Conformational dynamics is more important than helical propensity for the folding of the all [alpha]-helical protein Im7

Im7 folds via an on-pathway intermediate that contains three of the four native [alpha]-helices. The missing helix, helix III, is the shortest and its failure to be formed until late in the pathway is related to frustration in the structure. Im7H3M3, a 94-residue variant of the 87-residue Im7 in whi...

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Bibliographic Details
Published in:Protein science 2013-12, Vol.22 (12), p.1722
Main Authors: Figueiredo, Angelo Miguel, Whittaker, Sara B-M, Knowling, Stuart E, Radford, Sheena E, Moore, Geoffrey R
Format: Article
Language:English
Subjects:
Biophysics
Protein folding
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Summary:Im7 folds via an on-pathway intermediate that contains three of the four native [alpha]-helices. The missing helix, helix III, is the shortest and its failure to be formed until late in the pathway is related to frustration in the structure. Im7H3M3, a 94-residue variant of the 87-residue Im7 in which helix III is the longest of the four native helices, also folds via an intermediate. To investigate the structural basis for this we calculated the frustration in the structure of Im7H3M3 and used NMR to investigate its dynamics. We found that the native state of Im7H3M3 is highly frustrated and in equilibrium with an intermediate state that lacks helix III, similar to Im7. Model-free analysis identified residues with chemical exchange contributions to their relaxation that aligned with the residues predicted to have highly frustrated interactions, also like Im7. Finally, we determined properties of urea-denatured Im7H3M3 and identified four clusters of interacting residues that corresponded to the [alpha]-helices of the native protein. In Im7 the cluster sizes were related to the lengths of the [alpha]-helices with cluster III being the smallest but in Im7H3M3 cluster III was also the smallest, despite this region forming the longest helix in the native state. These results suggest that the conformational properties of the urea-denatured states promote formation of a three-helix intermediate in which the residues that form helix III remain non-helical. Thus it appears that features of the native structure are formed early in folding linked to collapse of the unfolded state. [PUBLICATION ABSTRACT]
ISSN:0961-8368
1469-896X
DOI:10.1002/pro.2372