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Determination of the Mycelium and Antigens of a Number of Micromycetes in Soil Extracts via Enzyme-Linked Immunosorbent Assay
Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the...
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| Published in: | Applied biochemistry and microbiology 2020, Vol.56 (1), p.72-77 |
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| Main Authors: | , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c353t-2516047b41fd7fb1361cf269895372dde5b34d8bac3948df030f5bc79c7cb1e13 |
| container_end_page | 77 |
| container_issue | 1 |
| container_start_page | 72 |
| container_title | Applied biochemistry and microbiology |
| container_volume | 56 |
| creator | Ivanova, A. E. Shutova, A. S. Gannesen, A. V. Lebedin, Y. S. Eremin, S. A. |
| description | Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the length of the growing mycelium) with the concentrations of antigens detected by the two-site sandwich ELISA method and the competitive ELISA method was evaluated. It has been shown that the methods are highly effective in the case of long incubation (more than 5 days) of soil samples and are ineffective for short-term incubation, because there was no correlation between hypha length and antigen concentration during the first day of cultivation. The perspectivity of ELISA methods for the detection of some soil microfungi from genera
Aspergillus
(
Asp. flavus, Asp. fumigatus, Asp. niger
),
Fusarium
(
F. solani
,
F. poae
), and others (
Alternaria alternata, Phoma lingham, Mucor hiemalis
) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA. |
| doi_str_mv | 10.1134/S0003683820010081 |
| format | article |
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Aspergillus
(
Asp. flavus, Asp. fumigatus, Asp. niger
),
Fusarium
(
F. solani
,
F. poae
), and others (
Alternaria alternata, Phoma lingham, Mucor hiemalis
) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683820010081</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Antigens ; Biochemistry ; Biomedical and Life Sciences ; Cultivation ; Enzyme-linked immunosorbent assay ; Enzymes ; Life Sciences ; Medical Microbiology ; Microbiology ; Opportunist infection ; Soils</subject><ispartof>Applied biochemistry and microbiology, 2020, Vol.56 (1), p.72-77</ispartof><rights>Pleiades Publishing, Inc. 2020</rights><rights>2020© Pleiades Publishing, Inc. 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-2516047b41fd7fb1361cf269895372dde5b34d8bac3948df030f5bc79c7cb1e13</citedby><cites>FETCH-LOGICAL-c353t-2516047b41fd7fb1361cf269895372dde5b34d8bac3948df030f5bc79c7cb1e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Ivanova, A. E.</creatorcontrib><creatorcontrib>Shutova, A. S.</creatorcontrib><creatorcontrib>Gannesen, A. V.</creatorcontrib><creatorcontrib>Lebedin, Y. S.</creatorcontrib><creatorcontrib>Eremin, S. A.</creatorcontrib><title>Determination of the Mycelium and Antigens of a Number of Micromycetes in Soil Extracts via Enzyme-Linked Immunosorbent Assay</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the length of the growing mycelium) with the concentrations of antigens detected by the two-site sandwich ELISA method and the competitive ELISA method was evaluated. It has been shown that the methods are highly effective in the case of long incubation (more than 5 days) of soil samples and are ineffective for short-term incubation, because there was no correlation between hypha length and antigen concentration during the first day of cultivation. The perspectivity of ELISA methods for the detection of some soil microfungi from genera
Aspergillus
(
Asp. flavus, Asp. fumigatus, Asp. niger
),
Fusarium
(
F. solani
,
F. poae
), and others (
Alternaria alternata, Phoma lingham, Mucor hiemalis
) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA.</description><subject>Antigens</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cultivation</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Opportunist infection</subject><subject>Soils</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1UMtOAyEUJUYTa_UD3JG4HoVhHrBsatUmrS6q6wnDQKUWUGCMNfHfZVITF8bVvTfncXMOAOcYXWJMiqsVQohUlNAcIYwQxQdghCtEM4Ly4hCMBjgb8GNwEsImnayibAS-rmWU3mjLo3YWOgXjs4TLnZBb3RvIbQcnNuq1tGEAObzvTSv9sC-18M4kZpQBagtXTm_h7CN6LmKA75rDmf3cGZkttH2RHZwb01sXnG-ljXASAt-dgiPFt0Ge_cwxeLqZPU7vssXD7Xw6WWSClCRmeZmiFHVbYNXVqsWkwkLlFaOsJHXedbJsSdHRlgvCCtopRJAqW1EzUYsWS0zG4GLv--rdWy9DbDau9za9bHJSV6ysS0YSC-9ZKVcIXqrm1WvD_a7BqBlabv60nDT5XhMS166l_3X-X_QN4VZ_Kg</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Ivanova, A. E.</creator><creator>Shutova, A. S.</creator><creator>Gannesen, A. V.</creator><creator>Lebedin, Y. S.</creator><creator>Eremin, S. A.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>2020</creationdate><title>Determination of the Mycelium and Antigens of a Number of Micromycetes in Soil Extracts via Enzyme-Linked Immunosorbent Assay</title><author>Ivanova, A. E. ; Shutova, A. S. ; Gannesen, A. V. ; Lebedin, Y. S. ; Eremin, S. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-2516047b41fd7fb1361cf269895372dde5b34d8bac3948df030f5bc79c7cb1e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antigens</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cultivation</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Opportunist infection</topic><topic>Soils</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ivanova, A. E.</creatorcontrib><creatorcontrib>Shutova, A. S.</creatorcontrib><creatorcontrib>Gannesen, A. V.</creatorcontrib><creatorcontrib>Lebedin, Y. S.</creatorcontrib><creatorcontrib>Eremin, S. A.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivanova, A. E.</au><au>Shutova, A. S.</au><au>Gannesen, A. V.</au><au>Lebedin, Y. S.</au><au>Eremin, S. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of the Mycelium and Antigens of a Number of Micromycetes in Soil Extracts via Enzyme-Linked Immunosorbent Assay</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2020</date><risdate>2020</risdate><volume>56</volume><issue>1</issue><spage>72</spage><epage>77</epage><pages>72-77</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Sixteen variants of test systems (two-site sandwich and competitive enzyme-linked immunosorbent assay (ELISA)) were studied to determine the concentration of microfungi, some of which are known opportunistic pathogens for humans, in soil extracts. The correlation of the amount of fungal biomass (the length of the growing mycelium) with the concentrations of antigens detected by the two-site sandwich ELISA method and the competitive ELISA method was evaluated. It has been shown that the methods are highly effective in the case of long incubation (more than 5 days) of soil samples and are ineffective for short-term incubation, because there was no correlation between hypha length and antigen concentration during the first day of cultivation. The perspectivity of ELISA methods for the detection of some soil microfungi from genera
Aspergillus
(
Asp. flavus, Asp. fumigatus, Asp. niger
),
Fusarium
(
F. solani
,
F. poae
), and others (
Alternaria alternata, Phoma lingham, Mucor hiemalis
) was shown. The competitive ELISA method was more efficient than two-site sandwich ELISA.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683820010081</doi><tpages>6</tpages></addata></record> |
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| subjects | Antigens Biochemistry Biomedical and Life Sciences Cultivation Enzyme-linked immunosorbent assay Enzymes Life Sciences Medical Microbiology Microbiology Opportunist infection Soils |
| title | Determination of the Mycelium and Antigens of a Number of Micromycetes in Soil Extracts via Enzyme-Linked Immunosorbent Assay |
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