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A simple and effective method to obtain high DNA quality and quantity from Cerrado plant species
Despite the importance in conservation and breeding purposes, molecular studies using Cerrado plant species are still rare, mainly because of their high amounts of secondary compounds, impeding DNA extraction to downstream applications, such as PCR amplification. To date, the DNA extraction methods...
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| Published in: | Molecular biology reports 2019-08, Vol.46 (4), p.4611-4615 |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c375t-c6683100993fc63ef5dea0c3bc6f77d1760af890c71177efcab1b6d8570fa7a03 |
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| container_title | Molecular biology reports |
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| creator | Souza, Diego Cerveira de Teixeira, Terezinha Aparecida |
| description | Despite the importance in conservation and breeding purposes, molecular studies using Cerrado plant species are still rare, mainly because of their high amounts of secondary compounds, impeding DNA extraction to downstream applications, such as PCR amplification. To date, the DNA extraction methods described are sometimes inadequate for these species, expensive, time-intensive and/or use very toxic reagents. Here, we present a simple and effective method, based on SDS and Triton X-100, to obtain high DNA quality and quantity from Cerrado plant species for molecular biological techniques. The DNA obtained by our protocol was free of contaminants and excellent for enzymatic restriction and PCR amplification. The concentration of extracted DNA across all tested species ranged from 156 to 1166 ng µL
−1
(1.56–11.66 µg g
−1
of dry tissue), with an A
260
/A
280
ratio from 1.78 to 1.92. The new DNA extraction protocol described here provides high DNA quality and quantity from Cerrado plant species in a fast, simple and less toxic way. Thus, the use of our method will allow ecologists, geneticists and breeders to rapidly obtain high-quality and -quantity DNA from Cerrado plant species for any molecular biology study. |
| doi_str_mv | 10.1007/s11033-019-04845-0 |
| format | article |
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−1
(1.56–11.66 µg g
−1
of dry tissue), with an A
260
/A
280
ratio from 1.78 to 1.92. The new DNA extraction protocol described here provides high DNA quality and quantity from Cerrado plant species in a fast, simple and less toxic way. Thus, the use of our method will allow ecologists, geneticists and breeders to rapidly obtain high-quality and -quantity DNA from Cerrado plant species for any molecular biology study.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-019-04845-0</identifier><identifier>PMID: 31073915</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Brazil ; Contaminants ; Deoxyribonucleic acid ; DNA ; DNA - isolation & purification ; DNA, Plant - isolation & purification ; Flowers & plants ; Histology ; Life Sciences ; Morphology ; Plants - genetics ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Rare species ; Short Communication</subject><ispartof>Molecular biology reports, 2019-08, Vol.46 (4), p.4611-4615</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Molecular Biology Reports is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-c6683100993fc63ef5dea0c3bc6f77d1760af890c71177efcab1b6d8570fa7a03</citedby><cites>FETCH-LOGICAL-c375t-c6683100993fc63ef5dea0c3bc6f77d1760af890c71177efcab1b6d8570fa7a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31073915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Souza, Diego Cerveira de</creatorcontrib><creatorcontrib>Teixeira, Terezinha Aparecida</creatorcontrib><title>A simple and effective method to obtain high DNA quality and quantity from Cerrado plant species</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Despite the importance in conservation and breeding purposes, molecular studies using Cerrado plant species are still rare, mainly because of their high amounts of secondary compounds, impeding DNA extraction to downstream applications, such as PCR amplification. To date, the DNA extraction methods described are sometimes inadequate for these species, expensive, time-intensive and/or use very toxic reagents. Here, we present a simple and effective method, based on SDS and Triton X-100, to obtain high DNA quality and quantity from Cerrado plant species for molecular biological techniques. The DNA obtained by our protocol was free of contaminants and excellent for enzymatic restriction and PCR amplification. The concentration of extracted DNA across all tested species ranged from 156 to 1166 ng µL
−1
(1.56–11.66 µg g
−1
of dry tissue), with an A
260
/A
280
ratio from 1.78 to 1.92. The new DNA extraction protocol described here provides high DNA quality and quantity from Cerrado plant species in a fast, simple and less toxic way. 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To date, the DNA extraction methods described are sometimes inadequate for these species, expensive, time-intensive and/or use very toxic reagents. Here, we present a simple and effective method, based on SDS and Triton X-100, to obtain high DNA quality and quantity from Cerrado plant species for molecular biological techniques. The DNA obtained by our protocol was free of contaminants and excellent for enzymatic restriction and PCR amplification. The concentration of extracted DNA across all tested species ranged from 156 to 1166 ng µL
−1
(1.56–11.66 µg g
−1
of dry tissue), with an A
260
/A
280
ratio from 1.78 to 1.92. The new DNA extraction protocol described here provides high DNA quality and quantity from Cerrado plant species in a fast, simple and less toxic way. Thus, the use of our method will allow ecologists, geneticists and breeders to rapidly obtain high-quality and -quantity DNA from Cerrado plant species for any molecular biology study.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31073915</pmid><doi>10.1007/s11033-019-04845-0</doi><tpages>5</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Brazil Contaminants Deoxyribonucleic acid DNA DNA - isolation & purification DNA, Plant - isolation & purification Flowers & plants Histology Life Sciences Morphology Plants - genetics Polymerase chain reaction Polymerase Chain Reaction - methods Rare species Short Communication |
| title | A simple and effective method to obtain high DNA quality and quantity from Cerrado plant species |
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