Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses

Yersinia pseudotuberculosis is an important foodborne pathogen threatening modern food safety due to its ability to survive and grow at low temperatures. DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total...

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Published in:PloS one 2019-07, Vol.14 (7), p.e0219422-e0219422
Main Authors: Jiang, Xiaojie, Keto-Timonen, Riikka, Skurnik, Mikael, Korkeala, Hannu
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Cold
Cold shock
Cold Temperature
Cold tolerance
DEAD-box RNA Helicases - genetics
DNA helicase
E coli
Environmental health
Ethanol
Ethanol - metabolism
Food
Food production
Food safety
Foodborne pathogens
Genes
Genetic aspects
Helicases
Hydrogen-Ion Concentration
Hygiene
Identification and classification
Listeria
Low temperature
Medicine and Health Sciences
Messenger RNA
Microbial metabolism
Mutation
Osmotic Pressure
Oxidative Stress
pH effects
Physical Sciences
Physiological aspects
Plasmids
Polymerase chain reaction
Proteins
Pseudotuberculosis
Reverse transcription
Ribonucleic acid
RNA
RNA helicase
Shock
Temperature effects
Transcription (Genetics)
Veterinary medicine
Yersinia
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis - genetics
Yersinia pseudotuberculosis - metabolism
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creator Jiang, Xiaojie
Keto-Timonen, Riikka
Skurnik, Mikael
Korkeala, Hannu
description Yersinia pseudotuberculosis is an important foodborne pathogen threatening modern food safety due to its ability to survive and grow at low temperatures. DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total of five DEAD-box RNA helicase genes (rhlB, csdA, rhlE, dbpA, srmB) have been identified in Y. pseudotuberculosis IP32953. However, their role in various stress conditions used in food production is unclear. We studied the involvement of the DEAD-box RNA helicase-encoding genes in the cold tolerance of Y. pseudotuberculosis IP32953 using quantitative real-time reverse transcription (RT-qPCR) and mutational analysis. Quantitative RT-PCR revealed that mRNA transcriptional levels of csdA, rhlE, dbpA and srmB were significantly higher after cold shock at 3°C compared to non-shocked culture at 28°C, suggesting the involvement of these four genes in cold shock response at the transcriptional level. The deletion of csdA ceased growth, while the deletion of dbpA or srmB significantly impaired growth at 3°C, suggesting the requirement of these three genes in Y. pseudotuberculosis at low temperatures. Growth of each DEAD-box RNA helicase mutant was also investigated under pH, osmotic, ethanol and oxidative stress conditions. The five helicase-encoding genes did not play major roles in the growth of Y. pseudotuberculosis IP32953 under pH, osmotic, ethanol or oxidative stress.
doi_str_mv 10.1371/journal.pone.0219422
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DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total of five DEAD-box RNA helicase genes (rhlB, csdA, rhlE, dbpA, srmB) have been identified in Y. pseudotuberculosis IP32953. However, their role in various stress conditions used in food production is unclear. We studied the involvement of the DEAD-box RNA helicase-encoding genes in the cold tolerance of Y. pseudotuberculosis IP32953 using quantitative real-time reverse transcription (RT-qPCR) and mutational analysis. Quantitative RT-PCR revealed that mRNA transcriptional levels of csdA, rhlE, dbpA and srmB were significantly higher after cold shock at 3°C compared to non-shocked culture at 28°C, suggesting the involvement of these four genes in cold shock response at the transcriptional level. 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DEAD-box RNA helicase CsdA has been shown to play an important role in the low-temperature growth of psychrotrophic Y. pseudotuberculosis. A total of five DEAD-box RNA helicase genes (rhlB, csdA, rhlE, dbpA, srmB) have been identified in Y. pseudotuberculosis IP32953. However, their role in various stress conditions used in food production is unclear. We studied the involvement of the DEAD-box RNA helicase-encoding genes in the cold tolerance of Y. pseudotuberculosis IP32953 using quantitative real-time reverse transcription (RT-qPCR) and mutational analysis. Quantitative RT-PCR revealed that mRNA transcriptional levels of csdA, rhlE, dbpA and srmB were significantly higher after cold shock at 3°C compared to non-shocked culture at 28°C, suggesting the involvement of these four genes in cold shock response at the transcriptional level. The deletion of csdA ceased growth, while the deletion of dbpA or srmB significantly impaired growth at 3°C, suggesting the requirement of these three genes in Y. pseudotuberculosis at low temperatures. Growth of each DEAD-box RNA helicase mutant was also investigated under pH, osmotic, ethanol and oxidative stress conditions. The five helicase-encoding genes did not play major roles in the growth of Y. pseudotuberculosis IP32953 under pH, osmotic, ethanol or oxidative stress.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31287844</pmid><doi>10.1371/journal.pone.0219422</doi><tpages>e0219422</tpages><orcidid>https://orcid.org/0000-0002-4823-9139</orcidid><orcidid>https://orcid.org/0000-0001-8791-9260</orcidid><oa>free_for_read</oa></addata></record>
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1932-6203
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source Publicly Available Content Database; PubMed Central
subjects Biology and Life Sciences
Cold
Cold shock
Cold Temperature
Cold tolerance
DEAD-box RNA Helicases - genetics
DNA helicase
E coli
Environmental health
Ethanol
Ethanol - metabolism
Food
Food production
Food safety
Foodborne pathogens
Genes
Genetic aspects
Helicases
Hydrogen-Ion Concentration
Hygiene
Identification and classification
Listeria
Low temperature
Medicine and Health Sciences
Messenger RNA
Microbial metabolism
Mutation
Osmotic Pressure
Oxidative Stress
pH effects
Physical Sciences
Physiological aspects
Plasmids
Polymerase chain reaction
Proteins
Pseudotuberculosis
Reverse transcription
Ribonucleic acid
RNA
RNA helicase
Shock
Temperature effects
Transcription (Genetics)
Veterinary medicine
Yersinia
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis - genetics
Yersinia pseudotuberculosis - metabolism
title Role of DEAD-box RNA helicase genes in the growth of Yersinia pseudotuberculosis IP32953 under cold, pH, osmotic, ethanol and oxidative stresses
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