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Novel synergistic interactions between monolaurin, a mono-acyl glycerol and [beta] lactam antibiotics against Staphylococcus aureus: an in vitro study
A major worldwide health issue is the rising frequency of resistance of bacteria.Drug combinations are a winning strategy in fighting resistant bacteria and might help in protecting the existing drugs.Monolaurin is natural compound extracted from coconut oil and has a promising antimicrobial activit...
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| Published in: | BMC infectious diseases 2024-04, Vol.24 (1) |
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| creator | Ghany, Shimaa Salah Hassan Abd El Ibrahem, Reham A EL-Gendy, Ahmed Osama El-Baky, Rehab Mahmoud Abd Mustafa, Ahmad Azmy, Ahmed Farag |
| description | A major worldwide health issue is the rising frequency of resistance of bacteria.Drug combinations are a winning strategy in fighting resistant bacteria and might help in protecting the existing drugs.Monolaurin is natural compound extracted from coconut oil and has a promising antimicrobial activity against Staphylococcus.aureus. This study aims to examine the efficacy of monolaurin both individually and in combination with [beta]-lactam antibiotics against Staphylococcus aureus isolates. Agar dilution method was used for determination of minimum inhibitory concentration (MIC) of monolaurin against S.aureus isolates. Scanning electron microscope (SEM) was used to detect morphological changes in S.aureus after treatment with monolaurin. Conventional and Real-time Polymerase chain reaction (RT-PCR) were performed to detect of beta-lactamase (blaZ) gene and its expressional levels after monolaurin treatment. Combination therapy of monolaurin and antibiotics was assessed through fractional inhibitory concentration and time-kill method. The antibacterial activity of monolaurin was assessed on 115 S.aureus isolates, the MIC of monolaurin were 250 to 2000 [micro]g/ml. SEM showed cell elongation and swelling in the outer membrane of S.aureus in the prescence of 1xMIC of monolaurin. blaZ gene was found in 73.9% of S.aureus isolates. RT-PCR shows a significant decrease in of blaZ gene expression at 250 and 500 [micro]g/ml of monolaurin. Synergistic effects were detected through FIC method and time killing curve. Combination therapy established a significant reduction on the MIC value. The collective findings from the antibiotic combinations with monolaurin indicated synergism rates ranging from 83.3% to 100%.In time-kill studies, combination of monolaurin and [beta]-lactam antibiotics produced a synergistic effect. This study showed that monolaurin may be a natural antibacterial agent against S. aureus, and may be an outstanding modulator of [beta]-lactam drugs. The concurrent application of monolaurin and [beta]-lactam antibiotics, exhibiting synergistic effects against S. aureus in vitro, holds promise as potential candidates for the development of combination therapies that target particularly, patients with bacterial infections that are nearly incurable. |
| doi_str_mv | 10.1186/s12879-024-09261-9 |
| format | article |
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This study aims to examine the efficacy of monolaurin both individually and in combination with [beta]-lactam antibiotics against Staphylococcus aureus isolates. Agar dilution method was used for determination of minimum inhibitory concentration (MIC) of monolaurin against S.aureus isolates. Scanning electron microscope (SEM) was used to detect morphological changes in S.aureus after treatment with monolaurin. Conventional and Real-time Polymerase chain reaction (RT-PCR) were performed to detect of beta-lactamase (blaZ) gene and its expressional levels after monolaurin treatment. Combination therapy of monolaurin and antibiotics was assessed through fractional inhibitory concentration and time-kill method. The antibacterial activity of monolaurin was assessed on 115 S.aureus isolates, the MIC of monolaurin were 250 to 2000 [micro]g/ml. SEM showed cell elongation and swelling in the outer membrane of S.aureus in the prescence of 1xMIC of monolaurin. blaZ gene was found in 73.9% of S.aureus isolates. RT-PCR shows a significant decrease in of blaZ gene expression at 250 and 500 [micro]g/ml of monolaurin. Synergistic effects were detected through FIC method and time killing curve. Combination therapy established a significant reduction on the MIC value. The collective findings from the antibiotic combinations with monolaurin indicated synergism rates ranging from 83.3% to 100%.In time-kill studies, combination of monolaurin and [beta]-lactam antibiotics produced a synergistic effect. This study showed that monolaurin may be a natural antibacterial agent against S. aureus, and may be an outstanding modulator of [beta]-lactam drugs. The concurrent application of monolaurin and [beta]-lactam antibiotics, exhibiting synergistic effects against S. aureus in vitro, holds promise as potential candidates for the development of combination therapies that target particularly, patients with bacterial infections that are nearly incurable.</description><identifier>ISSN: 1471-2334</identifier><identifier>EISSN: 1471-2334</identifier><identifier>DOI: 10.1186/s12879-024-09261-9</identifier><language>eng</language><publisher>BioMed Central Ltd</publisher><subject>Antibiotics ; Bacteria ; Bacterial infections ; Beta lactam antibiotics ; Beta lactamases ; Coconut oil ; Drug resistance in microorganisms ; Drug synergism ; Drug therapy ; Gene expression ; Genes ; Glycerin ; Glycerol ; Health aspects ; Imipenem ; Materia medica, Vegetable ; Methicillin ; Monoglycerides ; Pharmaceutical research ; Plant extracts ; Staphylococcus aureus ; Staphylococcus aureus infections ; Testing</subject><ispartof>BMC infectious diseases, 2024-04, Vol.24 (1)</ispartof><rights>COPYRIGHT 2024 BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids></links><search><creatorcontrib>Ghany, Shimaa Salah Hassan Abd El</creatorcontrib><creatorcontrib>Ibrahem, Reham A</creatorcontrib><creatorcontrib>EL-Gendy, Ahmed Osama</creatorcontrib><creatorcontrib>El-Baky, Rehab Mahmoud Abd</creatorcontrib><creatorcontrib>Mustafa, Ahmad</creatorcontrib><creatorcontrib>Azmy, Ahmed Farag</creatorcontrib><title>Novel synergistic interactions between monolaurin, a mono-acyl glycerol and [beta] lactam antibiotics against Staphylococcus aureus: an in vitro study</title><title>BMC infectious diseases</title><description>A major worldwide health issue is the rising frequency of resistance of bacteria.Drug combinations are a winning strategy in fighting resistant bacteria and might help in protecting the existing drugs.Monolaurin is natural compound extracted from coconut oil and has a promising antimicrobial activity against Staphylococcus.aureus. This study aims to examine the efficacy of monolaurin both individually and in combination with [beta]-lactam antibiotics against Staphylococcus aureus isolates. Agar dilution method was used for determination of minimum inhibitory concentration (MIC) of monolaurin against S.aureus isolates. Scanning electron microscope (SEM) was used to detect morphological changes in S.aureus after treatment with monolaurin. Conventional and Real-time Polymerase chain reaction (RT-PCR) were performed to detect of beta-lactamase (blaZ) gene and its expressional levels after monolaurin treatment. Combination therapy of monolaurin and antibiotics was assessed through fractional inhibitory concentration and time-kill method. The antibacterial activity of monolaurin was assessed on 115 S.aureus isolates, the MIC of monolaurin were 250 to 2000 [micro]g/ml. SEM showed cell elongation and swelling in the outer membrane of S.aureus in the prescence of 1xMIC of monolaurin. blaZ gene was found in 73.9% of S.aureus isolates. RT-PCR shows a significant decrease in of blaZ gene expression at 250 and 500 [micro]g/ml of monolaurin. Synergistic effects were detected through FIC method and time killing curve. Combination therapy established a significant reduction on the MIC value. The collective findings from the antibiotic combinations with monolaurin indicated synergism rates ranging from 83.3% to 100%.In time-kill studies, combination of monolaurin and [beta]-lactam antibiotics produced a synergistic effect. This study showed that monolaurin may be a natural antibacterial agent against S. aureus, and may be an outstanding modulator of [beta]-lactam drugs. The concurrent application of monolaurin and [beta]-lactam antibiotics, exhibiting synergistic effects against S. aureus in vitro, holds promise as potential candidates for the development of combination therapies that target particularly, patients with bacterial infections that are nearly incurable.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Beta lactam antibiotics</subject><subject>Beta lactamases</subject><subject>Coconut oil</subject><subject>Drug resistance in microorganisms</subject><subject>Drug synergism</subject><subject>Drug therapy</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glycerin</subject><subject>Glycerol</subject><subject>Health aspects</subject><subject>Imipenem</subject><subject>Materia medica, Vegetable</subject><subject>Methicillin</subject><subject>Monoglycerides</subject><subject>Pharmaceutical research</subject><subject>Plant extracts</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus infections</subject><subject>Testing</subject><issn>1471-2334</issn><issn>1471-2334</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNj8tKxTAQhosoeH0BVwFXgtUkbXNxJ-INRMHbRuQwTdIayUmkSdW-iM9rUBcKLmQWM_Pz_XMpik2CdwkRbC8SKrgsMa1LLCkjpVwoVkjNSUmrql78US8XqzE-YUy4oHKleL8IL8ahOHkz9DYmq5D1yQygkg0-otakV2M8mgcfHIyD9TsIPrsS1ORQ7yZlhuAQeI3uMw0PyGUzzLOSbGtDHhkR9GB9TOg6wfPj5IIKSo1ZHgczxv2M5q3oxaYhoJhGPa0XSx24aDa-81pxe3x0c3hanl-enB0enJc9qZomnyAM62opsaAt5cBbTlXNGGBKhAatsCbQGC01NNzwFgMmhDWsE0wzxWS1Vmx9ze3BmZn1XUj59bmNanbAhayIIHWTqd0_qBzazK0K3nQ2678M278MmUnmLfUwxjg7u776P3t595P9ANmyl98</recordid><startdate>20240408</startdate><enddate>20240408</enddate><creator>Ghany, Shimaa Salah Hassan Abd El</creator><creator>Ibrahem, Reham A</creator><creator>EL-Gendy, Ahmed Osama</creator><creator>El-Baky, Rehab Mahmoud Abd</creator><creator>Mustafa, Ahmad</creator><creator>Azmy, Ahmed Farag</creator><general>BioMed Central Ltd</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20240408</creationdate><title>Novel synergistic interactions between monolaurin, a mono-acyl glycerol and [beta] lactam antibiotics against Staphylococcus aureus: an in vitro study</title><author>Ghany, Shimaa Salah Hassan Abd El ; Ibrahem, Reham A ; EL-Gendy, Ahmed Osama ; El-Baky, Rehab Mahmoud Abd ; Mustafa, Ahmad ; Azmy, Ahmed Farag</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1355-ac8e6f499082b27a7b72c466a0218dadc0d1a5ed9da57e7b0a011656f86d6c693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Beta lactam antibiotics</topic><topic>Beta lactamases</topic><topic>Coconut oil</topic><topic>Drug resistance in microorganisms</topic><topic>Drug synergism</topic><topic>Drug therapy</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Glycerin</topic><topic>Glycerol</topic><topic>Health aspects</topic><topic>Imipenem</topic><topic>Materia medica, Vegetable</topic><topic>Methicillin</topic><topic>Monoglycerides</topic><topic>Pharmaceutical research</topic><topic>Plant extracts</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus infections</topic><topic>Testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghany, Shimaa Salah Hassan Abd El</creatorcontrib><creatorcontrib>Ibrahem, Reham A</creatorcontrib><creatorcontrib>EL-Gendy, Ahmed Osama</creatorcontrib><creatorcontrib>El-Baky, Rehab Mahmoud Abd</creatorcontrib><creatorcontrib>Mustafa, Ahmad</creatorcontrib><creatorcontrib>Azmy, Ahmed Farag</creatorcontrib><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><jtitle>BMC infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghany, Shimaa Salah Hassan Abd El</au><au>Ibrahem, Reham A</au><au>EL-Gendy, Ahmed Osama</au><au>El-Baky, Rehab Mahmoud Abd</au><au>Mustafa, Ahmad</au><au>Azmy, Ahmed Farag</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel synergistic interactions between monolaurin, a mono-acyl glycerol and [beta] lactam antibiotics against Staphylococcus aureus: an in vitro study</atitle><jtitle>BMC infectious diseases</jtitle><date>2024-04-08</date><risdate>2024</risdate><volume>24</volume><issue>1</issue><issn>1471-2334</issn><eissn>1471-2334</eissn><abstract>A major worldwide health issue is the rising frequency of resistance of bacteria.Drug combinations are a winning strategy in fighting resistant bacteria and might help in protecting the existing drugs.Monolaurin is natural compound extracted from coconut oil and has a promising antimicrobial activity against Staphylococcus.aureus. This study aims to examine the efficacy of monolaurin both individually and in combination with [beta]-lactam antibiotics against Staphylococcus aureus isolates. Agar dilution method was used for determination of minimum inhibitory concentration (MIC) of monolaurin against S.aureus isolates. Scanning electron microscope (SEM) was used to detect morphological changes in S.aureus after treatment with monolaurin. Conventional and Real-time Polymerase chain reaction (RT-PCR) were performed to detect of beta-lactamase (blaZ) gene and its expressional levels after monolaurin treatment. Combination therapy of monolaurin and antibiotics was assessed through fractional inhibitory concentration and time-kill method. The antibacterial activity of monolaurin was assessed on 115 S.aureus isolates, the MIC of monolaurin were 250 to 2000 [micro]g/ml. SEM showed cell elongation and swelling in the outer membrane of S.aureus in the prescence of 1xMIC of monolaurin. blaZ gene was found in 73.9% of S.aureus isolates. RT-PCR shows a significant decrease in of blaZ gene expression at 250 and 500 [micro]g/ml of monolaurin. Synergistic effects were detected through FIC method and time killing curve. Combination therapy established a significant reduction on the MIC value. The collective findings from the antibiotic combinations with monolaurin indicated synergism rates ranging from 83.3% to 100%.In time-kill studies, combination of monolaurin and [beta]-lactam antibiotics produced a synergistic effect. This study showed that monolaurin may be a natural antibacterial agent against S. aureus, and may be an outstanding modulator of [beta]-lactam drugs. The concurrent application of monolaurin and [beta]-lactam antibiotics, exhibiting synergistic effects against S. aureus in vitro, holds promise as potential candidates for the development of combination therapies that target particularly, patients with bacterial infections that are nearly incurable.</abstract><pub>BioMed Central Ltd</pub><doi>10.1186/s12879-024-09261-9</doi></addata></record> |
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| subjects | Antibiotics Bacteria Bacterial infections Beta lactam antibiotics Beta lactamases Coconut oil Drug resistance in microorganisms Drug synergism Drug therapy Gene expression Genes Glycerin Glycerol Health aspects Imipenem Materia medica, Vegetable Methicillin Monoglycerides Pharmaceutical research Plant extracts Staphylococcus aureus Staphylococcus aureus infections Testing |
| title | Novel synergistic interactions between monolaurin, a mono-acyl glycerol and [beta] lactam antibiotics against Staphylococcus aureus: an in vitro study |
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