Loading…
Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment
We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comp...
Saved in:
| Published in: | Zygote (Cambridge) 2017-08, Vol.25 (4), p.453-461 |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3 |
| container_end_page | 461 |
| container_issue | 4 |
| container_start_page | 453 |
| container_title | Zygote (Cambridge) |
| container_volume | 25 |
| creator | Li, Xiao-Chen Guo, Qing Zhu, Hai-Ying Jin, Long Zhang, Yu-Chen Zhang, Guang-Lei Xing, Xiao-Xu Xuan, Mei-Fu Luo, Qi-Rong Luo, Zhao-Bo Wang, Jun-Xia Cui, Cheng-Du Li, Wen-Xue Cui, Zheng-Yun Yin, Xi-Jun Kang, Jin-Dan |
| description | We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P < 0.05). The blastocyst formation rate was higher in the group treated with AZD5438 for 4 h than in the groups treated with AZD5438 for 2 or 6 h (42.8% vs. 38.6% and 37.2%, respectively; P > 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype. The blastocyst formation rate of PA and somatic cell nuclear transfer (SCNT) embryos was similar between oocytes activated by an EP and treated with 2 mM 6-dimethylaminopurine for 4 h and those activated by an EP and treated with 10 µM AZD5438 for 4 h (11.11% vs. 13.40%, P > 0.05). In addition, the level of maturation-promoting factor (MPF) was significantly decreased in oocytes activated by an EP and treated with 10 µM AZD5438 for 4 h. Finally, the mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) and pluripotency-related genes (Oct4, Nanog, and Sox2) were checked by RT-PCR; however, there were no differences between the AZD5438-treated and non-treated control groups. Our results demonstrate that porcine oocyte activation via an EP in combination with AZD5438 treatment can lead to a high blastocyst formation rate in PA and SCNT experiments. |
| doi_str_mv | 10.1017/S0967199417000272 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1933242423</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cupid>10_1017_S0967199417000272</cupid><sourcerecordid>1933242423</sourcerecordid><originalsourceid>FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3</originalsourceid><addsrcrecordid>eNp1kEFr3DAQhUVJaTbb_oBegiBnp5IlW9IxpGlTCLTQ9tKLGUvjxMGWtpIc2Ht-eLXd3VIoZQ4D8977Bh4hbzm75Iyrd1-ZaRU3RnLFGKtV_YKsuGxNpZVgJ2S1k6udfkrOUnosHqWMfEVOa614LZRckecvEPMD-nCPHvNoKdg8PkEeg6fgHU1hht3Z4jRRv9gJIdIcwacBIw0D3YRoR480BLvNmI55dLTfFgLFCW2OhbBZpoS_mVc_3jdS6IJByDP6_Jq8HKCobw57Tb5_uPl2fVvdff746frqrrJCiVw1rjEG-tYI3QxKSqahBhCgnOBcOGQtV2Ca1g29q41UemiZFhpb1NJq4cSaXOy5mxh-Lphy9xiW6MvLjhshallGFBffu2wMKUUcuk0cZ4jbjrNu13v3T-8lc34gL_2M7k_iWHQxiAMU5j6O7h7_-v1f7C8PDI2R</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1933242423</pqid></control><display><type>article</type><title>Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment</title><source>Cambridge University Press</source><creator>Li, Xiao-Chen ; Guo, Qing ; Zhu, Hai-Ying ; Jin, Long ; Zhang, Yu-Chen ; Zhang, Guang-Lei ; Xing, Xiao-Xu ; Xuan, Mei-Fu ; Luo, Qi-Rong ; Luo, Zhao-Bo ; Wang, Jun-Xia ; Cui, Cheng-Du ; Li, Wen-Xue ; Cui, Zheng-Yun ; Yin, Xi-Jun ; Kang, Jin-Dan</creator><creatorcontrib>Li, Xiao-Chen ; Guo, Qing ; Zhu, Hai-Ying ; Jin, Long ; Zhang, Yu-Chen ; Zhang, Guang-Lei ; Xing, Xiao-Xu ; Xuan, Mei-Fu ; Luo, Qi-Rong ; Luo, Zhao-Bo ; Wang, Jun-Xia ; Cui, Cheng-Du ; Li, Wen-Xue ; Cui, Zheng-Yun ; Yin, Xi-Jun ; Kang, Jin-Dan</creatorcontrib><description>We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P < 0.05). The blastocyst formation rate was higher in the group treated with AZD5438 for 4 h than in the groups treated with AZD5438 for 2 or 6 h (42.8% vs. 38.6% and 37.2%, respectively; P > 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype. The blastocyst formation rate of PA and somatic cell nuclear transfer (SCNT) embryos was similar between oocytes activated by an EP and treated with 2 mM 6-dimethylaminopurine for 4 h and those activated by an EP and treated with 10 µM AZD5438 for 4 h (11.11% vs. 13.40%, P > 0.05). In addition, the level of maturation-promoting factor (MPF) was significantly decreased in oocytes activated by an EP and treated with 10 µM AZD5438 for 4 h. Finally, the mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) and pluripotency-related genes (Oct4, Nanog, and Sox2) were checked by RT-PCR; however, there were no differences between the AZD5438-treated and non-treated control groups. Our results demonstrate that porcine oocyte activation via an EP in combination with AZD5438 treatment can lead to a high blastocyst formation rate in PA and SCNT experiments.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199417000272</identifier><identifier>PMID: 28712374</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Adenine - analogs & derivatives ; Adenine - pharmacology ; Animals ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - genetics ; Bcl-2 protein ; Blastocyst - physiology ; Blastocysts ; Cell activation ; Cell division ; Chromosomes ; Electric Stimulation ; Embryos ; Female ; Gene expression ; Gene Expression Regulation, Developmental ; Genes ; GPI-Linked Proteins - metabolism ; Imidazoles - administration & dosage ; Imidazoles - pharmacology ; In vitro methods and tests ; In Vitro Oocyte Maturation Techniques - methods ; Karyotyping ; Kinases ; Laboratories ; Maturation ; Maturation-promoting factor ; Nuclear transfer ; Nuclear Transfer Techniques ; Oct-4 protein ; Oocytes ; Oocytes - drug effects ; Oocytes - physiology ; Parthenogenesis - drug effects ; Parthenogenesis - physiology ; Phosphorylation ; Pluripotency ; Polymerase chain reaction ; Polyvinyl alcohol ; Proteins ; Pyrimidines - administration & dosage ; Pyrimidines - pharmacology ; Rodents ; Somatic cell nuclear transfer ; Stem cells ; Studies ; Swine ; Transgenic animals</subject><ispartof>Zygote (Cambridge), 2017-08, Vol.25 (4), p.453-461</ispartof><rights>Copyright © Cambridge University Press 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3</citedby><cites>FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0967199417000272/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>315,786,790,27957,27958,73317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28712374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiao-Chen</creatorcontrib><creatorcontrib>Guo, Qing</creatorcontrib><creatorcontrib>Zhu, Hai-Ying</creatorcontrib><creatorcontrib>Jin, Long</creatorcontrib><creatorcontrib>Zhang, Yu-Chen</creatorcontrib><creatorcontrib>Zhang, Guang-Lei</creatorcontrib><creatorcontrib>Xing, Xiao-Xu</creatorcontrib><creatorcontrib>Xuan, Mei-Fu</creatorcontrib><creatorcontrib>Luo, Qi-Rong</creatorcontrib><creatorcontrib>Luo, Zhao-Bo</creatorcontrib><creatorcontrib>Wang, Jun-Xia</creatorcontrib><creatorcontrib>Cui, Cheng-Du</creatorcontrib><creatorcontrib>Li, Wen-Xue</creatorcontrib><creatorcontrib>Cui, Zheng-Yun</creatorcontrib><creatorcontrib>Yin, Xi-Jun</creatorcontrib><creatorcontrib>Kang, Jin-Dan</creatorcontrib><title>Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P < 0.05). The blastocyst formation rate was higher in the group treated with AZD5438 for 4 h than in the groups treated with AZD5438 for 2 or 6 h (42.8% vs. 38.6% and 37.2%, respectively; P > 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype. The blastocyst formation rate of PA and somatic cell nuclear transfer (SCNT) embryos was similar between oocytes activated by an EP and treated with 2 mM 6-dimethylaminopurine for 4 h and those activated by an EP and treated with 10 µM AZD5438 for 4 h (11.11% vs. 13.40%, P > 0.05). In addition, the level of maturation-promoting factor (MPF) was significantly decreased in oocytes activated by an EP and treated with 10 µM AZD5438 for 4 h. Finally, the mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) and pluripotency-related genes (Oct4, Nanog, and Sox2) were checked by RT-PCR; however, there were no differences between the AZD5438-treated and non-treated control groups. Our results demonstrate that porcine oocyte activation via an EP in combination with AZD5438 treatment can lead to a high blastocyst formation rate in PA and SCNT experiments.</description><subject>Adenine - analogs & derivatives</subject><subject>Adenine - pharmacology</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Bcl-2 protein</subject><subject>Blastocyst - physiology</subject><subject>Blastocysts</subject><subject>Cell activation</subject><subject>Cell division</subject><subject>Chromosomes</subject><subject>Electric Stimulation</subject><subject>Embryos</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>GPI-Linked Proteins - metabolism</subject><subject>Imidazoles - administration & dosage</subject><subject>Imidazoles - pharmacology</subject><subject>In vitro methods and tests</subject><subject>In Vitro Oocyte Maturation Techniques - methods</subject><subject>Karyotyping</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Maturation</subject><subject>Maturation-promoting factor</subject><subject>Nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>Oct-4 protein</subject><subject>Oocytes</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Parthenogenesis - drug effects</subject><subject>Parthenogenesis - physiology</subject><subject>Phosphorylation</subject><subject>Pluripotency</subject><subject>Polymerase chain reaction</subject><subject>Polyvinyl alcohol</subject><subject>Proteins</subject><subject>Pyrimidines - administration & dosage</subject><subject>Pyrimidines - pharmacology</subject><subject>Rodents</subject><subject>Somatic cell nuclear transfer</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Swine</subject><subject>Transgenic animals</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kEFr3DAQhUVJaTbb_oBegiBnp5IlW9IxpGlTCLTQ9tKLGUvjxMGWtpIc2Ht-eLXd3VIoZQ4D8977Bh4hbzm75Iyrd1-ZaRU3RnLFGKtV_YKsuGxNpZVgJ2S1k6udfkrOUnosHqWMfEVOa614LZRckecvEPMD-nCPHvNoKdg8PkEeg6fgHU1hht3Z4jRRv9gJIdIcwacBIw0D3YRoR480BLvNmI55dLTfFgLFCW2OhbBZpoS_mVc_3jdS6IJByDP6_Jq8HKCobw57Tb5_uPl2fVvdff746frqrrJCiVw1rjEG-tYI3QxKSqahBhCgnOBcOGQtV2Ca1g29q41UemiZFhpb1NJq4cSaXOy5mxh-Lphy9xiW6MvLjhshallGFBffu2wMKUUcuk0cZ4jbjrNu13v3T-8lc34gL_2M7k_iWHQxiAMU5j6O7h7_-v1f7C8PDI2R</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Li, Xiao-Chen</creator><creator>Guo, Qing</creator><creator>Zhu, Hai-Ying</creator><creator>Jin, Long</creator><creator>Zhang, Yu-Chen</creator><creator>Zhang, Guang-Lei</creator><creator>Xing, Xiao-Xu</creator><creator>Xuan, Mei-Fu</creator><creator>Luo, Qi-Rong</creator><creator>Luo, Zhao-Bo</creator><creator>Wang, Jun-Xia</creator><creator>Cui, Cheng-Du</creator><creator>Li, Wen-Xue</creator><creator>Cui, Zheng-Yun</creator><creator>Yin, Xi-Jun</creator><creator>Kang, Jin-Dan</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>201708</creationdate><title>Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment</title><author>Li, Xiao-Chen ; Guo, Qing ; Zhu, Hai-Ying ; Jin, Long ; Zhang, Yu-Chen ; Zhang, Guang-Lei ; Xing, Xiao-Xu ; Xuan, Mei-Fu ; Luo, Qi-Rong ; Luo, Zhao-Bo ; Wang, Jun-Xia ; Cui, Cheng-Du ; Li, Wen-Xue ; Cui, Zheng-Yun ; Yin, Xi-Jun ; Kang, Jin-Dan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenine - analogs & derivatives</topic><topic>Adenine - pharmacology</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Bcl-2 protein</topic><topic>Blastocyst - physiology</topic><topic>Blastocysts</topic><topic>Cell activation</topic><topic>Cell division</topic><topic>Chromosomes</topic><topic>Electric Stimulation</topic><topic>Embryos</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes</topic><topic>GPI-Linked Proteins - metabolism</topic><topic>Imidazoles - administration & dosage</topic><topic>Imidazoles - pharmacology</topic><topic>In vitro methods and tests</topic><topic>In Vitro Oocyte Maturation Techniques - methods</topic><topic>Karyotyping</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Maturation</topic><topic>Maturation-promoting factor</topic><topic>Nuclear transfer</topic><topic>Nuclear Transfer Techniques</topic><topic>Oct-4 protein</topic><topic>Oocytes</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Parthenogenesis - drug effects</topic><topic>Parthenogenesis - physiology</topic><topic>Phosphorylation</topic><topic>Pluripotency</topic><topic>Polymerase chain reaction</topic><topic>Polyvinyl alcohol</topic><topic>Proteins</topic><topic>Pyrimidines - administration & dosage</topic><topic>Pyrimidines - pharmacology</topic><topic>Rodents</topic><topic>Somatic cell nuclear transfer</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Swine</topic><topic>Transgenic animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiao-Chen</creatorcontrib><creatorcontrib>Guo, Qing</creatorcontrib><creatorcontrib>Zhu, Hai-Ying</creatorcontrib><creatorcontrib>Jin, Long</creatorcontrib><creatorcontrib>Zhang, Yu-Chen</creatorcontrib><creatorcontrib>Zhang, Guang-Lei</creatorcontrib><creatorcontrib>Xing, Xiao-Xu</creatorcontrib><creatorcontrib>Xuan, Mei-Fu</creatorcontrib><creatorcontrib>Luo, Qi-Rong</creatorcontrib><creatorcontrib>Luo, Zhao-Bo</creatorcontrib><creatorcontrib>Wang, Jun-Xia</creatorcontrib><creatorcontrib>Cui, Cheng-Du</creatorcontrib><creatorcontrib>Li, Wen-Xue</creatorcontrib><creatorcontrib>Cui, Zheng-Yun</creatorcontrib><creatorcontrib>Yin, Xi-Jun</creatorcontrib><creatorcontrib>Kang, Jin-Dan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiao-Chen</au><au>Guo, Qing</au><au>Zhu, Hai-Ying</au><au>Jin, Long</au><au>Zhang, Yu-Chen</au><au>Zhang, Guang-Lei</au><au>Xing, Xiao-Xu</au><au>Xuan, Mei-Fu</au><au>Luo, Qi-Rong</au><au>Luo, Zhao-Bo</au><au>Wang, Jun-Xia</au><au>Cui, Cheng-Du</au><au>Li, Wen-Xue</au><au>Cui, Zheng-Yun</au><au>Yin, Xi-Jun</au><au>Kang, Jin-Dan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2017-08</date><risdate>2017</risdate><volume>25</volume><issue>4</issue><spage>453</spage><epage>461</epage><pages>453-461</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>We examined the in vitro developmental competence of parthenogenetic activation (PA) oocytes activated by an electric pulse (EP) and treated with various concentrations of AZD5438 for 4 h. Treatment with 10 µM AZD5438 for 4 h significantly improved the blastocyst formation rate of PA oocytes in comparison with 0, 20, or 50 µM AZD5438 treatment (46.4% vs. 34.5%, 32.3%, and 24.0%, respectively; P < 0.05). The blastocyst formation rate was higher in the group treated with AZD5438 for 4 h than in the groups treated with AZD5438 for 2 or 6 h (42.8% vs. 38.6% and 37.2%, respectively; P > 0.05). Furthermore, 66.67% of blastocysts derived from these AZD5438-treated PA oocytes had a diploid karyotype. The blastocyst formation rate of PA and somatic cell nuclear transfer (SCNT) embryos was similar between oocytes activated by an EP and treated with 2 mM 6-dimethylaminopurine for 4 h and those activated by an EP and treated with 10 µM AZD5438 for 4 h (11.11% vs. 13.40%, P > 0.05). In addition, the level of maturation-promoting factor (MPF) was significantly decreased in oocytes activated by an EP and treated with 10 µM AZD5438 for 4 h. Finally, the mRNA expression levels of apoptosis-related genes (Bax and Bcl-2) and pluripotency-related genes (Oct4, Nanog, and Sox2) were checked by RT-PCR; however, there were no differences between the AZD5438-treated and non-treated control groups. Our results demonstrate that porcine oocyte activation via an EP in combination with AZD5438 treatment can lead to a high blastocyst formation rate in PA and SCNT experiments.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>28712374</pmid><doi>10.1017/S0967199417000272</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0967-1994 |
| ispartof | Zygote (Cambridge), 2017-08, Vol.25 (4), p.453-461 |
| issn | 0967-1994 1469-8730 |
| language | eng |
| recordid | cdi_proquest_journals_1933242423 |
| source | Cambridge University Press |
| subjects | Adenine - analogs & derivatives Adenine - pharmacology Animals Apoptosis Apoptosis - drug effects Apoptosis - genetics Bcl-2 protein Blastocyst - physiology Blastocysts Cell activation Cell division Chromosomes Electric Stimulation Embryos Female Gene expression Gene Expression Regulation, Developmental Genes GPI-Linked Proteins - metabolism Imidazoles - administration & dosage Imidazoles - pharmacology In vitro methods and tests In Vitro Oocyte Maturation Techniques - methods Karyotyping Kinases Laboratories Maturation Maturation-promoting factor Nuclear transfer Nuclear Transfer Techniques Oct-4 protein Oocytes Oocytes - drug effects Oocytes - physiology Parthenogenesis - drug effects Parthenogenesis - physiology Phosphorylation Pluripotency Polymerase chain reaction Polyvinyl alcohol Proteins Pyrimidines - administration & dosage Pyrimidines - pharmacology Rodents Somatic cell nuclear transfer Stem cells Studies Swine Transgenic animals |
| title | Parthenogenetic activation and somatic cell nuclear transfer of porcine oocytes activated by an electric pulse and AZD5438 treatment |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T20%3A02%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Parthenogenetic%20activation%20and%20somatic%20cell%20nuclear%20transfer%20of%20porcine%20oocytes%20activated%20by%20an%20electric%20pulse%20and%20AZD5438%20treatment&rft.jtitle=Zygote%20(Cambridge)&rft.au=Li,%20Xiao-Chen&rft.date=2017-08&rft.volume=25&rft.issue=4&rft.spage=453&rft.epage=461&rft.pages=453-461&rft.issn=0967-1994&rft.eissn=1469-8730&rft_id=info:doi/10.1017/S0967199417000272&rft_dat=%3Cproquest_cross%3E1933242423%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c373t-5d599ab69385f74408a2aa3a7d3113de0617a956dfbd29478f60838e6e84c83d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1933242423&rft_id=info:pmid/28712374&rft_cupid=10_1017_S0967199417000272&rfr_iscdi=true |