The application of immuno-enzymal analysis as a method of early express diagnostics of rednoya in pond fish

Abstract Due to the lack of effective means of specific prevention of rubella in pond fish (CRF), it is obvious that early diagnosis is one of the most important and effective ways to combat this disease. This theoretical study is devoted to the development of an express method for the rapid detecti...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2021-03, Vol.723 (2), p.22101
Main Authors: Kapitova, I A, Spiridonov, A V, Glebova, I A, Gorbunov, A V
Format: Article
Language:English
Subjects:
Aeromonas punctata
Antibodies
Diagnosis
Enzyme-linked immunosorbent assay
Enzymes
Fish
Indicators
Ponds
Pseudomonas
Rubella
Sample preparation
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Summary:Abstract Due to the lack of effective means of specific prevention of rubella in pond fish (CRF), it is obvious that early diagnosis is one of the most important and effective ways to combat this disease. This theoretical study is devoted to the development of an express method for the rapid detection of antibodies to rubella pathogens in pond fish (Aeromonas punctata, Pseudomonas, Rabdovirus cyprini). To date, the diagnosis of CRC (Rabdovirus cyprini) is determined by the results of the clinical picture, taking into account anatomical data, epizootological indicators and virological research results. The diagnosis of CRC (Aeromonas punctata, Pseudomonas) is determined by bacteriological results, taking into account epizootological indicators, clinical signs and pathological changes. The use of enzyme-linked immunosorbent assay (ELISA) for the determination of CRC antibodies is a comprehensive diagnostic kit that allows you to significantly reduce the time for examination (sample preparation, reaction, results recording), and also reduce the number of errors caused by the human factor. The ELISA reaction makes it possible to quickly and efficiently obtain research results. As a result of the study, indicators of the effectiveness of the application of this technique for the diagnosis of CRC were proposed. The advantage of ELISA in comparison with the bacteriological method lies in the simplicity of setting and the reduction in the time of analysis (within 1-2 hours). The proposed method for the determination of rubella in pond fish using enzyme-linked immunosorbent assay allows, within the framework of laboratory monitoring, to carry out an express diagnosis of the CRC disease. The possibility of early diagnosis of this disease, the low cost of diagnostic kits and the ability to automate most stages of the reaction, demonstrate the practical value of the presented method.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/723/2/022101