Similar hemostatic responses to hypovolemia induced by hemorrhage and lower body negative pressure reveal a hyperfibrinolytic subset of non-human primates

To study central hypovolemia in humans, lower body negative pressure (LBNP) is a recognized alternative to blood removal (HEM). While LBNP mimics the cardiovascular responses of HEM in baboons, similarities in hemostatic responses to LBNP and HEM remain unknown in this species. Thirteen anesthetized...

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Bibliographic Details
Published in:PloS one 2020-06, Vol.15 (6), p.e0234844-e0234844
Main Authors: Zaar, Morten, Herzig, Maryanne C, Fedyk, Chriselda G, Montgomery, Robbie K, Prat, Nicolas, Parida, Bijaya K, Hinojosa-Laborde, Carmen, Muniz, Gary W, Shade, Robert E, Bauer, Cassondra, Delacruz, Wilfred, Bynum, James A, Convertino, Victor A, Cap, Andrew P, Pidcoke, Heather F
Format: Article
Language:English
Subjects:
Activation
Animal welfare
Baboons
Biology and life sciences
Biomedical research
Blood & organ donations
Blood coagulation factors
Body fluid disorders
Cardiovascular system
Coagulation
Complications and side effects
Development and progression
Fibrin
Fibrinolysis
Flow cytometry
Health aspects
Hematology
Hemorrhage
Hemostasis
Hypovolemia
Inhibitors
Laboratory animals
Lower body negative pressure
Lysis
Medicine and Health Sciences
Monkeys & apes
Physiological aspects
Physiology
Plasma
Plasmin
Plasminogen activator inhibitors
Platelets
Primates
Protein C
Proteins
t-Plasminogen activator
Thrombin
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Summary:To study central hypovolemia in humans, lower body negative pressure (LBNP) is a recognized alternative to blood removal (HEM). While LBNP mimics the cardiovascular responses of HEM in baboons, similarities in hemostatic responses to LBNP and HEM remain unknown in this species. Thirteen anesthetized baboons were exposed to progressive hypovolemia by HEM and, four weeks later, by LBNP. Hemostatic activity was evaluated by plasma markers, thromboelastography (TEG), flow cytometry, and platelet aggregometry at baseline (BL), during and after hypovolemia. BL values were indistinguishable for most parameters although platelet count, maximal clot strength (MA), protein C, thrombin anti-thrombin complex (TAT), thrombin activatable fibrinolysis inhibitor (TAFI) activity significantly differed between HEM and LBNP. Central hypovolemia induced by either method activated coagulation; TEG R-time decreased and MA increased during and after hypovolemia compared to BL. Platelets displayed activation by flow cytometry; platelet count and functional aggregometry were unchanged. TAFI activity and protein, Factors V and VIII, vWF, Proteins C and S all demonstrated hemodilution during HEM and hemoconcentration during LBNP, whereas tissue plasminogen activator (tPA), plasmin/anti-plasmin complex, and plasminogen activator inhibitor-1 did not. Fibrinolysis (TEG LY30) was unchanged by either method; however, at BL, fibrinolysis varied greatly. Post-hoc analysis separated baboons into low-lysis (LY30 2%) whose fibrinolytic state matched at both HEM and LBNP BL. In high-lysis, BL tPA and LY30 correlated strongly (r = 0.95; P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0234844