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Investigation of genes and miRNAs associated with scarless healing in orbital adipose‐derived and limbal‐derived mesenchymal stem cells treated with vitamin C
Aims/Purpose: In this study we aimed to investigate miRNAs associated with scarless wound healing in limbal‐derived stomal‐mesenchymal stem cells (hLMSC) and orbital adipose tissue‐derived stem cells (hOA‐MSC) treated individually with vitamin C and exosomes obtained after vitamin C treatment. Metho...
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| Published in: | Acta ophthalmologica (Oxford, England) England), 2024-01, Vol.102 (S279), p.n/a |
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| container_title | Acta ophthalmologica (Oxford, England) |
| container_volume | 102 |
| creator | Atalay, Eray Eyubova, Sevinj Soykan, Merve Nur Tasa, Burcugül Altuğ Ozalp, Onur Sarıboyacı, Ayla Eker |
| description | Aims/Purpose: In this study we aimed to investigate miRNAs associated with scarless wound healing in limbal‐derived stomal‐mesenchymal stem cells (hLMSC) and orbital adipose tissue‐derived stem cells (hOA‐MSC) treated individually with vitamin C and exosomes obtained after vitamin C treatment.
Methods: Isolation and characterization of hLMSCs and hOA‐MSCs were performed. MTT assay was employed to determine the ideal non‐cytotoxic and proliferative concentration of vitamin C (vit C). The selected concentration was applied to the cell culture medium of hLMSCs and hOA‐MSCs (vit C‐treated) followed by procurement of secreted exosomes (vit C‐exo) and their characterization. These exosomes were then used to enrich cell culture media of naïve hLMSCs and hOA‐MSCs. miR29, miR107, miR155, and miR543 miRNAs and Transforming Growth Factor Beta 1 (TGFβ1), TGFβ3 were analysed by Real‐Time PCR in both vit C treated and vit C‐exo‐treated cells.
Results: Vit C‐exo and vit C‐treated hLMSCs demonstrated slight reductions in TGFβ1 and TGFβ3 expressions compared to controls. In contrast, a modest increase in TGFβ1 and TGFβ3 levels was noted in hOA‐MSCs after vit‐C and vit C‐exo treatment. After vit C‐exo application, expression of miR155 and miR107 increased in hLMSCs and decreased in hOA‐MSCs. An opposite trend was observed with miR543 expression with an increase in hOA‐MSCs and a decrease in hLMSCs. miR29 associated with fibrosis was dramatically increased in hOA‐MSCs, but not in hLMSCs.
Conclusions: In conclusion, we show that miRNA and RNA contents of treated mesenchymal stem cells may vary depending on the origin of tissue. These results suggest that vitamin C could promote a relatively more pronounced anti‐fibrotic character in hOA‐MSCs.
The authors acknowledge the support from Eskisehir Osmangazi University, Scientific Research Projects (ESOGU‐BAP Grant ID: TOA‐2022‐2458). |
| doi_str_mv | 10.1111/aos.16067 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_3089657646</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3089657646</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1377-8f279edc7bd2b1b6ee9ba4f9a81634541cd177e56187a7a4fe15a936751b3acf3</originalsourceid><addsrcrecordid>eNp1kU1OwzAQhSMEEqWw4AaWWLFoGzexnSyrip9KFZX4kdhFjjNpXTlx8aStuuMInIGjcRJcgoAN3ng087030rwgOKdhn_o3kBb7lIdcHAQdKhjrRYInhz81ez4OThCXYcgp53EneJ_UG8BGz2WjbU1sSeZQAxJZF6TS93cjXyJapWUDBdnqZkFQSWcAkSxAGl3PifY6l-tGGiILvbIIH69vBTi98ZK9kdFVLs2fZgUItVrsKi_BBiqiwBgkjYPfNRtvWHnr8WlwVEqDcPb9d4On66vH8W1vOruZjEfTnqKREL2kHIoUCiXyYpjTnAOkuYzLVCaURzGLqSqoEMA4TYQUfgKUyTTigtE8kqqMusFF67ty9mXtj5It7drVfmUWhUnKmeAx99RlSylnER2U2crpSrpdRsNsH0HmI8i-IvDsoGW32sDufzAbzR5axSfwP43j</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3089657646</pqid></control><display><type>article</type><title>Investigation of genes and miRNAs associated with scarless healing in orbital adipose‐derived and limbal‐derived mesenchymal stem cells treated with vitamin C</title><source>Wiley</source><creator>Atalay, Eray ; Eyubova, Sevinj ; Soykan, Merve Nur ; Tasa, Burcugül Altuğ ; Ozalp, Onur ; Sarıboyacı, Ayla Eker</creator><creatorcontrib>Atalay, Eray ; Eyubova, Sevinj ; Soykan, Merve Nur ; Tasa, Burcugül Altuğ ; Ozalp, Onur ; Sarıboyacı, Ayla Eker</creatorcontrib><description>Aims/Purpose: In this study we aimed to investigate miRNAs associated with scarless wound healing in limbal‐derived stomal‐mesenchymal stem cells (hLMSC) and orbital adipose tissue‐derived stem cells (hOA‐MSC) treated individually with vitamin C and exosomes obtained after vitamin C treatment.
Methods: Isolation and characterization of hLMSCs and hOA‐MSCs were performed. MTT assay was employed to determine the ideal non‐cytotoxic and proliferative concentration of vitamin C (vit C). The selected concentration was applied to the cell culture medium of hLMSCs and hOA‐MSCs (vit C‐treated) followed by procurement of secreted exosomes (vit C‐exo) and their characterization. These exosomes were then used to enrich cell culture media of naïve hLMSCs and hOA‐MSCs. miR29, miR107, miR155, and miR543 miRNAs and Transforming Growth Factor Beta 1 (TGFβ1), TGFβ3 were analysed by Real‐Time PCR in both vit C treated and vit C‐exo‐treated cells.
Results: Vit C‐exo and vit C‐treated hLMSCs demonstrated slight reductions in TGFβ1 and TGFβ3 expressions compared to controls. In contrast, a modest increase in TGFβ1 and TGFβ3 levels was noted in hOA‐MSCs after vit‐C and vit C‐exo treatment. After vit C‐exo application, expression of miR155 and miR107 increased in hLMSCs and decreased in hOA‐MSCs. An opposite trend was observed with miR543 expression with an increase in hOA‐MSCs and a decrease in hLMSCs. miR29 associated with fibrosis was dramatically increased in hOA‐MSCs, but not in hLMSCs.
Conclusions: In conclusion, we show that miRNA and RNA contents of treated mesenchymal stem cells may vary depending on the origin of tissue. These results suggest that vitamin C could promote a relatively more pronounced anti‐fibrotic character in hOA‐MSCs.
The authors acknowledge the support from Eskisehir Osmangazi University, Scientific Research Projects (ESOGU‐BAP Grant ID: TOA‐2022‐2458).</description><identifier>ISSN: 1755-375X</identifier><identifier>EISSN: 1755-3768</identifier><identifier>DOI: 10.1111/aos.16067</identifier><language>eng</language><publisher>Malden: Wiley Subscription Services, Inc</publisher><subject>Adipose tissue ; Ascorbic acid ; Cell culture ; Culture media ; Cytotoxicity ; Exosomes ; Fibrosis ; Mesenchymal stem cells ; MicroRNAs ; miRNA ; Stem cells ; Transforming growth factor-b1 ; Vitamin C ; Wound healing</subject><ispartof>Acta ophthalmologica (Oxford, England), 2024-01, Vol.102 (S279), p.n/a</ispartof><rights>2024 The Authors Acta Ophthalmologica © 2024 Acta Ophthalmologica Scandinavica Foundation</rights><rights>Copyright © 2024 Acta Ophthalmologica Scandinavica Foundation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faos.16067$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,51032</link.rule.ids></links><search><creatorcontrib>Atalay, Eray</creatorcontrib><creatorcontrib>Eyubova, Sevinj</creatorcontrib><creatorcontrib>Soykan, Merve Nur</creatorcontrib><creatorcontrib>Tasa, Burcugül Altuğ</creatorcontrib><creatorcontrib>Ozalp, Onur</creatorcontrib><creatorcontrib>Sarıboyacı, Ayla Eker</creatorcontrib><title>Investigation of genes and miRNAs associated with scarless healing in orbital adipose‐derived and limbal‐derived mesenchymal stem cells treated with vitamin C</title><title>Acta ophthalmologica (Oxford, England)</title><description>Aims/Purpose: In this study we aimed to investigate miRNAs associated with scarless wound healing in limbal‐derived stomal‐mesenchymal stem cells (hLMSC) and orbital adipose tissue‐derived stem cells (hOA‐MSC) treated individually with vitamin C and exosomes obtained after vitamin C treatment.
Methods: Isolation and characterization of hLMSCs and hOA‐MSCs were performed. MTT assay was employed to determine the ideal non‐cytotoxic and proliferative concentration of vitamin C (vit C). The selected concentration was applied to the cell culture medium of hLMSCs and hOA‐MSCs (vit C‐treated) followed by procurement of secreted exosomes (vit C‐exo) and their characterization. These exosomes were then used to enrich cell culture media of naïve hLMSCs and hOA‐MSCs. miR29, miR107, miR155, and miR543 miRNAs and Transforming Growth Factor Beta 1 (TGFβ1), TGFβ3 were analysed by Real‐Time PCR in both vit C treated and vit C‐exo‐treated cells.
Results: Vit C‐exo and vit C‐treated hLMSCs demonstrated slight reductions in TGFβ1 and TGFβ3 expressions compared to controls. In contrast, a modest increase in TGFβ1 and TGFβ3 levels was noted in hOA‐MSCs after vit‐C and vit C‐exo treatment. After vit C‐exo application, expression of miR155 and miR107 increased in hLMSCs and decreased in hOA‐MSCs. An opposite trend was observed with miR543 expression with an increase in hOA‐MSCs and a decrease in hLMSCs. miR29 associated with fibrosis was dramatically increased in hOA‐MSCs, but not in hLMSCs.
Conclusions: In conclusion, we show that miRNA and RNA contents of treated mesenchymal stem cells may vary depending on the origin of tissue. These results suggest that vitamin C could promote a relatively more pronounced anti‐fibrotic character in hOA‐MSCs.
The authors acknowledge the support from Eskisehir Osmangazi University, Scientific Research Projects (ESOGU‐BAP Grant ID: TOA‐2022‐2458).</description><subject>Adipose tissue</subject><subject>Ascorbic acid</subject><subject>Cell culture</subject><subject>Culture media</subject><subject>Cytotoxicity</subject><subject>Exosomes</subject><subject>Fibrosis</subject><subject>Mesenchymal stem cells</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Stem cells</subject><subject>Transforming growth factor-b1</subject><subject>Vitamin C</subject><subject>Wound healing</subject><issn>1755-375X</issn><issn>1755-3768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kU1OwzAQhSMEEqWw4AaWWLFoGzexnSyrip9KFZX4kdhFjjNpXTlx8aStuuMInIGjcRJcgoAN3ng087030rwgOKdhn_o3kBb7lIdcHAQdKhjrRYInhz81ez4OThCXYcgp53EneJ_UG8BGz2WjbU1sSeZQAxJZF6TS93cjXyJapWUDBdnqZkFQSWcAkSxAGl3PifY6l-tGGiILvbIIH69vBTi98ZK9kdFVLs2fZgUItVrsKi_BBiqiwBgkjYPfNRtvWHnr8WlwVEqDcPb9d4On66vH8W1vOruZjEfTnqKREL2kHIoUCiXyYpjTnAOkuYzLVCaURzGLqSqoEMA4TYQUfgKUyTTigtE8kqqMusFF67ty9mXtj5It7drVfmUWhUnKmeAx99RlSylnER2U2crpSrpdRsNsH0HmI8i-IvDsoGW32sDufzAbzR5axSfwP43j</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Atalay, Eray</creator><creator>Eyubova, Sevinj</creator><creator>Soykan, Merve Nur</creator><creator>Tasa, Burcugül Altuğ</creator><creator>Ozalp, Onur</creator><creator>Sarıboyacı, Ayla Eker</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>202401</creationdate><title>Investigation of genes and miRNAs associated with scarless healing in orbital adipose‐derived and limbal‐derived mesenchymal stem cells treated with vitamin C</title><author>Atalay, Eray ; Eyubova, Sevinj ; Soykan, Merve Nur ; Tasa, Burcugül Altuğ ; Ozalp, Onur ; Sarıboyacı, Ayla Eker</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1377-8f279edc7bd2b1b6ee9ba4f9a81634541cd177e56187a7a4fe15a936751b3acf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adipose tissue</topic><topic>Ascorbic acid</topic><topic>Cell culture</topic><topic>Culture media</topic><topic>Cytotoxicity</topic><topic>Exosomes</topic><topic>Fibrosis</topic><topic>Mesenchymal stem cells</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Stem cells</topic><topic>Transforming growth factor-b1</topic><topic>Vitamin C</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atalay, Eray</creatorcontrib><creatorcontrib>Eyubova, Sevinj</creatorcontrib><creatorcontrib>Soykan, Merve Nur</creatorcontrib><creatorcontrib>Tasa, Burcugül Altuğ</creatorcontrib><creatorcontrib>Ozalp, Onur</creatorcontrib><creatorcontrib>Sarıboyacı, Ayla Eker</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Acta ophthalmologica (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atalay, Eray</au><au>Eyubova, Sevinj</au><au>Soykan, Merve Nur</au><au>Tasa, Burcugül Altuğ</au><au>Ozalp, Onur</au><au>Sarıboyacı, Ayla Eker</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of genes and miRNAs associated with scarless healing in orbital adipose‐derived and limbal‐derived mesenchymal stem cells treated with vitamin C</atitle><jtitle>Acta ophthalmologica (Oxford, England)</jtitle><date>2024-01</date><risdate>2024</risdate><volume>102</volume><issue>S279</issue><epage>n/a</epage><issn>1755-375X</issn><eissn>1755-3768</eissn><abstract>Aims/Purpose: In this study we aimed to investigate miRNAs associated with scarless wound healing in limbal‐derived stomal‐mesenchymal stem cells (hLMSC) and orbital adipose tissue‐derived stem cells (hOA‐MSC) treated individually with vitamin C and exosomes obtained after vitamin C treatment.
Methods: Isolation and characterization of hLMSCs and hOA‐MSCs were performed. MTT assay was employed to determine the ideal non‐cytotoxic and proliferative concentration of vitamin C (vit C). The selected concentration was applied to the cell culture medium of hLMSCs and hOA‐MSCs (vit C‐treated) followed by procurement of secreted exosomes (vit C‐exo) and their characterization. These exosomes were then used to enrich cell culture media of naïve hLMSCs and hOA‐MSCs. miR29, miR107, miR155, and miR543 miRNAs and Transforming Growth Factor Beta 1 (TGFβ1), TGFβ3 were analysed by Real‐Time PCR in both vit C treated and vit C‐exo‐treated cells.
Results: Vit C‐exo and vit C‐treated hLMSCs demonstrated slight reductions in TGFβ1 and TGFβ3 expressions compared to controls. In contrast, a modest increase in TGFβ1 and TGFβ3 levels was noted in hOA‐MSCs after vit‐C and vit C‐exo treatment. After vit C‐exo application, expression of miR155 and miR107 increased in hLMSCs and decreased in hOA‐MSCs. An opposite trend was observed with miR543 expression with an increase in hOA‐MSCs and a decrease in hLMSCs. miR29 associated with fibrosis was dramatically increased in hOA‐MSCs, but not in hLMSCs.
Conclusions: In conclusion, we show that miRNA and RNA contents of treated mesenchymal stem cells may vary depending on the origin of tissue. These results suggest that vitamin C could promote a relatively more pronounced anti‐fibrotic character in hOA‐MSCs.
The authors acknowledge the support from Eskisehir Osmangazi University, Scientific Research Projects (ESOGU‐BAP Grant ID: TOA‐2022‐2458).</abstract><cop>Malden</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/aos.16067</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose tissue Ascorbic acid Cell culture Culture media Cytotoxicity Exosomes Fibrosis Mesenchymal stem cells MicroRNAs miRNA Stem cells Transforming growth factor-b1 Vitamin C Wound healing |
| title | Investigation of genes and miRNAs associated with scarless healing in orbital adipose‐derived and limbal‐derived mesenchymal stem cells treated with vitamin C |
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