A 3.3 Å‐Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of Its Thermal Stability

Respiratory chain complexes convert energy by coupling electron flow to transmembrane proton translocation. Owing to a lack of atomic structures of cytochrome bc1 complex (Complex III) from thermophilic bacteria, little is known about the adaptations of this macromolecular machine to hyperthermophil...

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Bibliographic Details
Published in:Angewandte Chemie 2020-01, Vol.132 (1), p.351-359
Main Authors: Zhu, Guoliang, Zeng, Hui, Zhang, Shuangbo, Juli, Jana, Pang, Xiaoyun, Hoffmann, Jan, Zhang, Yan, Morgner, Nina, Zhu, Yun, Peng, Guohong, Michel, Hartmut, Sun, Fei
Format: Article
Language:English
Subjects:
Adaptation
Bacteria
Chemistry
Cytochrom-bc1-Komplex
Cytochrome
Cytochrome bc1
Cytochromes
Electron transport
Enzymkatalyse
Flow stability
Hyperthermophile Spezies
Macromolecules
Phospholipids
Protein-Protein-Wechselwirkungen
Proteinstrukturen
Thermal environments
Thermal stability
Thermophilic bacteria
Translocation
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Summary:Respiratory chain complexes convert energy by coupling electron flow to transmembrane proton translocation. Owing to a lack of atomic structures of cytochrome bc1 complex (Complex III) from thermophilic bacteria, little is known about the adaptations of this macromolecular machine to hyperthermophilic environments. In this study, we purified the cytochrome bc1 complex of Aquifex aeolicus, one of the most extreme thermophilic bacteria known, and determined its structure with and without an inhibitor at 3.3 Å resolution. Several residues unique for thermophilic bacteria were detected that provide additional stabilization for the structure. An extra transmembrane helix at the N‐terminus of cyt. c1 was found to greatly enhance the interaction between cyt. b and cyt. c1, and to bind a phospholipid molecule to stabilize the complex in the membrane. These results provide the structural basis for the hyperstability of the cytochrome bc1 complex in an extreme thermal environment. Manche mögen's (sehr) heiß! Die Hyperstabilität des respiratorischen Komplexes III aus Aquifex aeolicus liefert eine geeignete Umgebung für den internen Elektronentransfer bei hoher Temperatur.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201911554