Latrophilin participates in insecticide susceptibility through positively regulating CSP10 and partially compensated by OBPC01 in Tribolium castaneum

Latrophilin (LPH) is an adhesion G protein-coupled receptor (aGPCR) that participates in multiple essential physiological processes. Our previous studies have shown that lph is not only indispensable for the development and reproduction of red flour beetles (Tribolium castaneum), but also for their...

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Published in:Pesticide biochemistry and physiology 2019-09, Vol.159, p.107-117
Main Authors: Xiong, Wenfeng, Gao, Shanshan, Lu, Yaoyao, Wei, Luting, Mao, Jinjuan, Xie, Jia, Cao, Quanquan, Liu, Juanjuan, Bi, Jingxiu, Song, Xiaowen, Li, Bin
Format: Article
Language:English
Subjects:
Animals
Biodiversity and Ecology
Carbofuran
Dichlorvos
Ecology, environment
Ecosystems
Environmental Sciences
Gene Expression Regulation, Developmental - drug effects
Gene Expression Regulation, Developmental - genetics
Insect Proteins - genetics
Insect Proteins - metabolism
Insecticide susceptibility
Insecticides - pharmacology
Latrophilin
Life Sciences
Receptors, Peptide - metabolism
RNA interference
Tribolium - drug effects
Tribolium - metabolism
Tribolium castaneum
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Summary:Latrophilin (LPH) is an adhesion G protein-coupled receptor (aGPCR) that participates in multiple essential physiological processes. Our previous studies have shown that lph is not only indispensable for the development and reproduction of red flour beetles (Tribolium castaneum), but also for their resistance against dichlorvos or carbofuran insecticides. However, the regulatory mechanism of lph-mediated insecticide susceptibility remains unclear. Here, we revealed that knockdown of lph in beetles resulted in opposing changes in two chemoreception genes, chemosensory protein 10 (CSP10) and odorant-binding protein C01 (OBPC01), in which the expression of TcCSP10 was downregulated, whereas the expression of TcOBPC01 was upregulated. TcCSP10 and TcOBPC01 were expressed at the highest levels in early pupal and late larval stages, respectively. High levels of expression of both these genes were observed in the heads (without antennae) of adults. TcCSP10 and TcOBPC01 were significantly induced by dichlorvos or carbofuran between 12 and 72 h (hrs) after exposure, suggesting that they are likely associated with increasing the binding affinity of insecticides, leading to a decrease in sensitivity to the insecticides. Moreover, once these two genes were knocked down, the susceptibility of the beetles to dichlorvos or carbofuran was enhanced. Additionally, RNA interference (RNAi) targeting of lph followed by exposure to dichlorvos or carbofuran also caused the opposing expression levels of TcCSP10 and TcOBPC01 compared to the expression levels of wild-type larvae treated with insecticides alone. All these results indicate that lph is involved in insecticide susceptibility through positively regulating TcCSP10; and the susceptibility could also further partially compensated for through the negative regulation of TcOBPC01 when lph was knockdown in the red flour beetle. Our studies shed new light on the molecular regulatory mechanisms of lph related to insecticide susceptibility. lph was involved in insecticide susceptibility through positively regulating TcCSP10, and it could further partially compensate for insecticide susceptibility by negatively regulating TcOBPC01 when lph was knockdown in Tribolium castaneum. [Display omitted] •TcCSP10 was down-regulated and TcOBPC01 was up-regulated after knockdown of Tclph.•TcCSP10 and TcOBPC01 were significantly induced by insecticides of dichlorvos or carbofuran.•RNAi of TcCSP10 and TcOBPC01 increased the susceptibilities of l
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2019.06.005