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Tsc1-dependent transcriptional programming of dendritic cell homeostasis and function
Dendritic cells (DCs) are pivotal to initiating adaptive immune response. Emerging evidence highlights important roles of tuberous sclerosis complex 1 (Tsc1) in DC development and activation. Our previous study also showed that Tsc1 expression in DCs was required to promote T-cell homeostasis and re...
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| Published in: | Experimental cell research 2018-02, Vol.363 (1), p.73-83 |
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| Main Authors: | , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c387t-6ba7ec76c2b2153bd7558dfab31902fc79e049b05e2cfa4013bc4b9e940692cf3 |
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| cites | cdi_FETCH-LOGICAL-c387t-6ba7ec76c2b2153bd7558dfab31902fc79e049b05e2cfa4013bc4b9e940692cf3 |
| container_end_page | 83 |
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| container_start_page | 73 |
| container_title | Experimental cell research |
| container_volume | 363 |
| creator | Luo, Yuechen Liu, Jingru Sun, Xiaolei Feng, Tiantian Fang, Lijun Chen, Song Fang, Chunmin Feng, Xiaoming Huang, Huifang |
| description | Dendritic cells (DCs) are pivotal to initiating adaptive immune response. Emerging evidence highlights important roles of tuberous sclerosis complex 1 (Tsc1) in DC development and activation. Our previous study also showed that Tsc1 expression in DCs was required to promote T-cell homeostasis and response partially through inhibiting mammalian target of rapamycin complex1 (mTORC1). However, the molecular mechanism of transcriptional regulation by which Tsc1 control DC homeostasis and function remains largely unknown. Here we globally identified the Tsc1-regulated genes by comparing the transcriptional profiling of Tsc1-deficient DCs with wild-type DCs. It showed that Tsc1 specifically regulated the expression of groups of gene sets critically involved in DC survival, proliferation, metabolism and antigen presentation. The impacts of Tsc1 on DC gene expression were partially dependent on inhibition of mTORC1 signal. Our study thus provides a comprehensive molecular basis for understanding how Tsc1 programs the homeostasis and function of DCs through transcriptional regulation.
•Transcriptional mechanisms of Tsc1-regulated DC homeostasis and function.•Tsc1 controls DC survival, proliferation, metabolism and antigen presentation.•Tsc1affect DC gene expression partially through inhibition of mTORC1 signal. |
| doi_str_mv | 10.1016/j.yexcr.2017.12.028 |
| format | article |
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•Transcriptional mechanisms of Tsc1-regulated DC homeostasis and function.•Tsc1 controls DC survival, proliferation, metabolism and antigen presentation.•Tsc1affect DC gene expression partially through inhibition of mTORC1 signal.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2017.12.028</identifier><identifier>PMID: 29294307</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; Antigen Presentation - immunology ; ANTIGENS ; CELL PROLIFERATION ; DENDRITES ; Dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; Gene Expression Regulation - drug effects ; GENES ; HOMEOSTASIS ; Homeostasis - drug effects ; Homeostasis - physiology ; Mammalian target of rapamycin ; Mechanistic Target of Rapamycin Complex 1 - antagonists & inhibitors ; METABOLISM ; Mice, Transgenic ; Multiprotein Complexes - metabolism ; Transcriptional regulation ; Tuberous sclerosis complex 1 ; Tumor Suppressor Proteins - genetics ; Tumor Suppressor Proteins - metabolism</subject><ispartof>Experimental cell research, 2018-02, Vol.363 (1), p.73-83</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-6ba7ec76c2b2153bd7558dfab31902fc79e049b05e2cfa4013bc4b9e940692cf3</citedby><cites>FETCH-LOGICAL-c387t-6ba7ec76c2b2153bd7558dfab31902fc79e049b05e2cfa4013bc4b9e940692cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29294307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/23082477$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Yuechen</creatorcontrib><creatorcontrib>Liu, Jingru</creatorcontrib><creatorcontrib>Sun, Xiaolei</creatorcontrib><creatorcontrib>Feng, Tiantian</creatorcontrib><creatorcontrib>Fang, Lijun</creatorcontrib><creatorcontrib>Chen, Song</creatorcontrib><creatorcontrib>Fang, Chunmin</creatorcontrib><creatorcontrib>Feng, Xiaoming</creatorcontrib><creatorcontrib>Huang, Huifang</creatorcontrib><title>Tsc1-dependent transcriptional programming of dendritic cell homeostasis and function</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Dendritic cells (DCs) are pivotal to initiating adaptive immune response. Emerging evidence highlights important roles of tuberous sclerosis complex 1 (Tsc1) in DC development and activation. Our previous study also showed that Tsc1 expression in DCs was required to promote T-cell homeostasis and response partially through inhibiting mammalian target of rapamycin complex1 (mTORC1). However, the molecular mechanism of transcriptional regulation by which Tsc1 control DC homeostasis and function remains largely unknown. Here we globally identified the Tsc1-regulated genes by comparing the transcriptional profiling of Tsc1-deficient DCs with wild-type DCs. It showed that Tsc1 specifically regulated the expression of groups of gene sets critically involved in DC survival, proliferation, metabolism and antigen presentation. The impacts of Tsc1 on DC gene expression were partially dependent on inhibition of mTORC1 signal. Our study thus provides a comprehensive molecular basis for understanding how Tsc1 programs the homeostasis and function of DCs through transcriptional regulation.
•Transcriptional mechanisms of Tsc1-regulated DC homeostasis and function.•Tsc1 controls DC survival, proliferation, metabolism and antigen presentation.•Tsc1affect DC gene expression partially through inhibition of mTORC1 signal.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>Antigen Presentation - immunology</subject><subject>ANTIGENS</subject><subject>CELL PROLIFERATION</subject><subject>DENDRITES</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>GENES</subject><subject>HOMEOSTASIS</subject><subject>Homeostasis - drug effects</subject><subject>Homeostasis - physiology</subject><subject>Mammalian target of rapamycin</subject><subject>Mechanistic Target of Rapamycin Complex 1 - antagonists & inhibitors</subject><subject>METABOLISM</subject><subject>Mice, Transgenic</subject><subject>Multiprotein Complexes - metabolism</subject><subject>Transcriptional regulation</subject><subject>Tuberous sclerosis complex 1</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - metabolism</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v1DAQhi0EokvhFyChSFy4JIwnThwfOKCKL6kSl_ZsOfak9WpjL7YX0X-PwxaOnEYaPa_n9cPYaw4dBz6-33cP9MumDoHLjmMHOD1hOw4KWhSIT9kOgItWTCgv2Iuc9wAwTXx8zi5QoRI9yB27vcmWt46OFByF0pRkQrbJH4uPwRyaY4p3yayrD3dNXJrKuOSLt42lw6G5jyvFXEz2uTHBNcsp2C34kj1bzCHTq8d5yW4_f7q5-tpef__y7erjdWv7SZZ2nI0kK0eLM_Khn50chsktZu65AlysVARCzTAQ2sUI4P1sxaxICRhVXfWX7O353VrC62x9IXtvYwhki8YeJhRSVurdmaqf-XGiXPTq89bfBIqnrLmaBI4j4lDR_ozaFHNOtOhj8qtJD5qD3qzrvf5jXW_WNUddrdfUm8cDp3kl9y_zV3MFPpwBqjJ-ekpbVwqWnE9bVRf9fw_8BknxlTY</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Luo, Yuechen</creator><creator>Liu, Jingru</creator><creator>Sun, Xiaolei</creator><creator>Feng, Tiantian</creator><creator>Fang, Lijun</creator><creator>Chen, Song</creator><creator>Fang, Chunmin</creator><creator>Feng, Xiaoming</creator><creator>Huang, Huifang</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20180201</creationdate><title>Tsc1-dependent transcriptional programming of dendritic cell homeostasis and function</title><author>Luo, Yuechen ; Liu, Jingru ; Sun, Xiaolei ; Feng, Tiantian ; Fang, Lijun ; Chen, Song ; Fang, Chunmin ; Feng, Xiaoming ; Huang, Huifang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-6ba7ec76c2b2153bd7558dfab31902fc79e049b05e2cfa4013bc4b9e940692cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>Antigen Presentation - immunology</topic><topic>ANTIGENS</topic><topic>CELL PROLIFERATION</topic><topic>DENDRITES</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>GENES</topic><topic>HOMEOSTASIS</topic><topic>Homeostasis - drug effects</topic><topic>Homeostasis - physiology</topic><topic>Mammalian target of rapamycin</topic><topic>Mechanistic Target of Rapamycin Complex 1 - antagonists & inhibitors</topic><topic>METABOLISM</topic><topic>Mice, Transgenic</topic><topic>Multiprotein Complexes - metabolism</topic><topic>Transcriptional regulation</topic><topic>Tuberous sclerosis complex 1</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumor Suppressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Yuechen</creatorcontrib><creatorcontrib>Liu, Jingru</creatorcontrib><creatorcontrib>Sun, Xiaolei</creatorcontrib><creatorcontrib>Feng, Tiantian</creatorcontrib><creatorcontrib>Fang, Lijun</creatorcontrib><creatorcontrib>Chen, Song</creatorcontrib><creatorcontrib>Fang, Chunmin</creatorcontrib><creatorcontrib>Feng, Xiaoming</creatorcontrib><creatorcontrib>Huang, Huifang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Yuechen</au><au>Liu, Jingru</au><au>Sun, Xiaolei</au><au>Feng, Tiantian</au><au>Fang, Lijun</au><au>Chen, Song</au><au>Fang, Chunmin</au><au>Feng, Xiaoming</au><au>Huang, Huifang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tsc1-dependent transcriptional programming of dendritic cell homeostasis and function</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>363</volume><issue>1</issue><spage>73</spage><epage>83</epage><pages>73-83</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Dendritic cells (DCs) are pivotal to initiating adaptive immune response. Emerging evidence highlights important roles of tuberous sclerosis complex 1 (Tsc1) in DC development and activation. Our previous study also showed that Tsc1 expression in DCs was required to promote T-cell homeostasis and response partially through inhibiting mammalian target of rapamycin complex1 (mTORC1). However, the molecular mechanism of transcriptional regulation by which Tsc1 control DC homeostasis and function remains largely unknown. Here we globally identified the Tsc1-regulated genes by comparing the transcriptional profiling of Tsc1-deficient DCs with wild-type DCs. It showed that Tsc1 specifically regulated the expression of groups of gene sets critically involved in DC survival, proliferation, metabolism and antigen presentation. The impacts of Tsc1 on DC gene expression were partially dependent on inhibition of mTORC1 signal. Our study thus provides a comprehensive molecular basis for understanding how Tsc1 programs the homeostasis and function of DCs through transcriptional regulation.
•Transcriptional mechanisms of Tsc1-regulated DC homeostasis and function.•Tsc1 controls DC survival, proliferation, metabolism and antigen presentation.•Tsc1affect DC gene expression partially through inhibition of mTORC1 signal.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29294307</pmid><doi>10.1016/j.yexcr.2017.12.028</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental cell research, 2018-02, Vol.363 (1), p.73-83 |
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| source | ScienceDirect Freedom Collection |
| subjects | 60 APPLIED LIFE SCIENCES Animals Antigen Presentation - immunology ANTIGENS CELL PROLIFERATION DENDRITES Dendritic cells Dendritic Cells - cytology Dendritic Cells - immunology Gene Expression Regulation - drug effects GENES HOMEOSTASIS Homeostasis - drug effects Homeostasis - physiology Mammalian target of rapamycin Mechanistic Target of Rapamycin Complex 1 - antagonists & inhibitors METABOLISM Mice, Transgenic Multiprotein Complexes - metabolism Transcriptional regulation Tuberous sclerosis complex 1 Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism |
| title | Tsc1-dependent transcriptional programming of dendritic cell homeostasis and function |
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