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A rapid, sensitive and specific detection of aggregated α-Synuclein by a liposome-immobilized cantilever sensor
Parkinson's disease (PD) is characterized by dopaminergic cell loss and the formation of Lewy bodies, of which the main component is aggregated and fibrillized alpha-synuclein (aSyn). Recent studies suggested that ultra-trace amounts of aSyn aggregates are also present in biofluid specimens, an...
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Published in: | IEEE sensors journal 2023-06, Vol.23 (12), p.1-1 |
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description | Parkinson's disease (PD) is characterized by dopaminergic cell loss and the formation of Lewy bodies, of which the main component is aggregated and fibrillized alpha-synuclein (aSyn). Recent studies suggested that ultra-trace amounts of aSyn aggregates are also present in biofluid specimens, and they can serve as a biomarker for PD. Because aSyn has been shown to possess a prion-like property, we attempted to enhance the sensitivity and specificity of a cantilever microsensor to detect aSyn aggregates by exploiting the properties of self-templating assembly and lipid interaction on the surface of liposome-immobilized cantilever sensor. We found that the liposome-immobilized cantilever sensor was able to successfully detect aSyn fibrils at a very low concentration (100 pg/ml), and the addition of aSyn monomers, which were converted into fibrils in the presence of aSyn aggregates and further acted as a template for fibrillization, lowered the detection limit to 10 pg/ml. The sensitivity of this cantilever sensor was comparable to or slightly superior to that of enzyme-linked immunosorbent assay (ELISA). Moreover, the lag time for the detection of aSyn fibrils has been significantly reduced to 100-120 minutes, compared to the tens of hours needed in conventional ELISA, Real-Time Quaking-Induced Conversion (RT-QuIC) and Protein Misfolding Cyclic Amplification (PMCA) assays. Finally, preliminary measurements of aSyn aggregates showed the possibilities of discriminating serum from PD and non-PD patients. The liposome-immobilized cantilever sensor could serve as a promising tool for the early or preclinical diagnosis of PD. |
doi_str_mv | 10.1109/JSEN.2023.3272659 |
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Recent studies suggested that ultra-trace amounts of aSyn aggregates are also present in biofluid specimens, and they can serve as a biomarker for PD. Because aSyn has been shown to possess a prion-like property, we attempted to enhance the sensitivity and specificity of a cantilever microsensor to detect aSyn aggregates by exploiting the properties of self-templating assembly and lipid interaction on the surface of liposome-immobilized cantilever sensor. We found that the liposome-immobilized cantilever sensor was able to successfully detect aSyn fibrils at a very low concentration (100 pg/ml), and the addition of aSyn monomers, which were converted into fibrils in the presence of aSyn aggregates and further acted as a template for fibrillization, lowered the detection limit to 10 pg/ml. The sensitivity of this cantilever sensor was comparable to or slightly superior to that of enzyme-linked immunosorbent assay (ELISA). Moreover, the lag time for the detection of aSyn fibrils has been significantly reduced to 100-120 minutes, compared to the tens of hours needed in conventional ELISA, Real-Time Quaking-Induced Conversion (RT-QuIC) and Protein Misfolding Cyclic Amplification (PMCA) assays. Finally, preliminary measurements of aSyn aggregates showed the possibilities of discriminating serum from PD and non-PD patients. The liposome-immobilized cantilever sensor could serve as a promising tool for the early or preclinical diagnosis of PD.</description><identifier>ISSN: 1530-437X</identifier><identifier>EISSN: 1558-1748</identifier><identifier>DOI: 10.1109/JSEN.2023.3272659</identifier><identifier>CODEN: ISJEAZ</identifier><language>eng</language><publisher>New York: IEEE</publisher><subject>Aggregates ; Biomarkers ; cantilever sensor ; Diseases ; Lag time ; Lipids ; liposome ; Liposomes ; Parkinson disease ; Parkinson's disease ; prion-lipid interaction ; Proteins ; self-templating assembly ; Sensitivity enhancement ; Sensor phenomena and characterization ; Sensors ; Surface stress ; Temperature sensors ; α-synuclein</subject><ispartof>IEEE sensors journal, 2023-06, Vol.23 (12), p.1-1</ispartof><rights>Copyright The Institute of Electrical and Electronics Engineers, Inc. (IEEE) 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-f211db8ed2de68ff2b8e1948c07e52035523080d8d9ff066af77ed903b5e1f7b3</citedby><cites>FETCH-LOGICAL-c337t-f211db8ed2de68ff2b8e1948c07e52035523080d8d9ff066af77ed903b5e1f7b3</cites><orcidid>0000-0002-1142-2669 ; 0000-0002-9613-6230 ; 0000-0002-9780-996X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://ieeexplore.ieee.org/document/10121603$$EHTML$$P50$$Gieee$$Hfree_for_read</linktohtml><link.rule.ids>315,786,790,27957,27958,55147</link.rule.ids></links><search><creatorcontrib>Kobayashi, Ryoko</creatorcontrib><creatorcontrib>Kamitani, Kotaro</creatorcontrib><creatorcontrib>Sawamura, Masanori</creatorcontrib><creatorcontrib>Yamakado, Hodaka</creatorcontrib><creatorcontrib>Takahashi, Ryosuke</creatorcontrib><creatorcontrib>Sohgawa, Masayuki</creatorcontrib><creatorcontrib>Noda, Minoru</creatorcontrib><title>A rapid, sensitive and specific detection of aggregated α-Synuclein by a liposome-immobilized cantilever sensor</title><title>IEEE sensors journal</title><addtitle>JSEN</addtitle><description>Parkinson's disease (PD) is characterized by dopaminergic cell loss and the formation of Lewy bodies, of which the main component is aggregated and fibrillized alpha-synuclein (aSyn). Recent studies suggested that ultra-trace amounts of aSyn aggregates are also present in biofluid specimens, and they can serve as a biomarker for PD. Because aSyn has been shown to possess a prion-like property, we attempted to enhance the sensitivity and specificity of a cantilever microsensor to detect aSyn aggregates by exploiting the properties of self-templating assembly and lipid interaction on the surface of liposome-immobilized cantilever sensor. We found that the liposome-immobilized cantilever sensor was able to successfully detect aSyn fibrils at a very low concentration (100 pg/ml), and the addition of aSyn monomers, which were converted into fibrils in the presence of aSyn aggregates and further acted as a template for fibrillization, lowered the detection limit to 10 pg/ml. The sensitivity of this cantilever sensor was comparable to or slightly superior to that of enzyme-linked immunosorbent assay (ELISA). Moreover, the lag time for the detection of aSyn fibrils has been significantly reduced to 100-120 minutes, compared to the tens of hours needed in conventional ELISA, Real-Time Quaking-Induced Conversion (RT-QuIC) and Protein Misfolding Cyclic Amplification (PMCA) assays. Finally, preliminary measurements of aSyn aggregates showed the possibilities of discriminating serum from PD and non-PD patients. The liposome-immobilized cantilever sensor could serve as a promising tool for the early or preclinical diagnosis of PD.</description><subject>Aggregates</subject><subject>Biomarkers</subject><subject>cantilever sensor</subject><subject>Diseases</subject><subject>Lag time</subject><subject>Lipids</subject><subject>liposome</subject><subject>Liposomes</subject><subject>Parkinson disease</subject><subject>Parkinson's disease</subject><subject>prion-lipid interaction</subject><subject>Proteins</subject><subject>self-templating assembly</subject><subject>Sensitivity enhancement</subject><subject>Sensor phenomena and characterization</subject><subject>Sensors</subject><subject>Surface stress</subject><subject>Temperature sensors</subject><subject>α-synuclein</subject><issn>1530-437X</issn><issn>1558-1748</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ESBDL</sourceid><recordid>eNpNkEtOwzAURSMEEqWwACQGlpiS4k8dO8OqKj9VMChIzCwnfq5cJXGw00plV2yENZFQBozeHZz7rnSS5JLgCSE4v31aLZ4nFFM2YVTQjOdHyYhwLlMipvJ4yAynUybeT5OzGDcYk1xwMUraGQq6deYGRWii69wOkG4Mii2UzroSGeig7JxvkLdIr9cB1roDg76_0tW-2ZYVuAYVe6RR5VoffQ2pq2tfuMp99lipm85VsIPwO-DDeXJidRXh4u-Ok7e7xev8IV2-3D_OZ8u0ZEx0qaWEmEKCoQYyaS3tM8mnssQCOMWMc8qwxEaa3FqcZdoKASbHrOBArCjYOLk-_G2D_9hC7NTGb0PTTyoqKc8w7QX0FDlQZfAxBrCqDa7WYa8IVoNYNYhVg1j1J7bvXB06DgD-8YSSDDP2A2c3dug</recordid><startdate>20230615</startdate><enddate>20230615</enddate><creator>Kobayashi, Ryoko</creator><creator>Kamitani, Kotaro</creator><creator>Sawamura, Masanori</creator><creator>Yamakado, Hodaka</creator><creator>Takahashi, Ryosuke</creator><creator>Sohgawa, Masayuki</creator><creator>Noda, Minoru</creator><general>IEEE</general><general>The Institute of Electrical and Electronics Engineers, Inc. (IEEE)</general><scope>97E</scope><scope>ESBDL</scope><scope>RIA</scope><scope>RIE</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-1142-2669</orcidid><orcidid>https://orcid.org/0000-0002-9613-6230</orcidid><orcidid>https://orcid.org/0000-0002-9780-996X</orcidid></search><sort><creationdate>20230615</creationdate><title>A rapid, sensitive and specific detection of aggregated α-Synuclein by a liposome-immobilized cantilever sensor</title><author>Kobayashi, Ryoko ; Kamitani, Kotaro ; Sawamura, Masanori ; Yamakado, Hodaka ; Takahashi, Ryosuke ; Sohgawa, Masayuki ; Noda, Minoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-f211db8ed2de68ff2b8e1948c07e52035523080d8d9ff066af77ed903b5e1f7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aggregates</topic><topic>Biomarkers</topic><topic>cantilever sensor</topic><topic>Diseases</topic><topic>Lag time</topic><topic>Lipids</topic><topic>liposome</topic><topic>Liposomes</topic><topic>Parkinson disease</topic><topic>Parkinson's disease</topic><topic>prion-lipid interaction</topic><topic>Proteins</topic><topic>self-templating assembly</topic><topic>Sensitivity enhancement</topic><topic>Sensor phenomena and characterization</topic><topic>Sensors</topic><topic>Surface stress</topic><topic>Temperature sensors</topic><topic>α-synuclein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Ryoko</creatorcontrib><creatorcontrib>Kamitani, Kotaro</creatorcontrib><creatorcontrib>Sawamura, Masanori</creatorcontrib><creatorcontrib>Yamakado, Hodaka</creatorcontrib><creatorcontrib>Takahashi, Ryosuke</creatorcontrib><creatorcontrib>Sohgawa, Masayuki</creatorcontrib><creatorcontrib>Noda, Minoru</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE Open Access Journals</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998–Present</collection><collection>IEEE Xplore</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>IEEE sensors journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Ryoko</au><au>Kamitani, Kotaro</au><au>Sawamura, Masanori</au><au>Yamakado, Hodaka</au><au>Takahashi, Ryosuke</au><au>Sohgawa, Masayuki</au><au>Noda, Minoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid, sensitive and specific detection of aggregated α-Synuclein by a liposome-immobilized cantilever sensor</atitle><jtitle>IEEE sensors journal</jtitle><stitle>JSEN</stitle><date>2023-06-15</date><risdate>2023</risdate><volume>23</volume><issue>12</issue><spage>1</spage><epage>1</epage><pages>1-1</pages><issn>1530-437X</issn><eissn>1558-1748</eissn><coden>ISJEAZ</coden><abstract>Parkinson's disease (PD) is characterized by dopaminergic cell loss and the formation of Lewy bodies, of which the main component is aggregated and fibrillized alpha-synuclein (aSyn). Recent studies suggested that ultra-trace amounts of aSyn aggregates are also present in biofluid specimens, and they can serve as a biomarker for PD. Because aSyn has been shown to possess a prion-like property, we attempted to enhance the sensitivity and specificity of a cantilever microsensor to detect aSyn aggregates by exploiting the properties of self-templating assembly and lipid interaction on the surface of liposome-immobilized cantilever sensor. We found that the liposome-immobilized cantilever sensor was able to successfully detect aSyn fibrils at a very low concentration (100 pg/ml), and the addition of aSyn monomers, which were converted into fibrils in the presence of aSyn aggregates and further acted as a template for fibrillization, lowered the detection limit to 10 pg/ml. The sensitivity of this cantilever sensor was comparable to or slightly superior to that of enzyme-linked immunosorbent assay (ELISA). Moreover, the lag time for the detection of aSyn fibrils has been significantly reduced to 100-120 minutes, compared to the tens of hours needed in conventional ELISA, Real-Time Quaking-Induced Conversion (RT-QuIC) and Protein Misfolding Cyclic Amplification (PMCA) assays. Finally, preliminary measurements of aSyn aggregates showed the possibilities of discriminating serum from PD and non-PD patients. The liposome-immobilized cantilever sensor could serve as a promising tool for the early or preclinical diagnosis of PD.</abstract><cop>New York</cop><pub>IEEE</pub><doi>10.1109/JSEN.2023.3272659</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1142-2669</orcidid><orcidid>https://orcid.org/0000-0002-9613-6230</orcidid><orcidid>https://orcid.org/0000-0002-9780-996X</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Aggregates Biomarkers cantilever sensor Diseases Lag time Lipids liposome Liposomes Parkinson disease Parkinson's disease prion-lipid interaction Proteins self-templating assembly Sensitivity enhancement Sensor phenomena and characterization Sensors Surface stress Temperature sensors α-synuclein |
title | A rapid, sensitive and specific detection of aggregated α-Synuclein by a liposome-immobilized cantilever sensor |
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