Jr-ZFP2, encoding a Cys2/His2-type transcription factor, is involved in the early stages of the mechano-perception pathway and specifically expressed in mechanically stimulated tissues in woody plants

Plants respond to environmental mechanical stimulation, such as wind, by modifying their growth and development. To study the molecular effects of stem bending on 3-week-old walnut trees, a cDNA-AFLP approach was developed. This study allowed the identification of a cDNA, known as Jr-ZFP2, encoding...

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Published in:Plant, cell and environment cell and environment, 2008-06, Vol.31 (6), p.715-726
Main Authors: LEBLANC-FOURNIER, NATHALIE, COUTAND, CATHERINE, CROUZET, JEROME, BRUNEL, NICOLE, LENNE, CATHERINE, MOULIA, BRUNO, JULIEN, JEAN-LOUIS
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological and medical sciences
Biomechanical Phenomena
Cell physiology
Cloning, Molecular
Cys2/His2‐type zinc finger
DNA, Complementary - genetics
DNA, Plant - genetics
Ecology, environment
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - physiology
Juglans - genetics
Juglans - metabolism
Life Sciences
mechanical solicitation
Molecular and cellular biology
Molecular Sequence Data
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Stems - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal transduction
thigmomorphogenesis
Time
Transcription Factors - genetics
Transcription Factors - metabolism
tree
walnut
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Summary:Plants respond to environmental mechanical stimulation, such as wind, by modifying their growth and development. To study the molecular effects of stem bending on 3-week-old walnut trees, a cDNA-AFLP approach was developed. This study allowed the identification of a cDNA, known as Jr-ZFP2, encoding a Cys2/His2-type two-zinc-fingered transcription factor. Reverse transcriptase-polymerase chain reaction analysis confirmed that Jr-ZFP2 mRNA accumulation is rapidly and transiently induced after mechanical stimulation. After bending, Jr-ZFP2 transcript increase was restricted to the stem, the organ where the mechanical solicitation was applied. Furthermore, other abiotic factors, such as cold or salt, did not modify Jr-ZFP2 mRNA accumulation in walnut stems under our experimental conditions, whereas growth studies demonstrated that salt stress was actually perceived by the plants. These results suggest that the regulation of Jr-ZFP2 expression is more sensitive to mechanical stimulus. This gene will be a good marker for studying the early stages of mechanical perception in woody plants.
ISSN:0140-7791
1365-3040
DOI:10.1111/j.1365-3040.2008.01785.x