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Effect of dimethylsulfoxide on hydrolysis of lipase
To establish an industrially feasible reaction process, the effect of dimethylsulfoxide (DMSO) added to an aqueous solution on the hydrolysis of lipase was investigated using fluorescent substrates. Several Upases from microorganisms were improved in their hydrolysis activities against 4-methylumbel...
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| Published in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2001-09, Vol.65 (9), p.2078-2082 |
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| Main Authors: | , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c641t-70a4d76f75a291ad1ad671d9892417d677db29f9eda0f348151b2f36da1143d73 |
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| cites | cdi_FETCH-LOGICAL-c641t-70a4d76f75a291ad1ad671d9892417d677db29f9eda0f348151b2f36da1143d73 |
| container_end_page | 2082 |
| container_issue | 9 |
| container_start_page | 2078 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 65 |
| creator | Tsuzuki, W. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) Ue, A Kitamura, Y |
| description | To establish an industrially feasible reaction process, the effect of dimethylsulfoxide (DMSO) added to an aqueous solution on the hydrolysis of lipase was investigated using fluorescent substrates. Several Upases from microorganisms were improved in their hydrolysis activities against 4-methylumbelliferyl oleate by DMSO. Variation was found in the effect of DMSO depending on the species of lipase. After the high stability of the lipase from Pseudomonasfluorescens in DMSO solution was confirmed, hydrolysis by this lipase of four acy1-4-methylumbelliferones was studied kinetically at different DMSO concentrations. DMSO added to an aqueous solution increased the V submax of this lipase for a substrate with strong hydrophobicity, and decreased that value for a substrate with an opposite property. On the other hand, DMSO had a very small effect on K subm for each substrate. A fluorometric study suggested that DMSO induced a change of the chemical environment that surrounded tryptophan residues of the lipase. Such conformational change would be one of the causes of the DMSO-induced alteration of its reactive property. These results suggest that the addition of DMSO may be a novel method of 'solvent engineering' of this enzyme. |
| doi_str_mv | 10.1271/bbb.65.2078 |
| format | article |
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(National Food Research Inst., Tsukuba, Ibaraki (Japan)) ; Ue, A ; Kitamura, Y</creator><creatorcontrib>Tsuzuki, W. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) ; Ue, A ; Kitamura, Y</creatorcontrib><description>To establish an industrially feasible reaction process, the effect of dimethylsulfoxide (DMSO) added to an aqueous solution on the hydrolysis of lipase was investigated using fluorescent substrates. Several Upases from microorganisms were improved in their hydrolysis activities against 4-methylumbelliferyl oleate by DMSO. Variation was found in the effect of DMSO depending on the species of lipase. After the high stability of the lipase from Pseudomonasfluorescens in DMSO solution was confirmed, hydrolysis by this lipase of four acy1-4-methylumbelliferones was studied kinetically at different DMSO concentrations. DMSO added to an aqueous solution increased the V submax of this lipase for a substrate with strong hydrophobicity, and decreased that value for a substrate with an opposite property. On the other hand, DMSO had a very small effect on K subm for each substrate. A fluorometric study suggested that DMSO induced a change of the chemical environment that surrounded tryptophan residues of the lipase. Such conformational change would be one of the causes of the DMSO-induced alteration of its reactive property. These results suggest that the addition of DMSO may be a novel method of 'solvent engineering' of this enzyme.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.65.2078</identifier><identifier>PMID: 11676024</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Animals ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Dimethyl Sulfoxide - chemistry ; dimethylsulfoxide ; Fluorometry - methods ; Fundamental and applied biological sciences. Psychology ; HYDROLYSIS ; Kinetics ; lipase ; Lipase - chemistry ; Lipase - metabolism ; Methods. Procedures. 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(National Food Research Inst., Tsukuba, Ibaraki (Japan))</creatorcontrib><creatorcontrib>Ue, A</creatorcontrib><creatorcontrib>Kitamura, Y</creatorcontrib><title>Effect of dimethylsulfoxide on hydrolysis of lipase</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>To establish an industrially feasible reaction process, the effect of dimethylsulfoxide (DMSO) added to an aqueous solution on the hydrolysis of lipase was investigated using fluorescent substrates. Several Upases from microorganisms were improved in their hydrolysis activities against 4-methylumbelliferyl oleate by DMSO. Variation was found in the effect of DMSO depending on the species of lipase. After the high stability of the lipase from Pseudomonasfluorescens in DMSO solution was confirmed, hydrolysis by this lipase of four acy1-4-methylumbelliferones was studied kinetically at different DMSO concentrations. DMSO added to an aqueous solution increased the V submax of this lipase for a substrate with strong hydrophobicity, and decreased that value for a substrate with an opposite property. On the other hand, DMSO had a very small effect on K subm for each substrate. A fluorometric study suggested that DMSO induced a change of the chemical environment that surrounded tryptophan residues of the lipase. Such conformational change would be one of the causes of the DMSO-induced alteration of its reactive property. These results suggest that the addition of DMSO may be a novel method of 'solvent engineering' of this enzyme.</description><subject>Animals</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Dimethyl Sulfoxide - chemistry</subject><subject>dimethylsulfoxide</subject><subject>Fluorometry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HYDROLYSIS</subject><subject>Kinetics</subject><subject>lipase</subject><subject>Lipase - chemistry</subject><subject>Lipase - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Pancreas - enzymology</subject><subject>Pseudomonas fluorescens - enzymology</subject><subject>Solubility</subject><subject>Solvents - chemistry</subject><subject>TRIACYLGLYCEROL LIPASE</subject><subject>Tryptophan - analysis</subject><subject>Tryptophan - chemistry</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNpt0FFLHDEQB_BQlHpqn3yuHJT2RfaaSbLJ5lFEqyLoQ_scspuk7pHdnMkudr99s9zJgQgDCcNvhuGP0BngFRABP-u6XvFyRbCoPqEFUCYKLpk4QAssgRcVK-EIHae0xjg3SviMjgC44JiwBaLXztlmWAa3NG1nh-fJp9G78K81dhn65fNkYvBTatNMfLvRyZ6iQ6d9sl927wn6c3P9--q2eHj8dXd1-VA0nMFQCKyZEdyJUhMJ2uTiAoysJGEg8l-YmkgnrdHYUVbl02riKDcagFEj6An6sd27ieFltGlQXZsa673ubRiTEoTkTZhm-O0dXIcx9vk2BYxJxmjJSFYXW9XEkFK0Tm1i2-k4KcBqTlLlJBUv1Zxk1ue7nWPdWbO3u-gy-L4DOjXau6j7pk17R6ngUuLs-Na1vQux068heqMGPfkQ34boxxd83Q46HZT-G7O7fyIYzyUqSf8Dn9yUkw</recordid><startdate>20010901</startdate><enddate>20010901</enddate><creator>Tsuzuki, W. (National Food Research Inst., Tsukuba, Ibaraki (Japan))</creator><creator>Ue, A</creator><creator>Kitamura, Y</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010901</creationdate><title>Effect of dimethylsulfoxide on hydrolysis of lipase</title><author>Tsuzuki, W. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) ; Ue, A ; Kitamura, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-70a4d76f75a291ad1ad671d9892417d677db29f9eda0f348151b2f36da1143d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Dimethyl Sulfoxide - chemistry</topic><topic>dimethylsulfoxide</topic><topic>Fluorometry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HYDROLYSIS</topic><topic>Kinetics</topic><topic>lipase</topic><topic>Lipase - chemistry</topic><topic>Lipase - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Pancreas - enzymology</topic><topic>Pseudomonas fluorescens - enzymology</topic><topic>Solubility</topic><topic>Solvents - chemistry</topic><topic>TRIACYLGLYCEROL LIPASE</topic><topic>Tryptophan - analysis</topic><topic>Tryptophan - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuzuki, W. (National Food Research Inst., Tsukuba, Ibaraki (Japan))</creatorcontrib><creatorcontrib>Ue, A</creatorcontrib><creatorcontrib>Kitamura, Y</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuzuki, W. (National Food Research Inst., Tsukuba, Ibaraki (Japan))</au><au>Ue, A</au><au>Kitamura, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of dimethylsulfoxide on hydrolysis of lipase</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2001-09-01</date><risdate>2001</risdate><volume>65</volume><issue>9</issue><spage>2078</spage><epage>2082</epage><pages>2078-2082</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><notes>F60</notes><notes>2002002789</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>To establish an industrially feasible reaction process, the effect of dimethylsulfoxide (DMSO) added to an aqueous solution on the hydrolysis of lipase was investigated using fluorescent substrates. Several Upases from microorganisms were improved in their hydrolysis activities against 4-methylumbelliferyl oleate by DMSO. Variation was found in the effect of DMSO depending on the species of lipase. After the high stability of the lipase from Pseudomonasfluorescens in DMSO solution was confirmed, hydrolysis by this lipase of four acy1-4-methylumbelliferones was studied kinetically at different DMSO concentrations. DMSO added to an aqueous solution increased the V submax of this lipase for a substrate with strong hydrophobicity, and decreased that value for a substrate with an opposite property. On the other hand, DMSO had a very small effect on K subm for each substrate. A fluorometric study suggested that DMSO induced a change of the chemical environment that surrounded tryptophan residues of the lipase. Such conformational change would be one of the causes of the DMSO-induced alteration of its reactive property. These results suggest that the addition of DMSO may be a novel method of 'solvent engineering' of this enzyme.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>11676024</pmid><doi>10.1271/bbb.65.2078</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2001-09, Vol.65 (9), p.2078-2082 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_journals_1449443542 |
| source | Oxford University Press Journals All Titles (1996-Current); EZB Electronic Journals Library |
| subjects | Animals Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Dimethyl Sulfoxide - chemistry dimethylsulfoxide Fluorometry - methods Fundamental and applied biological sciences. Psychology HYDROLYSIS Kinetics lipase Lipase - chemistry Lipase - metabolism Methods. Procedures. Technologies Pancreas - enzymology Pseudomonas fluorescens - enzymology Solubility Solvents - chemistry TRIACYLGLYCEROL LIPASE Tryptophan - analysis Tryptophan - chemistry |
| title | Effect of dimethylsulfoxide on hydrolysis of lipase |
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