Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis

We have constructed a confocal fluorescence microscope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The methodology and the sensitivity of the set-up using enhanced green fluorescent protein are describ...

Full description

Saved in:
Bibliographic Details
Published in:Current science (Bangalore) 2004-07, Vol.87 (2), p.239-244
Main Authors: Sinha, Deepak Kumar, Bhattacharya, Dipanjan, Banerjee, Bidisha, Hameed, Feroz Meeran, Shivashankar, G. V.
Format: Article
Language:English
Subjects:
Anisotropy
DNA
Fluorescence
Lasers
Messenger RNA
Microscopes
Molecules
Nucleotides
Polymerization
RESEARCH COMMUNICATIONS
Spectroscopy
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 244
container_issue 2
container_start_page 239
container_title Current science (Bangalore)
container_volume 87
creator Sinha, Deepak Kumar
Bhattacharya, Dipanjan
Banerjee, Bidisha
Hameed, Feroz Meeran
Shivashankar, G. V.
description We have constructed a confocal fluorescence microscope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The methodology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence correlation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence anisotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression.
format article
fullrecord <record><control><sourceid>jstor</sourceid><recordid>TN_cdi_jstor_primary_24108872</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>24108872</jstor_id><sourcerecordid>24108872</sourcerecordid><originalsourceid>FETCH-LOGICAL-j175t-1554437fb7c5b3815cf21792fe998bb2b01689a6d811fe4c42dfed92399754753</originalsourceid><addsrcrecordid>eNotzMtKAzEYhuFZKFirlyDkBgI5NslS6hEKbnRdMpk_NWMmGZJ4mLu3UlcfPHy8Z92KEEox14ZedJe1joQwzohZdcsdfEHM8wSpoexRDekQAU85gvuMgFqx7uNoyOXks7MRTcGVXF2e4WhTHxIM6Du0d-RzcYDqDK6dDssfoQMkwPAzF6g15IRssnGpoV51597GCtf_u-7eHu5ft0949_L4vL3d4ZEq2TCVUgiufK-c7Lmm0nlGlWEejNF9z3pCN9rYzaAp9SCcYIOHwTBujJJCSb7ubk7dsbZc9nMJky3LnglKtFaM_wKyAFhf</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis</title><source>JSTOR</source><source>EZB Electronic Journals Library</source><creator>Sinha, Deepak Kumar ; Bhattacharya, Dipanjan ; Banerjee, Bidisha ; Hameed, Feroz Meeran ; Shivashankar, G. V.</creator><creatorcontrib>Sinha, Deepak Kumar ; Bhattacharya, Dipanjan ; Banerjee, Bidisha ; Hameed, Feroz Meeran ; Shivashankar, G. V.</creatorcontrib><description>We have constructed a confocal fluorescence microscope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The methodology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence correlation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence anisotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression.</description><identifier>ISSN: 0011-3891</identifier><language>eng</language><publisher>Current Science Association</publisher><subject>Anisotropy ; DNA ; Fluorescence ; Lasers ; Messenger RNA ; Microscopes ; Molecules ; Nucleotides ; Polymerization ; RESEARCH COMMUNICATIONS ; Spectroscopy</subject><ispartof>Current science (Bangalore), 2004-07, Vol.87 (2), p.239-244</ispartof><rights>2004 Current Science Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24108872$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24108872$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,786,790,58593,58826</link.rule.ids></links><search><creatorcontrib>Sinha, Deepak Kumar</creatorcontrib><creatorcontrib>Bhattacharya, Dipanjan</creatorcontrib><creatorcontrib>Banerjee, Bidisha</creatorcontrib><creatorcontrib>Hameed, Feroz Meeran</creatorcontrib><creatorcontrib>Shivashankar, G. V.</creatorcontrib><title>Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis</title><title>Current science (Bangalore)</title><description>We have constructed a confocal fluorescence microscope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The methodology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence correlation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence anisotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression.</description><subject>Anisotropy</subject><subject>DNA</subject><subject>Fluorescence</subject><subject>Lasers</subject><subject>Messenger RNA</subject><subject>Microscopes</subject><subject>Molecules</subject><subject>Nucleotides</subject><subject>Polymerization</subject><subject>RESEARCH COMMUNICATIONS</subject><subject>Spectroscopy</subject><issn>0011-3891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNotzMtKAzEYhuFZKFirlyDkBgI5NslS6hEKbnRdMpk_NWMmGZJ4mLu3UlcfPHy8Z92KEEox14ZedJe1joQwzohZdcsdfEHM8wSpoexRDekQAU85gvuMgFqx7uNoyOXks7MRTcGVXF2e4WhTHxIM6Du0d-RzcYDqDK6dDssfoQMkwPAzF6g15IRssnGpoV51597GCtf_u-7eHu5ft0949_L4vL3d4ZEq2TCVUgiufK-c7Lmm0nlGlWEejNF9z3pCN9rYzaAp9SCcYIOHwTBujJJCSb7ubk7dsbZc9nMJky3LnglKtFaM_wKyAFhf</recordid><startdate>20040725</startdate><enddate>20040725</enddate><creator>Sinha, Deepak Kumar</creator><creator>Bhattacharya, Dipanjan</creator><creator>Banerjee, Bidisha</creator><creator>Hameed, Feroz Meeran</creator><creator>Shivashankar, G. V.</creator><general>Current Science Association</general><scope/></search><sort><creationdate>20040725</creationdate><title>Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis</title><author>Sinha, Deepak Kumar ; Bhattacharya, Dipanjan ; Banerjee, Bidisha ; Hameed, Feroz Meeran ; Shivashankar, G. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j175t-1554437fb7c5b3815cf21792fe998bb2b01689a6d811fe4c42dfed92399754753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Anisotropy</topic><topic>DNA</topic><topic>Fluorescence</topic><topic>Lasers</topic><topic>Messenger RNA</topic><topic>Microscopes</topic><topic>Molecules</topic><topic>Nucleotides</topic><topic>Polymerization</topic><topic>RESEARCH COMMUNICATIONS</topic><topic>Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sinha, Deepak Kumar</creatorcontrib><creatorcontrib>Bhattacharya, Dipanjan</creatorcontrib><creatorcontrib>Banerjee, Bidisha</creatorcontrib><creatorcontrib>Hameed, Feroz Meeran</creatorcontrib><creatorcontrib>Shivashankar, G. V.</creatorcontrib><jtitle>Current science (Bangalore)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sinha, Deepak Kumar</au><au>Bhattacharya, Dipanjan</au><au>Banerjee, Bidisha</au><au>Hameed, Feroz Meeran</au><au>Shivashankar, G. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis</atitle><jtitle>Current science (Bangalore)</jtitle><date>2004-07-25</date><risdate>2004</risdate><volume>87</volume><issue>2</issue><spage>239</spage><epage>244</epage><pages>239-244</pages><issn>0011-3891</issn><abstract>We have constructed a confocal fluorescence microscope combined with force measurements. Our method allows for simultaneous measurements of fluorescence anisotropy, energy transfer and correlation. The methodology and the sensitivity of the set-up using enhanced green fluorescent protein are described. We present results on (a) the detection of mRNA polymerization during in vitro transcription using fluorescence correlation spectroscopy and anisotropy, (b) detection of in vivo protein-DNA interactions using fluorescence anisotropy and (c) nanomanipulation of polytene chromosomes using the micropippete force sensor. Such a combined method allows for probing novel structure–function relationship underlying gene-expression.</abstract><pub>Current Science Association</pub><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0011-3891
ispartof Current science (Bangalore), 2004-07, Vol.87 (2), p.239-244
issn 0011-3891
language eng
recordid cdi_jstor_primary_24108872
source JSTOR; EZB Electronic Journals Library
subjects Anisotropy
DNA
Fluorescence
Lasers
Messenger RNA
Microscopes
Molecules
Nucleotides
Polymerization
RESEARCH COMMUNICATIONS
Spectroscopy
title Development of single-molecule tracking confocal microscope combined with force spectroscopy for gene-expression analysis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T01%3A54%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20single-molecule%20tracking%20confocal%20microscope%20combined%20with%20force%20spectroscopy%20for%20gene-expression%20analysis&rft.jtitle=Current%20science%20(Bangalore)&rft.au=Sinha,%20Deepak%20Kumar&rft.date=2004-07-25&rft.volume=87&rft.issue=2&rft.spage=239&rft.epage=244&rft.pages=239-244&rft.issn=0011-3891&rft_id=info:doi/&rft_dat=%3Cjstor%3E24108872%3C/jstor%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-j175t-1554437fb7c5b3815cf21792fe998bb2b01689a6d811fe4c42dfed92399754753%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_jstor_id=24108872&rfr_iscdi=true