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5‐ALA/SFC Attenuated Binge Alcohol–Induced Gut Leakiness and Inflammatory Liver Disease in HIV Transgenic Rats
Background This study aimed to investigate the protective effect of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) against binge alcohol–induced gut leakiness and inflammatory liver disease in HIV transgenic (TG) rats. Methods TG rats were treated with 3 consecutive doses of binge et...
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| Published in: | Alcoholism, clinical and experimental research clinical and experimental research, 2019-08, Vol.43 (8), p.1651-1661 |
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| container_end_page | 1661 |
| container_issue | 8 |
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| container_title | Alcoholism, clinical and experimental research |
| container_volume | 43 |
| creator | Liu, Chi Zhu, Ping Fujino, Masayuki Zhu, Shuoji Ito, Hidenori Takahashi, Kiwamu Nakajima, Motowo Tanaka, Tohru Zhuang, Jian Li, Xiao‐Kang |
| description | Background
This study aimed to investigate the protective effect of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) against binge alcohol–induced gut leakiness and inflammatory liver disease in HIV transgenic (TG) rats.
Methods
TG rats were treated with 3 consecutive doses of binge ethanol (EtOH) with or without 5‐ALA/SFC. Blood and liver tissue samples were collected at 6 hours following the last dose of EtOH.
Results
Compared with the wild‐type (WT) rats, the TG rats showed increased sensitivity to alcohol‐mediated inflammation, as evidenced by the significantly elevated levels of serum endotoxin, AST, ALT, ED1, and ED2 staining in liver. In contrast, 5‐ALA/SFC improved the above biochemical and histochemical profiles. 5‐ALA/SFC also attenuated the up‐regulated mRNA expression of leptin and CCL2. Furthermore, down‐regulated intestinal ZO‐1 protein expression was also inhibited by 5‐ALA/SFC. Moreover, the expressions of HO‐1, HO‐2, Sirt1, and related signal transduction molecules in liver were increased by 5‐ALA/SFC. These results demonstrated that 5‐ALA/SFC treatment ameliorated binge alcohol exposure liver injury in a rat model of HIV‐infected patients by reducing macrophage activation and expression of inflammatory cytokines/chemokines, and by inducing HO‐1, HO‐2, and Sirt1 expression.
Conclusions
Taken together, these findings suggested that treatment with 5‐ALA/SFC has a potential therapeutic effect for binge alcohol exposure liver injury in HIV‐infected patients.
Protective effect of 5‐ALA and SFC against binge alcohol‐induced gut leakiness and inflammatory liver disease in HIV transgenic rats which are model of HIV‐infected patients was investigated. 5‐ALA/SFC treatment improved biochemical and histochemical profiles, attenuated the up‐regulated mRNA expression of leptin and the downstream target chemokine CCL2, increased the expressions of HO‐1, HO‐2, Sirt1 in liver, and inhibited down‐regulated intestinal ZO‐1 expression. These results suggested a potential therapeutic effect for binge alcohol exposure liver injury in HIV patients. |
| doi_str_mv | 10.1111/acer.14117 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2267720512</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2267720512</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4237-d8913d29d944bb00d42e3d59e5a2baeb71dae24882bf513d2398ccc836b031673</originalsourceid><addsrcrecordid>eNp9kMtKw0AUQAdRbK1u_AAZcCeknUeey1j7goCg1W2YzNzW1HRSZxKlu36C4B_2S0xtdendXLgczoWD0CUlXdpMT0gwXepSGhyhNvU4cQgLgmPUJtT1HJ-QsIXOrF0QQtzQ909Ri9MdHpI2Mt528xknce9x2MdxVYGuRQUK3-Z6DjguZPlSFtvN10SrWjb3UV3hBMRrrsFaLLTCEz0rxHIpqtKscZK_g8F3uQVhAecajyfPeGqEtnPQucQPorLn6GQmCgsXh91BT8PBtD92kvvRpB8njnQZDxwVRpQrFqnIdbOMEOUy4MqLwBMsE5AFVAlgbhiybObtSB6FUsqQ-xnh1A94B13vvStTvtVgq3RR1kY3L1PG_CBgxKOsoW72lDSltQZm6crkS2HWKSXpLm-6y5v-5G3gq4Oyzpag_tDfng1A98BHXsD6H1Ua9wcPe-k3KpKFJw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2267720512</pqid></control><display><type>article</type><title>5‐ALA/SFC Attenuated Binge Alcohol–Induced Gut Leakiness and Inflammatory Liver Disease in HIV Transgenic Rats</title><source>Wiley</source><creator>Liu, Chi ; Zhu, Ping ; Fujino, Masayuki ; Zhu, Shuoji ; Ito, Hidenori ; Takahashi, Kiwamu ; Nakajima, Motowo ; Tanaka, Tohru ; Zhuang, Jian ; Li, Xiao‐Kang</creator><creatorcontrib>Liu, Chi ; Zhu, Ping ; Fujino, Masayuki ; Zhu, Shuoji ; Ito, Hidenori ; Takahashi, Kiwamu ; Nakajima, Motowo ; Tanaka, Tohru ; Zhuang, Jian ; Li, Xiao‐Kang</creatorcontrib><description>Background
This study aimed to investigate the protective effect of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) against binge alcohol–induced gut leakiness and inflammatory liver disease in HIV transgenic (TG) rats.
Methods
TG rats were treated with 3 consecutive doses of binge ethanol (EtOH) with or without 5‐ALA/SFC. Blood and liver tissue samples were collected at 6 hours following the last dose of EtOH.
Results
Compared with the wild‐type (WT) rats, the TG rats showed increased sensitivity to alcohol‐mediated inflammation, as evidenced by the significantly elevated levels of serum endotoxin, AST, ALT, ED1, and ED2 staining in liver. In contrast, 5‐ALA/SFC improved the above biochemical and histochemical profiles. 5‐ALA/SFC also attenuated the up‐regulated mRNA expression of leptin and CCL2. Furthermore, down‐regulated intestinal ZO‐1 protein expression was also inhibited by 5‐ALA/SFC. Moreover, the expressions of HO‐1, HO‐2, Sirt1, and related signal transduction molecules in liver were increased by 5‐ALA/SFC. These results demonstrated that 5‐ALA/SFC treatment ameliorated binge alcohol exposure liver injury in a rat model of HIV‐infected patients by reducing macrophage activation and expression of inflammatory cytokines/chemokines, and by inducing HO‐1, HO‐2, and Sirt1 expression.
Conclusions
Taken together, these findings suggested that treatment with 5‐ALA/SFC has a potential therapeutic effect for binge alcohol exposure liver injury in HIV‐infected patients.
Protective effect of 5‐ALA and SFC against binge alcohol‐induced gut leakiness and inflammatory liver disease in HIV transgenic rats which are model of HIV‐infected patients was investigated. 5‐ALA/SFC treatment improved biochemical and histochemical profiles, attenuated the up‐regulated mRNA expression of leptin and the downstream target chemokine CCL2, increased the expressions of HO‐1, HO‐2, Sirt1 in liver, and inhibited down‐regulated intestinal ZO‐1 expression. These results suggested a potential therapeutic effect for binge alcohol exposure liver injury in HIV patients.</description><identifier>ISSN: 0145-6008</identifier><identifier>EISSN: 1530-0277</identifier><identifier>DOI: 10.1111/acer.14117</identifier><identifier>PMID: 31141180</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>5‐aminolevulinic acid ; Alanine Transaminase - blood ; Alcohol ; Alcohol use ; Alcoholic beverages ; Aminolevulinic acid ; Aminolevulinic Acid - pharmacology ; Animals ; Antigens, CD - biosynthesis ; Antigens, CD - immunology ; Antigens, Differentiation, Myelomonocytic - biosynthesis ; Antigens, Differentiation, Myelomonocytic - immunology ; Aspartate Aminotransferases - blood ; Binge Alcohol Exposure Liver Injury ; Binge Drinking - physiopathology ; Cell activation ; Cell Adhesion Molecules, Neuronal - genetics ; Chemokines ; Citric acid ; Cytokines ; Digestive system ; Digestive tract ; Endotoxins ; Endotoxins - blood ; Enterobacteriaceae Infections - microbiology ; Ethanol ; Ethanol - adverse effects ; Exposure ; Ferrous Compounds ; gag Gene Products, Human Immunodeficiency Virus - genetics ; Gene expression ; Heme Oxygenase (Decyclizing) - biosynthesis ; Heme Oxygenase-1 - biosynthesis ; Heme Oxygenase‐1 ; Hepatitis - blood ; Hepatitis - complications ; Hepatitis - prevention & control ; HIV ; HIV Infections - complications ; HIV-1 - genetics ; Human immunodeficiency virus ; Inflammation ; Inflammation Mediators - metabolism ; Intestine ; Intestines - physiopathology ; Leptin ; Lipopolysaccharides - blood ; Liver ; Liver - metabolism ; Liver diseases ; Macrophages ; Monocyte chemoattractant protein 1 ; Permeability - drug effects ; Rats ; Rats, Transgenic ; Receptors, Cell Surface - biosynthesis ; Receptors, Cell Surface - immunology ; Signal transduction ; SIRT1 protein ; Sirtuin 1 - biosynthesis ; Sodium ; Stem Cells ; Triglycerides - metabolism ; Zonula Occludens-1 Protein - biosynthesis</subject><ispartof>Alcoholism, clinical and experimental research, 2019-08, Vol.43 (8), p.1651-1661</ispartof><rights>2019 by the Research Society on Alcoholism</rights><rights>2019 by the Research Society on Alcoholism.</rights><rights>2019 Research Society on Alcoholism</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4237-d8913d29d944bb00d42e3d59e5a2baeb71dae24882bf513d2398ccc836b031673</citedby><cites>FETCH-LOGICAL-c4237-d8913d29d944bb00d42e3d59e5a2baeb71dae24882bf513d2398ccc836b031673</cites><orcidid>0000-0002-0897-2141</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Facer.14117$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Facer.14117$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31141180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Chi</creatorcontrib><creatorcontrib>Zhu, Ping</creatorcontrib><creatorcontrib>Fujino, Masayuki</creatorcontrib><creatorcontrib>Zhu, Shuoji</creatorcontrib><creatorcontrib>Ito, Hidenori</creatorcontrib><creatorcontrib>Takahashi, Kiwamu</creatorcontrib><creatorcontrib>Nakajima, Motowo</creatorcontrib><creatorcontrib>Tanaka, Tohru</creatorcontrib><creatorcontrib>Zhuang, Jian</creatorcontrib><creatorcontrib>Li, Xiao‐Kang</creatorcontrib><title>5‐ALA/SFC Attenuated Binge Alcohol–Induced Gut Leakiness and Inflammatory Liver Disease in HIV Transgenic Rats</title><title>Alcoholism, clinical and experimental research</title><addtitle>Alcohol Clin Exp Res</addtitle><description>Background
This study aimed to investigate the protective effect of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) against binge alcohol–induced gut leakiness and inflammatory liver disease in HIV transgenic (TG) rats.
Methods
TG rats were treated with 3 consecutive doses of binge ethanol (EtOH) with or without 5‐ALA/SFC. Blood and liver tissue samples were collected at 6 hours following the last dose of EtOH.
Results
Compared with the wild‐type (WT) rats, the TG rats showed increased sensitivity to alcohol‐mediated inflammation, as evidenced by the significantly elevated levels of serum endotoxin, AST, ALT, ED1, and ED2 staining in liver. In contrast, 5‐ALA/SFC improved the above biochemical and histochemical profiles. 5‐ALA/SFC also attenuated the up‐regulated mRNA expression of leptin and CCL2. Furthermore, down‐regulated intestinal ZO‐1 protein expression was also inhibited by 5‐ALA/SFC. Moreover, the expressions of HO‐1, HO‐2, Sirt1, and related signal transduction molecules in liver were increased by 5‐ALA/SFC. These results demonstrated that 5‐ALA/SFC treatment ameliorated binge alcohol exposure liver injury in a rat model of HIV‐infected patients by reducing macrophage activation and expression of inflammatory cytokines/chemokines, and by inducing HO‐1, HO‐2, and Sirt1 expression.
Conclusions
Taken together, these findings suggested that treatment with 5‐ALA/SFC has a potential therapeutic effect for binge alcohol exposure liver injury in HIV‐infected patients.
Protective effect of 5‐ALA and SFC against binge alcohol‐induced gut leakiness and inflammatory liver disease in HIV transgenic rats which are model of HIV‐infected patients was investigated. 5‐ALA/SFC treatment improved biochemical and histochemical profiles, attenuated the up‐regulated mRNA expression of leptin and the downstream target chemokine CCL2, increased the expressions of HO‐1, HO‐2, Sirt1 in liver, and inhibited down‐regulated intestinal ZO‐1 expression. These results suggested a potential therapeutic effect for binge alcohol exposure liver injury in HIV patients.</description><subject>5‐aminolevulinic acid</subject><subject>Alanine Transaminase - blood</subject><subject>Alcohol</subject><subject>Alcohol use</subject><subject>Alcoholic beverages</subject><subject>Aminolevulinic acid</subject><subject>Aminolevulinic Acid - pharmacology</subject><subject>Animals</subject><subject>Antigens, CD - biosynthesis</subject><subject>Antigens, CD - immunology</subject><subject>Antigens, Differentiation, Myelomonocytic - biosynthesis</subject><subject>Antigens, Differentiation, Myelomonocytic - immunology</subject><subject>Aspartate Aminotransferases - blood</subject><subject>Binge Alcohol Exposure Liver Injury</subject><subject>Binge Drinking - physiopathology</subject><subject>Cell activation</subject><subject>Cell Adhesion Molecules, Neuronal - genetics</subject><subject>Chemokines</subject><subject>Citric acid</subject><subject>Cytokines</subject><subject>Digestive system</subject><subject>Digestive tract</subject><subject>Endotoxins</subject><subject>Endotoxins - blood</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Ethanol</subject><subject>Ethanol - adverse effects</subject><subject>Exposure</subject><subject>Ferrous Compounds</subject><subject>gag Gene Products, Human Immunodeficiency Virus - genetics</subject><subject>Gene expression</subject><subject>Heme Oxygenase (Decyclizing) - biosynthesis</subject><subject>Heme Oxygenase-1 - biosynthesis</subject><subject>Heme Oxygenase‐1</subject><subject>Hepatitis - blood</subject><subject>Hepatitis - complications</subject><subject>Hepatitis - prevention & control</subject><subject>HIV</subject><subject>HIV Infections - complications</subject><subject>HIV-1 - genetics</subject><subject>Human immunodeficiency virus</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Intestine</subject><subject>Intestines - physiopathology</subject><subject>Leptin</subject><subject>Lipopolysaccharides - blood</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Liver diseases</subject><subject>Macrophages</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Permeability - drug effects</subject><subject>Rats</subject><subject>Rats, Transgenic</subject><subject>Receptors, Cell Surface - biosynthesis</subject><subject>Receptors, Cell Surface - immunology</subject><subject>Signal transduction</subject><subject>SIRT1 protein</subject><subject>Sirtuin 1 - biosynthesis</subject><subject>Sodium</subject><subject>Stem Cells</subject><subject>Triglycerides - metabolism</subject><subject>Zonula Occludens-1 Protein - biosynthesis</subject><issn>0145-6008</issn><issn>1530-0277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKw0AUQAdRbK1u_AAZcCeknUeey1j7goCg1W2YzNzW1HRSZxKlu36C4B_2S0xtdendXLgczoWD0CUlXdpMT0gwXepSGhyhNvU4cQgLgmPUJtT1HJ-QsIXOrF0QQtzQ909Ri9MdHpI2Mt528xknce9x2MdxVYGuRQUK3-Z6DjguZPlSFtvN10SrWjb3UV3hBMRrrsFaLLTCEz0rxHIpqtKscZK_g8F3uQVhAecajyfPeGqEtnPQucQPorLn6GQmCgsXh91BT8PBtD92kvvRpB8njnQZDxwVRpQrFqnIdbOMEOUy4MqLwBMsE5AFVAlgbhiybObtSB6FUsqQ-xnh1A94B13vvStTvtVgq3RR1kY3L1PG_CBgxKOsoW72lDSltQZm6crkS2HWKSXpLm-6y5v-5G3gq4Oyzpag_tDfng1A98BHXsD6H1Ua9wcPe-k3KpKFJw</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Liu, Chi</creator><creator>Zhu, Ping</creator><creator>Fujino, Masayuki</creator><creator>Zhu, Shuoji</creator><creator>Ito, Hidenori</creator><creator>Takahashi, Kiwamu</creator><creator>Nakajima, Motowo</creator><creator>Tanaka, Tohru</creator><creator>Zhuang, Jian</creator><creator>Li, Xiao‐Kang</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>K7.</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-0897-2141</orcidid></search><sort><creationdate>201908</creationdate><title>5‐ALA/SFC Attenuated Binge Alcohol–Induced Gut Leakiness and Inflammatory Liver Disease in HIV Transgenic Rats</title><author>Liu, Chi ; Zhu, Ping ; Fujino, Masayuki ; Zhu, Shuoji ; Ito, Hidenori ; Takahashi, Kiwamu ; Nakajima, Motowo ; Tanaka, Tohru ; Zhuang, Jian ; Li, Xiao‐Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4237-d8913d29d944bb00d42e3d59e5a2baeb71dae24882bf513d2398ccc836b031673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>5‐aminolevulinic acid</topic><topic>Alanine Transaminase - blood</topic><topic>Alcohol</topic><topic>Alcohol use</topic><topic>Alcoholic beverages</topic><topic>Aminolevulinic acid</topic><topic>Aminolevulinic Acid - pharmacology</topic><topic>Animals</topic><topic>Antigens, CD - biosynthesis</topic><topic>Antigens, CD - immunology</topic><topic>Antigens, Differentiation, Myelomonocytic - biosynthesis</topic><topic>Antigens, Differentiation, Myelomonocytic - immunology</topic><topic>Aspartate Aminotransferases - blood</topic><topic>Binge Alcohol Exposure Liver Injury</topic><topic>Binge Drinking - physiopathology</topic><topic>Cell activation</topic><topic>Cell Adhesion Molecules, Neuronal - genetics</topic><topic>Chemokines</topic><topic>Citric acid</topic><topic>Cytokines</topic><topic>Digestive system</topic><topic>Digestive tract</topic><topic>Endotoxins</topic><topic>Endotoxins - blood</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Ethanol</topic><topic>Ethanol - adverse effects</topic><topic>Exposure</topic><topic>Ferrous Compounds</topic><topic>gag Gene Products, Human Immunodeficiency Virus - genetics</topic><topic>Gene expression</topic><topic>Heme Oxygenase (Decyclizing) - biosynthesis</topic><topic>Heme Oxygenase-1 - biosynthesis</topic><topic>Heme Oxygenase‐1</topic><topic>Hepatitis - blood</topic><topic>Hepatitis - complications</topic><topic>Hepatitis - prevention & control</topic><topic>HIV</topic><topic>HIV Infections - complications</topic><topic>HIV-1 - genetics</topic><topic>Human immunodeficiency virus</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Intestine</topic><topic>Intestines - physiopathology</topic><topic>Leptin</topic><topic>Lipopolysaccharides - blood</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Liver diseases</topic><topic>Macrophages</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Permeability - drug effects</topic><topic>Rats</topic><topic>Rats, Transgenic</topic><topic>Receptors, Cell Surface - biosynthesis</topic><topic>Receptors, Cell Surface - immunology</topic><topic>Signal transduction</topic><topic>SIRT1 protein</topic><topic>Sirtuin 1 - biosynthesis</topic><topic>Sodium</topic><topic>Stem Cells</topic><topic>Triglycerides - metabolism</topic><topic>Zonula Occludens-1 Protein - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Chi</creatorcontrib><creatorcontrib>Zhu, Ping</creatorcontrib><creatorcontrib>Fujino, Masayuki</creatorcontrib><creatorcontrib>Zhu, Shuoji</creatorcontrib><creatorcontrib>Ito, Hidenori</creatorcontrib><creatorcontrib>Takahashi, Kiwamu</creatorcontrib><creatorcontrib>Nakajima, Motowo</creatorcontrib><creatorcontrib>Tanaka, Tohru</creatorcontrib><creatorcontrib>Zhuang, Jian</creatorcontrib><creatorcontrib>Li, Xiao‐Kang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Alcoholism, clinical and experimental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Chi</au><au>Zhu, Ping</au><au>Fujino, Masayuki</au><au>Zhu, Shuoji</au><au>Ito, Hidenori</au><au>Takahashi, Kiwamu</au><au>Nakajima, Motowo</au><au>Tanaka, Tohru</au><au>Zhuang, Jian</au><au>Li, Xiao‐Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>5‐ALA/SFC Attenuated Binge Alcohol–Induced Gut Leakiness and Inflammatory Liver Disease in HIV Transgenic Rats</atitle><jtitle>Alcoholism, clinical and experimental research</jtitle><addtitle>Alcohol Clin Exp Res</addtitle><date>2019-08</date><risdate>2019</risdate><volume>43</volume><issue>8</issue><spage>1651</spage><epage>1661</epage><pages>1651-1661</pages><issn>0145-6008</issn><eissn>1530-0277</eissn><abstract>Background
This study aimed to investigate the protective effect of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) against binge alcohol–induced gut leakiness and inflammatory liver disease in HIV transgenic (TG) rats.
Methods
TG rats were treated with 3 consecutive doses of binge ethanol (EtOH) with or without 5‐ALA/SFC. Blood and liver tissue samples were collected at 6 hours following the last dose of EtOH.
Results
Compared with the wild‐type (WT) rats, the TG rats showed increased sensitivity to alcohol‐mediated inflammation, as evidenced by the significantly elevated levels of serum endotoxin, AST, ALT, ED1, and ED2 staining in liver. In contrast, 5‐ALA/SFC improved the above biochemical and histochemical profiles. 5‐ALA/SFC also attenuated the up‐regulated mRNA expression of leptin and CCL2. Furthermore, down‐regulated intestinal ZO‐1 protein expression was also inhibited by 5‐ALA/SFC. Moreover, the expressions of HO‐1, HO‐2, Sirt1, and related signal transduction molecules in liver were increased by 5‐ALA/SFC. These results demonstrated that 5‐ALA/SFC treatment ameliorated binge alcohol exposure liver injury in a rat model of HIV‐infected patients by reducing macrophage activation and expression of inflammatory cytokines/chemokines, and by inducing HO‐1, HO‐2, and Sirt1 expression.
Conclusions
Taken together, these findings suggested that treatment with 5‐ALA/SFC has a potential therapeutic effect for binge alcohol exposure liver injury in HIV‐infected patients.
Protective effect of 5‐ALA and SFC against binge alcohol‐induced gut leakiness and inflammatory liver disease in HIV transgenic rats which are model of HIV‐infected patients was investigated. 5‐ALA/SFC treatment improved biochemical and histochemical profiles, attenuated the up‐regulated mRNA expression of leptin and the downstream target chemokine CCL2, increased the expressions of HO‐1, HO‐2, Sirt1 in liver, and inhibited down‐regulated intestinal ZO‐1 expression. These results suggested a potential therapeutic effect for binge alcohol exposure liver injury in HIV patients.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31141180</pmid><doi>10.1111/acer.14117</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-0897-2141</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-6008 |
| ispartof | Alcoholism, clinical and experimental research, 2019-08, Vol.43 (8), p.1651-1661 |
| issn | 0145-6008 1530-0277 |
| language | eng |
| recordid | cdi_proquest_journals_2267720512 |
| source | Wiley |
| subjects | 5‐aminolevulinic acid Alanine Transaminase - blood Alcohol Alcohol use Alcoholic beverages Aminolevulinic acid Aminolevulinic Acid - pharmacology Animals Antigens, CD - biosynthesis Antigens, CD - immunology Antigens, Differentiation, Myelomonocytic - biosynthesis Antigens, Differentiation, Myelomonocytic - immunology Aspartate Aminotransferases - blood Binge Alcohol Exposure Liver Injury Binge Drinking - physiopathology Cell activation Cell Adhesion Molecules, Neuronal - genetics Chemokines Citric acid Cytokines Digestive system Digestive tract Endotoxins Endotoxins - blood Enterobacteriaceae Infections - microbiology Ethanol Ethanol - adverse effects Exposure Ferrous Compounds gag Gene Products, Human Immunodeficiency Virus - genetics Gene expression Heme Oxygenase (Decyclizing) - biosynthesis Heme Oxygenase-1 - biosynthesis Heme Oxygenase‐1 Hepatitis - blood Hepatitis - complications Hepatitis - prevention & control HIV HIV Infections - complications HIV-1 - genetics Human immunodeficiency virus Inflammation Inflammation Mediators - metabolism Intestine Intestines - physiopathology Leptin Lipopolysaccharides - blood Liver Liver - metabolism Liver diseases Macrophages Monocyte chemoattractant protein 1 Permeability - drug effects Rats Rats, Transgenic Receptors, Cell Surface - biosynthesis Receptors, Cell Surface - immunology Signal transduction SIRT1 protein Sirtuin 1 - biosynthesis Sodium Stem Cells Triglycerides - metabolism Zonula Occludens-1 Protein - biosynthesis |
| title | 5‐ALA/SFC Attenuated Binge Alcohol–Induced Gut Leakiness and Inflammatory Liver Disease in HIV Transgenic Rats |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T20%3A13%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=5%E2%80%90ALA/SFC%20Attenuated%20Binge%20Alcohol%E2%80%93Induced%20Gut%20Leakiness%20and%20Inflammatory%20Liver%20Disease%20in%20HIV%20Transgenic%20Rats&rft.jtitle=Alcoholism,%20clinical%20and%20experimental%20research&rft.au=Liu,%20Chi&rft.date=2019-08&rft.volume=43&rft.issue=8&rft.spage=1651&rft.epage=1661&rft.pages=1651-1661&rft.issn=0145-6008&rft.eissn=1530-0277&rft_id=info:doi/10.1111/acer.14117&rft_dat=%3Cproquest_cross%3E2267720512%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4237-d8913d29d944bb00d42e3d59e5a2baeb71dae24882bf513d2398ccc836b031673%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2267720512&rft_id=info:pmid/31141180&rfr_iscdi=true |