17666e205e0477071f2b1ff376f15e92a35271dfc1596092f2c1530b4450e11c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>augmentation</topic><topic>bladder</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>hysterocystoplasty</topic><topic>Muscle, Smooth - transplantation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reconstructive Surgical Procedures</topic><topic>Surgery</topic><topic>surgical model</topic><topic>Urinary Bladder - surgery</topic><topic>Urologic Surgical Procedures - methods</topic><topic>uterus</topic><topic>Uterus - transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dapena, Lidia, D.V.M., Ph.D</creatorcontrib><creatorcontrib>Dapena, Isabel, M.D</creatorcontrib><creatorcontrib>Regadera, Javier, M.D., Ph.D</creatorcontrib><creatorcontrib>Gaspar, María Jesús, Pharm, Ph.D</creatorcontrib><creatorcontrib>González-Peramato, Pilar, M.D., Ph.D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dapena, Lidia, D.V.M., Ph.D</au><au>Dapena, Isabel, M.D</au><au>Regadera, Javier, M.D., Ph.D</au><au>Gaspar, María Jesús, Pharm, Ph.D</au><au>González-Peramato, Pilar, M.D., Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histerocystoplasty: A Novel Surgical Procedure in the Rat</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>175</volume><issue>1</issue><spage>157</spage><epage>162</epage><pages>157-162</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Background Enterocystoplasties are associated to complications. To avoid them, different types of tissue templates have been used to augment the bladder and induce native bladder regeneration. Materials and Methods A novel surgical technique for bladder reconstruction using autologous uterine tissue was evaluated in a rat model. Forty-two female Wistar rats were randomly allocated into three groups: sham-operation hysterocystorrhaphy ( n = 12), hysterocystoplasty ( n = 18), and control ( n = 12). Two weeks after surgery, ultrasound examination of the bladder was performed. At 2, 4, or 6 mo after surgery, the rats were anesthetized and blood and urine samples were taken. They were then euthanized and post-mortem and histologic examination were performed. Ultrasound examination, analytical parameters and weight control, as well as gross and histologic examination were performed in all the operated animals. The statistical analysis was performed using Kruskal-Wallis and the extension of Fisher’s exact tests. Significance was set at 5% ( P < 0.05). Results Serum chemistry, blood count and peripheral blood smears, electrolytes, and urinary parameters were all within the normal range for the rat. Histologic sections of the surgically augmented zone between the bladder and uterine horn demonstrated urothelial epithelization, providing adequate coverage of the transition area in 72.22% of the rats that underwent hysterocystoplasty. Conclusions The hysterocystoplasty was technically viable in all the cases and proved to be an easy and safe surgical model for bladder reconstruction. All animals were healthy after surgery and all systemic parameters analyzed were within normal physiologic range for the rat.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21529834</pmid><doi>10.1016/j.jss.2011.03.002</doi><tpages>6</tpages></addata></record> |
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subjects | Animals augmentation bladder Feasibility Studies Female hysterocystoplasty Muscle, Smooth - transplantation Rats Rats, Wistar Reconstructive Surgical Procedures Surgery surgical model Urinary Bladder - surgery Urologic Surgical Procedures - methods uterus Uterus - transplantation |
title | Histerocystoplasty: A Novel Surgical Procedure in the Rat |
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