Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil

St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2013-11, Vol.7 (11), p.e2537-e2537
Main Authors: Rosa, Roberta, Costa, Erica Azevedo, Marques, Rafael Elias, Oliveira, Taismara Simas, Furtini, Ronaldo, Bomfim, Maria Rosa Quaresma, Teixeira, Mauro Martins, Paixão, Tatiane Alves, Santos, Renato Lima
Format: Article
Language:English
Subjects:
Animals
Bird migration
Brain - virology
Brazil
Colleges & universities
Dengue fever
Dengue virus
Encephalitis
Encephalitis Virus, St. Louis - genetics
Encephalitis Virus, St. Louis - isolation & purification
Encephalitis, St. Louis - veterinary
Encephalitis, St. Louis - virology
Experiments
Flavivirus
Fluorescent Antibody Technique, Indirect
Genotype & phenotype
Horse Diseases - virology
Horses
Immunization
Japanese encephalitis virus
Mice
Molecular Sequence Data
Mortality
Phylogenetics
Physiological aspects
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - genetics
Sequence Analysis, DNA
West Nile virus
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Description
Summary:St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0002537