Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera)

The primary structures of two neuropeptides, Tabanus atratus adipokinetic hormone (Taa-AKH) and Tabanus atratus hypotrehalosemic hormone (Taa-HoTH), from the corpora cardiaca of horse flies (Diptera: Tabanidae) have been determined. Amino acid sequences of Taa-AKH (

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Published in:Proceedings of the National Academy of Sciences - PNAS 1989-10, Vol.86 (20), p.8161-8164
Main Authors: Jaffe, H. (ARS, USDA, Beltsville, MD), Raina, A.K, Riley, C.T, Fraser, B.A, Nachman, R.J, Vogel, V.W, Zhang, Y.S, Hayes, D.K
Format: Article
Language:English
Subjects:
Agriculture
Amino Acid Sequence
Amino acids
Animals
Corpora cardiaca
DIPTERA
Diptera - drug effects
Diptera - physiology
Dose response relationship
Enzymes
Flies
Hemolymph
Hormones
Horses
hypotrehalosemic hormone
Insect Hormones - chemical synthesis
Insect Hormones - pharmacology
Lipids
Male
Mass Spectrometry
Molecular Sequence Data
Musca autumnalis
Muscidae
Nervous System Physiological Phenomena
Neuropeptides
Oligopeptides - chemical synthesis
Oligopeptides - pharmacology
PEPTIDE
PEPTIDES
PEPTIDOS
Pyrrolidonecarboxylic Acid - analogs & derivatives
SYNTHESIS
TABANIDAE
TABANUS
Tabanus atratus
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container_issue 20
container_start_page 8161
container_title Proceedings of the National Academy of Sciences - PNAS
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creator Jaffe, H. (ARS, USDA, Beltsville, MD)
Raina, A.K
Riley, C.T
Fraser, B.A
Nachman, R.J
Vogel, V.W
Zhang, Y.S
Hayes, D.K
description The primary structures of two neuropeptides, Tabanus atratus adipokinetic hormone (Taa-AKH) and Tabanus atratus hypotrehalosemic hormone (Taa-HoTH), from the corpora cardiaca of horse flies (Diptera: Tabanidae) have been determined. Amino acid sequences of Taa-AKH (
doi_str_mv 10.1073/pnas.86.20.8161
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(ARS, USDA, Beltsville, MD)</au><au>Raina, A.K</au><au>Riley, C.T</au><au>Fraser, B.A</au><au>Nachman, R.J</au><au>Vogel, V.W</au><au>Zhang, Y.S</au><au>Hayes, D.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera)</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>86</volume><issue>20</issue><spage>8161</spage><epage>8164</epage><pages>8161-8164</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><notes>L50</notes><notes>9022147</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The primary structures of two neuropeptides, Tabanus atratus adipokinetic hormone (Taa-AKH) and Tabanus atratus hypotrehalosemic hormone (Taa-HoTH), from the corpora cardiaca of horse flies (Diptera: Tabanidae) have been determined. Amino acid sequences of Taa-AKH (&lt;Glu-Leu-Thr-Phe-Thr-Pro-Gly-Trp-NH2) and Taa-HoTH (&lt;Glu-Leu-Thr-Phe-Thr-Pro-Gly-Trp-Gly-Tyr-NH2) (where &lt;Glu = pyroglutamic acid) were determined by automated gas-phase Edman degradation of the peptides deblocked by pyroglutamate aminopeptidase and by fast atom bombardment mass spectrometry. The hormones were synthesized, and the natural and synthetic peptides exhibited identical chromatographic, spectroscopic, and biological properties. When assayed in adult face fly males, Taa-AKH and Taa-HoTH demonstrated hyperlipemic activity; in addition, Taa-HoTH also demonstrated a significant hypotrehalosemic activity.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2813385</pmid><doi>10.1073/pnas.86.20.8161</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1989-10, Vol.86 (20), p.8161-8164
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source JSTOR Archival Journals and Primary Sources Collection; PubMed Central
subjects Agriculture
Amino Acid Sequence
Amino acids
Animals
Corpora cardiaca
DIPTERA
Diptera - drug effects
Diptera - physiology
Dose response relationship
Enzymes
Flies
Hemolymph
Hormones
Horses
hypotrehalosemic hormone
Insect Hormones - chemical synthesis
Insect Hormones - pharmacology
Lipids
Male
Mass Spectrometry
Molecular Sequence Data
Musca autumnalis
Muscidae
Nervous System Physiological Phenomena
Neuropeptides
Oligopeptides - chemical synthesis
Oligopeptides - pharmacology
PEPTIDE
PEPTIDES
PEPTIDOS
Pyrrolidonecarboxylic Acid - analogs & derivatives
SYNTHESIS
TABANIDAE
TABANUS
Tabanus atratus
title Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera)
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