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Scaffold-based lung tumor culture on porous PLGA microparticle substrates
Scaffold-based cancer cell culture techniques have been gaining prominence especially in the last two decades. These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and unc...
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| Published in: | PloS one 2019-05, Vol.14 (5), p.e0217640-e0217640 |
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| creator | Kuriakose, Aneetta E Hu, Wenjing Nguyen, Kytai T Menon, Jyothi U |
| description | Scaffold-based cancer cell culture techniques have been gaining prominence especially in the last two decades. These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and uncontrollable size of cell aggregates. Porous scaffolds can provide a convenient support for cell attachment, proliferation and migration, and also allows diffusion of oxygen, nutrients and waste. In this paper, a comparative study was done on porous poly (lactic-co-glycolic acid) (PLGA) microparticles prepared using three porogens-gelatin, sodium bicarbonate (SBC) or novel poly N-isopropylacrylamide [PNIPAAm] particles, as substrates for lung cancer cell culture. These fibronectin-coated, stable particles (19-42 μm) supported A549 cell attachment at an optimal cell seeding density of 250,000 cells/ mg of particles. PLGA-SBC porous particles had comparatively larger, more interconnected pores, and favored greater cell proliferation up to 9 days than their counterparts. This indicates that pore diameters and interconnectivity have direct implications on scaffold-based cell culture compared to substrates with minimally interconnected pores (PLGA-gelatin) or pores of uniform sizes (PLGA-PMPs). Therefore, PLGA-SBC-based tumor models were chosen for preliminary drug screening studies. The greater drug resistance observed in the lung cancer cells grown on porous particles compared to conventional cell monolayers agrees with previous literature, and indicates that the PLGA-SBC porous microparticle substrates are promising for in vitro tumor or tissue development. |
| doi_str_mv | 10.1371/journal.pone.0217640 |
| format | article |
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These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and uncontrollable size of cell aggregates. Porous scaffolds can provide a convenient support for cell attachment, proliferation and migration, and also allows diffusion of oxygen, nutrients and waste. In this paper, a comparative study was done on porous poly (lactic-co-glycolic acid) (PLGA) microparticles prepared using three porogens-gelatin, sodium bicarbonate (SBC) or novel poly N-isopropylacrylamide [PNIPAAm] particles, as substrates for lung cancer cell culture. These fibronectin-coated, stable particles (19-42 μm) supported A549 cell attachment at an optimal cell seeding density of 250,000 cells/ mg of particles. PLGA-SBC porous particles had comparatively larger, more interconnected pores, and favored greater cell proliferation up to 9 days than their counterparts. This indicates that pore diameters and interconnectivity have direct implications on scaffold-based cell culture compared to substrates with minimally interconnected pores (PLGA-gelatin) or pores of uniform sizes (PLGA-PMPs). Therefore, PLGA-SBC-based tumor models were chosen for preliminary drug screening studies. The greater drug resistance observed in the lung cancer cells grown on porous particles compared to conventional cell monolayers agrees with previous literature, and indicates that the PLGA-SBC porous microparticle substrates are promising for in vitro tumor or tissue development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0217640</identifier><identifier>PMID: 31150477</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bioengineering ; Biology and Life Sciences ; Biomedical engineering ; Biotechnology ; Cell adhesion ; Cell Adhesion - drug effects ; Cell Count ; Cell culture ; Cell Culture Techniques ; Cell death ; Cell migration ; Cell proliferation ; Cell Proliferation - drug effects ; Cell size ; Cell Survival - drug effects ; Cell-Derived Microparticles - drug effects ; Comparative studies ; Culture techniques ; Diffusion ; Drug Evaluation, Preclinical - methods ; Drug resistance ; Drug Resistance, Neoplasm - drug effects ; Drug screening ; Extracellular matrix ; Fibronectin ; Gelatin ; Gelatin - chemistry ; Gelatin - pharmacology ; Glycolic acid ; Humans ; Isopropylacrylamide ; Lung cancer ; Lung diseases ; Lung Neoplasms - drug therapy ; Lung Neoplasms - pathology ; Medicine and Health Sciences ; Metabolism ; Methods ; Microparticles ; Nutrients ; Oxygen ; Polylactic acid ; Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology ; Polylactide-co-glycolide ; Pores ; Porosity ; Research and Analysis Methods ; Scaffolds ; Sodium ; Sodium bicarbonate ; Spheroids ; Substrates ; Tissue Engineering ; Tissue Scaffolds - chemistry ; Tumors</subject><ispartof>PloS one, 2019-05, Vol.14 (5), p.e0217640-e0217640</ispartof><rights>2019 Kuriakose et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 Kuriakose et al 2019 Kuriakose et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-cfc010b6c010621d9512e81dae0ea11dbe0fb979ca415b0647cc216398d9ffb33</citedby><cites>FETCH-LOGICAL-c526t-cfc010b6c010621d9512e81dae0ea11dbe0fb979ca415b0647cc216398d9ffb33</cites><orcidid>0000-0002-0762-3513</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2233259931/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2233259931?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,37048,44625,53827,53829,75483</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31150477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Cordes, Nils</contributor><creatorcontrib>Kuriakose, Aneetta E</creatorcontrib><creatorcontrib>Hu, Wenjing</creatorcontrib><creatorcontrib>Nguyen, Kytai T</creatorcontrib><creatorcontrib>Menon, Jyothi U</creatorcontrib><title>Scaffold-based lung tumor culture on porous PLGA microparticle substrates</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Scaffold-based cancer cell culture techniques have been gaining prominence especially in the last two decades. These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and uncontrollable size of cell aggregates. Porous scaffolds can provide a convenient support for cell attachment, proliferation and migration, and also allows diffusion of oxygen, nutrients and waste. In this paper, a comparative study was done on porous poly (lactic-co-glycolic acid) (PLGA) microparticles prepared using three porogens-gelatin, sodium bicarbonate (SBC) or novel poly N-isopropylacrylamide [PNIPAAm] particles, as substrates for lung cancer cell culture. These fibronectin-coated, stable particles (19-42 μm) supported A549 cell attachment at an optimal cell seeding density of 250,000 cells/ mg of particles. PLGA-SBC porous particles had comparatively larger, more interconnected pores, and favored greater cell proliferation up to 9 days than their counterparts. This indicates that pore diameters and interconnectivity have direct implications on scaffold-based cell culture compared to substrates with minimally interconnected pores (PLGA-gelatin) or pores of uniform sizes (PLGA-PMPs). Therefore, PLGA-SBC-based tumor models were chosen for preliminary drug screening studies. The greater drug resistance observed in the lung cancer cells grown on porous particles compared to conventional cell monolayers agrees with previous literature, and indicates that the PLGA-SBC porous microparticle substrates are promising for in vitro tumor or tissue development.</description><subject>Bioengineering</subject><subject>Biology and Life Sciences</subject><subject>Biomedical engineering</subject><subject>Biotechnology</subject><subject>Cell adhesion</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Count</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell death</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell size</subject><subject>Cell Survival - drug effects</subject><subject>Cell-Derived Microparticles - drug effects</subject><subject>Comparative studies</subject><subject>Culture techniques</subject><subject>Diffusion</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Drug resistance</subject><subject>Drug Resistance, Neoplasm - drug effects</subject><subject>Drug screening</subject><subject>Extracellular matrix</subject><subject>Fibronectin</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>Gelatin - pharmacology</subject><subject>Glycolic acid</subject><subject>Humans</subject><subject>Isopropylacrylamide</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Lung Neoplasms - drug therapy</subject><subject>Lung Neoplasms - pathology</subject><subject>Medicine and Health Sciences</subject><subject>Metabolism</subject><subject>Methods</subject><subject>Microparticles</subject><subject>Nutrients</subject><subject>Oxygen</subject><subject>Polylactic acid</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology</subject><subject>Polylactide-co-glycolide</subject><subject>Pores</subject><subject>Porosity</subject><subject>Research and Analysis Methods</subject><subject>Scaffolds</subject><subject>Sodium</subject><subject>Sodium bicarbonate</subject><subject>Spheroids</subject><subject>Substrates</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - 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drug effects</topic><topic>Cell Count</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell death</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell size</topic><topic>Cell Survival - drug effects</topic><topic>Cell-Derived Microparticles - drug effects</topic><topic>Comparative studies</topic><topic>Culture techniques</topic><topic>Diffusion</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Drug resistance</topic><topic>Drug Resistance, Neoplasm - drug effects</topic><topic>Drug screening</topic><topic>Extracellular matrix</topic><topic>Fibronectin</topic><topic>Gelatin</topic><topic>Gelatin - chemistry</topic><topic>Gelatin - pharmacology</topic><topic>Glycolic acid</topic><topic>Humans</topic><topic>Isopropylacrylamide</topic><topic>Lung cancer</topic><topic>Lung diseases</topic><topic>Lung Neoplasms - drug therapy</topic><topic>Lung Neoplasms - pathology</topic><topic>Medicine and Health Sciences</topic><topic>Metabolism</topic><topic>Methods</topic><topic>Microparticles</topic><topic>Nutrients</topic><topic>Oxygen</topic><topic>Polylactic acid</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuriakose, Aneetta E</au><au>Hu, Wenjing</au><au>Nguyen, Kytai T</au><au>Menon, Jyothi U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scaffold-based lung tumor culture on porous PLGA microparticle substrates</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2019-05-31</date><risdate>2019</risdate><volume>14</volume><issue>5</issue><spage>e0217640</spage><epage>e0217640</epage><pages>e0217640-e0217640</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Competing Interests: The commercial affiliation for WH with Progenitec Inc does not alter our adherence to PLOS ONE policies on sharing data and materials. There are no patents, or products in development or in the market to declare.</notes><abstract>Scaffold-based cancer cell culture techniques have been gaining prominence especially in the last two decades. These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and uncontrollable size of cell aggregates. Porous scaffolds can provide a convenient support for cell attachment, proliferation and migration, and also allows diffusion of oxygen, nutrients and waste. In this paper, a comparative study was done on porous poly (lactic-co-glycolic acid) (PLGA) microparticles prepared using three porogens-gelatin, sodium bicarbonate (SBC) or novel poly N-isopropylacrylamide [PNIPAAm] particles, as substrates for lung cancer cell culture. These fibronectin-coated, stable particles (19-42 μm) supported A549 cell attachment at an optimal cell seeding density of 250,000 cells/ mg of particles. PLGA-SBC porous particles had comparatively larger, more interconnected pores, and favored greater cell proliferation up to 9 days than their counterparts. This indicates that pore diameters and interconnectivity have direct implications on scaffold-based cell culture compared to substrates with minimally interconnected pores (PLGA-gelatin) or pores of uniform sizes (PLGA-PMPs). Therefore, PLGA-SBC-based tumor models were chosen for preliminary drug screening studies. The greater drug resistance observed in the lung cancer cells grown on porous particles compared to conventional cell monolayers agrees with previous literature, and indicates that the PLGA-SBC porous microparticle substrates are promising for in vitro tumor or tissue development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>31150477</pmid><doi>10.1371/journal.pone.0217640</doi><orcidid>https://orcid.org/0000-0002-0762-3513</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-05, Vol.14 (5), p.e0217640-e0217640 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_2233259931 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Bioengineering Biology and Life Sciences Biomedical engineering Biotechnology Cell adhesion Cell Adhesion - drug effects Cell Count Cell culture Cell Culture Techniques Cell death Cell migration Cell proliferation Cell Proliferation - drug effects Cell size Cell Survival - drug effects Cell-Derived Microparticles - drug effects Comparative studies Culture techniques Diffusion Drug Evaluation, Preclinical - methods Drug resistance Drug Resistance, Neoplasm - drug effects Drug screening Extracellular matrix Fibronectin Gelatin Gelatin - chemistry Gelatin - pharmacology Glycolic acid Humans Isopropylacrylamide Lung cancer Lung diseases Lung Neoplasms - drug therapy Lung Neoplasms - pathology Medicine and Health Sciences Metabolism Methods Microparticles Nutrients Oxygen Polylactic acid Polylactic Acid-Polyglycolic Acid Copolymer - pharmacology Polylactide-co-glycolide Pores Porosity Research and Analysis Methods Scaffolds Sodium Sodium bicarbonate Spheroids Substrates Tissue Engineering Tissue Scaffolds - chemistry Tumors |
| title | Scaffold-based lung tumor culture on porous PLGA microparticle substrates |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T09%3A52%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Scaffold-based%20lung%20tumor%20culture%20on%20porous%20PLGA%20microparticle%20substrates&rft.jtitle=PloS%20one&rft.au=Kuriakose,%20Aneetta%20E&rft.date=2019-05-31&rft.volume=14&rft.issue=5&rft.spage=e0217640&rft.epage=e0217640&rft.pages=e0217640-e0217640&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0217640&rft_dat=%3Cproquest_plos_%3E2233259931%3C/proquest_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c526t-cfc010b6c010621d9512e81dae0ea11dbe0fb979ca415b0647cc216398d9ffb33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2233259931&rft_id=info:pmid/31150477&rfr_iscdi=true |