Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride

SO2-4 transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO2-4 (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO2-4 on both sides, net active SO secretion occurred (8.55 plus or minus 0.9...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2003-02, Vol.53 (2), p.R380-R388
Main Authors: PELIS, Ryan M, RENFRO, J. Larry
Format: Article
Language:English
Subjects:
Biological and medical sciences
Digestive system
Fish
Fundamental and applied biological sciences. Psychology
Ions
Metabolisms and neurohumoral controls
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Water and mineral metabolism. Osmoregulation. Acidobasic balance
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page R388
container_issue 2
container_start_page R380
container_title American journal of physiology. Regulatory, integrative and comparative physiology
container_volume 53
creator PELIS, Ryan M
RENFRO, J. Larry
description SO2-4 transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO2-4 (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO2-4 on both sides, net active SO secretion occurred (8.55 plus or minus 0.96 nmol . cm-2 . h-1). NaCN (10 mM), ouabain (10-4 M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl- and HCO-3 together (Cl--HCO-3) or Cl- alone blocked net secretion, whereas removal of luminal HCO-3 alone increased net secretion. SO2-4 uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl- gradient (in > out) and unaffected by a trans-HCO-3 gradient (in > out). Short-circuiting with K+ (in = out) and valinomycin had no effect on Cl--stimulated SO2-4 uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO2-4 absorption in marine teleosts and that active SO2-4 secretion is in exchange for luminal Cl-.
format article
fullrecord <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_journals_229720531</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>283105371</sourcerecordid><originalsourceid>FETCH-LOGICAL-p571-8ec48e1f7135ee275e5709d1cd274a548c72a5750d70cba76d73435536ec044e3</originalsourceid><addsrcrecordid>eNotj01LxDAYhIMoWFf_QxA8FvLZtMdl8QsWvOy9ZNM32yzZpCatuv_eiHuaGXgYZq5QRSVjNRUduUYV4Q2vG0q7W3SX85EQIrjgFYK1md0X4Lx4q-eiYBLMLga8P-N5BOzCDHl2AXC0-PsvJWx9XMJQjMuFSXE5jBh-zKjDAbCNCfvl5IL22Iw-JjfAPbqx2md4uOgK7V6ed5u3evvx-r5Zb-tJKlq3YEQL1CrKJQBTEqQi3UDNwJTQUrRGMS2VJIMiZq9VM6jyQUregCFCAF-hx__aKcXPpczuj3FJZUjuGesUI5LTAj1dIJ2N9jbpYFzup-ROOp17KhohO9HyX5N9YAc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>229720531</pqid></control><display><type>article</type><title>Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride</title><source>American Physiological Society Free</source><creator>PELIS, Ryan M ; RENFRO, J. Larry</creator><creatorcontrib>PELIS, Ryan M ; RENFRO, J. Larry</creatorcontrib><description>SO2-4 transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO2-4 (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO2-4 on both sides, net active SO secretion occurred (8.55 plus or minus 0.96 nmol . cm-2 . h-1). NaCN (10 mM), ouabain (10-4 M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl- and HCO-3 together (Cl--HCO-3) or Cl- alone blocked net secretion, whereas removal of luminal HCO-3 alone increased net secretion. SO2-4 uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl- gradient (in &gt; out) and unaffected by a trans-HCO-3 gradient (in &gt; out). Short-circuiting with K+ (in = out) and valinomycin had no effect on Cl--stimulated SO2-4 uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO2-4 absorption in marine teleosts and that active SO2-4 secretion is in exchange for luminal Cl-.</description><identifier>ISSN: 0363-6119</identifier><identifier>EISSN: 1522-1490</identifier><identifier>CODEN: AJPRDO</identifier><language>eng</language><publisher>Bethesda, MD: American Physiological Society</publisher><subject>Biological and medical sciences ; Digestive system ; Fish ; Fundamental and applied biological sciences. Psychology ; Ions ; Metabolisms and neurohumoral controls ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Water and mineral metabolism. Osmoregulation. Acidobasic balance</subject><ispartof>American journal of physiology. Regulatory, integrative and comparative physiology, 2003-02, Vol.53 (2), p.R380-R388</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright American Physiological Society Feb 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=14645948$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>PELIS, Ryan M</creatorcontrib><creatorcontrib>RENFRO, J. Larry</creatorcontrib><title>Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride</title><title>American journal of physiology. Regulatory, integrative and comparative physiology</title><description>SO2-4 transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO2-4 (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO2-4 on both sides, net active SO secretion occurred (8.55 plus or minus 0.96 nmol . cm-2 . h-1). NaCN (10 mM), ouabain (10-4 M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl- and HCO-3 together (Cl--HCO-3) or Cl- alone blocked net secretion, whereas removal of luminal HCO-3 alone increased net secretion. SO2-4 uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl- gradient (in &gt; out) and unaffected by a trans-HCO-3 gradient (in &gt; out). Short-circuiting with K+ (in = out) and valinomycin had no effect on Cl--stimulated SO2-4 uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO2-4 absorption in marine teleosts and that active SO2-4 secretion is in exchange for luminal Cl-.</description><subject>Biological and medical sciences</subject><subject>Digestive system</subject><subject>Fish</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ions</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Water and mineral metabolism. Osmoregulation. Acidobasic balance</subject><issn>0363-6119</issn><issn>1522-1490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNotj01LxDAYhIMoWFf_QxA8FvLZtMdl8QsWvOy9ZNM32yzZpCatuv_eiHuaGXgYZq5QRSVjNRUduUYV4Q2vG0q7W3SX85EQIrjgFYK1md0X4Lx4q-eiYBLMLga8P-N5BOzCDHl2AXC0-PsvJWx9XMJQjMuFSXE5jBh-zKjDAbCNCfvl5IL22Iw-JjfAPbqx2md4uOgK7V6ed5u3evvx-r5Zb-tJKlq3YEQL1CrKJQBTEqQi3UDNwJTQUrRGMS2VJIMiZq9VM6jyQUregCFCAF-hx__aKcXPpczuj3FJZUjuGesUI5LTAj1dIJ2N9jbpYFzup-ROOp17KhohO9HyX5N9YAc</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>PELIS, Ryan M</creator><creator>RENFRO, J. Larry</creator><general>American Physiological Society</general><scope>IQODW</scope><scope>7QP</scope><scope>7QR</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20030201</creationdate><title>Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride</title><author>PELIS, Ryan M ; RENFRO, J. Larry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p571-8ec48e1f7135ee275e5709d1cd274a548c72a5750d70cba76d73435536ec044e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Digestive system</topic><topic>Fish</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ions</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Water and mineral metabolism. Osmoregulation. Acidobasic balance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PELIS, Ryan M</creatorcontrib><creatorcontrib>RENFRO, J. Larry</creatorcontrib><collection>Pascal-Francis</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PELIS, Ryan M</au><au>RENFRO, J. Larry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride</atitle><jtitle>American journal of physiology. Regulatory, integrative and comparative physiology</jtitle><date>2003-02-01</date><risdate>2003</risdate><volume>53</volume><issue>2</issue><spage>R380</spage><epage>R388</epage><pages>R380-R388</pages><issn>0363-6119</issn><eissn>1522-1490</eissn><coden>AJPRDO</coden><abstract>SO2-4 transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO2-4 (physiological level) bathing the lumen, net absorption (lumen to blood) dominated. Under short-circuited conditions, 1 mM SO2-4 on both sides, net active SO secretion occurred (8.55 plus or minus 0.96 nmol . cm-2 . h-1). NaCN (10 mM), ouabain (10-4 M), and luminal DIDS (0.2 mM) inhibited net secretion. Removal of luminal Cl- and HCO-3 together (Cl--HCO-3) or Cl- alone blocked net secretion, whereas removal of luminal HCO-3 alone increased net secretion. SO2-4 uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl- gradient (in &gt; out) and unaffected by a trans-HCO-3 gradient (in &gt; out). Short-circuiting with K+ (in = out) and valinomycin had no effect on Cl--stimulated SO2-4 uptake, suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. It was concluded that the intestine is a site of SO2-4 absorption in marine teleosts and that active SO2-4 secretion is in exchange for luminal Cl-.</abstract><cop>Bethesda, MD</cop><pub>American Physiological Society</pub></addata></record>
fulltext fulltext
identifier ISSN: 0363-6119
ispartof American journal of physiology. Regulatory, integrative and comparative physiology, 2003-02, Vol.53 (2), p.R380-R388
issn 0363-6119
1522-1490
language eng
recordid cdi_proquest_journals_229720531
source American Physiological Society Free
subjects Biological and medical sciences
Digestive system
Fish
Fundamental and applied biological sciences. Psychology
Ions
Metabolisms and neurohumoral controls
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Water and mineral metabolism. Osmoregulation. Acidobasic balance
title Active sulfate secretion by the intestine of winter flounder is through exchange for luminal chloride
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T17%3A28%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Active%20sulfate%20secretion%20by%20the%20intestine%20of%20winter%20flounder%20is%20through%20exchange%20for%20luminal%20chloride&rft.jtitle=American%20journal%20of%20physiology.%20Regulatory,%20integrative%20and%20comparative%20physiology&rft.au=PELIS,%20Ryan%20M&rft.date=2003-02-01&rft.volume=53&rft.issue=2&rft.spage=R380&rft.epage=R388&rft.pages=R380-R388&rft.issn=0363-6119&rft.eissn=1522-1490&rft.coden=AJPRDO&rft_id=info:doi/&rft_dat=%3Cproquest_pasca%3E283105371%3C/proquest_pasca%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p571-8ec48e1f7135ee275e5709d1cd274a548c72a5750d70cba76d73435536ec044e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=229720531&rft_id=info:pmid/&rfr_iscdi=true