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Barcoding chemical modifications into nucleic acids improves drug stability in vivo
The efficacy of nucleic acid therapies can be limited by unwanted degradation. Chemical modifications are known to improve nucleic acid stability, but the (i) types, (ii) positions, and (iii) numbers of modifications all matter, making chemically optimizing nucleic acids a combinatorial problem. As...
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| Published in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2018-11, Vol.6 (44), p.7197-723 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c454t-a56094bd1976fd450b19cb79f0627cf9b41dc75d97773d59512481469b97be8a3 |
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| cites | cdi_FETCH-LOGICAL-c454t-a56094bd1976fd450b19cb79f0627cf9b41dc75d97773d59512481469b97be8a3 |
| container_end_page | 723 |
| container_issue | 44 |
| container_start_page | 7197 |
| container_title | Journal of materials chemistry. B, Materials for biology and medicine |
| container_volume | 6 |
| creator | Sago, Cory D Kalathoor, Sujay Fitzgerald, Jordan P Lando, Gwyneth N Djeddar, Naima Bryksin, Anton V Dahlman, James E |
| description | The efficacy of nucleic acid therapies can be limited by unwanted degradation. Chemical modifications are known to improve nucleic acid stability, but the (i) types, (ii) positions, and (iii) numbers of modifications all matter, making chemically optimizing nucleic acids a combinatorial problem. As a result,
in vivo
studies of nucleic acid stability are time consuming and expensive. We reasoned that DNA barcodes could simultaneously study how chemical modification patterns affect nucleic acid stability, saving time and resources. We confirmed that rationally designed DNA barcodes can elucidate the role of specific chemical modifications in serum,
in vitro
and
in vivo
; we also identified a modification pattern that enhanced stability. This approach to screening chemical modifications
in vivo
can efficiently optimize nucleic acid structure, which will improve biomaterial-based nucleic acid drugs.
The efficacy of nucleic acid therapies can be limited by unwanted degradation. |
| doi_str_mv | 10.1039/c8tb01642a |
| format | article |
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in vivo
studies of nucleic acid stability are time consuming and expensive. We reasoned that DNA barcodes could simultaneously study how chemical modification patterns affect nucleic acid stability, saving time and resources. We confirmed that rationally designed DNA barcodes can elucidate the role of specific chemical modifications in serum,
in vitro
and
in vivo
; we also identified a modification pattern that enhanced stability. This approach to screening chemical modifications
in vivo
can efficiently optimize nucleic acid structure, which will improve biomaterial-based nucleic acid drugs.
The efficacy of nucleic acid therapies can be limited by unwanted degradation.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/c8tb01642a</identifier><identifier>PMID: 30555697</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Acids ; Bar codes ; Biomaterials ; Biomedical materials ; Chemical modification ; Chemistry ; Combinatorial analysis ; Deoxyribonucleic acid ; DNA ; Gene sequencing ; In vivo methods and tests ; Nucleic acids ; Optimization ; Organic chemistry ; Stability</subject><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2018-11, Vol.6 (44), p.7197-723</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><rights>This journal is © The Royal Society of Chemistry 2018 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-a56094bd1976fd450b19cb79f0627cf9b41dc75d97773d59512481469b97be8a3</citedby><cites>FETCH-LOGICAL-c454t-a56094bd1976fd450b19cb79f0627cf9b41dc75d97773d59512481469b97be8a3</cites><orcidid>0000-0001-7580-436X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30555697$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sago, Cory D</creatorcontrib><creatorcontrib>Kalathoor, Sujay</creatorcontrib><creatorcontrib>Fitzgerald, Jordan P</creatorcontrib><creatorcontrib>Lando, Gwyneth N</creatorcontrib><creatorcontrib>Djeddar, Naima</creatorcontrib><creatorcontrib>Bryksin, Anton V</creatorcontrib><creatorcontrib>Dahlman, James E</creatorcontrib><title>Barcoding chemical modifications into nucleic acids improves drug stability in vivo</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><addtitle>J Mater Chem B</addtitle><description>The efficacy of nucleic acid therapies can be limited by unwanted degradation. Chemical modifications are known to improve nucleic acid stability, but the (i) types, (ii) positions, and (iii) numbers of modifications all matter, making chemically optimizing nucleic acids a combinatorial problem. As a result,
in vivo
studies of nucleic acid stability are time consuming and expensive. We reasoned that DNA barcodes could simultaneously study how chemical modification patterns affect nucleic acid stability, saving time and resources. We confirmed that rationally designed DNA barcodes can elucidate the role of specific chemical modifications in serum,
in vitro
and
in vivo
; we also identified a modification pattern that enhanced stability. This approach to screening chemical modifications
in vivo
can efficiently optimize nucleic acid structure, which will improve biomaterial-based nucleic acid drugs.
The efficacy of nucleic acid therapies can be limited by unwanted degradation.</description><subject>Acids</subject><subject>Bar codes</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Chemical modification</subject><subject>Chemistry</subject><subject>Combinatorial analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gene sequencing</subject><subject>In vivo methods and tests</subject><subject>Nucleic acids</subject><subject>Optimization</subject><subject>Organic chemistry</subject><subject>Stability</subject><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp90clLJDEUB-AwKKOol7mPlMxFhNaksl8EbdxA8KDC3EK2aiNVlTapavC_n2hrj3owlyzv4_HCD4BfCB4iiOWRFYOBiJFa_wCbNaRwwikSa6sz_LsBdnJ-hGUJxAQmP8EGhpRSJvkmuD3VyUYX-lllH3wXrG6rrtybchpC7HMV-iFW_WhbH2ylbXDlqZunuPC5cmmcVXnQJrRheC60WoRF3AbrjW6z33nbt8D9-dnd9HJyfXNxNT25nlhCyTDRlEFJjEOSs8YRCg2S1nDZQFZz20hDkLOcOsk5x45KimoiEGHSSG680HgLHC_7zkfTeWd9PyTdqnkKnU7PKuqgPlf68KBmcaFYjQWvaWmw_9YgxafR50F1IVvftrr3ccyqRpQzSqAUhf75Qh_jmPryvaIwrjGm5EUdLJVNMefkm9UwCKqXuNRU3J2-xnVS8O7H8Vf0PZwC9pYgZbuq_s9bzV1TzO_vDP4HDxulkA</recordid><startdate>20181128</startdate><enddate>20181128</enddate><creator>Sago, Cory D</creator><creator>Kalathoor, Sujay</creator><creator>Fitzgerald, Jordan P</creator><creator>Lando, Gwyneth N</creator><creator>Djeddar, Naima</creator><creator>Bryksin, Anton V</creator><creator>Dahlman, James E</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7580-436X</orcidid></search><sort><creationdate>20181128</creationdate><title>Barcoding chemical modifications into nucleic acids improves drug stability in vivo</title><author>Sago, Cory D ; 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B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sago, Cory D</au><au>Kalathoor, Sujay</au><au>Fitzgerald, Jordan P</au><au>Lando, Gwyneth N</au><au>Djeddar, Naima</au><au>Bryksin, Anton V</au><au>Dahlman, James E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Barcoding chemical modifications into nucleic acids improves drug stability in vivo</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><addtitle>J Mater Chem B</addtitle><date>2018-11-28</date><risdate>2018</risdate><volume>6</volume><issue>44</issue><spage>7197</spage><epage>723</epage><pages>7197-723</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The efficacy of nucleic acid therapies can be limited by unwanted degradation. Chemical modifications are known to improve nucleic acid stability, but the (i) types, (ii) positions, and (iii) numbers of modifications all matter, making chemically optimizing nucleic acids a combinatorial problem. As a result,
in vivo
studies of nucleic acid stability are time consuming and expensive. We reasoned that DNA barcodes could simultaneously study how chemical modification patterns affect nucleic acid stability, saving time and resources. We confirmed that rationally designed DNA barcodes can elucidate the role of specific chemical modifications in serum,
in vitro
and
in vivo
; we also identified a modification pattern that enhanced stability. This approach to screening chemical modifications
in vivo
can efficiently optimize nucleic acid structure, which will improve biomaterial-based nucleic acid drugs.
The efficacy of nucleic acid therapies can be limited by unwanted degradation.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>30555697</pmid><doi>10.1039/c8tb01642a</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-7580-436X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2018-11, Vol.6 (44), p.7197-723 |
| issn | 2050-750X 2050-7518 |
| language | eng |
| recordid | cdi_proquest_journals_2133233548 |
| source | Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list) |
| subjects | Acids Bar codes Biomaterials Biomedical materials Chemical modification Chemistry Combinatorial analysis Deoxyribonucleic acid DNA Gene sequencing In vivo methods and tests Nucleic acids Optimization Organic chemistry Stability |
| title | Barcoding chemical modifications into nucleic acids improves drug stability in vivo |
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