Study of the colonic epithelial-mesenchymal dialogue through establishment of two activated or not mesenchymal cell lines: Activated and resting ones differentially modulate colonocytes in co-culture

Continuous and rapid renewal of the colonic epithelium is crucial to resist the plethora of luminal deleterious agents. Subepithelial fibroblasts contribute to this turnover by regulating epithelial proliferation and differentiation. However, when intestinal homeostasis is disturbed, fibroblasts can...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2022-08, Vol.17 (8), p.e0273858-e0273858
Main Authors: Plaisancié, Pascale, Buisson, Charline, Fouché, Edwin, Martin, Pierre, Noirot, Céline, Maslo, Claire, Dupuy, Jacques, Guéraud, Françoise, Pierre, Fabrice
Format: Article
Language:English
Subjects:
Analysis
Antigens
Apoptosis
Bioinformatics
Biology and Life Sciences
Biomarkers
Cell culture
Cell lines
Cloning
Collagen
Colorectal cancer
Cytokeratin
Cytokines
E-cadherin
Epithelium
Extracellular matrix
Fibroblasts
Genomes
Health aspects
Homeostasis
Immunofluorescence
Inflammation
Inflammatory bowel disease
Intestine
Life Sciences
Medicine and Health Sciences
Mesenchyme
Mutation
Paracrine signalling
Phenotypes
Proteins
Research and Analysis Methods
Stem cells
Vimentin
Wnt protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53
cites cdi_FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53
container_end_page e0273858
container_issue 8
container_start_page e0273858
container_title PloS one
container_volume 17
creator Plaisancié, Pascale
Buisson, Charline
Fouché, Edwin
Martin, Pierre
Noirot, Céline
Maslo, Claire
Dupuy, Jacques
Guéraud, Françoise
Pierre, Fabrice
description Continuous and rapid renewal of the colonic epithelium is crucial to resist the plethora of luminal deleterious agents. Subepithelial fibroblasts contribute to this turnover by regulating epithelial proliferation and differentiation. However, when intestinal homeostasis is disturbed, fibroblasts can acquire an activated phenotype and play a major role in the progression of intestinal pathologies. To evaluate the involvement of fibroblasts in the regulation of colonocytes under homeostatic or pathological conditions, we established resting and activated conditionally immortalized fibroblast cell lines (nF and mF) from mouse colonic mucosa. We then studied the epithelial-mesenchymal interactions between activated or resting fibroblasts and the normal mouse colonocytes (Co) using a co-culture model. Both fibroblastic cell lines were characterized by RT-qPCR, western blot and immunofluorescence assay. Our results showed that nF and mF cells were positive for fibroblastic markers such as vimentin and collagen 1, and negative for cytokeratin 18 and E-cadherin, attesting to their fibroblastic type. They also expressed proteins characteristic of the epithelial stem cell niche such as Grem1, CD90 or Wnt5a. Only rare nF fibroblasts were positive for [alpha]-SMA, whereas all mF fibroblasts strongly expressed this marker, supporting that mF cells were activated fibroblasts/myofibroblasts. In coculture, nF fibroblasts and Co cells strongly interacted via paracrine exchanges resulting in BMP4 production in nF fibroblasts, activation of BMP signaling in Co colonocytes, and decreased growth of colonocytes. Activated-type mF fibroblasts did not exert the same effects on Co cells, allowing colonocytes free to proliferate. In conclusion, these two colonic fibroblast lines, associated with Co cells in coculture, should allow to better understand the role of mesenchymal cells in the preservation of homeostasis and the development of intestinal pathologies.
doi_str_mv 10.1371/journal.pone.0273858
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2708225939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A715527634</galeid><doaj_id>oai_doaj_org_article_a018ff879f6f4e07934893b16cddb216</doaj_id><sourcerecordid>A715527634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53</originalsourceid><addsrcrecordid>eNqNk91qFDEUxwdRbK2-gWBAEHuxazKZr3ghLEVtoVCw6m3IZJKZlEyyTTLVfUJfy7PdsXRLL2QuMjnzO__zNSfLXhO8JLQmH678FJywy7V3aonzmjZl8yQ7JIzmiyrH9Om994PsRYxXGJe0qarn2QGtcIFZUx5mfy7T1G2Q1ygNCklvvTMSqbWBqzXCLkYVlZPDZhQWdWDw_aSADX7qB6RiEq01cRiVS7civzwSMpkbkVSHfEDOJ3RfQiprkTVOxY9odQcK16EAYsb1CMqJEElrFUAUItoNGn03WSB3CXq5ScAYB9eFnGyagnqZPdPCRvVqPo-yH18-fz85XZxffD07WZ0vZI1pWjRSaVHmsq0L2eYsV6xUTFeENBUjDakFa7GUrFaSsrLQFMsKiw7TuuqIKLqSHmVvdrpr6yOfZxB5XuMmz0tGGRBnO6Lz4oqvgxlF2HAvDL81-NBzEZKRVnGBSaN1U0MGulC4ZrRoGG1JJbuuzUkFWp_maFM7qk5CQ4Kwe6L7X5wZeO9vOCvyqqm3Asc7geGB2-nqnG9tUFpdEVreEGDfz8GCv55gGnw0cTsv4ZSf5hpLRmgO6NsH6OOdmKleQLHGaQ85yq0oX9WkLHNIsABq-QgFT6dGI-Fv0Absew7Hew7AJPU79WKKkZ9dfvt_9uLnPvvuHjsoYdMQvZ2S8S7ug8UOlMHHGJS-6yzBfLub_7rBt7vJ592kfwFQUS7X</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2708225939</pqid></control><display><type>article</type><title>Study of the colonic epithelial-mesenchymal dialogue through establishment of two activated or not mesenchymal cell lines: Activated and resting ones differentially modulate colonocytes in co-culture</title><source>PubMed (Medline)</source><source>Publicly Available Content Database</source><creator>Plaisancié, Pascale ; Buisson, Charline ; Fouché, Edwin ; Martin, Pierre ; Noirot, Céline ; Maslo, Claire ; Dupuy, Jacques ; Guéraud, Françoise ; Pierre, Fabrice</creator><contributor>Sun, Jun</contributor><creatorcontrib>Plaisancié, Pascale ; Buisson, Charline ; Fouché, Edwin ; Martin, Pierre ; Noirot, Céline ; Maslo, Claire ; Dupuy, Jacques ; Guéraud, Françoise ; Pierre, Fabrice ; Sun, Jun</creatorcontrib><description>Continuous and rapid renewal of the colonic epithelium is crucial to resist the plethora of luminal deleterious agents. Subepithelial fibroblasts contribute to this turnover by regulating epithelial proliferation and differentiation. However, when intestinal homeostasis is disturbed, fibroblasts can acquire an activated phenotype and play a major role in the progression of intestinal pathologies. To evaluate the involvement of fibroblasts in the regulation of colonocytes under homeostatic or pathological conditions, we established resting and activated conditionally immortalized fibroblast cell lines (nF and mF) from mouse colonic mucosa. We then studied the epithelial-mesenchymal interactions between activated or resting fibroblasts and the normal mouse colonocytes (Co) using a co-culture model. Both fibroblastic cell lines were characterized by RT-qPCR, western blot and immunofluorescence assay. Our results showed that nF and mF cells were positive for fibroblastic markers such as vimentin and collagen 1, and negative for cytokeratin 18 and E-cadherin, attesting to their fibroblastic type. They also expressed proteins characteristic of the epithelial stem cell niche such as Grem1, CD90 or Wnt5a. Only rare nF fibroblasts were positive for [alpha]-SMA, whereas all mF fibroblasts strongly expressed this marker, supporting that mF cells were activated fibroblasts/myofibroblasts. In coculture, nF fibroblasts and Co cells strongly interacted via paracrine exchanges resulting in BMP4 production in nF fibroblasts, activation of BMP signaling in Co colonocytes, and decreased growth of colonocytes. Activated-type mF fibroblasts did not exert the same effects on Co cells, allowing colonocytes free to proliferate. In conclusion, these two colonic fibroblast lines, associated with Co cells in coculture, should allow to better understand the role of mesenchymal cells in the preservation of homeostasis and the development of intestinal pathologies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0273858</identifier><identifier>PMID: 36040985</identifier><language>eng</language><publisher>San Francisco: Public Library of Science</publisher><subject>Analysis ; Antigens ; Apoptosis ; Bioinformatics ; Biology and Life Sciences ; Biomarkers ; Cell culture ; Cell lines ; Cloning ; Collagen ; Colorectal cancer ; Cytokeratin ; Cytokines ; E-cadherin ; Epithelium ; Extracellular matrix ; Fibroblasts ; Genomes ; Health aspects ; Homeostasis ; Immunofluorescence ; Inflammation ; Inflammatory bowel disease ; Intestine ; Life Sciences ; Medicine and Health Sciences ; Mesenchyme ; Mutation ; Paracrine signalling ; Phenotypes ; Proteins ; Research and Analysis Methods ; Stem cells ; Vimentin ; Wnt protein</subject><ispartof>PloS one, 2022-08, Vol.17 (8), p.e0273858-e0273858</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Plaisancié et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><rights>2022 Plaisancié et al 2022 Plaisancié et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53</citedby><cites>FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53</cites><orcidid>0000-0003-1289-2870 ; 0000-0001-5455-1244 ; 0000-0002-3670-6684 ; 0000-0001-6106-8146</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2708225939/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2708225939?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,37048,44625,53827,53829,75483</link.rule.ids><backlink>$$Uhttps://hal.inrae.fr/hal-03776135$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Sun, Jun</contributor><creatorcontrib>Plaisancié, Pascale</creatorcontrib><creatorcontrib>Buisson, Charline</creatorcontrib><creatorcontrib>Fouché, Edwin</creatorcontrib><creatorcontrib>Martin, Pierre</creatorcontrib><creatorcontrib>Noirot, Céline</creatorcontrib><creatorcontrib>Maslo, Claire</creatorcontrib><creatorcontrib>Dupuy, Jacques</creatorcontrib><creatorcontrib>Guéraud, Françoise</creatorcontrib><creatorcontrib>Pierre, Fabrice</creatorcontrib><title>Study of the colonic epithelial-mesenchymal dialogue through establishment of two activated or not mesenchymal cell lines: Activated and resting ones differentially modulate colonocytes in co-culture</title><title>PloS one</title><description>Continuous and rapid renewal of the colonic epithelium is crucial to resist the plethora of luminal deleterious agents. Subepithelial fibroblasts contribute to this turnover by regulating epithelial proliferation and differentiation. However, when intestinal homeostasis is disturbed, fibroblasts can acquire an activated phenotype and play a major role in the progression of intestinal pathologies. To evaluate the involvement of fibroblasts in the regulation of colonocytes under homeostatic or pathological conditions, we established resting and activated conditionally immortalized fibroblast cell lines (nF and mF) from mouse colonic mucosa. We then studied the epithelial-mesenchymal interactions between activated or resting fibroblasts and the normal mouse colonocytes (Co) using a co-culture model. Both fibroblastic cell lines were characterized by RT-qPCR, western blot and immunofluorescence assay. Our results showed that nF and mF cells were positive for fibroblastic markers such as vimentin and collagen 1, and negative for cytokeratin 18 and E-cadherin, attesting to their fibroblastic type. They also expressed proteins characteristic of the epithelial stem cell niche such as Grem1, CD90 or Wnt5a. Only rare nF fibroblasts were positive for [alpha]-SMA, whereas all mF fibroblasts strongly expressed this marker, supporting that mF cells were activated fibroblasts/myofibroblasts. In coculture, nF fibroblasts and Co cells strongly interacted via paracrine exchanges resulting in BMP4 production in nF fibroblasts, activation of BMP signaling in Co colonocytes, and decreased growth of colonocytes. Activated-type mF fibroblasts did not exert the same effects on Co cells, allowing colonocytes free to proliferate. In conclusion, these two colonic fibroblast lines, associated with Co cells in coculture, should allow to better understand the role of mesenchymal cells in the preservation of homeostasis and the development of intestinal pathologies.</description><subject>Analysis</subject><subject>Antigens</subject><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Cell culture</subject><subject>Cell lines</subject><subject>Cloning</subject><subject>Collagen</subject><subject>Colorectal cancer</subject><subject>Cytokeratin</subject><subject>Cytokines</subject><subject>E-cadherin</subject><subject>Epithelium</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Homeostasis</subject><subject>Immunofluorescence</subject><subject>Inflammation</subject><subject>Inflammatory bowel disease</subject><subject>Intestine</subject><subject>Life Sciences</subject><subject>Medicine and Health Sciences</subject><subject>Mesenchyme</subject><subject>Mutation</subject><subject>Paracrine signalling</subject><subject>Phenotypes</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Stem cells</subject><subject>Vimentin</subject><subject>Wnt protein</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk91qFDEUxwdRbK2-gWBAEHuxazKZr3ghLEVtoVCw6m3IZJKZlEyyTTLVfUJfy7PdsXRLL2QuMjnzO__zNSfLXhO8JLQmH678FJywy7V3aonzmjZl8yQ7JIzmiyrH9Om994PsRYxXGJe0qarn2QGtcIFZUx5mfy7T1G2Q1ygNCklvvTMSqbWBqzXCLkYVlZPDZhQWdWDw_aSADX7qB6RiEq01cRiVS7civzwSMpkbkVSHfEDOJ3RfQiprkTVOxY9odQcK16EAYsb1CMqJEElrFUAUItoNGn03WSB3CXq5ScAYB9eFnGyagnqZPdPCRvVqPo-yH18-fz85XZxffD07WZ0vZI1pWjRSaVHmsq0L2eYsV6xUTFeENBUjDakFa7GUrFaSsrLQFMsKiw7TuuqIKLqSHmVvdrpr6yOfZxB5XuMmz0tGGRBnO6Lz4oqvgxlF2HAvDL81-NBzEZKRVnGBSaN1U0MGulC4ZrRoGG1JJbuuzUkFWp_maFM7qk5CQ4Kwe6L7X5wZeO9vOCvyqqm3Asc7geGB2-nqnG9tUFpdEVreEGDfz8GCv55gGnw0cTsv4ZSf5hpLRmgO6NsH6OOdmKleQLHGaQ85yq0oX9WkLHNIsABq-QgFT6dGI-Fv0Absew7Hew7AJPU79WKKkZ9dfvt_9uLnPvvuHjsoYdMQvZ2S8S7ug8UOlMHHGJS-6yzBfLub_7rBt7vJ592kfwFQUS7X</recordid><startdate>20220830</startdate><enddate>20220830</enddate><creator>Plaisancié, Pascale</creator><creator>Buisson, Charline</creator><creator>Fouché, Edwin</creator><creator>Martin, Pierre</creator><creator>Noirot, Céline</creator><creator>Maslo, Claire</creator><creator>Dupuy, Jacques</creator><creator>Guéraud, Françoise</creator><creator>Pierre, Fabrice</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-1289-2870</orcidid><orcidid>https://orcid.org/0000-0001-5455-1244</orcidid><orcidid>https://orcid.org/0000-0002-3670-6684</orcidid><orcidid>https://orcid.org/0000-0001-6106-8146</orcidid></search><sort><creationdate>20220830</creationdate><title>Study of the colonic epithelial-mesenchymal dialogue through establishment of two activated or not mesenchymal cell lines: Activated and resting ones differentially modulate colonocytes in co-culture</title><author>Plaisancié, Pascale ; Buisson, Charline ; Fouché, Edwin ; Martin, Pierre ; Noirot, Céline ; Maslo, Claire ; Dupuy, Jacques ; Guéraud, Françoise ; Pierre, Fabrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Antigens</topic><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Cell culture</topic><topic>Cell lines</topic><topic>Cloning</topic><topic>Collagen</topic><topic>Colorectal cancer</topic><topic>Cytokeratin</topic><topic>Cytokines</topic><topic>E-cadherin</topic><topic>Epithelium</topic><topic>Extracellular matrix</topic><topic>Fibroblasts</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>Homeostasis</topic><topic>Immunofluorescence</topic><topic>Inflammation</topic><topic>Inflammatory bowel disease</topic><topic>Intestine</topic><topic>Life Sciences</topic><topic>Medicine and Health Sciences</topic><topic>Mesenchyme</topic><topic>Mutation</topic><topic>Paracrine signalling</topic><topic>Phenotypes</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Stem cells</topic><topic>Vimentin</topic><topic>Wnt protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Plaisancié, Pascale</creatorcontrib><creatorcontrib>Buisson, Charline</creatorcontrib><creatorcontrib>Fouché, Edwin</creatorcontrib><creatorcontrib>Martin, Pierre</creatorcontrib><creatorcontrib>Noirot, Céline</creatorcontrib><creatorcontrib>Maslo, Claire</creatorcontrib><creatorcontrib>Dupuy, Jacques</creatorcontrib><creatorcontrib>Guéraud, Françoise</creatorcontrib><creatorcontrib>Pierre, Fabrice</creatorcontrib><collection>CrossRef</collection><collection>Opposing Viewpoints Resource Center</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Database‎ (1962 - current)</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Plaisancié, Pascale</au><au>Buisson, Charline</au><au>Fouché, Edwin</au><au>Martin, Pierre</au><au>Noirot, Céline</au><au>Maslo, Claire</au><au>Dupuy, Jacques</au><au>Guéraud, Françoise</au><au>Pierre, Fabrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of the colonic epithelial-mesenchymal dialogue through establishment of two activated or not mesenchymal cell lines: Activated and resting ones differentially modulate colonocytes in co-culture</atitle><jtitle>PloS one</jtitle><date>2022-08-30</date><risdate>2022</risdate><volume>17</volume><issue>8</issue><spage>e0273858</spage><epage>e0273858</epage><pages>e0273858-e0273858</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Competing Interests: The authors have declared that no competing interests exist</notes><abstract>Continuous and rapid renewal of the colonic epithelium is crucial to resist the plethora of luminal deleterious agents. Subepithelial fibroblasts contribute to this turnover by regulating epithelial proliferation and differentiation. However, when intestinal homeostasis is disturbed, fibroblasts can acquire an activated phenotype and play a major role in the progression of intestinal pathologies. To evaluate the involvement of fibroblasts in the regulation of colonocytes under homeostatic or pathological conditions, we established resting and activated conditionally immortalized fibroblast cell lines (nF and mF) from mouse colonic mucosa. We then studied the epithelial-mesenchymal interactions between activated or resting fibroblasts and the normal mouse colonocytes (Co) using a co-culture model. Both fibroblastic cell lines were characterized by RT-qPCR, western blot and immunofluorescence assay. Our results showed that nF and mF cells were positive for fibroblastic markers such as vimentin and collagen 1, and negative for cytokeratin 18 and E-cadherin, attesting to their fibroblastic type. They also expressed proteins characteristic of the epithelial stem cell niche such as Grem1, CD90 or Wnt5a. Only rare nF fibroblasts were positive for [alpha]-SMA, whereas all mF fibroblasts strongly expressed this marker, supporting that mF cells were activated fibroblasts/myofibroblasts. In coculture, nF fibroblasts and Co cells strongly interacted via paracrine exchanges resulting in BMP4 production in nF fibroblasts, activation of BMP signaling in Co colonocytes, and decreased growth of colonocytes. Activated-type mF fibroblasts did not exert the same effects on Co cells, allowing colonocytes free to proliferate. In conclusion, these two colonic fibroblast lines, associated with Co cells in coculture, should allow to better understand the role of mesenchymal cells in the preservation of homeostasis and the development of intestinal pathologies.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>36040985</pmid><doi>10.1371/journal.pone.0273858</doi><tpages>e0273858</tpages><orcidid>https://orcid.org/0000-0003-1289-2870</orcidid><orcidid>https://orcid.org/0000-0001-5455-1244</orcidid><orcidid>https://orcid.org/0000-0002-3670-6684</orcidid><orcidid>https://orcid.org/0000-0001-6106-8146</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2022-08, Vol.17 (8), p.e0273858-e0273858
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_2708225939
source PubMed (Medline); Publicly Available Content Database
subjects Analysis
Antigens
Apoptosis
Bioinformatics
Biology and Life Sciences
Biomarkers
Cell culture
Cell lines
Cloning
Collagen
Colorectal cancer
Cytokeratin
Cytokines
E-cadherin
Epithelium
Extracellular matrix
Fibroblasts
Genomes
Health aspects
Homeostasis
Immunofluorescence
Inflammation
Inflammatory bowel disease
Intestine
Life Sciences
Medicine and Health Sciences
Mesenchyme
Mutation
Paracrine signalling
Phenotypes
Proteins
Research and Analysis Methods
Stem cells
Vimentin
Wnt protein
title Study of the colonic epithelial-mesenchymal dialogue through establishment of two activated or not mesenchymal cell lines: Activated and resting ones differentially modulate colonocytes in co-culture
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T18%3A30%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Study%20of%20the%20colonic%20epithelial-mesenchymal%20dialogue%20through%20establishment%20of%20two%20activated%20or%20not%20mesenchymal%20cell%20lines:%20Activated%20and%20resting%20ones%20differentially%20modulate%20colonocytes%20in%20co-culture&rft.jtitle=PloS%20one&rft.au=Plaisanci%C3%A9,%20Pascale&rft.date=2022-08-30&rft.volume=17&rft.issue=8&rft.spage=e0273858&rft.epage=e0273858&rft.pages=e0273858-e0273858&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0273858&rft_dat=%3Cgale_plos_%3EA715527634%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c703t-8cefa52cb74cb292e95e9f6118691817a9b0cc97ec3954f30c60ad0376d1a4d53%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2708225939&rft_id=info:pmid/36040985&rft_galeid=A715527634&rfr_iscdi=true