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Production of a novel killer toxin from Saccharomyces eubayanus using agro-industrial waste and its application against wine spoilage yeasts
The juicing industry generates large amounts of waste that mostly lack commercial value and, in the absence of waste treatment policies, produces environmental pollution. Also, microbiological spoilage is a major concern in the wine industry and control tools are limited. Taking these challenges int...
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| Published in: | Antonie van Leeuwenhoek 2019-07, Vol.112 (7), p.965-973 |
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| container_title | Antonie van Leeuwenhoek |
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| creator | Mazzucco, María Belén Ganga, María Angélica Sangorrín, Marcela Paula |
| description | The juicing industry generates large amounts of waste that mostly lack commercial value and, in the absence of waste treatment policies, produces environmental pollution. Also, microbiological spoilage is a major concern in the wine industry and control tools are limited. Taking these challenges into account, agro-industrial waste coming from ultrafiltrated apple and pear juice were used to grow
Saccharomyces eubayanus
and to produce its killer toxin (SeKT). A Plackett–Burman screening was performed in order to optimize SeKT production in ultrafiltrated apple and pear juice. The optimized medium was characterized: 75% v/v WUJ, 0.5% m/v KH
2
PO
4
, 0.5% m/v MgSO
4
, 0.5% m/v (NH
4
)SO
4
, 0.5% g/L urea, 10% v/v glycerol and 0.1% v/v Triton X-100. SeKT produced in WUJ optimised medium was used to perform killer assays against wine spoilage yeasts and showed antagonistic activity against
Brettanomyces bruxellensis
,
Pichia guilliermondii
,
Pichia manshurica
and
Pichia membranifaciens
. Different inhibition percentages against spoilage species in a wine environment (49–69%) were detected and preserved for at least 48 h. For the first time, this work reports the ability of
S. eubayanus
to produce a killer toxin with potential use as a biocontrol tool in winemaking. Producing SeKT using agro-industrial waste as an alternative medium to cultivate
S. eubayanus
would have industrial, economic and ecological benefits. |
| doi_str_mv | 10.1007/s10482-019-01231-5 |
| format | article |
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Saccharomyces eubayanus
and to produce its killer toxin (SeKT). A Plackett–Burman screening was performed in order to optimize SeKT production in ultrafiltrated apple and pear juice. The optimized medium was characterized: 75% v/v WUJ, 0.5% m/v KH
2
PO
4
, 0.5% m/v MgSO
4
, 0.5% m/v (NH
4
)SO
4
, 0.5% g/L urea, 10% v/v glycerol and 0.1% v/v Triton X-100. SeKT produced in WUJ optimised medium was used to perform killer assays against wine spoilage yeasts and showed antagonistic activity against
Brettanomyces bruxellensis
,
Pichia guilliermondii
,
Pichia manshurica
and
Pichia membranifaciens
. Different inhibition percentages against spoilage species in a wine environment (49–69%) were detected and preserved for at least 48 h. For the first time, this work reports the ability of
S. eubayanus
to produce a killer toxin with potential use as a biocontrol tool in winemaking. Producing SeKT using agro-industrial waste as an alternative medium to cultivate
S. eubayanus
would have industrial, economic and ecological benefits.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-019-01231-5</identifier><identifier>PMID: 30671692</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agricultural wastes ; Biological control ; Biomedical and Life Sciences ; Culture Media - chemistry ; Culture Media - metabolism ; Environmental policy ; Fermentation ; Fruit juices ; Glycerol ; Industrial Microbiology - instrumentation ; Industrial Microbiology - methods ; Industrial Waste - analysis ; Industrial wastes ; Killer Factors, Yeast - metabolism ; Killer Factors, Yeast - pharmacology ; Life Sciences ; Medical Microbiology ; Microbiology ; Original Paper ; Pichia ; Pichia - drug effects ; Pichia - growth & development ; Plant Sciences ; Potassium phosphate ; Potassium phosphates ; Saccharomyces ; Saccharomyces - chemistry ; Saccharomyces - genetics ; Saccharomyces - metabolism ; Soil Science & Conservation ; Spoilage ; Urea ; Waste Products - analysis ; Waste treatment ; Wine ; Wine - microbiology ; Yeast ; Yeasts</subject><ispartof>Antonie van Leeuwenhoek, 2019-07, Vol.112 (7), p.965-973</ispartof><rights>Springer Nature Switzerland AG 2019</rights><rights>Antonie van Leeuwenhoek is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-8f000c2046b15e7f5ce3cdb7c77cd258449470402b8dd4704364a00c14c1b7ae3</citedby><cites>FETCH-LOGICAL-c375t-8f000c2046b15e7f5ce3cdb7c77cd258449470402b8dd4704364a00c14c1b7ae3</cites><orcidid>0000-0001-5921-7448 ; 0000-0001-7372-8359 ; 0000-0002-4927-9036</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30671692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazzucco, María Belén</creatorcontrib><creatorcontrib>Ganga, María Angélica</creatorcontrib><creatorcontrib>Sangorrín, Marcela Paula</creatorcontrib><title>Production of a novel killer toxin from Saccharomyces eubayanus using agro-industrial waste and its application against wine spoilage yeasts</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>The juicing industry generates large amounts of waste that mostly lack commercial value and, in the absence of waste treatment policies, produces environmental pollution. Also, microbiological spoilage is a major concern in the wine industry and control tools are limited. Taking these challenges into account, agro-industrial waste coming from ultrafiltrated apple and pear juice were used to grow
Saccharomyces eubayanus
and to produce its killer toxin (SeKT). A Plackett–Burman screening was performed in order to optimize SeKT production in ultrafiltrated apple and pear juice. The optimized medium was characterized: 75% v/v WUJ, 0.5% m/v KH
2
PO
4
, 0.5% m/v MgSO
4
, 0.5% m/v (NH
4
)SO
4
, 0.5% g/L urea, 10% v/v glycerol and 0.1% v/v Triton X-100. SeKT produced in WUJ optimised medium was used to perform killer assays against wine spoilage yeasts and showed antagonistic activity against
Brettanomyces bruxellensis
,
Pichia guilliermondii
,
Pichia manshurica
and
Pichia membranifaciens
. Different inhibition percentages against spoilage species in a wine environment (49–69%) were detected and preserved for at least 48 h. For the first time, this work reports the ability of
S. eubayanus
to produce a killer toxin with potential use as a biocontrol tool in winemaking. Producing SeKT using agro-industrial waste as an alternative medium to cultivate
S. eubayanus
would have industrial, economic and ecological benefits.</description><subject>Agricultural wastes</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Culture Media - chemistry</subject><subject>Culture Media - metabolism</subject><subject>Environmental policy</subject><subject>Fermentation</subject><subject>Fruit juices</subject><subject>Glycerol</subject><subject>Industrial Microbiology - instrumentation</subject><subject>Industrial Microbiology - methods</subject><subject>Industrial Waste - analysis</subject><subject>Industrial wastes</subject><subject>Killer Factors, Yeast - metabolism</subject><subject>Killer Factors, Yeast - pharmacology</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Pichia</subject><subject>Pichia - drug effects</subject><subject>Pichia - growth & development</subject><subject>Plant Sciences</subject><subject>Potassium phosphate</subject><subject>Potassium phosphates</subject><subject>Saccharomyces</subject><subject>Saccharomyces - 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Also, microbiological spoilage is a major concern in the wine industry and control tools are limited. Taking these challenges into account, agro-industrial waste coming from ultrafiltrated apple and pear juice were used to grow
Saccharomyces eubayanus
and to produce its killer toxin (SeKT). A Plackett–Burman screening was performed in order to optimize SeKT production in ultrafiltrated apple and pear juice. The optimized medium was characterized: 75% v/v WUJ, 0.5% m/v KH
2
PO
4
, 0.5% m/v MgSO
4
, 0.5% m/v (NH
4
)SO
4
, 0.5% g/L urea, 10% v/v glycerol and 0.1% v/v Triton X-100. SeKT produced in WUJ optimised medium was used to perform killer assays against wine spoilage yeasts and showed antagonistic activity against
Brettanomyces bruxellensis
,
Pichia guilliermondii
,
Pichia manshurica
and
Pichia membranifaciens
. Different inhibition percentages against spoilage species in a wine environment (49–69%) were detected and preserved for at least 48 h. For the first time, this work reports the ability of
S. eubayanus
to produce a killer toxin with potential use as a biocontrol tool in winemaking. Producing SeKT using agro-industrial waste as an alternative medium to cultivate
S. eubayanus
would have industrial, economic and ecological benefits.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>30671692</pmid><doi>10.1007/s10482-019-01231-5</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5921-7448</orcidid><orcidid>https://orcid.org/0000-0001-7372-8359</orcidid><orcidid>https://orcid.org/0000-0002-4927-9036</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-6072 |
| ispartof | Antonie van Leeuwenhoek, 2019-07, Vol.112 (7), p.965-973 |
| issn | 0003-6072 1572-9699 |
| language | eng |
| recordid | cdi_proquest_journals_2169500308 |
| source | Springer Link |
| subjects | Agricultural wastes Biological control Biomedical and Life Sciences Culture Media - chemistry Culture Media - metabolism Environmental policy Fermentation Fruit juices Glycerol Industrial Microbiology - instrumentation Industrial Microbiology - methods Industrial Waste - analysis Industrial wastes Killer Factors, Yeast - metabolism Killer Factors, Yeast - pharmacology Life Sciences Medical Microbiology Microbiology Original Paper Pichia Pichia - drug effects Pichia - growth & development Plant Sciences Potassium phosphate Potassium phosphates Saccharomyces Saccharomyces - chemistry Saccharomyces - genetics Saccharomyces - metabolism Soil Science & Conservation Spoilage Urea Waste Products - analysis Waste treatment Wine Wine - microbiology Yeast Yeasts |
| title | Production of a novel killer toxin from Saccharomyces eubayanus using agro-industrial waste and its application against wine spoilage yeasts |
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