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Silver‐doped 58S bioactive glass as an anti‐Leishmania agent
Bioactive glasses (BG) incorporating antimicrobial agents can be effectively used against microorganisms. In this work, the in vitro effectiveness of silver‐doped 58S BG (BGAg) against Leishmania species was studied. BG, BGAg1, and BGAg2 belonging to the system 58SiO2∙(36‐x) CaO·6P2O5·xAg2O, where x...
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| Published in: | International journal of applied glass science 2018-01, Vol.9 (1), p.52-61 |
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| container_title | International journal of applied glass science |
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| creator | Pires, Emanuene Galdino Bonan, Roberta Ferreti Rocha, Ítalo Martins Gonçalves, Ingrid Morgana Fernandes Souza, Joelma Rodrigues Gonzales, Laura Helena Vega Silva Júnior, José Valter Joaquim Perez, Danyel Elias da Cruz Tavares, Paula Cristina Brígido Silva, Sydnei Magno da Alves‐Balvedi, Renata Pereira Goulart, Luiz Ricardo Medeiros, Eliton Souto Castellano, Lucio Roberto Bonan, Paulo Rogério Ferreti |
| description | Bioactive glasses (BG) incorporating antimicrobial agents can be effectively used against microorganisms. In this work, the in vitro effectiveness of silver‐doped 58S BG (BGAg) against Leishmania species was studied. BG, BGAg1, and BGAg2 belonging to the system 58SiO2∙(36‐x) CaO·6P2O5·xAg2O, where x=0, 1, and 2 mol.% Ag, were synthesized via sol‐gel, and characterized by scanning electron (SEM) and atomic force (AFM) microscopy, thermogravimetric analyses (TGA), X‐ray diffraction (XRD), Fourier‐transform infrared (FTIR), and surface‐enhanced Raman (Raman‐SERS) spectroscopy. Cytotoxicity was assessed in A549 lung adenocarcinoma cells. Leishmania amazonensis and Leishmania braziliensis cultures were exposed to all groups, and C57BL/6 macrophages were infected by over metacyclic form L. amazonensis under the exposure of BGAg particles. SEM and AFM images showed an irregular and network arranged surface. TGA, XRD, FTIR, and RAMAN‐SERS analyses confirmed silver inclusion within BG. None of the BG and BGAg presented toxicity. BGAg2 was effective in controlling promastigote forms under 150 and 300 μg/mL concentrations of both evaluated species. On macrophage invasion assay, BGAg2 presented reduction in metacyclic forms. For 72 hours, BGAg1 (150 μg/mL), BGAg1 (300 μg/mL), and BGAg2 in all concentrations were effective against intracellular infection. BGAg could be used as an alternative or complimentary agent to current chemotherapy. |
| doi_str_mv | 10.1111/ijag.12285 |
| format | article |
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In this work, the in vitro effectiveness of silver‐doped 58S BG (BGAg) against Leishmania species was studied. BG, BGAg1, and BGAg2 belonging to the system 58SiO2∙(36‐x) CaO·6P2O5·xAg2O, where x=0, 1, and 2 mol.% Ag, were synthesized via sol‐gel, and characterized by scanning electron (SEM) and atomic force (AFM) microscopy, thermogravimetric analyses (TGA), X‐ray diffraction (XRD), Fourier‐transform infrared (FTIR), and surface‐enhanced Raman (Raman‐SERS) spectroscopy. Cytotoxicity was assessed in A549 lung adenocarcinoma cells. Leishmania amazonensis and Leishmania braziliensis cultures were exposed to all groups, and C57BL/6 macrophages were infected by over metacyclic form L. amazonensis under the exposure of BGAg particles. SEM and AFM images showed an irregular and network arranged surface. TGA, XRD, FTIR, and RAMAN‐SERS analyses confirmed silver inclusion within BG. None of the BG and BGAg presented toxicity. BGAg2 was effective in controlling promastigote forms under 150 and 300 μg/mL concentrations of both evaluated species. On macrophage invasion assay, BGAg2 presented reduction in metacyclic forms. For 72 hours, BGAg1 (150 μg/mL), BGAg1 (300 μg/mL), and BGAg2 in all concentrations were effective against intracellular infection. BGAg could be used as an alternative or complimentary agent to current chemotherapy.</description><identifier>ISSN: 2041-1286</identifier><identifier>EISSN: 2041-1294</identifier><identifier>DOI: 10.1111/ijag.12285</identifier><language>eng</language><publisher>Westerville: Wiley Subscription Services, Inc</publisher><subject>antiparasitic agent ; Atomic force microscopy ; Biocompatibility ; Biological activity ; Chemotherapy ; Fourier transforms ; Infrared analysis ; Infrared spectroscopy ; leishmaniasis ; Macrophages ; Microorganisms ; Silver ; silver‐doped bioactive glass ; Sol-gel processes ; sol‐gel ; Toxicity ; X-ray diffraction</subject><ispartof>International journal of applied glass science, 2018-01, Vol.9 (1), p.52-61</ispartof><rights>2017 The American Ceramic Society and Wiley Periodicals, Inc</rights><rights>2018 American Ceramic Society and Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3715-e4d6a02beea1204c4585a502ca737dbadfbfb7320d957e3be6ef9fe197364e863</citedby><cites>FETCH-LOGICAL-c3715-e4d6a02beea1204c4585a502ca737dbadfbfb7320d957e3be6ef9fe197364e863</cites><orcidid>0000-0002-4449-4343</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fijag.12285$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fijag.12285$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,50923,51032</link.rule.ids></links><search><creatorcontrib>Pires, Emanuene Galdino</creatorcontrib><creatorcontrib>Bonan, Roberta Ferreti</creatorcontrib><creatorcontrib>Rocha, Ítalo Martins</creatorcontrib><creatorcontrib>Gonçalves, Ingrid Morgana Fernandes</creatorcontrib><creatorcontrib>Souza, Joelma Rodrigues</creatorcontrib><creatorcontrib>Gonzales, Laura Helena Vega</creatorcontrib><creatorcontrib>Silva Júnior, José Valter Joaquim</creatorcontrib><creatorcontrib>Perez, Danyel Elias da Cruz</creatorcontrib><creatorcontrib>Tavares, Paula Cristina Brígido</creatorcontrib><creatorcontrib>Silva, Sydnei Magno da</creatorcontrib><creatorcontrib>Alves‐Balvedi, Renata Pereira</creatorcontrib><creatorcontrib>Goulart, Luiz Ricardo</creatorcontrib><creatorcontrib>Medeiros, Eliton Souto</creatorcontrib><creatorcontrib>Castellano, Lucio Roberto</creatorcontrib><creatorcontrib>Bonan, Paulo Rogério Ferreti</creatorcontrib><title>Silver‐doped 58S bioactive glass as an anti‐Leishmania agent</title><title>International journal of applied glass science</title><description>Bioactive glasses (BG) incorporating antimicrobial agents can be effectively used against microorganisms. In this work, the in vitro effectiveness of silver‐doped 58S BG (BGAg) against Leishmania species was studied. BG, BGAg1, and BGAg2 belonging to the system 58SiO2∙(36‐x) CaO·6P2O5·xAg2O, where x=0, 1, and 2 mol.% Ag, were synthesized via sol‐gel, and characterized by scanning electron (SEM) and atomic force (AFM) microscopy, thermogravimetric analyses (TGA), X‐ray diffraction (XRD), Fourier‐transform infrared (FTIR), and surface‐enhanced Raman (Raman‐SERS) spectroscopy. Cytotoxicity was assessed in A549 lung adenocarcinoma cells. Leishmania amazonensis and Leishmania braziliensis cultures were exposed to all groups, and C57BL/6 macrophages were infected by over metacyclic form L. amazonensis under the exposure of BGAg particles. SEM and AFM images showed an irregular and network arranged surface. TGA, XRD, FTIR, and RAMAN‐SERS analyses confirmed silver inclusion within BG. None of the BG and BGAg presented toxicity. BGAg2 was effective in controlling promastigote forms under 150 and 300 μg/mL concentrations of both evaluated species. On macrophage invasion assay, BGAg2 presented reduction in metacyclic forms. For 72 hours, BGAg1 (150 μg/mL), BGAg1 (300 μg/mL), and BGAg2 in all concentrations were effective against intracellular infection. BGAg could be used as an alternative or complimentary agent to current chemotherapy.</description><subject>antiparasitic agent</subject><subject>Atomic force microscopy</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Chemotherapy</subject><subject>Fourier transforms</subject><subject>Infrared analysis</subject><subject>Infrared spectroscopy</subject><subject>leishmaniasis</subject><subject>Macrophages</subject><subject>Microorganisms</subject><subject>Silver</subject><subject>silver‐doped bioactive glass</subject><subject>Sol-gel processes</subject><subject>sol‐gel</subject><subject>Toxicity</subject><subject>X-ray diffraction</subject><issn>2041-1286</issn><issn>2041-1294</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kM1Kw0AQxxdRsNRefIKANyF1P7LJ5mYpWisFD9XzMkkmcUua1N200puP4DP6JG6MeHT4w8zhN19_Qi4ZnTIfN2YD1ZRxruQJGXEasZDxNDr9q1V8TibObagPoVScqhG5XZv6gPbr47Nod1gEUq2DzLSQd-aAQVWDcwF4NV6d8dgKjXvdQmMggAqb7oKclVA7nPzmMXm5v3ueP4Srp8VyPluFuUiYDDEqYqA8QwTmz8kjqSRIynNIRFJkUJRZmSWC0yKVCYoMYyzTElmaiDhCFYsxuRrm7mz7tkfX6U27t41fqT1E_TtS9tT1QOW2dc5iqXfWbMEeNaO6N0n3JukfkzzMBvjd1Hj8h9TLx9li6PkGrI1qKQ</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Pires, Emanuene Galdino</creator><creator>Bonan, Roberta Ferreti</creator><creator>Rocha, Ítalo Martins</creator><creator>Gonçalves, Ingrid Morgana Fernandes</creator><creator>Souza, Joelma Rodrigues</creator><creator>Gonzales, Laura Helena Vega</creator><creator>Silva Júnior, José Valter Joaquim</creator><creator>Perez, Danyel Elias da Cruz</creator><creator>Tavares, Paula Cristina Brígido</creator><creator>Silva, Sydnei Magno da</creator><creator>Alves‐Balvedi, Renata Pereira</creator><creator>Goulart, Luiz Ricardo</creator><creator>Medeiros, Eliton Souto</creator><creator>Castellano, Lucio Roberto</creator><creator>Bonan, Paulo Rogério Ferreti</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QQ</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0002-4449-4343</orcidid></search><sort><creationdate>201801</creationdate><title>Silver‐doped 58S bioactive glass as an anti‐Leishmania agent</title><author>Pires, Emanuene Galdino ; Bonan, Roberta Ferreti ; Rocha, Ítalo Martins ; Gonçalves, Ingrid Morgana Fernandes ; Souza, Joelma Rodrigues ; Gonzales, Laura Helena Vega ; Silva Júnior, José Valter Joaquim ; Perez, Danyel Elias da Cruz ; Tavares, Paula Cristina Brígido ; Silva, Sydnei Magno da ; Alves‐Balvedi, Renata Pereira ; Goulart, Luiz Ricardo ; Medeiros, Eliton Souto ; Castellano, Lucio Roberto ; Bonan, Paulo Rogério Ferreti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3715-e4d6a02beea1204c4585a502ca737dbadfbfb7320d957e3be6ef9fe197364e863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>antiparasitic agent</topic><topic>Atomic force microscopy</topic><topic>Biocompatibility</topic><topic>Biological activity</topic><topic>Chemotherapy</topic><topic>Fourier transforms</topic><topic>Infrared analysis</topic><topic>Infrared spectroscopy</topic><topic>leishmaniasis</topic><topic>Macrophages</topic><topic>Microorganisms</topic><topic>Silver</topic><topic>silver‐doped bioactive glass</topic><topic>Sol-gel processes</topic><topic>sol‐gel</topic><topic>Toxicity</topic><topic>X-ray diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pires, Emanuene Galdino</creatorcontrib><creatorcontrib>Bonan, Roberta Ferreti</creatorcontrib><creatorcontrib>Rocha, Ítalo Martins</creatorcontrib><creatorcontrib>Gonçalves, Ingrid Morgana Fernandes</creatorcontrib><creatorcontrib>Souza, Joelma Rodrigues</creatorcontrib><creatorcontrib>Gonzales, Laura Helena Vega</creatorcontrib><creatorcontrib>Silva Júnior, José Valter Joaquim</creatorcontrib><creatorcontrib>Perez, Danyel Elias da Cruz</creatorcontrib><creatorcontrib>Tavares, Paula Cristina Brígido</creatorcontrib><creatorcontrib>Silva, Sydnei Magno da</creatorcontrib><creatorcontrib>Alves‐Balvedi, Renata Pereira</creatorcontrib><creatorcontrib>Goulart, Luiz Ricardo</creatorcontrib><creatorcontrib>Medeiros, Eliton Souto</creatorcontrib><creatorcontrib>Castellano, Lucio Roberto</creatorcontrib><creatorcontrib>Bonan, Paulo Rogério Ferreti</creatorcontrib><collection>CrossRef</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>International journal of applied glass science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pires, Emanuene Galdino</au><au>Bonan, Roberta Ferreti</au><au>Rocha, Ítalo Martins</au><au>Gonçalves, Ingrid Morgana Fernandes</au><au>Souza, Joelma Rodrigues</au><au>Gonzales, Laura Helena Vega</au><au>Silva Júnior, José Valter Joaquim</au><au>Perez, Danyel Elias da Cruz</au><au>Tavares, Paula Cristina Brígido</au><au>Silva, Sydnei Magno da</au><au>Alves‐Balvedi, Renata Pereira</au><au>Goulart, Luiz Ricardo</au><au>Medeiros, Eliton Souto</au><au>Castellano, Lucio Roberto</au><au>Bonan, Paulo Rogério Ferreti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silver‐doped 58S bioactive glass as an anti‐Leishmania agent</atitle><jtitle>International journal of applied glass science</jtitle><date>2018-01</date><risdate>2018</risdate><volume>9</volume><issue>1</issue><spage>52</spage><epage>61</epage><pages>52-61</pages><issn>2041-1286</issn><eissn>2041-1294</eissn><abstract>Bioactive glasses (BG) incorporating antimicrobial agents can be effectively used against microorganisms. In this work, the in vitro effectiveness of silver‐doped 58S BG (BGAg) against Leishmania species was studied. BG, BGAg1, and BGAg2 belonging to the system 58SiO2∙(36‐x) CaO·6P2O5·xAg2O, where x=0, 1, and 2 mol.% Ag, were synthesized via sol‐gel, and characterized by scanning electron (SEM) and atomic force (AFM) microscopy, thermogravimetric analyses (TGA), X‐ray diffraction (XRD), Fourier‐transform infrared (FTIR), and surface‐enhanced Raman (Raman‐SERS) spectroscopy. Cytotoxicity was assessed in A549 lung adenocarcinoma cells. Leishmania amazonensis and Leishmania braziliensis cultures were exposed to all groups, and C57BL/6 macrophages were infected by over metacyclic form L. amazonensis under the exposure of BGAg particles. SEM and AFM images showed an irregular and network arranged surface. TGA, XRD, FTIR, and RAMAN‐SERS analyses confirmed silver inclusion within BG. None of the BG and BGAg presented toxicity. BGAg2 was effective in controlling promastigote forms under 150 and 300 μg/mL concentrations of both evaluated species. On macrophage invasion assay, BGAg2 presented reduction in metacyclic forms. For 72 hours, BGAg1 (150 μg/mL), BGAg1 (300 μg/mL), and BGAg2 in all concentrations were effective against intracellular infection. BGAg could be used as an alternative or complimentary agent to current chemotherapy.</abstract><cop>Westerville</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/ijag.12285</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4449-4343</orcidid></addata></record> |
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| subjects | antiparasitic agent Atomic force microscopy Biocompatibility Biological activity Chemotherapy Fourier transforms Infrared analysis Infrared spectroscopy leishmaniasis Macrophages Microorganisms Silver silver‐doped bioactive glass Sol-gel processes sol‐gel Toxicity X-ray diffraction |
| title | Silver‐doped 58S bioactive glass as an anti‐Leishmania agent |
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