Loading…
New developments in online OUR monitoring and its application to animal cell cultures
The increasing demand for biopharmaceuticals produced in mammalian cells has driven the industry to enhance the productivity of bioprocesses through intensification of culture process. Fed-batch and perfusion culturing strategies are considered the most attractive choices, but the application of the...
Saved in:
| Published in: | Applied microbiology and biotechnology 2019-09, Vol.103 (17), p.6903-6917 |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33 |
| container_end_page | 6917 |
| container_issue | 17 |
| container_start_page | 6903 |
| container_title | Applied microbiology and biotechnology |
| container_volume | 103 |
| creator | Martínez-Monge, I. Roman, R. Comas, P. Fontova, A. Lecina, M. Casablancas, A. Cairó, J. J. |
| description | The increasing demand for biopharmaceuticals produced in mammalian cells has driven the industry to enhance the productivity of bioprocesses through intensification of culture process. Fed-batch and perfusion culturing strategies are considered the most attractive choices, but the application of these processes requires the availability of reliable online measuring systems for the estimation of cell density and metabolic activity. This manuscript reviews the methods (and the devices used) for monitoring of the oxygen consumption, also known as oxygen uptake rate (OUR), since it is a straightforward parameter to estimate viable cell density and the physiological state of cells. Furthermore, as oxygen plays an important role in the cell metabolism, OUR has also been very useful to estimate nutrient consumption, especially the carbon (glucose and glutamine) and nitrogen (glutamine) sources. Three different methods for the measurement of OUR have been developed up to date, being the dynamic method the golden standard, even though DO and pH perturbations generated in the culture during each measurement. For this, many efforts have been focused in developing non-invasive methods, such as global mass balance or stationary liquid mass balance. The low oxygen consumption rates by the cells and the high accuracy required for oxygen concentration measurement in the gas streams (inlet and outlet) have limited the applicability of the global mass balance methodology in mammalian cell cultures. In contrast, stationary liquid mass balance has successfully been implemented showing very similar OUR profiles compared with those obtained with the dynamic method. The huge amount of studies published in the last years evidence that OUR have become a reliable alternative for the monitoring and control of high cell density culturing strategies with very high productivities. |
| doi_str_mv | 10.1007/s00253-019-09989-4 |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2257968080</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A596346813</galeid><sourcerecordid>A596346813</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33</originalsourceid><addsrcrecordid>eNp9kVtrFTEUhYMo9lj9Az7IgE8-TN25TCZ5LMVLoVio7XNIM3sOKTPJmGSq_ntzPNVyQCSQwOZba7OyCHlN4YQC9O8zAOt4C1S3oLXSrXhCNlRw1oKk4inZAO27tu-0OiIvcr4DoExJ-ZwcccpBM6k25OYLfm8GvMcpLjOGkhsfmhgmH7C5vLlq5hh8icmHbWPD0PgK2GWZvLPFx9CUWMd-tlPjcKrXOpU1YX5Jno12yvjq4T0m1x8_XJ99bi8uP52fnV60rqO8tJ2QsleqA9HD7cglCK0Zctej42wYgEnXSQQ20JpOWuiVoDCMQg6casf5MXm7t11S_LZiLuYurinUjYaxrtdSgYJHamsnND6MsSTrZp-dOe205EIquvM6-QdVz4CzdzHg6Ov8QPDuQFCZgj_K1q45m_OvV4cs27MuxZwTjmZJ9dfST0PB7Lo0-y5N7dL87tKIKnrzkG69nXH4K_lTXgX4HsjLriBMj_H_Y_sLYFal3A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2257968080</pqid></control><display><type>article</type><title>New developments in online OUR monitoring and its application to animal cell cultures</title><source>ABI/INFORM Collection</source><source>Springer Link</source><creator>Martínez-Monge, I. ; Roman, R. ; Comas, P. ; Fontova, A. ; Lecina, M. ; Casablancas, A. ; Cairó, J. J.</creator><creatorcontrib>Martínez-Monge, I. ; Roman, R. ; Comas, P. ; Fontova, A. ; Lecina, M. ; Casablancas, A. ; Cairó, J. J.</creatorcontrib><description>The increasing demand for biopharmaceuticals produced in mammalian cells has driven the industry to enhance the productivity of bioprocesses through intensification of culture process. Fed-batch and perfusion culturing strategies are considered the most attractive choices, but the application of these processes requires the availability of reliable online measuring systems for the estimation of cell density and metabolic activity. This manuscript reviews the methods (and the devices used) for monitoring of the oxygen consumption, also known as oxygen uptake rate (OUR), since it is a straightforward parameter to estimate viable cell density and the physiological state of cells. Furthermore, as oxygen plays an important role in the cell metabolism, OUR has also been very useful to estimate nutrient consumption, especially the carbon (glucose and glutamine) and nitrogen (glutamine) sources. Three different methods for the measurement of OUR have been developed up to date, being the dynamic method the golden standard, even though DO and pH perturbations generated in the culture during each measurement. For this, many efforts have been focused in developing non-invasive methods, such as global mass balance or stationary liquid mass balance. The low oxygen consumption rates by the cells and the high accuracy required for oxygen concentration measurement in the gas streams (inlet and outlet) have limited the applicability of the global mass balance methodology in mammalian cell cultures. In contrast, stationary liquid mass balance has successfully been implemented showing very similar OUR profiles compared with those obtained with the dynamic method. The huge amount of studies published in the last years evidence that OUR have become a reliable alternative for the monitoring and control of high cell density culturing strategies with very high productivities.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-019-09989-4</identifier><identifier>PMID: 31309268</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Batch Cell Culture Techniques - instrumentation ; Batch Cell Culture Techniques - methods ; Batch culture ; Biological products ; Biomedical and Life Sciences ; Bioreactors ; Biotechnology ; Cell Count ; Cell culture ; Cell density ; Cells ; Culture Media - chemistry ; Density ; Gas streams ; Glutamine ; Innovations ; Internet ; Life Sciences ; Mammalian cells ; Mammals ; Mass balance ; Measurement methods ; Metabolism ; Microbial Genetics and Genomics ; Microbiology ; Mini-Review ; Monitoring ; Nutrient sources ; Nutrients - analysis ; Nutrients - metabolism ; On-line systems ; Online Systems ; Oxygen ; Oxygen - analysis ; Oxygen - metabolism ; Oxygen Consumption ; Oxygen uptake ; Parameter estimation ; Perfusion</subject><ispartof>Applied microbiology and biotechnology, 2019-09, Vol.103 (17), p.6903-6917</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33</citedby><cites>FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33</cites><orcidid>0000-0002-1739-8815</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2257968080/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2257968080?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,786,790,11715,27957,27958,36095,44398,75252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31309268$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martínez-Monge, I.</creatorcontrib><creatorcontrib>Roman, R.</creatorcontrib><creatorcontrib>Comas, P.</creatorcontrib><creatorcontrib>Fontova, A.</creatorcontrib><creatorcontrib>Lecina, M.</creatorcontrib><creatorcontrib>Casablancas, A.</creatorcontrib><creatorcontrib>Cairó, J. J.</creatorcontrib><title>New developments in online OUR monitoring and its application to animal cell cultures</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The increasing demand for biopharmaceuticals produced in mammalian cells has driven the industry to enhance the productivity of bioprocesses through intensification of culture process. Fed-batch and perfusion culturing strategies are considered the most attractive choices, but the application of these processes requires the availability of reliable online measuring systems for the estimation of cell density and metabolic activity. This manuscript reviews the methods (and the devices used) for monitoring of the oxygen consumption, also known as oxygen uptake rate (OUR), since it is a straightforward parameter to estimate viable cell density and the physiological state of cells. Furthermore, as oxygen plays an important role in the cell metabolism, OUR has also been very useful to estimate nutrient consumption, especially the carbon (glucose and glutamine) and nitrogen (glutamine) sources. Three different methods for the measurement of OUR have been developed up to date, being the dynamic method the golden standard, even though DO and pH perturbations generated in the culture during each measurement. For this, many efforts have been focused in developing non-invasive methods, such as global mass balance or stationary liquid mass balance. The low oxygen consumption rates by the cells and the high accuracy required for oxygen concentration measurement in the gas streams (inlet and outlet) have limited the applicability of the global mass balance methodology in mammalian cell cultures. In contrast, stationary liquid mass balance has successfully been implemented showing very similar OUR profiles compared with those obtained with the dynamic method. The huge amount of studies published in the last years evidence that OUR have become a reliable alternative for the monitoring and control of high cell density culturing strategies with very high productivities.</description><subject>Animals</subject><subject>Batch Cell Culture Techniques - instrumentation</subject><subject>Batch Cell Culture Techniques - methods</subject><subject>Batch culture</subject><subject>Biological products</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell Count</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cells</subject><subject>Culture Media - chemistry</subject><subject>Density</subject><subject>Gas streams</subject><subject>Glutamine</subject><subject>Innovations</subject><subject>Internet</subject><subject>Life Sciences</subject><subject>Mammalian cells</subject><subject>Mammals</subject><subject>Mass balance</subject><subject>Measurement methods</subject><subject>Metabolism</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mini-Review</subject><subject>Monitoring</subject><subject>Nutrient sources</subject><subject>Nutrients - analysis</subject><subject>Nutrients - metabolism</subject><subject>On-line systems</subject><subject>Online Systems</subject><subject>Oxygen</subject><subject>Oxygen - analysis</subject><subject>Oxygen - metabolism</subject><subject>Oxygen Consumption</subject><subject>Oxygen uptake</subject><subject>Parameter estimation</subject><subject>Perfusion</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNp9kVtrFTEUhYMo9lj9Az7IgE8-TN25TCZ5LMVLoVio7XNIM3sOKTPJmGSq_ntzPNVyQCSQwOZba7OyCHlN4YQC9O8zAOt4C1S3oLXSrXhCNlRw1oKk4inZAO27tu-0OiIvcr4DoExJ-ZwcccpBM6k25OYLfm8GvMcpLjOGkhsfmhgmH7C5vLlq5hh8icmHbWPD0PgK2GWZvLPFx9CUWMd-tlPjcKrXOpU1YX5Jno12yvjq4T0m1x8_XJ99bi8uP52fnV60rqO8tJ2QsleqA9HD7cglCK0Zctej42wYgEnXSQQ20JpOWuiVoDCMQg6casf5MXm7t11S_LZiLuYurinUjYaxrtdSgYJHamsnND6MsSTrZp-dOe205EIquvM6-QdVz4CzdzHg6Ov8QPDuQFCZgj_K1q45m_OvV4cs27MuxZwTjmZJ9dfST0PB7Lo0-y5N7dL87tKIKnrzkG69nXH4K_lTXgX4HsjLriBMj_H_Y_sLYFal3A</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Martínez-Monge, I.</creator><creator>Roman, R.</creator><creator>Comas, P.</creator><creator>Fontova, A.</creator><creator>Lecina, M.</creator><creator>Casablancas, A.</creator><creator>Cairó, J. J.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><orcidid>https://orcid.org/0000-0002-1739-8815</orcidid></search><sort><creationdate>20190901</creationdate><title>New developments in online OUR monitoring and its application to animal cell cultures</title><author>Martínez-Monge, I. ; Roman, R. ; Comas, P. ; Fontova, A. ; Lecina, M. ; Casablancas, A. ; Cairó, J. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Batch Cell Culture Techniques - instrumentation</topic><topic>Batch Cell Culture Techniques - methods</topic><topic>Batch culture</topic><topic>Biological products</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Cell Count</topic><topic>Cell culture</topic><topic>Cell density</topic><topic>Cells</topic><topic>Culture Media - chemistry</topic><topic>Density</topic><topic>Gas streams</topic><topic>Glutamine</topic><topic>Innovations</topic><topic>Internet</topic><topic>Life Sciences</topic><topic>Mammalian cells</topic><topic>Mammals</topic><topic>Mass balance</topic><topic>Measurement methods</topic><topic>Metabolism</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mini-Review</topic><topic>Monitoring</topic><topic>Nutrient sources</topic><topic>Nutrients - analysis</topic><topic>Nutrients - metabolism</topic><topic>On-line systems</topic><topic>Online Systems</topic><topic>Oxygen</topic><topic>Oxygen - analysis</topic><topic>Oxygen - metabolism</topic><topic>Oxygen Consumption</topic><topic>Oxygen uptake</topic><topic>Parameter estimation</topic><topic>Perfusion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martínez-Monge, I.</creatorcontrib><creatorcontrib>Roman, R.</creatorcontrib><creatorcontrib>Comas, P.</creatorcontrib><creatorcontrib>Fontova, A.</creatorcontrib><creatorcontrib>Lecina, M.</creatorcontrib><creatorcontrib>Casablancas, A.</creatorcontrib><creatorcontrib>Cairó, J. J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI-INFORM Complete</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Business Premium Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Biological Sciences</collection><collection>ABI/INFORM Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez-Monge, I.</au><au>Roman, R.</au><au>Comas, P.</au><au>Fontova, A.</au><au>Lecina, M.</au><au>Casablancas, A.</au><au>Cairó, J. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New developments in online OUR monitoring and its application to animal cell cultures</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>103</volume><issue>17</issue><spage>6903</spage><epage>6917</epage><pages>6903-6917</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>The increasing demand for biopharmaceuticals produced in mammalian cells has driven the industry to enhance the productivity of bioprocesses through intensification of culture process. Fed-batch and perfusion culturing strategies are considered the most attractive choices, but the application of these processes requires the availability of reliable online measuring systems for the estimation of cell density and metabolic activity. This manuscript reviews the methods (and the devices used) for monitoring of the oxygen consumption, also known as oxygen uptake rate (OUR), since it is a straightforward parameter to estimate viable cell density and the physiological state of cells. Furthermore, as oxygen plays an important role in the cell metabolism, OUR has also been very useful to estimate nutrient consumption, especially the carbon (glucose and glutamine) and nitrogen (glutamine) sources. Three different methods for the measurement of OUR have been developed up to date, being the dynamic method the golden standard, even though DO and pH perturbations generated in the culture during each measurement. For this, many efforts have been focused in developing non-invasive methods, such as global mass balance or stationary liquid mass balance. The low oxygen consumption rates by the cells and the high accuracy required for oxygen concentration measurement in the gas streams (inlet and outlet) have limited the applicability of the global mass balance methodology in mammalian cell cultures. In contrast, stationary liquid mass balance has successfully been implemented showing very similar OUR profiles compared with those obtained with the dynamic method. The huge amount of studies published in the last years evidence that OUR have become a reliable alternative for the monitoring and control of high cell density culturing strategies with very high productivities.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31309268</pmid><doi>10.1007/s00253-019-09989-4</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-1739-8815</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2019-09, Vol.103 (17), p.6903-6917 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_journals_2257968080 |
| source | ABI/INFORM Collection; Springer Link |
| subjects | Animals Batch Cell Culture Techniques - instrumentation Batch Cell Culture Techniques - methods Batch culture Biological products Biomedical and Life Sciences Bioreactors Biotechnology Cell Count Cell culture Cell density Cells Culture Media - chemistry Density Gas streams Glutamine Innovations Internet Life Sciences Mammalian cells Mammals Mass balance Measurement methods Metabolism Microbial Genetics and Genomics Microbiology Mini-Review Monitoring Nutrient sources Nutrients - analysis Nutrients - metabolism On-line systems Online Systems Oxygen Oxygen - analysis Oxygen - metabolism Oxygen Consumption Oxygen uptake Parameter estimation Perfusion |
| title | New developments in online OUR monitoring and its application to animal cell cultures |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T07%3A38%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=New%20developments%20in%20online%20OUR%20monitoring%20and%20its%20application%20to%20animal%20cell%20cultures&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Mart%C3%ADnez-Monge,%20I.&rft.date=2019-09-01&rft.volume=103&rft.issue=17&rft.spage=6903&rft.epage=6917&rft.pages=6903-6917&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-019-09989-4&rft_dat=%3Cgale_proqu%3EA596346813%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c513t-546678850470bf3604992e3c7ec32dd026c56e02d19896a078410df46d319c33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2257968080&rft_id=info:pmid/31309268&rft_galeid=A596346813&rfr_iscdi=true |