Synthesis and use of pHEMA microbeads with human EA.hy 926 endothelial cells

Cancer has become a major problem in public health and the resulting bone metastases a worsening factor. Facing it, different strategies have been proposed and mechanisms involved in tumor angiogenesis are being studied. Enhanced permeability retention (EPR) effect is a key step in designing new ant...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2009-05, Vol.89B (2), p.501-507
Main Authors: Nyangoga, Hervé, Zecheru, Teodora, Filmon, Robert, Baslé, Michel-Félix, Cincu, Corneliu, Chappard, Daniel
Format: Article
Language:English
Subjects:
Biocompatible Materials
Cell Line
Drug Carriers - chemistry
drug delivery
endothelial cells
Endothelial Cells - cytology
Endothelial Cells - metabolism
EPR effect
Humans
Life Sciences
Materials Testing
Methacrylates - chemistry
Microspheres
Molecular Structure
Neoplasms - therapy
Neovascularization, Pathologic
pHEMA
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Summary:Cancer has become a major problem in public health and the resulting bone metastases a worsening factor. Facing it, different strategies have been proposed and mechanisms involved in tumor angiogenesis are being studied. Enhanced permeability retention (EPR) effect is a key step in designing new anticancer drugs. We have prepared poly 2‐hydroxyethyl methacrylate (pHEMA) microbeads to target human endothelial EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells. Microbeads were synthesized by emulsion precipitation method and carried positive or negative charges. EA.hy 926 cells were cultured in 24‐well plates and microbeads were deposited on cells at various times. Scanning and transmission electron microscopy, flow cytometry, confocal microscopy, and three‐dimensional (3D) reconstruction were used to characterize microbeads and their location outside and inside cells. Microbeads were uptaken by endothelial cells with a better internalization for negatively charged microbeads. 3D reconstruction of confocal optical sections clearly evidenced the uptake and internalization of microbeads by endothelial cells. pHEMA microbeads could represent potential drug carrier in tumor model of metastases. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
ISSN:1552-4973
1552-4981
1552-4981
DOI:10.1002/jbm.b.31240