Nanobodies: their potential for applications in biotechnology, diagnosis and antiviral properties in Africa; focus on application in agriculture

Antibodies are key elements in routine immunological tests, having a long history in detection of plant pathogens due to their specificity, reliability and fast performance. Heavy chain-only antibodies (HCAbs), circulating in the blood of camels are IgG isotypes that lack the light (L) chains and th...

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Published in:Biotechnology, biotechnological equipment biotechnological equipment, 2021-01, Vol.35 (1), p.1331-1342
Main Authors: Njeru, Faith Njeri, Kusolwa, Paul Mbogo
Format: Article
Language:English
Subjects:
Agricultural research
Antibodies
Antigens
Antiviral activity
Aqueous solutions
Binders
Biotechnology
Blood circulation
camel
Chains
Cytosol
Domains
Engineers
Immunoglobulin G
Immunology
Isotypes
Nanobodies
Nanobody
Pathogens
Phage display
Phages
plant pathogen
Plant reliability
Plant resistance
resistance
single domain antibody
VHH
Yeasts
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Kusolwa, Paul Mbogo
description Antibodies are key elements in routine immunological tests, having a long history in detection of plant pathogens due to their specificity, reliability and fast performance. Heavy chain-only antibodies (HCAbs), circulating in the blood of camels are IgG isotypes that lack the light (L) chains and the heavy (H) chain is depleted of the first constant domain (CH1). The HCAbs interact with the antigen by virtue of only one single variable domain, referred to as VHH or nanobody (Nb). Owing to their unique properties: nanometer scale size, robust structure, stable and soluble behaviour in aqueous solution, high affinity and specificity for only one cognate target, as well as a sustainable source, Nbs are an ideal research tool in plants. There are several rapid and robust techniques for isolating highly specific Nbs. Antigen-specific Nbs are selected from constructed nanobody libraries (immune, naïve, synthetic) and specific binders are retrieved by phage display, yeast display or ribosome display. Their small size, easy folding in monomeric units and the possibility to express Nbs in the cytosol, make them attractive for their application to engineer resistance against plant pathogens, and the effect of multiple Nbs combined by gene stacking to engineer plant resistance can be studied. We summarize the current methodologies for isolation and production of Nbs, their application in detection of plant pathogens and nanobody-mediated resistance against plant pathogens. In addition, we explore novel implementations of VHHs in agricultural research. Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1974943 .
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source Taylor & Francis Open Access
subjects Agricultural research
Antibodies
Antigens
Antiviral activity
Aqueous solutions
Binders
Biotechnology
Blood circulation
camel
Chains
Cytosol
Domains
Engineers
Immunoglobulin G
Immunology
Isotypes
Nanobodies
Nanobody
Pathogens
Phage display
Phages
plant pathogen
Plant reliability
Plant resistance
resistance
single domain antibody
VHH
Yeasts
title Nanobodies: their potential for applications in biotechnology, diagnosis and antiviral properties in Africa; focus on application in agriculture
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