A Method for Quantitative Characterization of Fixed Histological Samples by Means of Digital Holographic Microscopy

Methods for quantitative characterization of fixed histological samples have been developed. These methods are based on a statistical analysis of phase images obtained by digital holographic microscopy. The suggested approach enables a fully automated processing of reconstructed phase images and mak...

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Bibliographic Details
Published in:Technical physics letters 2021-06, Vol.47 (6), p.428-431
Main Authors: Belashov, A. V., Zhikhoreva, A. A.
Format: Article
Language:English
Subjects:
Cartilage
Classical and Continuum Physics
Digital imaging
Holography
Image reconstruction
Microscopy
Optical properties
Physics
Physics and Astronomy
Statistical analysis
Statistical methods
Structural members
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Summary:Methods for quantitative characterization of fixed histological samples have been developed. These methods are based on a statistical analysis of phase images obtained by digital holographic microscopy. The suggested approach enables a fully automated processing of reconstructed phase images and makes it possible to obtain quantitative information about the optical characteristics and sizes of structural elements of a tissue in a histological sample. The method was tested on three types of histological sample: ciliated cylindrical epithelium, elastic cartilage, and liver.
ISSN:1063-7850
1090-6533
DOI:10.1134/S1063785021050035