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Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp
Spatial and temporal control of ovine prion protein ( Prnp) gene expression was achieved in mice using two transgenes: a Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine Pr...
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| Published in: | FEBS letters 2008-06, Vol.582 (15), p.2219-2224 |
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| Main Authors: | , , , , , , , , , , , , , , , , , |
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| container_end_page | 2224 |
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| creator | Gallozzi, Micaela Béringue, Vincent Decaunes, Pauline Le Dur, Annick Le Roux, Karine Tilly, Gaëlle Le Guillou, Sandrine Herzog, Laetitia Peyre, Coralie Ladroue, Aline Chapuis, Jérôme Vilotte, Marthe Passet, Bruno Costa, José Chenais, Nathalie Le Provost, Fabienne Laude, Hubert Vilotte, Jean-Luc |
| description | Spatial and temporal control of ovine prion protein (
Prnp) gene expression was achieved in mice using two transgenes: a
Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrP
C expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo. |
| doi_str_mv | 10.1016/j.febslet.2008.05.014 |
| format | article |
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Prnp) gene expression was achieved in mice using two transgenes: a
Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrP
C expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2008.05.014</identifier><identifier>PMID: 18501713</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Cellular Biology ; cellular prion protein ; chimeric tetracycline repressor that recruits histone deacetylases ; Down-Regulation ; Gene Expression ; Gene regulation ; Inducible promoter ; Life Sciences ; Mice ; Mice, Transgenic ; Neurons - metabolism ; Prion ; PrPC ; PrPC Proteins - genetics ; PrPSc ; scrapie-associated PrP ; Sheep - genetics ; Silencer Elements, Transcriptional ; tetracycline trans-activator ; Transgenes ; Transgenic mice ; transmissible spongiform encephalopathies ; TRSID ; TSE ; tTA</subject><ispartof>FEBS letters, 2008-06, Vol.582 (15), p.2219-2224</ispartof><rights>2008 Federation of European Biochemical Societies</rights><rights>FEBS Letters 582 (2008) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540X-26fca19a0e5ffc452a8c4ece9a5872bf468f58395ff23ca24b32c83275ff75c13</citedby><cites>FETCH-LOGICAL-c540X-26fca19a0e5ffc452a8c4ece9a5872bf468f58395ff23ca24b32c83275ff75c13</cites><orcidid>0000-0001-6706-5712 ; 0000-0003-4623-0584 ; 0000-0002-0588-5333 ; 0000-0002-0349-0047</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2008.05.014$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.febslet.2008.05.014$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,786,790,891,1424,27957,27958,45609,45610,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18501713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02667679$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallozzi, Micaela</creatorcontrib><creatorcontrib>Béringue, Vincent</creatorcontrib><creatorcontrib>Decaunes, Pauline</creatorcontrib><creatorcontrib>Le Dur, Annick</creatorcontrib><creatorcontrib>Le Roux, Karine</creatorcontrib><creatorcontrib>Tilly, Gaëlle</creatorcontrib><creatorcontrib>Le Guillou, Sandrine</creatorcontrib><creatorcontrib>Herzog, Laetitia</creatorcontrib><creatorcontrib>Peyre, Coralie</creatorcontrib><creatorcontrib>Ladroue, Aline</creatorcontrib><creatorcontrib>Chapuis, Jérôme</creatorcontrib><creatorcontrib>Vilotte, Marthe</creatorcontrib><creatorcontrib>Passet, Bruno</creatorcontrib><creatorcontrib>Costa, José</creatorcontrib><creatorcontrib>Chenais, Nathalie</creatorcontrib><creatorcontrib>Le Provost, Fabienne</creatorcontrib><creatorcontrib>Laude, Hubert</creatorcontrib><creatorcontrib>Vilotte, Jean-Luc</creatorcontrib><title>Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Spatial and temporal control of ovine prion protein (
Prnp) gene expression was achieved in mice using two transgenes: a
Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrP
C expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.</description><subject>Animals</subject><subject>Cellular Biology</subject><subject>cellular prion protein</subject><subject>chimeric tetracycline repressor that recruits histone deacetylases</subject><subject>Down-Regulation</subject><subject>Gene Expression</subject><subject>Gene regulation</subject><subject>Inducible promoter</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Neurons - metabolism</subject><subject>Prion</subject><subject>PrPC</subject><subject>PrPC Proteins - genetics</subject><subject>PrPSc</subject><subject>scrapie-associated PrP</subject><subject>Sheep - genetics</subject><subject>Silencer Elements, Transcriptional</subject><subject>tetracycline trans-activator</subject><subject>Transgenes</subject><subject>Transgenic mice</subject><subject>transmissible spongiform encephalopathies</subject><subject>TRSID</subject><subject>TSE</subject><subject>tTA</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqNUk1v1DAQtRCILoWfAPIJiUOCP-LE4YKW0rKVVgKxRerN8jqTrVdZO7WTfvz7OsoKjuVkzZs3b8bzBqH3lOSU0PLzPm9hGzsYckaIzInICS1eoAWVFc94UcqXaEESlImq5ifoTYx7kmJJ69fohEpBaEX5AoVNrwerO6xdgwc49D6koPH3LguwG7uU9A77Fg9Bu7gDBxge-gAxTvgYrdvh4Qbw1e_N5fcs2g6cgYCtwwdr4Ate9n1nzawyePwruP4tetXqLsK743uK_lycX52tsvXPH5dny3VmREGuM1a2RtNaExBtawrBtDQFGKi1kBXbtumLrZC8TlnGjWbFljMjOasSUAlD-Sn6NOve6E71wR50eFReW7VartWEEVaWVVnVdxP348ztg78dIQ7qYKOBrtMO_BhVWTPKi7p6lkjrIu2bsUQUM9EEH2OA9u8IlKjJQbVXRwfV5KAiQiW7Ut2HY4Nxe4DmX9XRskRYzYT7tOzH_1NVF-ff2GY6h-kaiCSkoOV1kvo6S0Gy4c5CUNHYycDGBjCDarx9ZtonCKbFPw</recordid><startdate>20080625</startdate><enddate>20080625</enddate><creator>Gallozzi, Micaela</creator><creator>Béringue, Vincent</creator><creator>Decaunes, Pauline</creator><creator>Le Dur, Annick</creator><creator>Le Roux, Karine</creator><creator>Tilly, Gaëlle</creator><creator>Le Guillou, Sandrine</creator><creator>Herzog, Laetitia</creator><creator>Peyre, Coralie</creator><creator>Ladroue, Aline</creator><creator>Chapuis, Jérôme</creator><creator>Vilotte, Marthe</creator><creator>Passet, Bruno</creator><creator>Costa, José</creator><creator>Chenais, Nathalie</creator><creator>Le Provost, Fabienne</creator><creator>Laude, Hubert</creator><creator>Vilotte, Jean-Luc</creator><general>Elsevier B.V</general><general>Wiley</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-6706-5712</orcidid><orcidid>https://orcid.org/0000-0003-4623-0584</orcidid><orcidid>https://orcid.org/0000-0002-0588-5333</orcidid><orcidid>https://orcid.org/0000-0002-0349-0047</orcidid></search><sort><creationdate>20080625</creationdate><title>Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp</title><author>Gallozzi, Micaela ; Béringue, Vincent ; Decaunes, Pauline ; Le Dur, Annick ; Le Roux, Karine ; Tilly, Gaëlle ; Le Guillou, Sandrine ; Herzog, Laetitia ; Peyre, Coralie ; Ladroue, Aline ; Chapuis, Jérôme ; Vilotte, Marthe ; Passet, Bruno ; Costa, José ; Chenais, Nathalie ; Le Provost, Fabienne ; Laude, Hubert ; Vilotte, Jean-Luc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540X-26fca19a0e5ffc452a8c4ece9a5872bf468f58395ff23ca24b32c83275ff75c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Cellular Biology</topic><topic>cellular prion protein</topic><topic>chimeric tetracycline repressor that recruits histone deacetylases</topic><topic>Down-Regulation</topic><topic>Gene Expression</topic><topic>Gene regulation</topic><topic>Inducible promoter</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Neurons - metabolism</topic><topic>Prion</topic><topic>PrPC</topic><topic>PrPC Proteins - genetics</topic><topic>PrPSc</topic><topic>scrapie-associated PrP</topic><topic>Sheep - genetics</topic><topic>Silencer Elements, Transcriptional</topic><topic>tetracycline trans-activator</topic><topic>Transgenes</topic><topic>Transgenic mice</topic><topic>transmissible spongiform encephalopathies</topic><topic>TRSID</topic><topic>TSE</topic><topic>tTA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallozzi, Micaela</creatorcontrib><creatorcontrib>Béringue, Vincent</creatorcontrib><creatorcontrib>Decaunes, Pauline</creatorcontrib><creatorcontrib>Le Dur, Annick</creatorcontrib><creatorcontrib>Le Roux, Karine</creatorcontrib><creatorcontrib>Tilly, Gaëlle</creatorcontrib><creatorcontrib>Le Guillou, Sandrine</creatorcontrib><creatorcontrib>Herzog, Laetitia</creatorcontrib><creatorcontrib>Peyre, Coralie</creatorcontrib><creatorcontrib>Ladroue, Aline</creatorcontrib><creatorcontrib>Chapuis, Jérôme</creatorcontrib><creatorcontrib>Vilotte, Marthe</creatorcontrib><creatorcontrib>Passet, Bruno</creatorcontrib><creatorcontrib>Costa, José</creatorcontrib><creatorcontrib>Chenais, Nathalie</creatorcontrib><creatorcontrib>Le Provost, Fabienne</creatorcontrib><creatorcontrib>Laude, Hubert</creatorcontrib><creatorcontrib>Vilotte, Jean-Luc</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallozzi, Micaela</au><au>Béringue, Vincent</au><au>Decaunes, Pauline</au><au>Le Dur, Annick</au><au>Le Roux, Karine</au><au>Tilly, Gaëlle</au><au>Le Guillou, Sandrine</au><au>Herzog, Laetitia</au><au>Peyre, Coralie</au><au>Ladroue, Aline</au><au>Chapuis, Jérôme</au><au>Vilotte, Marthe</au><au>Passet, Bruno</au><au>Costa, José</au><au>Chenais, Nathalie</au><au>Le Provost, Fabienne</au><au>Laude, Hubert</au><au>Vilotte, Jean-Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2008-06-25</date><risdate>2008</risdate><volume>582</volume><issue>15</issue><spage>2219</spage><epage>2224</epage><pages>2219-2224</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Spatial and temporal control of ovine prion protein (
Prnp) gene expression was achieved in mice using two transgenes: a
Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrP
C expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>18501713</pmid><doi>10.1016/j.febslet.2008.05.014</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-6706-5712</orcidid><orcidid>https://orcid.org/0000-0003-4623-0584</orcidid><orcidid>https://orcid.org/0000-0002-0588-5333</orcidid><orcidid>https://orcid.org/0000-0002-0349-0047</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2008-06, Vol.582 (15), p.2219-2224 |
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| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02667679v1 |
| source | Wiley-Blackwell Journals; BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS; Wiley |
| subjects | Animals Cellular Biology cellular prion protein chimeric tetracycline repressor that recruits histone deacetylases Down-Regulation Gene Expression Gene regulation Inducible promoter Life Sciences Mice Mice, Transgenic Neurons - metabolism Prion PrPC PrPC Proteins - genetics PrPSc scrapie-associated PrP Sheep - genetics Silencer Elements, Transcriptional tetracycline trans-activator Transgenes Transgenic mice transmissible spongiform encephalopathies TRSID TSE tTA |
| title | Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp |
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