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Ethylene mediates CuO NP-induced ultrastructural changes and oxidative stress in Arabidopsis thaliana leaves
Metallic oxide nanoparticles (NPs) have received great importance due to their unique properties and valuable applications in various fields. The release of nanoparticles into the environment at a large scale has become a serious threat to the ecosystem especially plants. Various reports have illust...
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| Published in: | Environmental science. Nano 2020-03, Vol.7 (3), p.938-953 |
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| cites | cdi_FETCH-LOGICAL-c259t-cfab2988f7ae2955280ea5a428441786a4df6003423deea8d80f3f9d8bf09e203 |
| container_end_page | 953 |
| container_issue | 3 |
| container_start_page | 938 |
| container_title | Environmental science. Nano |
| container_volume | 7 |
| creator | Azhar, Wardah Khan, Ali Raza Muhammad, Noor Liu, Bohan Song, Ge Hussain, Akhlaq Yasin, Muhammad Umair Khan, Sulaiman Munir, Raheel Gan, Yinbo |
| description | Metallic oxide nanoparticles (NPs) have received great importance due to their unique properties and valuable applications in various fields. The release of nanoparticles into the environment at a large scale has become a serious threat to the ecosystem especially plants. Various reports have illustrated the toxicity of CuO NPs to plants, but the hypothesis underlying ethylene-mediated CuO NP toxicity is still unknown. The present study was conducted to investigate the response of ethylene-insensitive mutants (
ein2-1, ein4
and
etr1-3
) and WT under CuO NP exposure. The results of the present study showed that CuO NPs adversely affected the biomass, chlorophyll contents, guard cells, stomatal aperture and other cell organelles, with consequent decline in plant biomass. Moreover, CuO NPs significantly increased ROS accumulation and induced toxicity in the leaves of all four genotypes. However, ethylene-insensitive mutants markedly showed less sensitivity to this damage as compared to WT seedlings. More importantly, higher levels of CuO NPs significantly increased the activity of ethylene reporter
EBS::GUS
lines and the transcript level of ethylene signaling and biosynthesis genes. Furthermore, the expression levels of antioxidant enzyme related-genes were slightly enhanced by the exogenous application of ethylene biosynthesis and perception antagonists cobalt (Co
2+
) and silver (Ag
+
), respectively, than the treatment with CuO NP stress alone. In contrast, it is significantly suppressed by an ethylene biosynthesis precursor (ACC), which further provided evidence that ethylene participates in CuO NP-induced oxidative damage and growth reduction in
Arabidopsis thaliana
leaves. |
| doi_str_mv | 10.1039/C9EN01302D |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2379685409</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2379685409</sourcerecordid><originalsourceid>FETCH-LOGICAL-c259t-cfab2988f7ae2955280ea5a428441786a4df6003423deea8d80f3f9d8bf09e203</originalsourceid><addsrcrecordid>eNpFkM1KAzEYRYMoWGo3PkHAnTCan8lMsiy1_kBpXeh6-Dr5YlOmMzXJFPv2jlR0de_icC8cQq45u-NMmvuZmS8Zl0w8nJGRYIpnmhf8_K8reUkmMW4ZY5wLJYtyRJp52hwbbJHu0HpIGOmsX9Hla-Zb29doad-kADGFvk59gIbWG2g_BgxaS7svbyH5A9IBwBipb-k0wNrbbh99pGkDjYcWaINwwHhFLhw0ESe_OSbvj_O32XO2WD29zKaLrBbKpKx2sBZGa1cCCqOU0AxBQS50nvNSF5BbVzAmcyEtImirmZPOWL12zKBgckxuTrv70H32GFO17frQDpeVkKUptMqZGajbE1WHLsaArtoHv4NwrDirfoRW_0LlN6a1aPY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2379685409</pqid></control><display><type>article</type><title>Ethylene mediates CuO NP-induced ultrastructural changes and oxidative stress in Arabidopsis thaliana leaves</title><source>Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)</source><creator>Azhar, Wardah ; Khan, Ali Raza ; Muhammad, Noor ; Liu, Bohan ; Song, Ge ; Hussain, Akhlaq ; Yasin, Muhammad Umair ; Khan, Sulaiman ; Munir, Raheel ; Gan, Yinbo</creator><creatorcontrib>Azhar, Wardah ; Khan, Ali Raza ; Muhammad, Noor ; Liu, Bohan ; Song, Ge ; Hussain, Akhlaq ; Yasin, Muhammad Umair ; Khan, Sulaiman ; Munir, Raheel ; Gan, Yinbo</creatorcontrib><description>Metallic oxide nanoparticles (NPs) have received great importance due to their unique properties and valuable applications in various fields. The release of nanoparticles into the environment at a large scale has become a serious threat to the ecosystem especially plants. Various reports have illustrated the toxicity of CuO NPs to plants, but the hypothesis underlying ethylene-mediated CuO NP toxicity is still unknown. The present study was conducted to investigate the response of ethylene-insensitive mutants (
ein2-1, ein4
and
etr1-3
) and WT under CuO NP exposure. The results of the present study showed that CuO NPs adversely affected the biomass, chlorophyll contents, guard cells, stomatal aperture and other cell organelles, with consequent decline in plant biomass. Moreover, CuO NPs significantly increased ROS accumulation and induced toxicity in the leaves of all four genotypes. However, ethylene-insensitive mutants markedly showed less sensitivity to this damage as compared to WT seedlings. More importantly, higher levels of CuO NPs significantly increased the activity of ethylene reporter
EBS::GUS
lines and the transcript level of ethylene signaling and biosynthesis genes. Furthermore, the expression levels of antioxidant enzyme related-genes were slightly enhanced by the exogenous application of ethylene biosynthesis and perception antagonists cobalt (Co
2+
) and silver (Ag
+
), respectively, than the treatment with CuO NP stress alone. In contrast, it is significantly suppressed by an ethylene biosynthesis precursor (ACC), which further provided evidence that ethylene participates in CuO NP-induced oxidative damage and growth reduction in
Arabidopsis thaliana
leaves.</description><identifier>ISSN: 2051-8153</identifier><identifier>EISSN: 2051-8161</identifier><identifier>DOI: 10.1039/C9EN01302D</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Antioxidants ; Apertures ; Arabidopsis thaliana ; Biological stress ; Biomass ; Biosynthesis ; Carbon dioxide ; Chlorophyll ; Chlorophylls ; Cobalt ; Copper oxides ; Cytoplasmic organelles ; Damage ; Environmental changes ; Ethene ; Ethylene ; Gene expression ; Genes ; Genotypes ; Guard cells ; Leaves ; Levels ; Mutants ; Nanoparticles ; Organelles ; Oxidative stress ; Plant biomass ; Seedlings ; Silver ; Stomata ; Toxicity ; Transcription</subject><ispartof>Environmental science. Nano, 2020-03, Vol.7 (3), p.938-953</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c259t-cfab2988f7ae2955280ea5a428441786a4df6003423deea8d80f3f9d8bf09e203</citedby><cites>FETCH-LOGICAL-c259t-cfab2988f7ae2955280ea5a428441786a4df6003423deea8d80f3f9d8bf09e203</cites><orcidid>0000-0001-5973-8702</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Azhar, Wardah</creatorcontrib><creatorcontrib>Khan, Ali Raza</creatorcontrib><creatorcontrib>Muhammad, Noor</creatorcontrib><creatorcontrib>Liu, Bohan</creatorcontrib><creatorcontrib>Song, Ge</creatorcontrib><creatorcontrib>Hussain, Akhlaq</creatorcontrib><creatorcontrib>Yasin, Muhammad Umair</creatorcontrib><creatorcontrib>Khan, Sulaiman</creatorcontrib><creatorcontrib>Munir, Raheel</creatorcontrib><creatorcontrib>Gan, Yinbo</creatorcontrib><title>Ethylene mediates CuO NP-induced ultrastructural changes and oxidative stress in Arabidopsis thaliana leaves</title><title>Environmental science. Nano</title><description>Metallic oxide nanoparticles (NPs) have received great importance due to their unique properties and valuable applications in various fields. The release of nanoparticles into the environment at a large scale has become a serious threat to the ecosystem especially plants. Various reports have illustrated the toxicity of CuO NPs to plants, but the hypothesis underlying ethylene-mediated CuO NP toxicity is still unknown. The present study was conducted to investigate the response of ethylene-insensitive mutants (
ein2-1, ein4
and
etr1-3
) and WT under CuO NP exposure. The results of the present study showed that CuO NPs adversely affected the biomass, chlorophyll contents, guard cells, stomatal aperture and other cell organelles, with consequent decline in plant biomass. Moreover, CuO NPs significantly increased ROS accumulation and induced toxicity in the leaves of all four genotypes. However, ethylene-insensitive mutants markedly showed less sensitivity to this damage as compared to WT seedlings. More importantly, higher levels of CuO NPs significantly increased the activity of ethylene reporter
EBS::GUS
lines and the transcript level of ethylene signaling and biosynthesis genes. Furthermore, the expression levels of antioxidant enzyme related-genes were slightly enhanced by the exogenous application of ethylene biosynthesis and perception antagonists cobalt (Co
2+
) and silver (Ag
+
), respectively, than the treatment with CuO NP stress alone. In contrast, it is significantly suppressed by an ethylene biosynthesis precursor (ACC), which further provided evidence that ethylene participates in CuO NP-induced oxidative damage and growth reduction in
Arabidopsis thaliana
leaves.</description><subject>Antioxidants</subject><subject>Apertures</subject><subject>Arabidopsis thaliana</subject><subject>Biological stress</subject><subject>Biomass</subject><subject>Biosynthesis</subject><subject>Carbon dioxide</subject><subject>Chlorophyll</subject><subject>Chlorophylls</subject><subject>Cobalt</subject><subject>Copper oxides</subject><subject>Cytoplasmic organelles</subject><subject>Damage</subject><subject>Environmental changes</subject><subject>Ethene</subject><subject>Ethylene</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genotypes</subject><subject>Guard cells</subject><subject>Leaves</subject><subject>Levels</subject><subject>Mutants</subject><subject>Nanoparticles</subject><subject>Organelles</subject><subject>Oxidative stress</subject><subject>Plant biomass</subject><subject>Seedlings</subject><subject>Silver</subject><subject>Stomata</subject><subject>Toxicity</subject><subject>Transcription</subject><issn>2051-8153</issn><issn>2051-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpFkM1KAzEYRYMoWGo3PkHAnTCan8lMsiy1_kBpXeh6-Dr5YlOmMzXJFPv2jlR0de_icC8cQq45u-NMmvuZmS8Zl0w8nJGRYIpnmhf8_K8reUkmMW4ZY5wLJYtyRJp52hwbbJHu0HpIGOmsX9Hla-Zb29doad-kADGFvk59gIbWG2g_BgxaS7svbyH5A9IBwBipb-k0wNrbbh99pGkDjYcWaINwwHhFLhw0ESe_OSbvj_O32XO2WD29zKaLrBbKpKx2sBZGa1cCCqOU0AxBQS50nvNSF5BbVzAmcyEtImirmZPOWL12zKBgckxuTrv70H32GFO17frQDpeVkKUptMqZGajbE1WHLsaArtoHv4NwrDirfoRW_0LlN6a1aPY</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Azhar, Wardah</creator><creator>Khan, Ali Raza</creator><creator>Muhammad, Noor</creator><creator>Liu, Bohan</creator><creator>Song, Ge</creator><creator>Hussain, Akhlaq</creator><creator>Yasin, Muhammad Umair</creator><creator>Khan, Sulaiman</creator><creator>Munir, Raheel</creator><creator>Gan, Yinbo</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-5973-8702</orcidid></search><sort><creationdate>20200301</creationdate><title>Ethylene mediates CuO NP-induced ultrastructural changes and oxidative stress in Arabidopsis thaliana leaves</title><author>Azhar, Wardah ; Khan, Ali Raza ; Muhammad, Noor ; Liu, Bohan ; Song, Ge ; Hussain, Akhlaq ; Yasin, Muhammad Umair ; Khan, Sulaiman ; Munir, Raheel ; Gan, Yinbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259t-cfab2988f7ae2955280ea5a428441786a4df6003423deea8d80f3f9d8bf09e203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antioxidants</topic><topic>Apertures</topic><topic>Arabidopsis thaliana</topic><topic>Biological stress</topic><topic>Biomass</topic><topic>Biosynthesis</topic><topic>Carbon dioxide</topic><topic>Chlorophyll</topic><topic>Chlorophylls</topic><topic>Cobalt</topic><topic>Copper oxides</topic><topic>Cytoplasmic organelles</topic><topic>Damage</topic><topic>Environmental changes</topic><topic>Ethene</topic><topic>Ethylene</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genotypes</topic><topic>Guard cells</topic><topic>Leaves</topic><topic>Levels</topic><topic>Mutants</topic><topic>Nanoparticles</topic><topic>Organelles</topic><topic>Oxidative stress</topic><topic>Plant biomass</topic><topic>Seedlings</topic><topic>Silver</topic><topic>Stomata</topic><topic>Toxicity</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azhar, Wardah</creatorcontrib><creatorcontrib>Khan, Ali Raza</creatorcontrib><creatorcontrib>Muhammad, Noor</creatorcontrib><creatorcontrib>Liu, Bohan</creatorcontrib><creatorcontrib>Song, Ge</creatorcontrib><creatorcontrib>Hussain, Akhlaq</creatorcontrib><creatorcontrib>Yasin, Muhammad Umair</creatorcontrib><creatorcontrib>Khan, Sulaiman</creatorcontrib><creatorcontrib>Munir, Raheel</creatorcontrib><creatorcontrib>Gan, Yinbo</creatorcontrib><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Environmental science. Nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azhar, Wardah</au><au>Khan, Ali Raza</au><au>Muhammad, Noor</au><au>Liu, Bohan</au><au>Song, Ge</au><au>Hussain, Akhlaq</au><au>Yasin, Muhammad Umair</au><au>Khan, Sulaiman</au><au>Munir, Raheel</au><au>Gan, Yinbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ethylene mediates CuO NP-induced ultrastructural changes and oxidative stress in Arabidopsis thaliana leaves</atitle><jtitle>Environmental science. Nano</jtitle><date>2020-03-01</date><risdate>2020</risdate><volume>7</volume><issue>3</issue><spage>938</spage><epage>953</epage><pages>938-953</pages><issn>2051-8153</issn><eissn>2051-8161</eissn><abstract>Metallic oxide nanoparticles (NPs) have received great importance due to their unique properties and valuable applications in various fields. The release of nanoparticles into the environment at a large scale has become a serious threat to the ecosystem especially plants. Various reports have illustrated the toxicity of CuO NPs to plants, but the hypothesis underlying ethylene-mediated CuO NP toxicity is still unknown. The present study was conducted to investigate the response of ethylene-insensitive mutants (
ein2-1, ein4
and
etr1-3
) and WT under CuO NP exposure. The results of the present study showed that CuO NPs adversely affected the biomass, chlorophyll contents, guard cells, stomatal aperture and other cell organelles, with consequent decline in plant biomass. Moreover, CuO NPs significantly increased ROS accumulation and induced toxicity in the leaves of all four genotypes. However, ethylene-insensitive mutants markedly showed less sensitivity to this damage as compared to WT seedlings. More importantly, higher levels of CuO NPs significantly increased the activity of ethylene reporter
EBS::GUS
lines and the transcript level of ethylene signaling and biosynthesis genes. Furthermore, the expression levels of antioxidant enzyme related-genes were slightly enhanced by the exogenous application of ethylene biosynthesis and perception antagonists cobalt (Co
2+
) and silver (Ag
+
), respectively, than the treatment with CuO NP stress alone. In contrast, it is significantly suppressed by an ethylene biosynthesis precursor (ACC), which further provided evidence that ethylene participates in CuO NP-induced oxidative damage and growth reduction in
Arabidopsis thaliana
leaves.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/C9EN01302D</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-5973-8702</orcidid></addata></record> |
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| ispartof | Environmental science. Nano, 2020-03, Vol.7 (3), p.938-953 |
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| language | eng |
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| source | Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list) |
| subjects | Antioxidants Apertures Arabidopsis thaliana Biological stress Biomass Biosynthesis Carbon dioxide Chlorophyll Chlorophylls Cobalt Copper oxides Cytoplasmic organelles Damage Environmental changes Ethene Ethylene Gene expression Genes Genotypes Guard cells Leaves Levels Mutants Nanoparticles Organelles Oxidative stress Plant biomass Seedlings Silver Stomata Toxicity Transcription |
| title | Ethylene mediates CuO NP-induced ultrastructural changes and oxidative stress in Arabidopsis thaliana leaves |
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