Anti-viral immune response in the lung and thymus: Molecular characterization and expression analysis of immunoproteasome subunits LMP2, LMP7 and MECL-1 in pigs

Both the lung and the thymus are vital target organ for pathogens including viruses. The immunoproteasome (i-proteasome) enhances antigen presentation for MHC class I molecules to activate CD8+T lymphocyte. These facilitate antiviral adaptive immune response. Our previous study found that, expressio...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-08, Vol.502 (4), p.472-478
Main Authors: Liu, Qiang, Hu, Wei, Zhang, Yong-Li, Hu, Shou-Ping, Zhang, Zhuo, He, Xi-Jun, Cai, Xue-Hui
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
AMINO ACIDS
CYTOPLASM
Immunoproteasome
INFLAMMATION
LMP2
LMP7
LUNGS
LYMPHOCYTES
MECL-1
Pig
PROTEINS
SWINE
THYMUS
VIRUSES
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Summary:Both the lung and the thymus are vital target organ for pathogens including viruses. The immunoproteasome (i-proteasome) enhances antigen presentation for MHC class I molecules to activate CD8+T lymphocyte. These facilitate antiviral adaptive immune response. Our previous study found that, expression of i-proteasome subunits in porcine lung was altered during normal and inflammatory conditions. To date, the expression of i-proteasome subunits in porcine thymus to viruses has not been investigated. In the present study, LMP2, LMP7, and MECL-1 were cloned, identified and their sequences encoded predicted proteins of 216, 275, and 278 amino acids, respectively. Expression of LMP2, LMP7, and MECL-1, in the cytoplasm and nucleus, was markedly altered in the porcine reproductive and respiratory syndrome virus (PRRSV)-infected lung and thymus. And dendritic cells and epithelial cells readily expressed the i-proteasome subunit LMP2 in the thymus of PRRSV-infected pigs compared to that in mock-infected pigs. Additionally, the in vitro stimulation of a PAM cell line with PolyI:C for 12 and 24 h resulted in increased LMP2, LMP7, and MECL-1 expression. These results suggest a central role for these complexes in the activation of an antiviral immune response in pigs. A better understanding of the role of the i-proteasome in different cell types, tissues, and hosts could improve vaccine design and facilitate the development of effective treatment strategies for viral infections.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.05.190