Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology

Eimeria species cause the intestinal disease coccidiosis, most notably in poultry. While the direct impact of coccidiosis on animal health and welfare is clear, its influence on the enteric microbiota and by-stander effects on chicken health and production remains largely unknown, with the possible...

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Published in:PloS one 2017-09, Vol.12 (9), p.e0184890-e0184890
Main Authors: Macdonald, Sarah E, Nolan, Matthew J, Harman, Kimberley, Boulton, Kay, Hume, David A, Tomley, Fiona M, Stabler, Richard A, Blake, Damer P
Format: Article
Language:English
Subjects:
Abundance
Age
Animal health
Animals
Bacteria
Biodiversity
Biology and Life Sciences
Bird Diseases - microbiology
Bird Diseases - pathology
Birds
Broilers (Chickens)
Causes of
Cecum - microbiology
Cecum - pathology
Change detection
Chickens
Chickens - microbiology
Coccidiosis
Coccidiosis - microbiology
Coccidiosis - pathology
Coccidiosis - veterinary
Development and progression
Diseases and pests
Drug resistance
Dysbacteriosis
E coli
Eimeria tenella
Enteritis
Gastrointestinal Microbiome - genetics
Genotype & phenotype
Infections
Inflammatory bowel disease
Intervention
Intestine
Lesions
Medicine and Health Sciences
Microbiota (Symbiotic organisms)
Microorganisms
Parasites
Pathology
Phylogeny
Physiological aspects
Population
Poultry
Probiotics
Random Allocation
RNA, Ribosomal, 16S - genetics
rRNA 16S
Salmonella
Sequence Analysis, DNA
Severity of Illness Index
Species diversity
Taxa
Veterinary colleges
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cited_by cdi_FETCH-LOGICAL-c692t-7c9aeb1298c0414fbe5cef98fc6731978bb6d8d2c377fec5b97e8293e99eb6543
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container_issue 9
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creator Macdonald, Sarah E
Nolan, Matthew J
Harman, Kimberley
Boulton, Kay
Hume, David A
Tomley, Fiona M
Stabler, Richard A
Blake, Damer P
description Eimeria species cause the intestinal disease coccidiosis, most notably in poultry. While the direct impact of coccidiosis on animal health and welfare is clear, its influence on the enteric microbiota and by-stander effects on chicken health and production remains largely unknown, with the possible exception of Clostridium perfringens (necrotic enteritis). This study evaluated the composition and structure of the caecal microbiome in the presence or absence of a defined Eimeria tenella challenge infection in Cobb500 broiler chickens using 16S rRNA amplicon sequencing. The severity of clinical coccidiosis in individual chickens was quantified by caecal lesion scoring and microbial changes associated with different lesion scores identified. Following E. tenella infection the diversity of taxa within the caecal microbiome remained largely stable. However, infection induced significant changes in the abundance of some microbial taxa. The greatest changes were detected in birds displaying severe caecal pathology; taxa belonging to the order Enterobacteriaceae were increased, while taxa from Bacillales and Lactobacillales were decreased with the changes correlated with lesion severity. Significantly different profiles were also detected in infected birds which remained asymptomatic (lesion score 0), with taxa belonging to the genera Bacteroides decreased and Lactobacillus increased. Many differential taxa from the order Clostridiales were identified, with some increasing and others decreasing in abundance in Eimeria-infected animals. The results support the view that caecal microbiome dysbiosis associated with Eimeria infection contributes to disease pathology, and could be a target for intervention to mitigate the impact of coccidiosis on poultry productivity and welfare. This work highlights that E. tenella infection has a significant impact on the abundance of some caecal bacteria with notable differences detected between lesion score categories emphasising the importance of accounting for differences in caecal lesions when investigating the relationship between E. tenella and the poultry intestinal microbiome.
doi_str_mv 10.1371/journal.pone.0184890
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Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Macdonald, Sarah E</au><au>Nolan, Matthew J</au><au>Harman, Kimberley</au><au>Boulton, Kay</au><au>Hume, David A</au><au>Tomley, Fiona M</au><au>Stabler, Richard A</au><au>Blake, Damer P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-09-21</date><risdate>2017</risdate><volume>12</volume><issue>9</issue><spage>e0184890</spage><epage>e0184890</epage><pages>e0184890-e0184890</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Competing Interests: The authors have declared that no competing interests exist.</notes><abstract>Eimeria species cause the intestinal disease coccidiosis, most notably in poultry. While the direct impact of coccidiosis on animal health and welfare is clear, its influence on the enteric microbiota and by-stander effects on chicken health and production remains largely unknown, with the possible exception of Clostridium perfringens (necrotic enteritis). This study evaluated the composition and structure of the caecal microbiome in the presence or absence of a defined Eimeria tenella challenge infection in Cobb500 broiler chickens using 16S rRNA amplicon sequencing. The severity of clinical coccidiosis in individual chickens was quantified by caecal lesion scoring and microbial changes associated with different lesion scores identified. Following E. tenella infection the diversity of taxa within the caecal microbiome remained largely stable. However, infection induced significant changes in the abundance of some microbial taxa. The greatest changes were detected in birds displaying severe caecal pathology; taxa belonging to the order Enterobacteriaceae were increased, while taxa from Bacillales and Lactobacillales were decreased with the changes correlated with lesion severity. Significantly different profiles were also detected in infected birds which remained asymptomatic (lesion score 0), with taxa belonging to the genera Bacteroides decreased and Lactobacillus increased. Many differential taxa from the order Clostridiales were identified, with some increasing and others decreasing in abundance in Eimeria-infected animals. The results support the view that caecal microbiome dysbiosis associated with Eimeria infection contributes to disease pathology, and could be a target for intervention to mitigate the impact of coccidiosis on poultry productivity and welfare. This work highlights that E. tenella infection has a significant impact on the abundance of some caecal bacteria with notable differences detected between lesion score categories emphasising the importance of accounting for differences in caecal lesions when investigating the relationship between E. tenella and the poultry intestinal microbiome.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28934262</pmid><doi>10.1371/journal.pone.0184890</doi><tpages>e0184890</tpages><orcidid>https://orcid.org/0000-0003-4710-0191</orcidid><oa>free_for_read</oa></addata></record>
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source Open Access: PubMed Central; ProQuest - Publicly Available Content Database
subjects Abundance
Age
Animal health
Animals
Bacteria
Biodiversity
Biology and Life Sciences
Bird Diseases - microbiology
Bird Diseases - pathology
Birds
Broilers (Chickens)
Causes of
Cecum - microbiology
Cecum - pathology
Change detection
Chickens
Chickens - microbiology
Coccidiosis
Coccidiosis - microbiology
Coccidiosis - pathology
Coccidiosis - veterinary
Development and progression
Diseases and pests
Drug resistance
Dysbacteriosis
E coli
Eimeria tenella
Enteritis
Gastrointestinal Microbiome - genetics
Genotype & phenotype
Infections
Inflammatory bowel disease
Intervention
Intestine
Lesions
Medicine and Health Sciences
Microbiota (Symbiotic organisms)
Microorganisms
Parasites
Pathology
Phylogeny
Physiological aspects
Population
Poultry
Probiotics
Random Allocation
RNA, Ribosomal, 16S - genetics
rRNA 16S
Salmonella
Sequence Analysis, DNA
Severity of Illness Index
Species diversity
Taxa
Veterinary colleges
title Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology
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