Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil

The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in...

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Bibliographic Details
Published in:PloS one 2016-01, Vol.11 (1), p.e0145954
Main Authors: Machado, Bruna Aparecida Souza, Silva, Rejane Pina Dantas, Barreto, Gabriele de Abreu, Costa, Samantha Serra, Silva, Danielle Figuerêdo da, Brandão, Hugo Neves, Rocha, José Luiz Carneiro da, Dellagostin, Odir Antônio, Henriques, João Antônio Pegas, Umsza-Guez, Marcelo Andres, Padilha, Francine Ferreira
Format: Article
Language:English
Subjects:
Acids
Animals
Anti-Infective Agents - chemistry
Anti-Infective Agents - isolation & purification
Anti-Infective Agents - pharmacology
Antimicrobial activity
Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - isolation & purification
Antineoplastic Agents, Phytogenic - toxicity
Antioxidants
Antioxidants - chemistry
Antioxidants - isolation & purification
Antioxidants - pharmacology
Apoptosis
Bacteria
Biological activity
Brazil
Breast cancer
Cell Line, Tumor
Cell lines
Cell Proliferation - drug effects
Chemical composition
Chemical properties
Chromatography, High Pressure Liquid
Chromatography, Supercritical Fluid
Comparative analysis
Coumaric acid
Coumaric Acids - chemistry
Coumaric Acids - isolation & purification
Coumaric Acids - pharmacology
Escherichia coli - drug effects
Ethanol
Ethanol - chemistry
Extraction (Chemistry)
Flavonoids
Flavonoids - chemistry
Flavonoids - isolation & purification
Flavonoids - pharmacology
Food
Kinases
Mice
Microscopy, Electron, Scanning
p-Coumaric acid
Pharmacy
Phenolic compounds
Phenols
Phenols - chemistry
Phenols - isolation & purification
Phenols - pharmacology
Phenylpropionates - chemistry
Phenylpropionates - isolation & purification
Phenylpropionates - pharmacology
Physiological aspects
Propionates
Propolis
Propolis - analysis
Propolis - isolation & purification
Propolis - pharmacology
Prostate cancer
Staphylococcus aureus - drug effects
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Summary:The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in eight samples of different types of propolis (red, green and brown), collected from different regions in Brazil. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH and ABTS), Artepillin C, p-coumaric acid and antimicrobial activity against two bacteria were determined for all extracts. For the EtOH extracts, the anti-proliferative activity regarding the cell lines of B16F10, were also evaluated. Amongst the samples evaluated, the red propolis from the Brazilian Northeast (states of Sergipe and Alagoas) showed the higher biological potential, as well as the larger content of antioxidant compounds. The best results were shown for the extracts obtained through the conventional extraction method (EtOH). However, the highest concentrations of Artepillin C and p-coumaric acid were identified in the extracts from SCO2, indicating a higher selectivity for the extraction of these compounds. It was verified that the composition and biological activity of the Brazilian propolis vary significantly, depending on the type of sample and geographical area of collection.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0145954