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Molecular epidemiology of seal parvovirus, 1988-2014
A novel parvovirus was discovered recently in the brain of a harbor seal (Phoca vitulina) with chronic meningo-encephalitis. Phylogenetic analysis of this virus indicated that it belongs to the genus Erythroparvovirus, to which also human parvovirus B19 belongs. In the present study, the prevalence,...
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| Published in: | PloS one 2014-11, Vol.9 (11), p.e112129-e112129 |
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| creator | Bodewes, Rogier Hapsari, Rebriarina Rubio García, Ana Sánchez Contreras, Guillermo J van de Bildt, Marco W G de Graaf, Miranda Kuiken, Thijs Osterhaus, Albert D M E |
| description | A novel parvovirus was discovered recently in the brain of a harbor seal (Phoca vitulina) with chronic meningo-encephalitis. Phylogenetic analysis of this virus indicated that it belongs to the genus Erythroparvovirus, to which also human parvovirus B19 belongs. In the present study, the prevalence, genetic diversity and clinical relevance of seal parvovirus (SePV) infections was evaluated in both harbor and grey seals (Halichoerus grypus) that lived in Northwestern European coastal waters from 1988 to 2014. To this end, serum and tissue samples collected from seals were tested for the presence of seal parvovirus DNA by real-time PCR and the sequences of the partial NS gene and the complete VP2 gene of positive samples were determined. Seal parvovirus DNA was detected in nine (8%) of the spleen tissues tested and in one (0.5%) of the serum samples tested, including samples collected from seals that died in 1988. Sequence analysis of the partial NS and complete VP2 genes of nine SePV revealed multiple sites with nucleotide substitutions but only one amino acid change in the VP2 gene. Estimated nucleotide substitution rates per year were 2.00 × 10(-4) for the partial NS gene and 1.15 × 10(-4) for the complete VP2 gene. Most samples containing SePV DNA were co-infected with phocine herpesvirus 1 or PDV, so no conclusions could be drawn about the clinical impact of SePV infection alone. The present study is one of the few in which the mutation rates of parvoviruses were evaluated over a period of more than 20 years, especially in a wildlife population, providing additional insights into the genetic diversity of parvoviruses. |
| doi_str_mv | 10.1371/journal.pone.0112129 |
| format | article |
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Phylogenetic analysis of this virus indicated that it belongs to the genus Erythroparvovirus, to which also human parvovirus B19 belongs. In the present study, the prevalence, genetic diversity and clinical relevance of seal parvovirus (SePV) infections was evaluated in both harbor and grey seals (Halichoerus grypus) that lived in Northwestern European coastal waters from 1988 to 2014. To this end, serum and tissue samples collected from seals were tested for the presence of seal parvovirus DNA by real-time PCR and the sequences of the partial NS gene and the complete VP2 gene of positive samples were determined. Seal parvovirus DNA was detected in nine (8%) of the spleen tissues tested and in one (0.5%) of the serum samples tested, including samples collected from seals that died in 1988. Sequence analysis of the partial NS and complete VP2 genes of nine SePV revealed multiple sites with nucleotide substitutions but only one amino acid change in the VP2 gene. Estimated nucleotide substitution rates per year were 2.00 × 10(-4) for the partial NS gene and 1.15 × 10(-4) for the complete VP2 gene. Most samples containing SePV DNA were co-infected with phocine herpesvirus 1 or PDV, so no conclusions could be drawn about the clinical impact of SePV infection alone. The present study is one of the few in which the mutation rates of parvoviruses were evaluated over a period of more than 20 years, especially in a wildlife population, providing additional insights into the genetic diversity of parvoviruses.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0112129</identifier><identifier>PMID: 25390639</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Amino acid substitution ; Analysis ; Animals ; Atlantic Ocean ; Base Sequence ; Biology and Life Sciences ; Blood diseases ; Bone marrow ; Brain ; Brain - virology ; Brain research ; Capsid Proteins - genetics ; Coastal waters ; Deoxyribonucleic acid ; Disease ; DNA ; DNA, Viral - analysis ; Encephalitis ; Epidemics ; Epidemiology ; Europe - epidemiology ; Evolution ; Gene sequencing ; Genetic diversity ; Genome, Viral ; Grey seals ; Health aspects ; Infections ; Marine mammals ; Medicine and Health Sciences ; Meningoencephalitis - epidemiology ; Meningoencephalitis - veterinary ; Meningoencephalitis - virology ; Molecular Epidemiology ; Monkeys & apes ; Mutation ; Mutation rates ; Nucleotide sequence ; Parvovirus - classification ; Parvovirus - genetics ; Parvoviruses ; Phoca - virology ; Phoca vitulina ; Phylogenetics ; Phylogeny ; Prevalence ; Real-Time Polymerase Chain Reaction ; Rehabilitation ; Seals ; Sequence Analysis, DNA ; Spleen ; Tissues ; Veterinary medicine ; Viral Nonstructural Proteins - chemistry ; Viral Nonstructural Proteins - genetics ; Viruses ; Wildlife</subject><ispartof>PloS one, 2014-11, Vol.9 (11), p.e112129-e112129</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Bodewes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Bodewes et al 2014 Bodewes et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a3470da9709c960d2e70018ee961d3fd8ae2b2e944a252e5b9bbc6aa9a62640c3</citedby><cites>FETCH-LOGICAL-c692t-a3470da9709c960d2e70018ee961d3fd8ae2b2e944a252e5b9bbc6aa9a62640c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1623314313/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1623314313?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,37048,44625,53827,53829,75483</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25390639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Qiu, Jianming</contributor><creatorcontrib>Bodewes, Rogier</creatorcontrib><creatorcontrib>Hapsari, Rebriarina</creatorcontrib><creatorcontrib>Rubio García, Ana</creatorcontrib><creatorcontrib>Sánchez Contreras, Guillermo J</creatorcontrib><creatorcontrib>van de Bildt, Marco W G</creatorcontrib><creatorcontrib>de Graaf, Miranda</creatorcontrib><creatorcontrib>Kuiken, Thijs</creatorcontrib><creatorcontrib>Osterhaus, Albert D M E</creatorcontrib><title>Molecular epidemiology of seal parvovirus, 1988-2014</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A novel parvovirus was discovered recently in the brain of a harbor seal (Phoca vitulina) with chronic meningo-encephalitis. Phylogenetic analysis of this virus indicated that it belongs to the genus Erythroparvovirus, to which also human parvovirus B19 belongs. In the present study, the prevalence, genetic diversity and clinical relevance of seal parvovirus (SePV) infections was evaluated in both harbor and grey seals (Halichoerus grypus) that lived in Northwestern European coastal waters from 1988 to 2014. To this end, serum and tissue samples collected from seals were tested for the presence of seal parvovirus DNA by real-time PCR and the sequences of the partial NS gene and the complete VP2 gene of positive samples were determined. Seal parvovirus DNA was detected in nine (8%) of the spleen tissues tested and in one (0.5%) of the serum samples tested, including samples collected from seals that died in 1988. Sequence analysis of the partial NS and complete VP2 genes of nine SePV revealed multiple sites with nucleotide substitutions but only one amino acid change in the VP2 gene. Estimated nucleotide substitution rates per year were 2.00 × 10(-4) for the partial NS gene and 1.15 × 10(-4) for the complete VP2 gene. Most samples containing SePV DNA were co-infected with phocine herpesvirus 1 or PDV, so no conclusions could be drawn about the clinical impact of SePV infection alone. The present study is one of the few in which the mutation rates of parvoviruses were evaluated over a period of more than 20 years, especially in a wildlife population, providing additional insights into the genetic diversity of parvoviruses.</description><subject>Age</subject><subject>Amino acid substitution</subject><subject>Analysis</subject><subject>Animals</subject><subject>Atlantic Ocean</subject><subject>Base Sequence</subject><subject>Biology and Life Sciences</subject><subject>Blood diseases</subject><subject>Bone marrow</subject><subject>Brain</subject><subject>Brain - virology</subject><subject>Brain research</subject><subject>Capsid Proteins - genetics</subject><subject>Coastal waters</subject><subject>Deoxyribonucleic acid</subject><subject>Disease</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>Encephalitis</subject><subject>Epidemics</subject><subject>Epidemiology</subject><subject>Europe - epidemiology</subject><subject>Evolution</subject><subject>Gene sequencing</subject><subject>Genetic diversity</subject><subject>Genome, Viral</subject><subject>Grey seals</subject><subject>Health aspects</subject><subject>Infections</subject><subject>Marine mammals</subject><subject>Medicine and Health Sciences</subject><subject>Meningoencephalitis - epidemiology</subject><subject>Meningoencephalitis - veterinary</subject><subject>Meningoencephalitis - virology</subject><subject>Molecular Epidemiology</subject><subject>Monkeys & apes</subject><subject>Mutation</subject><subject>Mutation rates</subject><subject>Nucleotide sequence</subject><subject>Parvovirus - classification</subject><subject>Parvovirus - genetics</subject><subject>Parvoviruses</subject><subject>Phoca - virology</subject><subject>Phoca vitulina</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Prevalence</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Rehabilitation</subject><subject>Seals</subject><subject>Sequence Analysis, DNA</subject><subject>Spleen</subject><subject>Tissues</subject><subject>Veterinary medicine</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Viruses</subject><subject>Wildlife</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QHBFGwY3KSpsmNsCx-DKws-HUb0jTtZMg0NWkH99-b2ekuU9kLyUXCyXPek3PyZtlzjJaYlPj9xo-hU27Z-84sEcaAQTzITrEgkDNA5OHR-SR7EuMGoYJwxh5nJ1AQgRgRpxn96p3Ro1NhYXpbm631zrfXC98solFu0auw8zsbxvhugQXnOSBMn2aPGuWieTbtZ9nPTx9_XHzJL68-ry7OL3PNBAy5IrREtRIlElowVIMpEcLcGMFwTZqaKwMVGEGpggJMUYmq0kwpoRgwijQ5y14edHvno5wajhIzIARTgkkiVgei9moj-2C3KlxLr6y8CfjQShUGq52R0EANggNntKJVyTggUXNU4KrmDaAyaX2Yqo3V1tTadENQbiY6v-nsWrZ-JymASONPAm8mgeB_jyYOcmujNs6pzvjx5t0FoZRwmtBX_6D3dzdRrUoN2K7xqa7ei8pzinlRQElRopb3UGntf1MndzQ2xWcJb2cJiRnMn6FVY4xy9f3b_7NXv-bs6yN2newzrKN342B9F-cgPYA6-BiDae6GjJHcm_t2GnJvbjmZO6W9OP6gu6RbN5O_a4zwIQ</recordid><startdate>20141112</startdate><enddate>20141112</enddate><creator>Bodewes, Rogier</creator><creator>Hapsari, Rebriarina</creator><creator>Rubio García, Ana</creator><creator>Sánchez Contreras, Guillermo J</creator><creator>van de Bildt, Marco W G</creator><creator>de Graaf, Miranda</creator><creator>Kuiken, Thijs</creator><creator>Osterhaus, Albert D M E</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141112</creationdate><title>Molecular epidemiology of seal parvovirus, 1988-2014</title><author>Bodewes, Rogier ; Hapsari, Rebriarina ; Rubio García, Ana ; Sánchez Contreras, Guillermo J ; van de Bildt, Marco W G ; de Graaf, Miranda ; Kuiken, Thijs ; Osterhaus, Albert D M E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a3470da9709c960d2e70018ee961d3fd8ae2b2e944a252e5b9bbc6aa9a62640c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Age</topic><topic>Amino acid substitution</topic><topic>Analysis</topic><topic>Animals</topic><topic>Atlantic Ocean</topic><topic>Base Sequence</topic><topic>Biology and Life Sciences</topic><topic>Blood diseases</topic><topic>Bone marrow</topic><topic>Brain</topic><topic>Brain - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodewes, Rogier</au><au>Hapsari, Rebriarina</au><au>Rubio García, Ana</au><au>Sánchez Contreras, Guillermo J</au><au>van de Bildt, Marco W G</au><au>de Graaf, Miranda</au><au>Kuiken, Thijs</au><au>Osterhaus, Albert D M E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of seal parvovirus, 1988-2014</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-11-12</date><risdate>2014</risdate><volume>9</volume><issue>11</issue><spage>e112129</spage><epage>e112129</epage><pages>e112129-e112129</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Conceived and designed the experiments: RB RH MdG ADMEO. Performed the experiments: RB RH ARG GJSC MWGvdB MdG. Analyzed the data: RB RH MdG. Contributed reagents/materials/analysis tools: ARG GJSC MWGvdB TK. Wrote the paper: RB RH MdG TK ADMEO.</notes><notes>Competing Interests: One author of the manuscript has interests to declare: Prof. Dr. ADME Osterhaus is part time chief scientific officer of Viroclinics Biosciences B.V. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials. The other authors have no competing interests to declare.</notes><abstract>A novel parvovirus was discovered recently in the brain of a harbor seal (Phoca vitulina) with chronic meningo-encephalitis. Phylogenetic analysis of this virus indicated that it belongs to the genus Erythroparvovirus, to which also human parvovirus B19 belongs. In the present study, the prevalence, genetic diversity and clinical relevance of seal parvovirus (SePV) infections was evaluated in both harbor and grey seals (Halichoerus grypus) that lived in Northwestern European coastal waters from 1988 to 2014. To this end, serum and tissue samples collected from seals were tested for the presence of seal parvovirus DNA by real-time PCR and the sequences of the partial NS gene and the complete VP2 gene of positive samples were determined. Seal parvovirus DNA was detected in nine (8%) of the spleen tissues tested and in one (0.5%) of the serum samples tested, including samples collected from seals that died in 1988. Sequence analysis of the partial NS and complete VP2 genes of nine SePV revealed multiple sites with nucleotide substitutions but only one amino acid change in the VP2 gene. Estimated nucleotide substitution rates per year were 2.00 × 10(-4) for the partial NS gene and 1.15 × 10(-4) for the complete VP2 gene. Most samples containing SePV DNA were co-infected with phocine herpesvirus 1 or PDV, so no conclusions could be drawn about the clinical impact of SePV infection alone. The present study is one of the few in which the mutation rates of parvoviruses were evaluated over a period of more than 20 years, especially in a wildlife population, providing additional insights into the genetic diversity of parvoviruses.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25390639</pmid><doi>10.1371/journal.pone.0112129</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-11, Vol.9 (11), p.e112129-e112129 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1623314313 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Age Amino acid substitution Analysis Animals Atlantic Ocean Base Sequence Biology and Life Sciences Blood diseases Bone marrow Brain Brain - virology Brain research Capsid Proteins - genetics Coastal waters Deoxyribonucleic acid Disease DNA DNA, Viral - analysis Encephalitis Epidemics Epidemiology Europe - epidemiology Evolution Gene sequencing Genetic diversity Genome, Viral Grey seals Health aspects Infections Marine mammals Medicine and Health Sciences Meningoencephalitis - epidemiology Meningoencephalitis - veterinary Meningoencephalitis - virology Molecular Epidemiology Monkeys & apes Mutation Mutation rates Nucleotide sequence Parvovirus - classification Parvovirus - genetics Parvoviruses Phoca - virology Phoca vitulina Phylogenetics Phylogeny Prevalence Real-Time Polymerase Chain Reaction Rehabilitation Seals Sequence Analysis, DNA Spleen Tissues Veterinary medicine Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - genetics Viruses Wildlife |
| title | Molecular epidemiology of seal parvovirus, 1988-2014 |
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