Synergistic effects of abiE or abiF from pNP40 when cloned in combination with abiD from pBF61

Two fragments conferring partial phage resistance were located on plasmid pNP40 from Lactococcus lactis ssp. lactis biovar diacetylactis DRC3 and cloned. A 2.3-kb PstI fragment from pNP40 containing abiF conferred partial phage resistance to prolate-headed phage c2, and a 4.8-kb PstI fragment of pNP...

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Bibliographic Details
Published in:Journal of dairy science 1998-02, Vol.81 (2), p.362-368
Main Authors: McLandsborough, L.A. (University of Minnesota, St. Paul.), Sechaud, L, McKay, L.L
Format: Article
Language:English
Subjects:
BACTERIOPHAGES
Biological and medical sciences
BIOLOGICAL DIFFERENCES
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Food industries
Fundamental and applied biological sciences. Psychology
GENETIC RESISTANCE
Genetics
LACTOCOCCUS LACTIS
LACTOCOCCUS LACTIS SUBSP. LACTIS
Milk and cheese industries. Ice creams
Mission oriented research
PLASMIDS
STRAIN DIFFERENCES
SYNERGISM
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Summary:Two fragments conferring partial phage resistance were located on plasmid pNP40 from Lactococcus lactis ssp. lactis biovar diacetylactis DRC3 and cloned. A 2.3-kb PstI fragment from pNP40 containing abiF conferred partial phage resistance to prolate-headed phage c2, and a 4.8-kb PstI fragment of pNP40 containing abiE conferred partial phage resistance to small isometric-headed phage sk1. When each of the two fragments was cloned individually into a plasmid containing the abortive phage infection gene abiD from L. lactis ssp. lactis KR5, phage resistance was enhanced. When cloned with abiD, the 2.3-kb PstI fragment enhanced the resistance against prolate-headed phages, as was indicated by a 400-fold decrease in the efficiency of plating compared with that of abiD alone. When the 4.8-kb PstI fragment of pNP40 was cloned with abiD, resistance to small isometric-headed phages was enhanced, as was indicated by a greater than 50-fold decrease in efficiency of plating compared with that of abiD alone. The 4.8-kb PstI fragment of pNP40 cloned with abiD showed a large decrease (500- to 1000-fold) in efficiency of plating against prolate-headed phages, even though the 4.8-kb PstI fragment of pNP40 by itself conferred no resistance to the prolate-headed phages
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(98)75585-7