MOLECULAR CHARACTERIZATION OF POTYVIRUSES INFECTING POTATO AND VEGETABLES IN IRAQ

The genetic diversity of potyviruses infecting potato and vegetables in Iraq was determined by reverse transcription polymerase chain reaction (RT-PCR) targeting the 3 terminal part of the genome. RT-PCR products were cloned and sequenced. Sequence analysis confirmed the occurrence of potyviruses, n...

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Bibliographic Details
Published in:Journal of plant pathology 2016-01, Vol.98 (3), p.603-606
Main Authors: Al-Kuwaiti, N.A., Maruthi, M.N., Seal, S.E.
Format: Article
Language:English
Subjects:
Agriculture
Beans
Mosaic viruses
Phylogenetics
Phytopathology
Plants
Potyvirus
Short Communication
Vegetables
Virology
Viruses
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Summary:The genetic diversity of potyviruses infecting potato and vegetables in Iraq was determined by reverse transcription polymerase chain reaction (RT-PCR) targeting the 3 terminal part of the genome. RT-PCR products were cloned and sequenced. Sequence analysis confirmed the occurrence of potyviruses, namely Bean yellow mosaic virus (BYMV) in broad bean, Potato virus Y (PVY) in potato and tomato, and Zucchini yellow mosaic virus (ZYMV) in zucchini squash. BYMV sequences showed maximum nucleotide sequence identity (97.2-98.7%) with isolates from Australia and Japan. Most PVY sequences were very closely related (ca. 99% nucleotide sequence identity) to PVYO:N isolates from USA and Canada, whereas other sequences showed nucleotide identities (98.5-98.7%) with PVYNTN isolates from Poland, UK and Syria. ZYMV sequences showed maximum nucleotide identity (97.9%) with an isolate from Israel. The low genetic diversity amongst Iraqi potyviruses, alongside their high sequence identities with potyviruses from other countries, suggests a relatively recent introduction to Iraq through imported planting materials.
ISSN:1125-4653
2239-7264
DOI:10.4454/JPP.V98I3.029